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Q9P7C3

- MNS1_SCHPO

UniProt

Q9P7C3 - MNS1_SCHPO

Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

SPAC2E1P5.01c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (29 Mar 2004)
      Previous versions | rss
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    Functioni

    Involved in glycoprotein quality control as it is important for the targeting of misfolded glycoproteins for degradation. It trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2 with low efficiency.1 Publication

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.1 Publication

    Cofactori

    Calcium.By similarity

    Enzyme regulationi

    Inhibited by kifunensine.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: PomBase

    GO - Biological processi

    1. misfolded or incompletely synthesized glycoprotein catabolic process Source: PomBase
    2. protein deglycosylation involved in glycoprotein catabolic process Source: PomBase
    3. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_188628. ER Quality Control Compartment (ERQC).
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113)
    Alternative name(s):
    ER alpha-1,2-mannosidase
    Man(9)-alpha-mannosidase
    Gene namesi
    ORF Names:SPAC2E1P5.01c, SPAPB1E7.13c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC2E1P5.01c.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. endoplasmic reticulum Source: PomBase
    3. endoplasmic reticulum membrane Source: PomBase
    4. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 521521Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidasePRO_0000210320Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi330 ↔ 373By similarity
    Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ9P7C3.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4705108.
    STRINGi4896.SPAC2E1P5.01c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P7C3.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini32 – 521490LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3123Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    HOGENOMiHOG000181987.
    KOiK01230.
    OMAiTHEHTIN.
    OrthoDBiEOG75TMND.
    PhylomeDBiQ9P7C3.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9P7C3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKRRTVKYF LRRILALFVL CVPIYYLYTT VQRPPGYTKL KGSTRRKALI    50
    KKTFIESWTD YETYGWGKDE YYPIIKRGRN YLRKGMGWMI IDSLDTMMIM 100
    GLDEQVLRAR EWVNNSLTWN QDDEEVSVFE TTIRILGGLL SSYHLSQDKL 150
    YLDRAVDLAD RLLAAYNTST GLPRSNVNLG TRKSRKRTRE YFVSTAESGT 200
    VQMELRYLSY LTGDPKYWIT ADKTMEVLLG DATWSHTGLV PITVNLITGA 250
    YVGRNIRLGS HGDSYYEYLL KQDLQLFSSG TVYRKAFDLS VDGIIEYLLN 300
    YTTPNHFAYI AELPGGLEHA QLPKMDHLVC FLPGTLMWGA TNGTSLEAAR 350
    TSKNWGTRQE RDVKLAQELM RTCYEMYNMT ATGLAPEIVF FDVDQTKNEI 400
    YSKRRDQHNL MRPETVESLF ILYRITRDEI YREWGWNIFV SFLRYSRLPG 450
    RDAFTCLDSV ESKKVKDQRD KTESFWFAET LKYLYLLFED DFSILPLTNY 500
    TFNTEAHPFP NIENNMDLYT V 521
    Length:521
    Mass (Da):60,813
    Last modified:March 29, 2004 - v2
    Checksum:i742E325D05EC8936
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAC36930.1.
    RefSeqiNP_594139.2. NM_001019563.2.

    Genome annotation databases

    EnsemblFungiiSPAC2E1P5.01c.1; SPAC2E1P5.01c.1:pep; SPAC2E1P5.01c.
    GeneIDi2541719.
    KEGGispo:SPAC2E1P5.01c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAC36930.1 .
    RefSeqi NP_594139.2. NM_001019563.2.

    3D structure databases

    ProteinModelPortali Q9P7C3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4705108.
    STRINGi 4896.SPAC2E1P5.01c-1.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    Proteomic databases

    MaxQBi Q9P7C3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC2E1P5.01c.1 ; SPAC2E1P5.01c.1:pep ; SPAC2E1P5.01c .
    GeneIDi 2541719.
    KEGGi spo:SPAC2E1P5.01c.

    Organism-specific databases

    PomBasei SPAC2E1P5.01c.

    Phylogenomic databases

    eggNOGi NOG300315.
    HOGENOMi HOG000181987.
    KOi K01230.
    OMAi THEHTIN.
    OrthoDBi EOG75TMND.
    PhylomeDBi Q9P7C3.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_188628. ER Quality Control Compartment (ERQC).

    Miscellaneous databases

    NextBioi 20802810.
    PROi Q9P7C3.

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Characterization of Schizosaccharomyces pombe ER alpha-mannosidase: a reevaluation of the role of the enzyme on ER-associated degradation."
      Movsichoff F., Castro O.A., Parodi A.J.
      Mol. Biol. Cell 16:4714-4724(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.

    Entry informationi

    Entry nameiMNS1_SCHPO
    AccessioniPrimary (citable) accession number: Q9P7C3
    Secondary accession number(s): Q9C0Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: March 29, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3