Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Deoxyhypusine hydroxylase

Gene

lia1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Iron 1UniRule annotation
Metal bindingi66 – 661Iron 1UniRule annotation
Metal bindingi98 – 981Iron 1UniRule annotation
Metal bindingi99 – 991Iron 1UniRule annotation
Metal bindingi227 – 2271Iron 2UniRule annotation
Metal bindingi228 – 2281Iron 2UniRule annotation
Metal bindingi260 – 2601Iron 2UniRule annotation
Metal bindingi261 – 2611Iron 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • mitochondrion distribution Source: PomBase
  • peptidyl-lysine modification to peptidyl-hypusine Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:lia1
Synonyms:mmd1
ORF Names:SPAC30C2.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC30C2.02.
PomBaseiSPAC30C2.02.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Deoxyhypusine hydroxylasePRO_0000283672Add
BLAST

Proteomic databases

MaxQBiQ9P6K9.

Interactioni

Protein-protein interaction databases

BioGridi277967. 7 interactions.
MINTiMINT-4704216.

Structurei

3D structure databases

ProteinModelPortaliQ9P6K9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati96 – 12227HEAT-like PBS-type 1Add
BLAST
Repeati194 – 22027HEAT-like PBS-type 2Add
BLAST
Repeati225 – 25127HEAT-like PBS-type 3Add
BLAST
Repeati258 – 28427HEAT-like PBS-type 4Add
BLAST

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 4 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000248665.
InParanoidiQ9P6K9.
KOiK06072.
OMAiPEFQYAD.
OrthoDBiEOG7QC85Z.
PhylomeDBiQ9P6K9.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P6K9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEPVPQAV IDELERVLVN LDKSNPLSFR YRALFSLNAL AKKGDKRAVD
60 70 80 90 100
AIYKAFIDDS ELLKHEMAYV MGQSGQQYAV QPLINIVNDL DQQVMVRHEA
110 120 130 140 150
AEALGALGFT ESLPVLEKYY KEDPLAPIRE TCELAIARIQ WKNGLDKNNE
160 170 180 190 200
KITPSMYDSV VDPAPPMPDH EQDVKSEVAK LRSEIVDQNL PLFYRYRVMF
210 220 230 240 250
RLRNIGNEEA VLALTDGFKD PSPLFRHEIA FVFGQMIAPA SVPALIKVLE
260 270 280 290 300
NTEEVPMVRH EAAEALGGIA NDECLPVLKK FSKDDVRVVA ESCIVALDMI
310
EYEKSGDMEY AYIPKVSA
Length:318
Mass (Da):35,689
Last modified:October 1, 2000 - v1
Checksum:i8A4BDE165C767282
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB90789.1.
RefSeqiNP_594654.1. NM_001020083.2.

Genome annotation databases

EnsemblFungiiSPAC30C2.02.1; SPAC30C2.02.1:pep; SPAC30C2.02.
GeneIDi2541465.
KEGGispo:SPAC30C2.02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB90789.1.
RefSeqiNP_594654.1. NM_001020083.2.

3D structure databases

ProteinModelPortaliQ9P6K9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277967. 7 interactions.
MINTiMINT-4704216.

Proteomic databases

MaxQBiQ9P6K9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC30C2.02.1; SPAC30C2.02.1:pep; SPAC30C2.02.
GeneIDi2541465.
KEGGispo:SPAC30C2.02.

Organism-specific databases

EuPathDBiFungiDB:SPAC30C2.02.
PomBaseiSPAC30C2.02.

Phylogenomic databases

HOGENOMiHOG000248665.
InParanoidiQ9P6K9.
KOiK06072.
OMAiPEFQYAD.
OrthoDBiEOG7QC85Z.
PhylomeDBiQ9P6K9.

Enzyme and pathway databases

UniPathwayiUPA00354.
ReactomeiR-SPO-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiQ9P6K9.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Mmd1p, a novel, conserved protein essential for normal mitochondrial morphology and distribution in the fission yeast Schizosaccharomyces pombe."
    Weir B.A., Yaffe M.P.
    Mol. Biol. Cell 15:1656-1665(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDOHH_SCHPO
AccessioniPrimary (citable) accession number: Q9P6K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.