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Protein

Probable tRNA-splicing endonuclease subunit tsp-2

Gene

tsp-2

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei535 – 5351By similarity
Active sitei543 – 5431By similarity
Active sitei586 – 5861By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit tsp-2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen2
tRNA-splicing protein 2
Gene namesi
Name:tsp-2
Synonyms:sen2
ORF Names:13E11.340, NCU01115
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805 Componenti: Chromosome 2, Linkage Group V

Organism-specific databases

EuPathDBiFungiDB:NCU01115.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Probable tRNA-splicing endonuclease subunit tsp-2PRO_0000109460Add
BLAST

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-5/sen54 (By similarity).By similarity

Protein-protein interaction databases

STRINGi5141.EFNCRP00000004401.

Structurei

3D structure databases

ProteinModelPortaliQ9P6Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000159660.
InParanoidiQ9P6Y2.
KOiK15322.
OMAiWLHCVNR.
OrthoDBiEOG7SXWFH.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
[Graphical view]
PANTHERiPTHR21227:SF0. PTHR21227:SF0. 1 hit.
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P6Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENMTIATM AQPSESPSKS GEEAPASTNP AATSAPPRPP RVPYHQIYKL
60 70 80 90 100
PAPIRTFPLP TFYPSNPLSL FHLAVAWLRQ VLLPPPAEPS VIHEGIWDPD
110 120 130 140 150
TRSVHVKDPK SIRALWEQGF YGKGSLSRSE PNWFKRELSR RGLDGTTVSE
160 170 180 190 200
ERTASRREER RQVKWERAKA ELEAIEKQKH EEAKLNSETV NAAIPEPPAA
210 220 230 240 250
EIEPEPEVLL SSLPDHVLSA TSTIPLSRAA PTNEFVPKTV AHGHVEPKPP
260 270 280 290 300
VGPLELLALP NSYALVRGEE LALSQPEPAP TEENVRKELK APVGPIELLA
310 320 330 340 350
LPNSLVDLVA LSVVSVLSED AGVDEDESST EDQLEASALT NDEPETSATE
360 370 380 390 400
ANDSTSSSSP PHTDGLPDSQ PTQENVPATP ERRKSVRFSS TVESTTFQHT
410 420 430 440 450
DAASEVKNNN GSSDVEVFSS FVSAVEKPVI LNVEPTVISQ ELVDKEHFQL
460 470 480 490 500
APEEAFFLTF GLGALRVVDP VTEAPISNEQ LLIKLRANSY FPPRSVDNLS
510 520 530 540 550
PEDPFLVQYA VYHHFRSLGW VPRHGIKFGV DWIIYQRGPV FDHSEFGIMV
560 570 580 590 600
VPSFSDPRWS EFEHEESKKT WSWLMGVNRV LSHVLKSLVL VYVDVPPPPV
610 620 630
FDEEMKKGGI AAALKKFTIR EVMVRRFSVN RNR
Length:633
Mass (Da):70,104
Last modified:October 1, 2000 - v1
Checksum:iF7536C1E5E62563E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353820 Genomic DNA. Translation: CAB88602.1.
CM002240 Genomic DNA. Translation: EAA31786.1.
PIRiT48786.
RefSeqiXP_961022.1. XM_955929.3.

Genome annotation databases

EnsemblFungiiEFNCRT00000004401; EFNCRP00000004401; EFNCRG00000004395.
GeneIDi3877201.
KEGGincr:NCU01115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353820 Genomic DNA. Translation: CAB88602.1.
CM002240 Genomic DNA. Translation: EAA31786.1.
PIRiT48786.
RefSeqiXP_961022.1. XM_955929.3.

3D structure databases

ProteinModelPortaliQ9P6Y2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5141.EFNCRP00000004401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEFNCRT00000004401; EFNCRP00000004401; EFNCRG00000004395.
GeneIDi3877201.
KEGGincr:NCU01115.

Organism-specific databases

EuPathDBiFungiDB:NCU01115.

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000159660.
InParanoidiQ9P6Y2.
KOiK15322.
OMAiWLHCVNR.
OrthoDBiEOG7SXWFH.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
[Graphical view]
PANTHERiPTHR21227:SF0. PTHR21227:SF0. 1 hit.
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiSEN2_NEUCR
AccessioniPrimary (citable) accession number: Q9P6Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.