Reviewed, UniProtKB/Swiss-Prot Q9P6R5 (GPI14_SCHPO)

Names and origin

Protein names

Recommended name:
    GPI mannosyltransferase 1
    EC=2.4.1.-
Alternative name(s):
    GPI mannosyltransferase I
      Short name=GPI-MT-I
    Glycosylphosphatidylinositol-anchor biosynthesis protein 14

Gene names

Name:	gpi14
ORF Names:	SPBC13E7.05

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity By similarity.
Pathway	Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the PIGM family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation GPI-anchor biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GPI anchor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid mannosylation Inferred by curator. Source: GeneDB_SPombe
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring hexosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	GPI mannosyltransferase 1		PRO_0000246233
Regions
	Transmembrane	64 – 84	21	Potential
	Transmembrane	120 – 140	21	Potential
	Transmembrane	144 – 164	21	Potential
	Transmembrane	190 – 210	21	Potential
	Transmembrane	248 – 268	21	Potential
	Transmembrane	290 – 310	21	Potential
	Transmembrane	318 – 338	21	Potential
	Transmembrane	344 – 364	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9P6R5-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 96B68CC3452E0245	FASTA	371	43,105
	10 20 30 40 50 60 MRLVLLNYGI WHDRRSALKF TDIDYFVFSD ASKYVSIGMS PYMRDTYRYT PMLAILLLPT 70 80 90 100 110 120 QYGFPSWGKY LFSISDLIAG WLMIKLLSRR ISYKRSLIYS SFWILNPFVA IISTRGNCEA 130 140 150 160 170 180 ILGILSIALL YLIEKKSVWL ASLILGFSVH FKIYPFMYGI AFLVYFSKPK KGSTFMEKFL 190 200 210 220 230 240 SLLSINQLKI VVGSLFMFTI CNLLMYYLYG SPFLEHTYLY HFGRTDHRHN FSLHHLNLYY 250 260 270 280 290 300 ESSFGAKASS LFAFLPQLSL CMLIPLVFGK KNLPGTLFAQ TFAFVTFNKV CTSQYFMWYL 310 320 330 340 350 360 VFLPLVLPNS KLLSKKGLIC LSLWIIGQLL WLISAYNLEM LGKSVFIPLW LSGLLFFFFN 370 VYELKIILDS L

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB89880.1. Different initiation.
RefSeq	NP_596260.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT50. Glycosyltransferase Family 50.
Genome annotation databases
GeneID	2540126.
KEGG	spo:SPBC13E7.05.
Organism-specific databases
GeneDB_Spombe	SPBC13E7.05.
Gene expression databases
ArrayExpress	Q9P6R5.
Family and domain databases
InterPro	IPR007704. Mannosyltransferase_DXD. [Graphical view]
PANTHER	PTHR12886. Mannos_trans_DXD. 1 hit.
Pfam	PF05007. Mannosyl_trans. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPI14_SCHPO

Accession

Primary (citable) accession number: Q9P6R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)