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Q9P6Y2

- SEN2_NEUCR

UniProt

Q9P6Y2 - SEN2_NEUCR

Protein

Probable tRNA-splicing endonuclease subunit tsp-2

Gene

tsp-2

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. This subunit may anchor the endonuclease complex to the nuclear membrane. Probably carries the active site for 5'-splice site cleavage By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei535 – 5351By similarity
    Active sitei543 – 5431By similarity
    Active sitei586 – 5861By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA-splicing endonuclease subunit tsp-2 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease sen2
    tRNA-splicing protein 2
    Gene namesi
    Name:tsp-2
    Synonyms:sen2
    ORF Names:13E11.340, NCU01115
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 633633Probable tRNA-splicing endonuclease subunit tsp-2PRO_0000109460Add
    BLAST

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of tsp-2/sen2, tsp-1/sen15, tsp-4/sen34 and tsp-5/sen54. Interacts directly with tsp-5/sen54 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi5141.NCU01115.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P6Y2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000159660.
    KOiK15322.
    OMAiWLHCVNR.
    OrthoDBiEOG7SXWFH.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9P6Y2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAENMTIATM AQPSESPSKS GEEAPASTNP AATSAPPRPP RVPYHQIYKL    50
    PAPIRTFPLP TFYPSNPLSL FHLAVAWLRQ VLLPPPAEPS VIHEGIWDPD 100
    TRSVHVKDPK SIRALWEQGF YGKGSLSRSE PNWFKRELSR RGLDGTTVSE 150
    ERTASRREER RQVKWERAKA ELEAIEKQKH EEAKLNSETV NAAIPEPPAA 200
    EIEPEPEVLL SSLPDHVLSA TSTIPLSRAA PTNEFVPKTV AHGHVEPKPP 250
    VGPLELLALP NSYALVRGEE LALSQPEPAP TEENVRKELK APVGPIELLA 300
    LPNSLVDLVA LSVVSVLSED AGVDEDESST EDQLEASALT NDEPETSATE 350
    ANDSTSSSSP PHTDGLPDSQ PTQENVPATP ERRKSVRFSS TVESTTFQHT 400
    DAASEVKNNN GSSDVEVFSS FVSAVEKPVI LNVEPTVISQ ELVDKEHFQL 450
    APEEAFFLTF GLGALRVVDP VTEAPISNEQ LLIKLRANSY FPPRSVDNLS 500
    PEDPFLVQYA VYHHFRSLGW VPRHGIKFGV DWIIYQRGPV FDHSEFGIMV 550
    VPSFSDPRWS EFEHEESKKT WSWLMGVNRV LSHVLKSLVL VYVDVPPPPV 600
    FDEEMKKGGI AAALKKFTIR EVMVRRFSVN RNR 633
    Length:633
    Mass (Da):70,104
    Last modified:October 1, 2000 - v1
    Checksum:iF7536C1E5E62563E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353820 Genomic DNA. Translation: CAB88602.1.
    CM002240 Genomic DNA. Translation: EAA31786.1.
    PIRiT48786.
    RefSeqiXP_961022.1. XM_955929.2.
    UniGeneiNcr.25087.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000004401; EFNCRP00000004401; EFNCRG00000004395.
    GeneIDi3877201.
    KEGGincr:NCU01115.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353820 Genomic DNA. Translation: CAB88602.1 .
    CM002240 Genomic DNA. Translation: EAA31786.1 .
    PIRi T48786.
    RefSeqi XP_961022.1. XM_955929.2.
    UniGenei Ncr.25087.

    3D structure databases

    ProteinModelPortali Q9P6Y2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU01115.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000004401 ; EFNCRP00000004401 ; EFNCRG00000004395 .
    GeneIDi 3877201.
    KEGGi ncr:NCU01115.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000159660.
    KOi K15322.
    OMAi WLHCVNR.
    OrthoDBi EOG7SXWFH.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiSEN2_NEUCR
    AccessioniPrimary (citable) accession number: Q9P6Y2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3