Q9P7G5 (BOS1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein transport protein bos1

Gene names

Name:	bos1
ORF Names:	SPAP14E8.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	SNARE required for targeting and fusion of ER-derived transport vesicles with the Golgi complex By similarity.
Subunit structure	Component of a SNARE complex consisting of sed5, bos1, bet1 and sec22 or ykt6. Interacts with YIF1 and YIP1 By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type IV membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the BOS1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from sequence orthology. Source: PomBase intracellular protein transport Inferred from sequence orthology. Source: PomBase
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 16823372. Source: PomBase Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro endoplasmic reticulum membrane Inferred from sequence orthology. Source: PomBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	SNAP receptor activity Inferred from sequence orthology. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Protein transport protein bos1		PRO_0000207555
Regions
	Topological domain	1 – 213	213	Cytoplasmic Potential
	Transmembrane	214 – 234	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	235	1	Vesicular Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9P7G5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 559F4F10AD58785C	FASTA	235	26,855
	10 20 30 40 50 60 MSNTLYNHCT KLFQSIQRSL DELERGVQDE SNTISLAGIQ GQISASFLSL SRSIDDYDSM 70 80 90 100 110 120 VQRELVPAKK KKATIRIQEF RQKHVQLLEK FDELKAHVRD IAQAKNRKEL LGRRGYVNSL 130 140 150 160 170 180 DSPYGNSTTD AEIVEGPSDL SRQDGLLKEH DFLGRAESQV DEFLERGRMI LGDLVEQGSV 190 200 210 220 230 LKATKTKVLN AANTLGITRH TLSLINRRSK QDKIIFYCGA FLVFVLFYLI YRWLR

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB77004.1.
RefSeq	NP_593539.1. NM_001018973.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAP14E8.03-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAP14E8.03.1; SPAP14E8.03.1:pep; SPAP14E8.03.
GeneID	2541965.
KEGG	spo:SPAP14E8.03.
Organism-specific databases
PomBase	SPAP14E8.03.
Phylogenomic databases
eggNOG	NOG148687.
HOGENOM	HOG000217458.
KO	K08496.
OMA	AKQDKWL.
OrthoDB	EOG4W3WXH.
Family and domain databases
InterPro	IPR027027. GOSR2/Membrin/Bos1. [Graphical view]
PIRSF	PIRSF028865. Membrin-2. 1 hit.
ProtoNet	Search...
Other
NextBio	20803048.

Entry information

Entry name

BOS1_SCHPO

Accession

Primary (citable) accession number: Q9P7G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program