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Q9P6Y2

- SEN2_NEUCR

UniProt

Q9P6Y2 - SEN2_NEUCR

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Protein
Probable tRNA-splicing endonuclease subunit tsp-2
Gene
tsp-2, sen2, 13E11.340, NCU01115
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage By similarity.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei535 – 5351 By similarity
Active sitei543 – 5431 By similarity
Active sitei586 – 5861 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit tsp-2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen2
tRNA-splicing protein 2
Gene namesi
Name:tsp-2
Synonyms:sen2
ORF Names:13E11.340, NCU01115
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 2, Linkage Group V

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 633633Probable tRNA-splicing endonuclease subunit tsp-2
PRO_0000109460Add
BLAST

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-5/sen54 By similarity.

Protein-protein interaction databases

STRINGi5141.NCU01115.1.

Structurei

3D structure databases

ProteinModelPortaliQ9P6Y2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000159660.
KOiK15322.
OMAiWLHCVNR.
OrthoDBiEOG7SXWFH.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P6Y2-1 [UniParc]FASTAAdd to Basket

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MAENMTIATM AQPSESPSKS GEEAPASTNP AATSAPPRPP RVPYHQIYKL    50
PAPIRTFPLP TFYPSNPLSL FHLAVAWLRQ VLLPPPAEPS VIHEGIWDPD 100
TRSVHVKDPK SIRALWEQGF YGKGSLSRSE PNWFKRELSR RGLDGTTVSE 150
ERTASRREER RQVKWERAKA ELEAIEKQKH EEAKLNSETV NAAIPEPPAA 200
EIEPEPEVLL SSLPDHVLSA TSTIPLSRAA PTNEFVPKTV AHGHVEPKPP 250
VGPLELLALP NSYALVRGEE LALSQPEPAP TEENVRKELK APVGPIELLA 300
LPNSLVDLVA LSVVSVLSED AGVDEDESST EDQLEASALT NDEPETSATE 350
ANDSTSSSSP PHTDGLPDSQ PTQENVPATP ERRKSVRFSS TVESTTFQHT 400
DAASEVKNNN GSSDVEVFSS FVSAVEKPVI LNVEPTVISQ ELVDKEHFQL 450
APEEAFFLTF GLGALRVVDP VTEAPISNEQ LLIKLRANSY FPPRSVDNLS 500
PEDPFLVQYA VYHHFRSLGW VPRHGIKFGV DWIIYQRGPV FDHSEFGIMV 550
VPSFSDPRWS EFEHEESKKT WSWLMGVNRV LSHVLKSLVL VYVDVPPPPV 600
FDEEMKKGGI AAALKKFTIR EVMVRRFSVN RNR 633
Length:633
Mass (Da):70,104
Last modified:October 1, 2000 - v1
Checksum:iF7536C1E5E62563E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL353820 Genomic DNA. Translation: CAB88602.1.
CM002240 Genomic DNA. Translation: EAA31786.1.
PIRiT48786.
RefSeqiXP_961022.1. XM_955929.2.
UniGeneiNcr.25087.

Genome annotation databases

EnsemblFungiiEFNCRT00000004401; EFNCRP00000004401; EFNCRG00000004395.
GeneIDi3877201.
KEGGincr:NCU01115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL353820 Genomic DNA. Translation: CAB88602.1 .
CM002240 Genomic DNA. Translation: EAA31786.1 .
PIRi T48786.
RefSeqi XP_961022.1. XM_955929.2.
UniGenei Ncr.25087.

3D structure databases

ProteinModelPortali Q9P6Y2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 5141.NCU01115.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000004401 ; EFNCRP00000004401 ; EFNCRG00000004395 .
GeneIDi 3877201.
KEGGi ncr:NCU01115.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000159660.
KOi K15322.
OMAi WLHCVNR.
OrthoDBi EOG7SXWFH.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiSEN2_NEUCR
AccessioniPrimary (citable) accession number: Q9P6Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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