Reviewed, UniProtKB/Swiss-Prot Q9P7F2 (AMOH2_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative primary amine oxidase 2
    EC=1.4.3.21
Alternative name(s):
    Copper amine oxidase 2

Gene names

ORF Names:

SPAC2E1P3.04

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	712 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.
Cofactor	Binds 1 copper ion per subunit By similarity. Contains 1 topaquinone per subunit By similarity.
Subcellular location	Cytoplasm. Ref.2
Post-translational modification	Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue By similarity.
Sequence similarities	Belongs to the copper/topaquinone oxidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	TPQ
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amine catabolic process Inferred from direct assay. Source: GeneDB_SPombe copper ion homeostasis Inferred from mutant phenotype. Source: GeneDB_SPombe oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	amine oxidase activity Inferred from direct assay. Source: GeneDB_SPombe copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: GeneDB_SPombe quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 712	712	Putative primary amine oxidase 2		PRO_0000316036
Sites
	Active site	321	1	Proton acceptor By similarity
	Active site	407	1	Schiff-base intermediate with substrate; via topaquinone By similarity
	Metal binding	458	1	Copper By similarity
	Metal binding	460	1	Copper By similarity
	Metal binding	627	1	Copper By similarity
Amino acid modifications
	Modified residue	407	1	2',4',5'-topaquinone By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P7F2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E48DE1CBA0D1C084	FASTA	712	81,241
	10 20 30 40 50 60 MAYYPHLPYH HHHHTSVPGE RLSDPLDPLS ADELKLAVEI IRHEYPSKHF AFNVVTLEEP 70 80 90 100 110 120 PKAKYLHWKY SKEDAHKPER IALAVLLEKG VPGILEARVN LTKAEVIQIE HITGVCPILT 130 140 150 160 170 180 ADMLVNTEQI VRKDPAVIEQ CILSGVPPDQ MDHVYCDPWT IGYDERYGNT RRMQQAMMYY 190 200 210 220 230 240 RSNEDDSQYS HPLDFCPIID TEDQKVVAID IPPVRRPLSK HKHSNFNKKD IEAELGKMRE 250 260 270 280 290 300 VKPISVTQPE GVNFRMKGRY IEWQNFRMHI GFNYREGIVL SDISYNDNGH IRPLFYRMSI 310 320 330 340 350 360 AEMVVPYGNP EHPHQRKHAF DLGEYGAGYL TNPLALGCDC KGVIHYLDAH FVNNTGEVET 370 380 390 400 410 420 VKNAICIHEE DDGVLFKHSD FRDKFRTTIS ARGIRLVISQ IFTAANYEYM VYWIFHMDGV 430 440 450 460 470 480 IECELKLTGI LNTYAMNEGE DLKGWGTQVY PQVNAHNHQH LFCLRLNPML DGYSNSVAVV 490 500 510 520 530 540 DGVSGPGEPG SKENYYGNAF TTERTVPKTV KEAICDYNSD TSRTWDICNP NKLHPYSGKP 550 560 570 580 590 600 VSYKLVSRET PRLMARPGSL VSNRAGFARH HIHVTPYKDG QIYPAGDYVP QTSGEPTKGL 610 620 630 640 650 660 PEWIAEEPDA SVDNTDIVVW HTFGITHFPA PEDFPLMPAE PIRLLLRPRN FFLRNPALDV 670 680 690 700 710 PPSKNVTTSE VKQAHHHGNL HMMDMMKSLT DATSEFAFGE KFCEKHKGDH FF

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB83008.1.
RefSeq	NP_593985.1.
3D structure databases
HSSP	HSSP built from PDB template 1EKM based on UniProtKB P12807.
ModBase	Search...
Genome annotation databases
GeneID	2541754.
KEGG	spo:SPAC2E1P3.04.
NMPDR	fig|4896.1.peg.3955.
Organism-specific databases
GeneDB_Spombe	SPAC2E1P3.04.
Phylogenomic databases
OMA	Q9P7F2. ITHFPAP.
Enzyme and pathway databases
BRENDA	1.4.3.6. 653.
Gene expression databases
ArrayExpress	Q9P7F2.
Family and domain databases
InterPro	IPR000269. Cu_amine_oxidase. IPR015798. Cu_amine_oxidase_C. IPR015800. Cu_amine_oxidase_N2. IPR015801. Cu_amine_oxidase_N2/3. IPR015802. Cu_amine_oxidase_N3. [Graphical view]
Gene3D	G3DSA:3.10.450.40. CuNH_oxidase. 2 hits. G3DSA:2.70.98.20. Lyase_8_central. 1 hit.
PANTHER	PTHR10638. CuNH_oxidase. 1 hit.
Pfam	PF01179. Cu_amine_oxid. 1 hit. PF02727. Cu_amine_oxidN2. 1 hit. PF02728. Cu_amine_oxidN3. 1 hit. [Graphical view]
PROSITE	PS01164. COPPER_AMINE_OXID_1. 1 hit. PS01165. COPPER_AMINE_OXID_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMOH2_SCHPO

Accession

Primary (citable) accession number: Q9P7F2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)