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Q9P7C3

- MNS1_SCHPO

UniProt

Q9P7C3 - MNS1_SCHPO

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Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

SPAC2E1P5.01c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in glycoprotein quality control as it is important for the targeting of misfolded glycoproteins for degradation. It trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2 with low efficiency.1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

Cofactori

Ca2+By similarity

Enzyme regulationi

Inhibited by kifunensine.1 Publication

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: PomBase

GO - Biological processi

  1. misfolded or incompletely synthesized glycoprotein catabolic process Source: PomBase
  2. protein deglycosylation involved in glycoprotein catabolic process Source: PomBase
  3. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_188628. ER Quality Control Compartment (ERQC).

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113)
Alternative name(s):
ER alpha-1,2-mannosidase
Man(9)-alpha-mannosidase
Gene namesi
ORF Names:SPAC2E1P5.01c, SPAPB1E7.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC2E1P5.01c.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 3123Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini32 – 521490LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. endoplasmic reticulum Source: PomBase
  3. endoplasmic reticulum membrane Source: PomBase
  4. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidasePRO_0000210320Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi330 ↔ 373By similarity
Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9P7C3.

Interactioni

Protein-protein interaction databases

MINTiMINT-4705108.
STRINGi4896.SPAC2E1P5.01c-1.

Structurei

3D structure databases

ProteinModelPortaliQ9P7C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
HOGENOMiHOG000181987.
InParanoidiQ9P7C3.
KOiK01230.
OMAiTHEHTIN.
OrthoDBiEOG75TMND.
PhylomeDBiQ9P7C3.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P7C3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVKRRTVKYF LRRILALFVL CVPIYYLYTT VQRPPGYTKL KGSTRRKALI
60 70 80 90 100
KKTFIESWTD YETYGWGKDE YYPIIKRGRN YLRKGMGWMI IDSLDTMMIM
110 120 130 140 150
GLDEQVLRAR EWVNNSLTWN QDDEEVSVFE TTIRILGGLL SSYHLSQDKL
160 170 180 190 200
YLDRAVDLAD RLLAAYNTST GLPRSNVNLG TRKSRKRTRE YFVSTAESGT
210 220 230 240 250
VQMELRYLSY LTGDPKYWIT ADKTMEVLLG DATWSHTGLV PITVNLITGA
260 270 280 290 300
YVGRNIRLGS HGDSYYEYLL KQDLQLFSSG TVYRKAFDLS VDGIIEYLLN
310 320 330 340 350
YTTPNHFAYI AELPGGLEHA QLPKMDHLVC FLPGTLMWGA TNGTSLEAAR
360 370 380 390 400
TSKNWGTRQE RDVKLAQELM RTCYEMYNMT ATGLAPEIVF FDVDQTKNEI
410 420 430 440 450
YSKRRDQHNL MRPETVESLF ILYRITRDEI YREWGWNIFV SFLRYSRLPG
460 470 480 490 500
RDAFTCLDSV ESKKVKDQRD KTESFWFAET LKYLYLLFED DFSILPLTNY
510 520
TFNTEAHPFP NIENNMDLYT V
Length:521
Mass (Da):60,813
Last modified:March 29, 2004 - v2
Checksum:i742E325D05EC8936
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAC36930.1.
RefSeqiNP_594139.2. NM_001019563.2.

Genome annotation databases

EnsemblFungiiSPAC2E1P5.01c.1; SPAC2E1P5.01c.1:pep; SPAC2E1P5.01c.
GeneIDi2541719.
KEGGispo:SPAC2E1P5.01c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAC36930.1 .
RefSeqi NP_594139.2. NM_001019563.2.

3D structure databases

ProteinModelPortali Q9P7C3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4705108.
STRINGi 4896.SPAC2E1P5.01c-1.

Protein family/group databases

CAZyi GH47. Glycoside Hydrolase Family 47.

Proteomic databases

MaxQBi Q9P7C3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC2E1P5.01c.1 ; SPAC2E1P5.01c.1:pep ; SPAC2E1P5.01c .
GeneIDi 2541719.
KEGGi spo:SPAC2E1P5.01c.

Organism-specific databases

PomBasei SPAC2E1P5.01c.

Phylogenomic databases

eggNOGi NOG300315.
HOGENOMi HOG000181987.
InParanoidi Q9P7C3.
KOi K01230.
OMAi THEHTIN.
OrthoDBi EOG75TMND.
PhylomeDBi Q9P7C3.

Enzyme and pathway databases

Reactomei REACT_188628. ER Quality Control Compartment (ERQC).

Miscellaneous databases

NextBioi 20802810.
PROi Q9P7C3.

Family and domain databases

Gene3Di 1.50.10.50. 1 hit.
InterProi IPR001382. Glyco_hydro_47.
[Graphical view ]
PANTHERi PTHR11742. PTHR11742. 1 hit.
Pfami PF01532. Glyco_hydro_47. 1 hit.
[Graphical view ]
PRINTSi PR00747. GLYHDRLASE47.
SUPFAMi SSF48225. SSF48225. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Characterization of Schizosaccharomyces pombe ER alpha-mannosidase: a reevaluation of the role of the enzyme on ER-associated degradation."
    Movsichoff F., Castro O.A., Parodi A.J.
    Mol. Biol. Cell 16:4714-4724(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.

Entry informationi

Entry nameiMNS1_SCHPO
AccessioniPrimary (citable) accession number: Q9P7C3
Secondary accession number(s): Q9C0Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 29, 2004
Last modified: November 26, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3