Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial

Gene

tca-8

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation1 Publication
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (tca-8), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (tca-9)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77Coenzyme AUniRule annotation1
Binding sitei194Substrate; shared with subunit betaUniRule annotation1
Active sitei286Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandNucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit alphaUniRule annotation
Short name:
SCS-alphaUniRule annotation
Gene namesi
Name:tca-81 Publication
ORF Names:8D4.130, NCU01227
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 2, Linkage Group V

Organism-specific databases

EuPathDBiFungiDB:NCU01227

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 23MitochondrionUniRule annotationAdd BLAST23
ChainiPRO_000026016224 – 333Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrialUniRule annotationAdd BLAST310

Proteomic databases

PRIDEiQ9P727

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9P727
SMRiQ9P727
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 54Coenzyme A bindingUniRule annotation4
Regioni130 – 132Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000239685
InParanoidiQ9P727
KOiK01899
OMAiIIFVPPA
OrthoDBiEOG092C3IM6

Family and domain databases

Gene3Di3.40.50.261, 1 hit
HAMAPiMF_01988 Succ_CoA_alpha, 1 hit
InterProiView protein in InterPro
IPR017440 Cit_synth/succinyl-CoA_lig_AS
IPR033847 Citrt_syn/SCS-alpha_CS
IPR003781 CoA-bd
IPR005810 CoA_lig_alpha
IPR005811 CoA_ligase
IPR036291 NAD(P)-bd_dom_sf
IPR016102 Succinyl-CoA_synth-like
PfamiView protein in Pfam
PF02629 CoA_binding, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001553 SucCS_alpha, 1 hit
SMARTiView protein in SMART
SM00881 CoA_binding, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01019 sucCoAalpha, 1 hit
PROSITEiView protein in PROSITE
PS01216 SUCCINYL_COA_LIG_1, 1 hit
PS00399 SUCCINYL_COA_LIG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P727-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAIARQTRP AGKLVQQLSK RAYSSNSSPY AATINNLKIN GDTKVLFQGF
60 70 80 90 100
TGKQGTFHAQ QAIEYGTNVV GGTNPKKAGQ THLDRPVFSN VSEAVKETGA
110 120 130 140 150
TATAIFVPPP LAAAGIEEAL QAEIPLAVCI TEGIPQHDMV RITSMLKTQS
160 170 180 190 200
KTRLVGPNCP GIIAPGQCKI GIMPGFIHKR GRIGIVSRSG TLTYEAVNQT
210 220 230 240 250
TQAGLGQSLV VGIGGDPFSG TNFIDCLKVF LEDGDTDGII MIGEIGGSAE
260 270 280 290 300
EEAADFLKEY NTKNGGKPVV SFIAGISAPP GRRMGHAGAI VAGGKGGADS
310 320 330
KIKALEAAGV VVERSPASLG KALRDEFVRR DLL
Length:333
Mass (Da):34,738
Last modified:October 1, 2000 - v1
Checksum:iBA6FBF63AD918BDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353819 Genomic DNA Translation: CAB88555.1
CM002240 Genomic DNA Translation: EAA32357.1
PIRiT48739
RefSeqiXP_961593.1, XM_956500.3

Genome annotation databases

EnsemblFungiiEAA32357; EAA32357; NCU01227
GeneIDi3877725
KEGGincr:NCU01227

Entry informationi

Entry nameiSUCA_NEUCR
AccessioniPrimary (citable) accession number: Q9P727
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health