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Protein

Transcription factor IIIB 60 kDa subunit

Gene

brf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi3ZincBy similarity1
Metal bindingi6ZincBy similarity1
Metal bindingi22ZincBy similarity1
Metal bindingi25ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 30TFIIB-typePROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 60 kDa subunit
Short name:
TFIIIB
Alternative name(s):
B-related factor 1
Short name:
BRF-1
TFIIB-related factor
Gene namesi
Name:brf1
ORF Names:SPBC13E7.10c, SPBC30D10.20
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC13E7.10c.
PomBaseiSPBC13E7.10c. brf1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • transcription factor TFIIIB complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193451 – 492Transcription factor IIIB 60 kDa subunitAdd BLAST492

Proteomic databases

MaxQBiQ9P6R0.
PRIDEiQ9P6R0.

Interactioni

Subunit structurei

TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and a third subunit (Potential). Interacts with maf1.Curated2 Publications

Protein-protein interaction databases

BioGridi276609. 4 interactors.
MINTiMINT-4704616.

Family & Domainsi

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1 – 30TFIIB-typePROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000198688.
InParanoidiQ9P6R0.
KOiK15196.
OMAiISMAHRY.
OrthoDBiEOG092C14D8.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PTHR11618:SF4. PTHR11618:SF4. 1 hit.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P6R0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCPNCGSTT FESDTASGNT YCTQCGVVVE QDAIVSEVTF GEASTGAAVV
60 70 80 90 100
QGSLVSNDQT HARTFGGPYR NQGSVESREL TIANGRRRIS ALAIALKLNE
110 120 130 140 150
RHIEAAVRYF TLAINNNFIK GRRSQYVVAS CLYIVCRISK TSHMLIDFSD
160 170 180 190 200
ILQINVFKLG STFLKLCRVL RPNLPLLDPS LYISRFASLL EFGPETHRVA
210 220 230 240 250
NDAIRLVARM NRDWMQIGRR PAGICGACLL IAARMNNFRR SVREVVHVVK
260 270 280 290 300
VADITIQKRL DEFKLTESGD LSIADFRNIW LEGQSDPPSF TKNQKFQQYG
310 320 330 340 350
AQKVSNIDHT QEYMSPIKRT PDFDGNEVKS EELSQTVKVE SQETPVHLKA
360 370 380 390 400
DEREIRKEVT ETLKGDELRK ISLQVNVKFS EEEVTLEDVD DDEIEDILLD
410 420 430 440 450
KDEILTKTQV WMELNKDYLA EEEAKNLKLQ EDLKKGIVRQ PRKRRRYRPR
460 470 480 490
DSTSDGIADT AAESAKEMMQ QRAFSKKINY EALDMLFDEE QS
Length:492
Mass (Da):55,795
Last modified:July 11, 2012 - v2
Checksum:i34B1874ECBB05D3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB89885.2.
PIRiT40176.
RefSeqiNP_596265.2. NM_001022185.2.

Genome annotation databases

EnsemblFungiiSPBC13E7.10c.1; SPBC13E7.10c.1:pep; SPBC13E7.10c.
GeneIDi2540071.
KEGGispo:SPBC13E7.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA. Translation: CAB89885.2.
PIRiT40176.
RefSeqiNP_596265.2. NM_001022185.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276609. 4 interactors.
MINTiMINT-4704616.

Proteomic databases

MaxQBiQ9P6R0.
PRIDEiQ9P6R0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC13E7.10c.1; SPBC13E7.10c.1:pep; SPBC13E7.10c.
GeneIDi2540071.
KEGGispo:SPBC13E7.10c.

Organism-specific databases

EuPathDBiFungiDB:SPBC13E7.10c.
PomBaseiSPBC13E7.10c. brf1.

Phylogenomic databases

HOGENOMiHOG000198688.
InParanoidiQ9P6R0.
KOiK15196.
OMAiISMAHRY.
OrthoDBiEOG092C14D8.

Miscellaneous databases

PROiQ9P6R0.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR029529. Brf1.
IPR011665. BRF1_TBP-bd_dom.
IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PTHR11618:SF4. PTHR11618:SF4. 1 hit.
PfamiPF07741. BRF1. 1 hit.
PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF3B_SCHPO
AccessioniPrimary (citable) accession number: Q9P6R0
Secondary accession number(s): Q9USU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: July 11, 2012
Last modified: November 30, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.