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Q9P772 (GUAA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene names
Name:gua1
ORF Names:SPAP7G5.02c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathway

Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
   Biological processGMP biosynthesis
Purine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processGMP biosynthetic process

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytosol

Inferred from direct assay. Source: GeneDB_Spombe

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GMP synthase (glutamine-hydrolyzing) activity

Inferred from sequence or structural similarity. Source: GeneDB_Spombe

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 539539GMP synthase [glutamine-hydrolyzing]
PRO_0000286154

Regions

Domain20 – 215196Glutamine amidotransferase type-1
Domain216 – 413198GMPS ATP-PPase
Nucleotide binding244 – 2507ATP By similarity

Sites

Active site961For GATase activity By similarity
Active site1891For GATase activity By similarity
Active site1911For GATase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9P772 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: F83A1C4575F71723

FASTA53960,044
        10         20         30         40         50         60 
MSSTEVPGEV STSVSSYFDT ILILDFGSQY SHLIARRLRE IHVYAELLPC TQKIEALPFK 

        70         80         90        100        110        120 
PIGVILSGGP YSVYDDIAPH VDPAVFELGV PVLGICYGMQ EIAWLNGRCV EPGIEREYGP 

       130        140        150        160        170        180 
ATVSMEPEIK TEVFKSFFNS MPKEFEVWMS HGDRLSALPN GYETIGRTKN SPFAVIAHVT 

       190        200        210        220        230        240 
KPIIGLQFHP EVTHTPLGLQ LIKNFAIEIC HAKPNWSMEN FVDKEILRIR KMIGPSDHVI 

       250        260        270        280        290        300 
GAVSGGVDST VASKVLKEAI GDRFHAIMVD NGLLRLNEAE IVRETLNKHL GIQLTVVDAS 

       310        320        330        340        350        360 
EEFIGKLKGV TDPEKKRKII GNTFIHVFER EAERIVKETN GKVEYLLQGT LYPDVIESIS 

       370        380        390        400        410        420 
FKGPSQTIKT HHNVGGLLKD MKLKLIEPLR ELFKDEVRAL GELLGIEHSL VWRHPFPGPG 

       430        440        450        460        470        480 
LGIRILGEVN AAQLEIARKA DHIFITEIRN HGYYDKISQA FAALLPVKAV GVMGDKRTHE 

       490        500        510        520        530 
QVIALRAITT SDFMTADWYD GFSIKFLKLV SSRICNEVSG VNRVLYDISS KPPATVEME

« Hide

References

[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329670 Genomic DNA. Translation: CAB88269.1.
RefSeqNP_594312.1. NM_001019735.1.

3D structure databases

HSSPHSSP built from PDB template 1GPM based on UniProtKB P04079.
ProteinModelPortalQ9P772.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9P772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAP7G5.02c.1; SPAP7G5.02c.1:pep; SPAP7G5.02c.
GeneID2542573.
GenomeReviewsGene locus gua1 in contig CU329670_GR.
KEGGspo:SPAP7G5.02c.
NMPDRfig|4896.1.peg.4282.

Organism-specific databases

GeneDB_SpombeSPAP7G5.02c.

Phylogenomic databases

eggNOGfuNOG07551.
GeneTreeEFGT00050000004027.
HOGENOMHBG391775.
OMADGRTYEY.
OrthoDBEOG4WWVST.

Gene expression databases

ArrayExpressQ9P772.

Family and domain databases

InterProIPR017926. GATASE_1.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01951.
PfamPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR00884. GuaA_Cterm. 1 hit.
TIGR00888. GuaA_Nterm. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUAA_SCHPO
AccessionPrimary (citable) accession number: Q9P772
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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