Q9P7D7 (LCF2_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Long-chain-fatty-acid--CoA ligase 2
EC=6.2.1.3

Alternative name(s):
Fatty acid activator 2
Long-chain acyl-CoA synthetase 2

Gene names

ORF Names:

SPBP4H10.11c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.
Catalytic activity	ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor	Magnesium By similarity.
Subcellular location	Golgi apparatus. Vacuole membrane; Peripheral membrane protein Ref.2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Golgi apparatus Membrane Vacuole
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from sequence or structural similarity. Source: GeneDB_Spombe long-chain fatty acid metabolic process Inferred from genetic interaction. Source: GeneDB_Spombe negative regulation of G0 to G1 transition Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	Golgi apparatus Inferred from direct assay. Source: GeneDB_Spombe fungal-type vacuole membrane Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW long-chain fatty acid-CoA ligase activity Inferred from genetic interaction. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Long-chain-fatty-acid--CoA ligase 2		PRO_0000311761

Sequences

	Sequence		Length	Mass (Da)
	Q9P7D7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5F80C06F6EA92F87	FASTA	689	75,791
	10 20 30 40 50 60 MDIKDYYCVE TKDSKQPGES YIYRSIHSAG KLLDTPEDGV STVYDLLCTA AKNHGEKQAM 70 80 90 100 110 120 GSRKLIKENR EEREIIRKVD NEEVVEKKLW TTYELGPYEY ISFNKVYEIA LALGSGLVAS 130 140 150 160 170 180 GITSETTMLF FAATSAKWFT TAQGCSSQAI PIVTAYETLG EDGIYTSLDE CKSRAIFTDP 190 200 210 220 230 240 NLIPKLLGPL KQSTWVKLIV CSSTPSEDLV ELVKSTAPDV EIITYDNLLS LGKEKPQPPH 250 260 270 280 290 300 PPKADDICCY MYTSGSTGKP KGVVLLHRNI IAALGGINRI LSQHINVKDY VLAYLPLAHI 310 320 330 340 350 360 FEFIFEMCCL YWGGVLGYAS PRTLTDASVV NCKGDLTEFR PTVLIGVPAV YELIKKGILA 370 380 390 400 410 420 KVSSMPAHRQ KVFSGSLSLK QYLIERNLPG SAALDAIVFN KVKAATGGRL RYCISGGAAL 430 440 450 460 470 480 AASTQAFLSS CICPVLPGYG LTETCAGSFV LSPEQWHLYA NTVGFPIPSI EFKLVDIPDL 490 500 510 520 530 540 GYYTDSSPPR GEVWIRGPAV CNGYLNRPED NKAAFTEDGW FKTGDVGEIA KGNTLRLIDR 550 560 570 580 590 600 KKNIVKSLNG EYIALEKIEA QFFTSPLVSN VCAYADVNHA KPVVIVNPDE NGLRTYLTKN 610 620 630 640 650 660 SGSSFNGNPN DTLTNLCKDS GVQHLILKEL INIGKQQRLA SIEIPEGVVL SDFEWTAENN 670 680 FLTASRKVKR QVIVAHYSDE IQKAYSKKH

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB83169.1.
RefSeq	NP_596185.1. NM_001022104.1.
3D structure databases
ProteinModelPortal	Q9P7D7.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P7D7. 1 interaction.
STRING	Q9P7D7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBP4H10.11c.1; SPBP4H10.11c.1:pep; SPBP4H10.11c.
GeneID	2541350.
KEGG	spo:SPBP4H10.11c.
NMPDR	fig|4896.1.peg.2051.
Organism-specific databases
GeneDB_Spombe	SPBP4H10.11c.
Phylogenomic databases
eggNOG	fuNOG04652.
GeneTree	EFGT00050000001161.
HOGENOM	HBG721674.
OMA	SHACASI.
OrthoDB	EOG4TF3TN.
Gene expression databases
ArrayExpress	Q9P7D7.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01897.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCF2_SCHPO

Accession

Primary (citable) accession number: Q9P7D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program