Q9P6J3 (MALT_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-glucosidase
EC=3.2.1.20

Alternative name(s):
Maltase

Gene names

Name:	mal1
ORF Names:	SPBC1683.07

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Induction	Repressed by glucose and inositol.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular polysaccharide catabolic process Inferred by curator. Source: GeneDB_Spombe extracellular polysaccharide metabolic process Inferred by curator. Source: GeneDB_Spombe maltose catabolic process Inferred from direct assay. Source: GeneDB_Spombe
Cellular component	cell surface Inferred by curator. Source: GeneDB_Spombe cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from direct assay. Source: GeneDB_Spombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	Alpha-glucosidase		PRO_0000310319
Sites
	Active site	212	1	Nucleophile By similarity
	Active site	269	1	Proton donor By similarity
	Site	346	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	381	1	F → L in AAY57566. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9P6J3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7B79064326E445AA	FASTA	579	67,752
	10 20 30 40 50 60 MKVVPSEKIK PNWWRETSVY QIYPASFKDS NGDGFGDLEG IISKVDYLKA LNVESIWLCP 70 80 90 100 110 120 IYPSPLKDMG YDVSDYKQID SRYGTLEDLD RLMKALHERD MKLVMDLVLN HTSDQHEWFK 130 140 150 160 170 180 ESRSSKTNPK RDWYFWKPAR YNEKGERLPP NNWRSYFDTS AWEWDEATQE YYLHLWSVGQ 190 200 210 220 230 240 PDLNWETPKV REAVHDILRF WLDRGVDGFR LDAINMISKD QRFLDAPITD DRYEYQLAYQ 250 260 270 280 290 300 YYANGPRIHE YLNGIGNILT EYDAFSVGEM PYVLDTNEIL HVVGADRREL TMIFQFDFVD 310 320 330 340 350 360 LDLDPNQHKY IEGSWELSDL KKSLKKWQSA LLSGGGWNAS FIENHDQTRT VSRYLSDSPK 370 380 390 400 410 420 YRAYSSKLMA LFIIFQSGTP FVFQGQELAL ANIPRDWPID EYLDVETQNF WKLFMSGNPS 430 440 450 460 470 480 QEEIEKTMDI VNKRARDNGR TPMHWDSSPN GGFTKAGVKP WMRVTNDYKE WNAANQVNDP 490 500 510 520 530 540 ESPYTFWSKA LELRKELKDA VVYGSFELIS EEDPSIVAFV RESSTYKLII LLNFTGNKVS 550 560 570 YDCPLNLTSY EILLDNYKDF ICMTSPVTLN PYQAVLLKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of MAL1+ gene expression encoding maltase in Schizosaccharomyces pombe by added inositol." Yao S., Chi Z., He S. Indian J. Biochem. Biophys. 43:143-147(2006) [PubMed: 16967902] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], REPRESSION BY GLUCOSE AND INOSITOL.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ019991 Genomic DNA. Translation: AAY57566.1. CU329671 Genomic DNA. Translation: CAB91169.1.
RefSeq	NP_595063.1. NM_001020969.1.
3D structure databases
HSSP	HSSP built from PDB template 1UOK based on UniProtKB P21332.
ProteinModelPortal	Q9P6J3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9P6J3.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC1683.07.1; SPBC1683.07.1:pep; SPBC1683.07.
GeneID	2539976.
GenomeReviews	Gene locus mal1 in contig CU329671_GR.
KEGG	spo:SPBC1683.07.
NMPDR	fig|4896.1.peg.929.
Organism-specific databases
GeneDB_Spombe	SPBC1683.07.
Phylogenomic databases
eggNOG	fuNOG06455.
GeneTree	EFGT00050000000929.
HOGENOM	HBG669028.
OMA	ENRPETI.
OrthoDB	EOG4K0TWQ.
Gene expression databases
ArrayExpress	Q9P6J3.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.
KO	K01187.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MALT_SCHPO

Accession

Primary (citable) accession number: Q9P6J3
Secondary accession number(s): Q4U125

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program