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Protein

DNA polymerase zeta catalytic subunit

Gene

rev3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Has a role in the bypass of abasic (AP) sites (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi1381 – 13811ZincBy similarity
Metal bindingi1384 – 13841ZincBy similarity
Metal bindingi1400 – 14001ZincBy similarity
Metal bindingi1403 – 14031ZincBy similarity
Metal bindingi1432 – 14321Iron-sulfur (4Fe-4S)By similarity
Metal bindingi1435 – 14351Iron-sulfur (4Fe-4S)By similarity
Metal bindingi1446 – 14461Iron-sulfur (4Fe-4S)By similarity
Metal bindingi1451 – 14511Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1381 – 140323CysA-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: PomBase
  • DNA replication Source: UniProtKB-KW
  • error-free translesion synthesis Source: PomBase
  • error-prone translesion synthesis Source: PomBase
  • mitochondrial DNA repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase zeta catalytic subunit (EC:2.7.7.7)
Alternative name(s):
Protein reversionless 3
Gene namesi
Name:rev3
ORF Names:SPAC688.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC688.10.
PomBaseiSPAC688.10. rev3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
  • zeta DNA polymerase complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14801480DNA polymerase zeta catalytic subunitPRO_0000361055Add
BLAST

Interactioni

Subunit structurei

Forms DNA polymerase zeta with rev7.By similarity

Protein-protein interaction databases

BioGridi279780. 25 interactions.
MINTiMINT-4704273.

Structurei

3D structure databases

ProteinModelPortaliQ9P6L6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1432 – 145120CysB motifAdd
BLAST

Domaini

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Sequence analysis
Contains 1 CysA-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1381 – 140323CysA-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000194392.
InParanoidiQ9P6L6.
KOiK02350.
OMAiYETHASS.
OrthoDBiEOG092C0C07.
PhylomeDBiQ9P6L6.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR030559. PolZ_Rev3.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view]
PANTHERiPTHR10322:SF5. PTHR10322:SF5. 2 hits.
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P6L6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFSIEYIDW ELSPCDPAYD FVGKDLPTAN EELTTVPVIR VFGLNEEAET
60 70 80 90 100
VCCFIHNVFP YIYVEYSSFA ETLDLEVPDF LSQLQTSINY ALALAARANP
110 120 130 140 150
ETYKPAVQSV QLVKGIPFYG YSFCFQKFLK ICLFSPKNRD RLVDLFRQGA
160 170 180 190 200
ILNKVIQVYE SHLPYLLQFM VDHNLYGCAP IDLDDSIIKR DDLLSFCNVE
210 220 230 240 250
VHVSPNAILN ACWLSERNIH TDLYETHASS PNSLLVTSLA EIWKSEASRR
260 270 280 290 300
NLTSSDETNS FSKLHQSQFG LKEESSHEPR SSQHWKNEVA MKDLLKNLIK
310 320 330 340 350
SKLESSSDVN TPLIFDPWPE LPTIYSAIHT KDYVRPSQND ISVSQISVDE
360 370 380 390 400
KICTSYESLP KIQLEQNTAP VIESSFEQLD SELERILGDT LFDSFPYVEP
410 420 430 440 450
NVDRSKFPKS PLNSSQEVTI HSSQDRQSPP SSPLKDVPSQ INPFSPSLRL
460 470 480 490 500
KGGSPITKRE IEFCRDLPNR PTSSEPNQGD TRKAGKRLKY SRNLDDYHIC
510 520 530 540 550
TQIPEDYSPK FLSQHESFVY KQQPPSTDDL YGTMKKLKIP FSIPTNVHYS
560 570 580 590 600
SEKDIPSYSQ EYLGKSHYPI GVSSRYLPEF QSDGSVSEKV RLNPLKLSNF
610 620 630 640 650
HGERTWQYIK PAPLAVDLSN LESKEAVSEE IQSPQRLSRS KVFRKDPYSC
660 670 680 690 700
VRILALELFC CSHGGLTPDP TKDSIECCFW AYQEDVNSSM IDRVGFIVVD
710 720 730 740 750
KSASNSSFGR SFPSCTVLVV NSELELINEV IGLNRQLDPT IVCGYEVHNS
760 770 780 790 800
SWGYLIERAS YRFNYDLPEQ LSRLKCTSKA NFAKKENAWK YTTTSSINIV
810 820 830 840 850
GRHVLNIWRI LRGEVNLLNY SLENVVLNIF KKQTPYYNQA DKVHLWQSSR
860 870 880 890 900
FHEKQILLNY MLNRTRYCLE ILSACAIVTK IREQARIIGI DFMSVISRGS
910 920 930 940 950
QFKVESIMFR IAKPENYIFP SPSAKQVAEQ NALEALPLVM EPKSDLYNNP
960 970 980 990 1000
VVVLDFQSLY PSIIIAYNLC YSTCLGPVKI VNGKVKLGFM FHSSNPNIVN
1010 1020 1030 1040 1050
LIKNDVYISP NGYAYVKENV RKSLLAKMLE ELIETRNMVK RGMKDCDSDY
1060 1070 1080 1090 1100
VNKVLNSRQL ALKLIANVTY GYTSASFSGR MPCSEIADTI VETGREILSY
1110 1120 1130 1140 1150
SLEYINTLDF CHAKVVYGDT DSLFVELPGA TKEQAFDIGQ QLANNITSRF
1160 1170 1180 1190 1200
PSPIRLKFEK IYFPCFLLAK KRYVGFKFES VSQKAPIFEA KGIETVRRDG
1210 1220 1230 1240 1250
TPVQQQLLRR CLEILFKTKD LSTVKKEFQN VCYQIMSGNV PVMDFCFSKE
1260 1270 1280 1290 1300
VRLEKYKELS TAPPGAVMAR RLMTKDPRRE PQYGERVPYL IIAAAPGTTL
1310 1320 1330 1340 1350
ANRSVAPEEF LSSSFSQLDI NYYINNSLIP PLDRFLNLLG ASAQSWYHEM
1360 1370 1380 1390 1400
PKPRTSLKLT ETVKGGIQKK TLDTFLMEKL CSSCLKNNIE IIPDKINSLC
1410 1420 1430 1440 1450
SDCLKNPCAT ISKAVTQHNA YNKKLSLLFD ICRGCSKLSS SDPVLCKSNS
1460 1470 1480
CKVYYDRAKT ENYAKVQAEM LTKTLGSLDW
Length:1,480
Mass (Da):168,290
Last modified:October 1, 2000 - v1
Checksum:i0490869066988AE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB90776.1.
RefSeqiNP_594068.1. NM_001019492.2.

Genome annotation databases

EnsemblFungiiSPAC688.10.1; SPAC688.10.1:pep; SPAC688.10.
GeneIDi2543358.
KEGGispo:SPAC688.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB90776.1.
RefSeqiNP_594068.1. NM_001019492.2.

3D structure databases

ProteinModelPortaliQ9P6L6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279780. 25 interactions.
MINTiMINT-4704273.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC688.10.1; SPAC688.10.1:pep; SPAC688.10.
GeneIDi2543358.
KEGGispo:SPAC688.10.

Organism-specific databases

EuPathDBiFungiDB:SPAC688.10.
PomBaseiSPAC688.10. rev3.

Phylogenomic databases

HOGENOMiHOG000194392.
InParanoidiQ9P6L6.
KOiK02350.
OMAiYETHASS.
OrthoDBiEOG092C0C07.
PhylomeDBiQ9P6L6.

Enzyme and pathway databases

ReactomeiR-SPO-110312. Translesion synthesis by REV1.
R-SPO-5655862. Translesion synthesis by POLK.
R-SPO-5656121. Translesion synthesis by POLI.

Miscellaneous databases

PROiQ9P6L6.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.90.1600.10. 2 hits.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR023211. DNA_pol_palm_dom.
IPR030559. PolZ_Rev3.
IPR012337. RNaseH-like_dom.
IPR025687. Znf-C4pol.
[Graphical view]
PANTHERiPTHR10322:SF5. PTHR10322:SF5. 2 hits.
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14260. zf-C4pol. 1 hit.
[Graphical view]
PRINTSiPR00106. DNAPOLB.
SMARTiSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPOZ_SCHPO
AccessioniPrimary (citable) accession number: Q9P6L6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.