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Protein

Probable tRNA-splicing endonuclease subunit tsp-2

Gene

tsp-2

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei535By similarity1
Active sitei543By similarity1
Active sitei586By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA-splicing endonuclease subunit tsp-2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease sen2
tRNA-splicing protein 2
Gene namesi
Name:tsp-2
Synonyms:sen2
ORF Names:13E11.340, NCU01115
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 2, Linkage Group V

Organism-specific databases

EuPathDBiFungiDB:NCU01115

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094601 – 633Probable tRNA-splicing endonuclease subunit tsp-2Add BLAST633

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-5/sen54 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9P6Y2
SMRiQ9P6Y2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

HOGENOMiHOG000159660
InParanoidiQ9P6Y2
KOiK15322
OMAiWLHCVNR
OrthoDBiEOG092C2YVE

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

Q9P6Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENMTIATM AQPSESPSKS GEEAPASTNP AATSAPPRPP RVPYHQIYKL
60 70 80 90 100
PAPIRTFPLP TFYPSNPLSL FHLAVAWLRQ VLLPPPAEPS VIHEGIWDPD
110 120 130 140 150
TRSVHVKDPK SIRALWEQGF YGKGSLSRSE PNWFKRELSR RGLDGTTVSE
160 170 180 190 200
ERTASRREER RQVKWERAKA ELEAIEKQKH EEAKLNSETV NAAIPEPPAA
210 220 230 240 250
EIEPEPEVLL SSLPDHVLSA TSTIPLSRAA PTNEFVPKTV AHGHVEPKPP
260 270 280 290 300
VGPLELLALP NSYALVRGEE LALSQPEPAP TEENVRKELK APVGPIELLA
310 320 330 340 350
LPNSLVDLVA LSVVSVLSED AGVDEDESST EDQLEASALT NDEPETSATE
360 370 380 390 400
ANDSTSSSSP PHTDGLPDSQ PTQENVPATP ERRKSVRFSS TVESTTFQHT
410 420 430 440 450
DAASEVKNNN GSSDVEVFSS FVSAVEKPVI LNVEPTVISQ ELVDKEHFQL
460 470 480 490 500
APEEAFFLTF GLGALRVVDP VTEAPISNEQ LLIKLRANSY FPPRSVDNLS
510 520 530 540 550
PEDPFLVQYA VYHHFRSLGW VPRHGIKFGV DWIIYQRGPV FDHSEFGIMV
560 570 580 590 600
VPSFSDPRWS EFEHEESKKT WSWLMGVNRV LSHVLKSLVL VYVDVPPPPV
610 620 630
FDEEMKKGGI AAALKKFTIR EVMVRRFSVN RNR
Length:633
Mass (Da):70,104
Last modified:October 1, 2000 - v1
Checksum:iF7536C1E5E62563E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353820 Genomic DNA Translation: CAB88602.1
CM002240 Genomic DNA Translation: EAA31786.1
PIRiT48786
RefSeqiXP_961022.1, XM_955929.3

Genome annotation databases

EnsemblFungiiEAA31786; EAA31786; NCU01115
GeneIDi3877201
KEGGincr:NCU01115

Similar proteinsi

Entry informationi

Entry nameiSEN2_NEUCR
AccessioniPrimary (citable) accession number: Q9P6Y2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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