Reviewed, UniProtKB/Swiss-Prot Q9P7D7 (LCF2_SCHPO)

Names and origin

Protein names

Recommended name:
    Long-chain-fatty-acid--CoA ligase 2
    EC=6.2.1.3
Alternative name(s):
    Long-chain acyl-CoA synthetase 2
    Fatty acid activator 2

Gene names

ORF Names:

SPBP4H10.11c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium By similarity.
Subcellular location	Golgi apparatus. Vacuole membrane; Peripheral membrane protein Ref.2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Golgi apparatus Membrane Vacuole
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	long-chain fatty acid metabolic process Inferred from genetic interaction. Source: GeneDB_SPombe
Cellular component	Golgi apparatus Ref.2 Inferred from direct assay. Source: GeneDB_SPombe extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole membrane Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain-fatty-acid-CoA ligase activity Inferred from genetic interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Long-chain-fatty-acid--CoA ligase 2		PRO_0000311761

Sequences

	Sequence		Length	Mass (Da)
	Q9P7D7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5F80C06F6EA92F87	FASTA	689	75,791
	10 20 30 40 50 60 MDIKDYYCVE TKDSKQPGES YIYRSIHSAG KLLDTPEDGV STVYDLLCTA AKNHGEKQAM 70 80 90 100 110 120 GSRKLIKENR EEREIIRKVD NEEVVEKKLW TTYELGPYEY ISFNKVYEIA LALGSGLVAS 130 140 150 160 170 180 GITSETTMLF FAATSAKWFT TAQGCSSQAI PIVTAYETLG EDGIYTSLDE CKSRAIFTDP 190 200 210 220 230 240 NLIPKLLGPL KQSTWVKLIV CSSTPSEDLV ELVKSTAPDV EIITYDNLLS LGKEKPQPPH 250 260 270 280 290 300 PPKADDICCY MYTSGSTGKP KGVVLLHRNI IAALGGINRI LSQHINVKDY VLAYLPLAHI 310 320 330 340 350 360 FEFIFEMCCL YWGGVLGYAS PRTLTDASVV NCKGDLTEFR PTVLIGVPAV YELIKKGILA 370 380 390 400 410 420 KVSSMPAHRQ KVFSGSLSLK QYLIERNLPG SAALDAIVFN KVKAATGGRL RYCISGGAAL 430 440 450 460 470 480 AASTQAFLSS CICPVLPGYG LTETCAGSFV LSPEQWHLYA NTVGFPIPSI EFKLVDIPDL 490 500 510 520 530 540 GYYTDSSPPR GEVWIRGPAV CNGYLNRPED NKAAFTEDGW FKTGDVGEIA KGNTLRLIDR 550 560 570 580 590 600 KKNIVKSLNG EYIALEKIEA QFFTSPLVSN VCAYADVNHA KPVVIVNPDE NGLRTYLTKN 610 620 630 640 650 660 SGSSFNGNPN DTLTNLCKDS GVQHLILKEL INIGKQQRLA SIEIPEGVVL SDFEWTAENN 670 680 FLTASRKVKR QVIVAHYSDE IQKAYSKKH

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB83169.1.
RefSeq	NP_596185.1.
3D structure databases
SMR	Q9P7D7. Positions 34-680.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P7D7. 1 interaction.
STRING	Q9P7D7.
Genome annotation databases
GeneID	2541350.
KEGG	spo:SPBP4H10.11c.
NMPDR	fig|4896.1.peg.2051.
Organism-specific databases
GeneDB_Spombe	SPBP4H10.11c.
Phylogenomic databases
eggNOG	fuNOG04652.
HOGENOM	HBG721674.
OMA	GVAIICN.
OrthoDB	EOG9JM95Z.
PhylomeDB	Q9P7D7.
Enzyme and pathway databases
BRENDA	6.2.1.3. 653.
Gene expression databases
ArrayExpress	Q9P7D7.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCF2_SCHPO

Accession

Primary (citable) accession number: Q9P7D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)