Reviewed, UniProtKB/Swiss-Prot Q9P7C3 (YKU1_SCHPO)

Names and origin

Protein names

Recommended name:
    Putative mannosyl-oligosaccharide 1,2-alpha-mannosidase
    EC=3.2.1.113
Alternative name(s):
    Man(9)-alpha-mannosidase

Gene names

ORF Names:

SPAC2E1P5.01c, SPAPB1E7.13c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred by curator. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from mutant phenotype. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Putative mannosyl-oligosaccharide 1,2-alpha-mannosidase		PRO_0000210320
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 31	23	Signal-anchor for type II membrane protein Potential
	Topological domain	32 – 521	490	Lumenal Potential
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	378	1	N-linked (GlcNAc...) Potential
	Glycosylation	499	1	N-linked (GlcNAc...) Potential
	Disulfide bond	330 ↔ 373		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P7C3-1 [UniParc]. Last modified March 29, 2004. Version 2. Checksum: 742E325D05EC8936	FASTA	521	60,813
	10 20 30 40 50 60 MVKRRTVKYF LRRILALFVL CVPIYYLYTT VQRPPGYTKL KGSTRRKALI KKTFIESWTD 70 80 90 100 110 120 YETYGWGKDE YYPIIKRGRN YLRKGMGWMI IDSLDTMMIM GLDEQVLRAR EWVNNSLTWN 130 140 150 160 170 180 QDDEEVSVFE TTIRILGGLL SSYHLSQDKL YLDRAVDLAD RLLAAYNTST GLPRSNVNLG 190 200 210 220 230 240 TRKSRKRTRE YFVSTAESGT VQMELRYLSY LTGDPKYWIT ADKTMEVLLG DATWSHTGLV 250 260 270 280 290 300 PITVNLITGA YVGRNIRLGS HGDSYYEYLL KQDLQLFSSG TVYRKAFDLS VDGIIEYLLN 310 320 330 340 350 360 YTTPNHFAYI AELPGGLEHA QLPKMDHLVC FLPGTLMWGA TNGTSLEAAR TSKNWGTRQE 370 380 390 400 410 420 RDVKLAQELM RTCYEMYNMT ATGLAPEIVF FDVDQTKNEI YSKRRDQHNL MRPETVESLF 430 440 450 460 470 480 ILYRITRDEI YREWGWNIFV SFLRYSRLPG RDAFTCLDSV ESKKVKDQRD KTESFWFAET 490 500 510 520 LKYLYLLFED DFSILPLTNY TFNTEAHPFP NIENNMDLYT V

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAC36930.1.
RefSeq	NP_594139.2.
3D structure databases
SMR	Q9P7C3. Positions 42-516.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9P7C3.
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Genome annotation databases
GeneID	2541719.
KEGG	spo:SPAC2E1P5.01c.
Organism-specific databases
GeneDB_Spombe	SPAC2E1P5.01c.
Phylogenomic databases
eggNOG	fuNOG05524.
HOGENOM	HBG559734.
OrthoDB	EOG9DFR4W.
PhylomeDB	Q9P7C3.
Enzyme and pathway databases
BRENDA	3.2.1.113. 653.
Gene expression databases
ArrayExpress	Q9P7C3.
Family and domain databases
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
Gene3D	G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
PANTHER	PTHR11742. Glyco_hydro_47. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
ProtoNet	Search...

Entry information

Entry name

YKU1_SCHPO

Accession

Primary (citable) accession number: Q9P7C3
Secondary accession number(s): Q9C0Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	January 19, 2010
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)