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Q9P7D7 (LCF2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase 2

EC=6.2.1.3
Alternative name(s):
Fatty acid activator 2
Long-chain acyl-CoA synthetase 2
Gene names
Name:lcf2
ORF Names:SPBP4H10.11c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Plays an important role in the determination of viability in the stationary phase. Ref.3

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactor

Magnesium By similarity.

Subcellular location

Golgi apparatus. Vacuole membrane; Peripheral membrane protein Ref.2.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689Long-chain-fatty-acid--CoA ligase 2
PRO_0000311761

Sequences

Sequence LengthMass (Da)Tools
Q9P7D7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 5F80C06F6EA92F87

FASTA68975,791
        10         20         30         40         50         60 
MDIKDYYCVE TKDSKQPGES YIYRSIHSAG KLLDTPEDGV STVYDLLCTA AKNHGEKQAM 

        70         80         90        100        110        120 
GSRKLIKENR EEREIIRKVD NEEVVEKKLW TTYELGPYEY ISFNKVYEIA LALGSGLVAS 

       130        140        150        160        170        180 
GITSETTMLF FAATSAKWFT TAQGCSSQAI PIVTAYETLG EDGIYTSLDE CKSRAIFTDP 

       190        200        210        220        230        240 
NLIPKLLGPL KQSTWVKLIV CSSTPSEDLV ELVKSTAPDV EIITYDNLLS LGKEKPQPPH 

       250        260        270        280        290        300 
PPKADDICCY MYTSGSTGKP KGVVLLHRNI IAALGGINRI LSQHINVKDY VLAYLPLAHI 

       310        320        330        340        350        360 
FEFIFEMCCL YWGGVLGYAS PRTLTDASVV NCKGDLTEFR PTVLIGVPAV YELIKKGILA 

       370        380        390        400        410        420 
KVSSMPAHRQ KVFSGSLSLK QYLIERNLPG SAALDAIVFN KVKAATGGRL RYCISGGAAL 

       430        440        450        460        470        480 
AASTQAFLSS CICPVLPGYG LTETCAGSFV LSPEQWHLYA NTVGFPIPSI EFKLVDIPDL 

       490        500        510        520        530        540 
GYYTDSSPPR GEVWIRGPAV CNGYLNRPED NKAAFTEDGW FKTGDVGEIA KGNTLRLIDR 

       550        560        570        580        590        600 
KKNIVKSLNG EYIALEKIEA QFFTSPLVSN VCAYADVNHA KPVVIVNPDE NGLRTYLTKN 

       610        620        630        640        650        660 
SGSSFNGNPN DTLTNLCKDS GVQHLILKEL INIGKQQRLA SIEIPEGVVL SDFEWTAENN 

       670        680 
FLTASRKVKR QVIVAHYSDE IQKAYSKKH 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]"Identification of a fatty acyl-CoA synthetase gene, lcf2+, which affects viability after entry into the stationary phase in Schizosaccharomyces pombe."
Fujita Y., Mita S., Ohtsuka H., Aiba H.
Biosci. Biotechnol. Biochem. 71:3041-3047(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU329671 Genomic DNA. Translation: CAB83169.1.
RefSeqNP_596185.1. NM_001022104.2.

3D structure databases

ProteinModelPortalQ9P7D7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277861. 8 interactions.
IntActQ9P7D7. 1 interaction.
MINTMINT-4705249.
STRING4896.SPBP4H10.11c-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBP4H10.11c.1; SPBP4H10.11c.1:pep; SPBP4H10.11c.
GeneID2541350.
KEGGspo:SPBP4H10.11c.

Organism-specific databases

PomBaseSPBP4H10.11c.

Phylogenomic databases

eggNOGCOG1022.
HOGENOMHOG000159459.
KOK01897.
OMAHPRTLSE.
OrthoDBEOG7CCC0K.
PhylomeDBQ9P7D7.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20802459.
PROQ9P7D7.

Entry information

Entry nameLCF2_SCHPO
AccessionPrimary (citable) accession number: Q9P7D7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names