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Protein

Transcription factor IIIB 60 kDa subunit

Gene

brf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3ZincBy similarity1
Metal bindingi6ZincBy similarity1
Metal bindingi22ZincBy similarity1
Metal bindingi25ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 30TFIIB-typePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase III type 3 promoter sequence-specific DNA binding Source: PomBase
  • TBP-class protein binding Source: InterPro
  • TFIIIB-type transcription factor activity Source: PomBase

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 60 kDa subunit
Short name:
TFIIIB
Alternative name(s):
B-related factor 1
Short name:
BRF-1
TFIIB-related factor
Gene namesi
Name:brf1
ORF Names:SPBC13E7.10c, SPBC30D10.20
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC13E7.10c
PomBaseiSPBC13E7.10c brf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193451 – 492Transcription factor IIIB 60 kDa subunitAdd BLAST492

Proteomic databases

MaxQBiQ9P6R0
PaxDbiQ9P6R0
PRIDEiQ9P6R0

PTM databases

iPTMnetiQ9P6R0

Interactioni

Subunit structurei

TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and a third subunit (Potential). Interacts with maf1.Curated2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi276609, 8 interactors
STRINGi4896.SPBC13E7.10c.1

Structurei

3D structure databases

SMRiQ9P6R0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 30TFIIB-typePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000198688
InParanoidiQ9P6R0
KOiK15196
OMAiHDPPSFY
OrthoDBiEOG092C14D8

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR029529 Brf1
IPR011665 BRF1_TBP-bd_dom
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR000812 TFIIB
IPR013150 TFIIB_cyclin
IPR013137 Znf_TFIIB
PANTHERiPTHR11618 PTHR11618, 1 hit
PTHR11618:SF4 PTHR11618:SF4, 1 hit
PfamiView protein in Pfam
PF07741 BRF1, 1 hit
PF08271 TF_Zn_Ribbon, 1 hit
PF00382 TFIIB, 2 hits
PRINTSiPR00685 TIFACTORIIB
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS51134 ZF_TFIIB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9P6R0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCPNCGSTT FESDTASGNT YCTQCGVVVE QDAIVSEVTF GEASTGAAVV
60 70 80 90 100
QGSLVSNDQT HARTFGGPYR NQGSVESREL TIANGRRRIS ALAIALKLNE
110 120 130 140 150
RHIEAAVRYF TLAINNNFIK GRRSQYVVAS CLYIVCRISK TSHMLIDFSD
160 170 180 190 200
ILQINVFKLG STFLKLCRVL RPNLPLLDPS LYISRFASLL EFGPETHRVA
210 220 230 240 250
NDAIRLVARM NRDWMQIGRR PAGICGACLL IAARMNNFRR SVREVVHVVK
260 270 280 290 300
VADITIQKRL DEFKLTESGD LSIADFRNIW LEGQSDPPSF TKNQKFQQYG
310 320 330 340 350
AQKVSNIDHT QEYMSPIKRT PDFDGNEVKS EELSQTVKVE SQETPVHLKA
360 370 380 390 400
DEREIRKEVT ETLKGDELRK ISLQVNVKFS EEEVTLEDVD DDEIEDILLD
410 420 430 440 450
KDEILTKTQV WMELNKDYLA EEEAKNLKLQ EDLKKGIVRQ PRKRRRYRPR
460 470 480 490
DSTSDGIADT AAESAKEMMQ QRAFSKKINY EALDMLFDEE QS
Length:492
Mass (Da):55,795
Last modified:July 11, 2012 - v2
Checksum:i34B1874ECBB05D3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAB89885.2
PIRiT40176
RefSeqiNP_596265.2, NM_001022185.2

Genome annotation databases

EnsemblFungiiSPBC13E7.10c.1; SPBC13E7.10c.1:pep; SPBC13E7.10c
KEGGispo:SPBC13E7.10c

Similar proteinsi

Entry informationi

Entry nameiTF3B_SCHPO
AccessioniPrimary (citable) accession number: Q9P6R0
Secondary accession number(s): Q9USU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 11, 2012
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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