Q9P6Y2 (SEN2_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA-splicing endonuclease subunit sen-2
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease sen-2

Gene names

Name:	sen-2
ORF Names:	13E11.340, NCU01115

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of sen-2, sen-15, sen-34 and sen-54. Interacts directly with sen-54 By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	Probable tRNA-splicing endonuclease subunit sen-2		PRO_0000109460
Sites
	Active site	535	1	By similarity
	Active site	543	1	By similarity
	Active site	586	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P6Y2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F7536C1E5E62563E	FASTA	633	70,104
	10 20 30 40 50 60 MAENMTIATM AQPSESPSKS GEEAPASTNP AATSAPPRPP RVPYHQIYKL PAPIRTFPLP 70 80 90 100 110 120 TFYPSNPLSL FHLAVAWLRQ VLLPPPAEPS VIHEGIWDPD TRSVHVKDPK SIRALWEQGF 130 140 150 160 170 180 YGKGSLSRSE PNWFKRELSR RGLDGTTVSE ERTASRREER RQVKWERAKA ELEAIEKQKH 190 200 210 220 230 240 EEAKLNSETV NAAIPEPPAA EIEPEPEVLL SSLPDHVLSA TSTIPLSRAA PTNEFVPKTV 250 260 270 280 290 300 AHGHVEPKPP VGPLELLALP NSYALVRGEE LALSQPEPAP TEENVRKELK APVGPIELLA 310 320 330 340 350 360 LPNSLVDLVA LSVVSVLSED AGVDEDESST EDQLEASALT NDEPETSATE ANDSTSSSSP 370 380 390 400 410 420 PHTDGLPDSQ PTQENVPATP ERRKSVRFSS TVESTTFQHT DAASEVKNNN GSSDVEVFSS 430 440 450 460 470 480 FVSAVEKPVI LNVEPTVISQ ELVDKEHFQL APEEAFFLTF GLGALRVVDP VTEAPISNEQ 490 500 510 520 530 540 LLIKLRANSY FPPRSVDNLS PEDPFLVQYA VYHHFRSLGW VPRHGIKFGV DWIIYQRGPV 550 560 570 580 590 600 FDHSEFGIMV VPSFSDPRWS EFEHEESKKT WSWLMGVNRV LSHVLKSLVL VYVDVPPPPV 610 620 630 FDEEMKKGGI AAALKKFTIR EVMVRRFSVN RNR

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL353820 Genomic DNA. Translation: CAB88602.1. AABX02000015 Genomic DNA. Translation: EAA31786.1.
PIR	T48786.
RefSeq	XP_961022.1. XM_955929.2.
UniGene	Ncr.25087.
3D structure databases
ProteinModelPortal	Q9P6Y2.
ModBase	Search...
Protein-protein interaction databases
STRING	5141.NCU01115.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000004401; EFNCRP00000004401; EFNCRG00000004395.
GeneID	3877201.
KEGG	ncr:NCU01115.
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000159660.
KO	K15322.
OMA	PAPIRTF.
OrthoDB	EOG4PCF2K.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. [Graphical view]
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SEN2_NEUCR

Accession

Primary (citable) accession number: Q9P6Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program