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Q9P7D4

- ACON2_SCHPO

UniProt

Q9P7D4 - ACON2_SCHPO

Protein

Homocitrate dehydratase, mitochondrial

Gene

SPBP4H10.15

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 3 (11 Jul 2012)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible dehydration of (R)-homocitrate to cis-homoaconitate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.By similarity

    Catalytic activityi

    (R)-2-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O.

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051SubstrateBy similarity
    Metal bindingi394 – 3941Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi457 – 4571Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi460 – 4601Iron-sulfur (4Fe-4S)By similarity
    Binding sitei484 – 4841SubstrateBy similarity
    Binding sitei489 – 4891SubstrateBy similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. aconitate hydratase activity Source: PomBase
    3. metal ion binding Source: UniProtKB-KW
    4. structural constituent of ribosome Source: PomBase

    GO - Biological processi

    1. glutamate metabolic process Source: PomBase
    2. lysine biosynthetic process via aminoadipic acid Source: UniProtKB-UniPathway
    3. mitochondrial translation Source: PomBase
    4. tricarboxylic acid cycle Source: PomBase

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00033; UER00029.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homocitrate dehydratase, mitochondrial (EC:4.2.1.-)
    Alternative name(s):
    Aconitase 2
    Gene namesi
    ORF Names:SPBP4H10.15
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBP4H10.15.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrial large ribosomal subunit Source: PomBase
    2. mitochondrion Source: PomBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionSequence AnalysisAdd
    BLAST
    Chaini31 – 918888Homocitrate dehydratase, mitochondrialPRO_0000316198Add
    BLAST

    Proteomic databases

    MaxQBiQ9P7D4.
    PaxDbiQ9P7D4.

    Interactioni

    Protein-protein interaction databases

    BioGridi277859. 6 interactions.
    MINTiMINT-4705221.
    STRINGi4896.SPBP4H10.15-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P7D4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni198 – 2003Substrate bindingBy similarity
    Regioni680 – 6812Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1048.
    HOGENOMiHOG000224293.
    KOiK17450.
    OrthoDBiEOG7X6M7Q.

    Family and domain databases

    Gene3Di3.20.19.10. 1 hit.
    3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015928. Aconitase/3IPM_dehydase_swvl.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    IPR006248. Aconitase_mito-like.
    IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
    IPR028909. L21p-like.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    PF00694. Aconitase_C. 1 hit.
    PF00829. Ribosomal_L21p. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF141091. SSF141091. 1 hit.
    SSF52016. SSF52016. 1 hit.
    SSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR01340. aconitase_mito. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9P7D4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATFARMKLC LSGSSQAIPS KGISLVAARF QSTASRASYV TPPYEKLMGK    50
    LQQVRKFLPG QKLTLAEKVL YSHLVNPEES FSGVSPSDIR GSLYLKLNPD 100
    RVAMQDASAQ MALLQFMTCG LEKTMIPASI HCDHLIVGHR GANSDIPDSI 150
    ANNKEIFDFL QSAAKKYGIQ FWGPGSGIIH QIVLENYAAP GGMMLGTDSH 200
    TPNAGGLGMI AIGVGGADAV DAMTNTPWEL KAPKIIGVNL TGAMSGWTTP 250
    KDLILHLAGK LTVRGGTGHI IEYFGPGVAS LSCTGMATVC NMGAEVGATT 300
    SIFPYTDSMR RYLIATHRAE VADAASEVHS EYNYLAADTG AKYDQIIDIN 350
    LSELTPSLNG PFTPDLSTPV SKFGEAIEKN KWPKKLSAGL IGSCTNSSYQ 400
    DMTCVVDVVE QAISAGLKPK VPFLVTPGSE QIRATIERDG ITERLEEAGA 450
    TVLANACGPC IGMWKRTDDI ASGEPNAILT SFNRNFRSRN DGNPSTMNFL 500
    TSPVIVAAKI FSSDLAFDPT HDTLQTPDGK AFKFRPPQGV ELPSAGFIAG 550
    DSSYIPEPNP QPVPETEVTI DPKSDRLEAL EPFEPYQGGE MENLKVAVKV 600
    KGKCTTDHIS AAGKWLKYKG HLSNICNNTL IGAMNAATGE VNRAYDNGKG 650
    MTIPELMWKW KKDGQPWLVV AEHNYGEGSA REHAALQPRA MNGRIILTKS 700
    FARIHETNLK KQGVLPLTFV NEADYEKIDA EDKVSTRGIE QLLEGVLDQP 750
    ITLVVTKKDG SVVEIPCKHT MSKDQIEFFK AGSALNLIRE KAHSGVVNQK 800
    VIDSIKQQPD HYADAYIFNR HFVIAKGDQL GLPFHLKGVQ VGDTIRLDKI 850
    ASFGSRDFTL FGNPYVDPSL FTIEAVVLSF PKSALSVRVK HKRRHRHDRV 900
    MKHKQTYTIL RVTELKLN 918
    Length:918
    Mass (Da):99,901
    Last modified:July 11, 2012 - v3
    Checksum:iA17A3132B7B21D90
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAB83173.3.
    RefSeqiNP_596189.3. NM_001022108.3.

    Genome annotation databases

    EnsemblFungiiSPBP4H10.15.1; SPBP4H10.15.1:pep; SPBP4H10.15.
    GeneIDi2541348.
    KEGGispo:SPBP4H10.15.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329671 Genomic DNA. Translation: CAB83173.3 .
    RefSeqi NP_596189.3. NM_001022108.3.

    3D structure databases

    ProteinModelPortali Q9P7D4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277859. 6 interactions.
    MINTi MINT-4705221.
    STRINGi 4896.SPBP4H10.15-1.

    Proteomic databases

    MaxQBi Q9P7D4.
    PaxDbi Q9P7D4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBP4H10.15.1 ; SPBP4H10.15.1:pep ; SPBP4H10.15 .
    GeneIDi 2541348.
    KEGGi spo:SPBP4H10.15.

    Organism-specific databases

    PomBasei SPBP4H10.15.

    Phylogenomic databases

    eggNOGi COG1048.
    HOGENOMi HOG000224293.
    KOi K17450.
    OrthoDBi EOG7X6M7Q.

    Enzyme and pathway databases

    UniPathwayi UPA00033 ; UER00029 .

    Miscellaneous databases

    NextBioi 20802457.

    Family and domain databases

    Gene3Di 3.20.19.10. 1 hit.
    3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    InterProi IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015928. Aconitase/3IPM_dehydase_swvl.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    IPR006248. Aconitase_mito-like.
    IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
    IPR028909. L21p-like.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    PF00694. Aconitase_C. 1 hit.
    PF00829. Ribosomal_L21p. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF141091. SSF141091. 1 hit.
    SSF52016. SSF52016. 1 hit.
    SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR01340. aconitase_mito. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Comparative functional genomics of the fission yeasts."
      Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
      , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
      Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiACON2_SCHPO
    AccessioniPrimary (citable) accession number: Q9P7D4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: July 11, 2012
    Last modified: October 1, 2014
    This is version 92 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3