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Protein

Kinetochore protein mis14

Gene

mis14

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the NMS (Ndc80-MIND-Spc7) super complex which has a role in kinetochore function during late meiotic prophase and throughout the mitotic cell cycle. Required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialized chromatin at the centromere.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic sister chromatid segregation Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein mis14
Alternative name(s):
NMS complex subunit mis14
Gene namesi
Name:mis14
ORF Names:SPAC688.02c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC688.02c.
PomBaseiSPAC688.02c. mis14.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome, centromeric region Source: PomBase
  • condensed nuclear chromosome inner kinetochore Source: PomBase
  • condensed nuclear chromosome kinetochore Source: PomBase
  • cytosol Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • NMS complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Kinetochore protein mis14PRO_0000278390Add
BLAST

Proteomic databases

MaxQBiQ9P6M3.

Interactioni

Subunit structurei

Component of the NMS super complex which consists of mis12, mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7, spc24 and spc25. Interacts with mis12.3 Publications

Protein-protein interaction databases

BioGridi279902. 7 interactions.
IntActiQ9P6M3. 1 interaction.
MINTiMINT-4704341.

Family & Domainsi

Phylogenomic databases

InParanoidiQ9P6M3.
KOiK11561.
OMAiRESTEHI.
OrthoDBiEOG790GC2.
PhylomeDBiQ9P6M3.

Family and domain databases

InterProiIPR013950. Kinetochore_Mis14.
[Graphical view]
PfamiPF08641. Mis14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P6M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSHGDASVL PKIALESIND YSYVKSVFRQ AIQEKLAIHL PEQASREAGM
60 70 80 90 100
GREDQLRIRV KEMLNELIES TFSIIQENVT INGFDANSAL QDPKNSDQIE
110 120 130 140 150
PFDLALRTRV QQLFNEVEDA HVLVARYRKS VPAQYEKAYV DAMEQQTAFL
160 170 180 190 200
RNVKDDYVSL QDKEVENPDE QTSTTVFRKE DIDRYEQTIA KVAYLKKNLP
210
RVVARLEKTP
Length:210
Mass (Da):24,122
Last modified:October 1, 2000 - v1
Checksum:i400DC20681812390
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB90769.1.
RefSeqiNP_594061.1. NM_001019485.2.

Genome annotation databases

EnsemblFungiiSPAC688.02c.1; SPAC688.02c.1:pep; SPAC688.02c.
GeneIDi2543482.
KEGGispo:SPAC688.02c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB90769.1.
RefSeqiNP_594061.1. NM_001019485.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279902. 7 interactions.
IntActiQ9P6M3. 1 interaction.
MINTiMINT-4704341.

Proteomic databases

MaxQBiQ9P6M3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC688.02c.1; SPAC688.02c.1:pep; SPAC688.02c.
GeneIDi2543482.
KEGGispo:SPAC688.02c.

Organism-specific databases

EuPathDBiFungiDB:SPAC688.02c.
PomBaseiSPAC688.02c. mis14.

Phylogenomic databases

InParanoidiQ9P6M3.
KOiK11561.
OMAiRESTEHI.
OrthoDBiEOG790GC2.
PhylomeDBiQ9P6M3.

Miscellaneous databases

NextBioi20804494.
PROiQ9P6M3.

Family and domain databases

InterProiIPR013950. Kinetochore_Mis14.
[Graphical view]
PfamiPF08641. Mis14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres."
    Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M.
    Cell 118:715-729(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MIS12, SUBCELLULAR LOCATION.
  3. "Molecular analysis of kinetochore architecture in fission yeast."
    Liu X., McLeod I., Anderson S., Yates J.R. III, He X.
    EMBO J. 24:2919-2930(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NMS COMPLEX.
  4. "Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe."
    Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.
    Mol. Biol. Cell 17:5173-5184(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NMS COMPLEX.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMIS14_SCHPO
AccessioniPrimary (citable) accession number: Q9P6M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.