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Protein

Adenine deaminase

Gene

aah1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N6-substituted adenines that are commonly known as the plant hormones cytokinins.UniRule annotation2 Publications

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Kineticsi

  1. KM=32 µM for adenine1 Publication

    pH dependencei

    Optimum pH is 6.7.1 Publication

    Temperature dependencei

    Optimum temperature is 33 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi19Zinc; catalyticUniRule annotation1
    Metal bindingi21Zinc; catalyticUniRule annotation1
    Metal bindingi209Zinc; catalyticUniRule annotation1
    Active sitei212Proton donorUniRule annotation1
    Sitei233Important for catalytic activityUniRule annotation1
    Metal bindingi290Zinc; catalyticUniRule annotation1
    Binding sitei291SubstrateUniRule annotation1

    GO - Molecular functioni

    • adenine deaminase activity Source: PomBase
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • adenine catabolic process Source: PomBase
    • hypoxanthine salvage Source: PomBase
    • nucleotide metabolic process Source: UniProtKB-KW
    • regulation of G2/M transition of mitotic cell cycle Source: PomBase

    Keywordsi

    Molecular functionHydrolase
    Biological processNucleotide metabolism
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.4.2 5613
    3.5.4.4 5613
    ReactomeiR-SPO-2161541 Abacavir metabolism
    R-SPO-74217 Purine salvage

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenine deaminaseUniRule annotation (EC:3.5.4.2UniRule annotation)
    Short name:
    ADEUniRule annotation
    Alternative name(s):
    Adenine aminohydrolaseUniRule annotation
    Short name:
    AAHUniRule annotation
    Gene namesi
    Name:aah1
    Synonyms:dea2
    ORF Names:SPBC1198.02
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:SPBC1198.02
    PomBaseiSPBC1198.02

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi150R → H in dea2-1: Abolishes adenine deaminase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002562371 – 367Adenine deaminaseAdd BLAST367

    Proteomic databases

    MaxQBiQ9P6I7
    PaxDbiQ9P6I7
    PRIDEiQ9P6I7

    Interactioni

    Protein-protein interaction databases

    BioGridi276604, 1 interactor
    STRINGi4896.SPBC1198.02.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P6I7
    SMRiQ9P6I7
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000218813
    KOiK01488
    OMAiQWCGADR
    OrthoDBiEOG092C2UZH
    PhylomeDBiQ9P6I7

    Family and domain databases

    CDDicd01320 ADA, 1 hit
    HAMAPiMF_01962 Adenine_deaminase, 1 hit
    InterProiView protein in InterPro
    IPR001365 A/AMP_deaminase_dom
    IPR028892 ADE
    IPR006330 Ado/ade_deaminase
    IPR032466 Metal_Hydrolase
    PfamiView protein in Pfam
    PF00962 A_deaminase, 1 hit
    SUPFAMiSSF51556 SSF51556, 1 hit
    TIGRFAMsiTIGR01430 aden_deam, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9P6I7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSNLPIYNFI RKLPKCEHHV HLEGCLSPDL VFRLAKKNGI TLPSDDAAYT
    60 70 80 90 100
    TPSTLLASYE HFGCLDDFLR YYYIAVSVLI EASDFEALAY EYFSIAHSQG
    110 120 130 140 150
    VHHAEVFFDP QTHTSRGISY DVVVSGFSAA CERANRDFGM STNLIMCFLR
    160 170 180 190 200
    HLPSEAAHET FAEALKRNDF ENGIVAGVGL DSSEVDFPPE LFQEVYKLAA
    210 220 230 240 250
    EKGIRRTGHA GEEGDPSYIR SGLDNLSLQR IDHGIRLVED KELMKRVAEE
    260 270 280 290 300
    NIMLTMCPLS NLKLRCVNSI AELPVREFLE AGVPFSINCD DPAYFGGYTL
    310 320 330 340 350
    ENYFAIQKHF NLTVKEWVFI ANAAINGSWI SGKRKEELLS SVQKCVKEYT
    360
    AEIQQPKTLE TAVEVQA
    Length:367
    Mass (Da):41,199
    Last modified:October 1, 2000 - v1
    Checksum:i4470A2EF23405B85
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329671 Genomic DNA Translation: CAB91177.1
    RefSeqiNP_595071.1, NM_001020977.2

    Genome annotation databases

    EnsemblFungiiSPBC1198.02.1; SPBC1198.02.1:pep; SPBC1198.02
    GeneIDi2540066
    KEGGispo:SPBC1198.02

    Similar proteinsi

    Entry informationi

    Entry nameiADE_SCHPO
    AccessioniPrimary (citable) accession number: Q9P6I7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: October 1, 2000
    Last modified: March 28, 2018
    This is version 104 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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