Q9P6M1 (GST3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 3
EC=2.5.1.18

Alternative name(s):
GST-III

Gene names

Name:	gst3
ORF Names:	SPAC688.04c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a role in the detoxification of various heavy metals. Ref.1
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Interacts with sad1. Ref.4
Subcellular location	Cytoplasm Ref.4.
Induction	By heavy metals such as cadmium and mercury. Ref.1
Sequence similarities	Belongs to the GST superfamily. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	removal of superoxide radicals Inferred by curator. Source: PomBase response to drug Inferred from sequence or structural similarity PubMed 12063243. Source: PomBase
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	glutathione peroxidase activity Inferred from direct assay PubMed 12063243. Source: PomBase glutathione transferase activity Inferred from direct assay Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Glutathione S-transferase 3		PRO_0000185986
Regions
	Domain	1 – 79	79	GST N-terminal
	Domain	85 – 234	150	GST C-terminal
Amino acid modifications
	Modified residue	228	1	Phosphoserine Ref.5
	Modified residue	232	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9P6M1 [UniParc]. Last modified April 18, 2012. Version 3. Checksum: 42324A6B9B672938	FASTA	242	28,113
	10 20 30 40 50 60 MIVLHHLKNS RSTRIVWMLE ELKVPYEIKV YDRVDGRAPP AYTKLSPLGK SPIVVDDGVT 70 80 90 100 110 120 YIESAAILEH LVRKYGPSFK PSEEDVAELE KYELWMHFSE ASLMPFIWAS HVLDLSVNMT 130 140 150 160 170 180 PIFFRYIVRQ FVNGIKSKYL SKETFLNLDY IDNHLASNEY FAGEQFTAAD PQMCFPIFAA 190 200 210 220 230 240 QRDYLSQKPY KNIKRWMRVV SDRPACRIAA EKVEDNTLTL FSDVERYSHP PTPPPEQVRS DE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization, expression and regulation of a third gene encoding glutathione S-transferase from the fission yeast." Shin Y.H., Park E.-H., Fuchs J.A., Lim C.-J. Biochim. Biophys. Acta 1577:164-170(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"Comparative functional genomics of the fission yeasts." Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K. , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C. Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVISION OF GENE MODEL.
[4]	"Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast." Miki F., Kurabayashi A., Tange Y., Okazaki K., Shimanuki M., Niwa O. Mol. Genet. Genomics 270:449-461(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SAD1, SUBCELLULAR LOCATION.
[5]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228 AND THR-232, MASS SPECTROMETRY.
[6]	"Augmented annotation of the Schizosaccharomyces pombe genome reveals additional genes required for growth and viability." Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L., Hagan I.M., Miller C.J. Genetics 187:1207-1217(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVISION OF GENE MODEL, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY034792 Genomic DNA. Translation: AAK58430.1. CU329670 Genomic DNA. Translation: CAB90771.3.
RefSeq	NP_594063.3. NM_001019487.3.
3D structure databases
ProteinModelPortal	Q9P6M1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P6M1. 1 interaction.
STRING	4896.SPAC688.04c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC688.04c.1; SPAC688.04c.1:pep; SPAC688.04c.
GeneID	2543479.
KEGG	spo:SPAC688.04c.
Organism-specific databases
PomBase	SPAC688.04c.
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125753.
OrthoDB	EOG4GMZ6F.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804491.

Entry information

Entry name

GST3_SCHPO

Accession

Primary (citable) accession number: Q9P6M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	April 18, 2012
Last modified:	May 1, 2013
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program