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Protein

Adenine deaminase

Gene

aah1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism. Also exhibits a low activity towards N(6)-substituted adenines that are commonly known as the plant hormones cytokinins.UniRule annotation2 Publications

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Kineticsi

  1. KM=32 µM for adenine1 Publication

    pH dependencei

    Optimum pH is 6.7.1 Publication

    Temperature dependencei

    Optimum temperature is 33 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi19 – 191Zinc; catalyticUniRule annotation
    Metal bindingi21 – 211Zinc; catalyticUniRule annotation
    Metal bindingi209 – 2091Zinc; catalyticUniRule annotation
    Active sitei212 – 2121Proton donorUniRule annotation
    Sitei233 – 2331Important for catalytic activityUniRule annotation
    Metal bindingi290 – 2901Zinc; catalyticUniRule annotation
    Binding sitei291 – 2911SubstrateUniRule annotation

    GO - Molecular functioni

    • adenine deaminase activity Source: PomBase
    • zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    • adenine catabolic process Source: PomBase
    • hypoxanthine salvage Source: PomBase
    • nucleotide metabolic process Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.5.4.2. 5613.
    3.5.4.4. 5613.
    ReactomeiR-SPO-2161541. Abacavir metabolism.
    R-SPO-74217. Purine salvage.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenine deaminaseUniRule annotation (EC:3.5.4.2UniRule annotation)
    Short name:
    ADEUniRule annotation
    Alternative name(s):
    Adenine aminohydrolaseUniRule annotation
    Short name:
    AAHUniRule annotation
    Gene namesi
    Name:aah1
    Synonyms:dea2
    ORF Names:SPBC1198.02
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    Proteomesi
    • UP000002485 Componenti: Chromosome II

    Organism-specific databases

    EuPathDBiFungiDB:SPBC1198.02.
    PomBaseiSPBC1198.02.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: PomBase
    • cytosol Source: PomBase
    • nucleus Source: PomBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi150 – 1501R → H in dea2-1: Abolishes adenine deaminase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Adenine deaminasePRO_0000256237Add
    BLAST

    Proteomic databases

    MaxQBiQ9P6I7.

    Interactioni

    Protein-protein interaction databases

    BioGridi276604. 1 interaction.
    MINTiMINT-4704041.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P6I7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000218813.
    KOiK01488.
    OMAiSPDFCGG.
    OrthoDBiEOG092C2UZH.
    PhylomeDBiQ9P6I7.

    Family and domain databases

    HAMAPiMF_01962. Adenine_deaminase. 1 hit.
    InterProiIPR001365. A/AMP_deaminase_dom.
    IPR028892. ADE.
    IPR006330. Ado/ade_deaminase.
    IPR032466. Metal_Hydrolase.
    [Graphical view]
    PfamiPF00962. A_deaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51556. SSF51556. 1 hit.
    TIGRFAMsiTIGR01430. aden_deam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9P6I7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSNLPIYNFI RKLPKCEHHV HLEGCLSPDL VFRLAKKNGI TLPSDDAAYT
    60 70 80 90 100
    TPSTLLASYE HFGCLDDFLR YYYIAVSVLI EASDFEALAY EYFSIAHSQG
    110 120 130 140 150
    VHHAEVFFDP QTHTSRGISY DVVVSGFSAA CERANRDFGM STNLIMCFLR
    160 170 180 190 200
    HLPSEAAHET FAEALKRNDF ENGIVAGVGL DSSEVDFPPE LFQEVYKLAA
    210 220 230 240 250
    EKGIRRTGHA GEEGDPSYIR SGLDNLSLQR IDHGIRLVED KELMKRVAEE
    260 270 280 290 300
    NIMLTMCPLS NLKLRCVNSI AELPVREFLE AGVPFSINCD DPAYFGGYTL
    310 320 330 340 350
    ENYFAIQKHF NLTVKEWVFI ANAAINGSWI SGKRKEELLS SVQKCVKEYT
    360
    AEIQQPKTLE TAVEVQA
    Length:367
    Mass (Da):41,199
    Last modified:October 1, 2000 - v1
    Checksum:i4470A2EF23405B85
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329671 Genomic DNA. Translation: CAB91177.1.
    RefSeqiNP_595071.1. NM_001020977.2.

    Genome annotation databases

    EnsemblFungiiSPBC1198.02.1; SPBC1198.02.1:pep; SPBC1198.02.
    GeneIDi2540066.
    KEGGispo:SPBC1198.02.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CU329671 Genomic DNA. Translation: CAB91177.1.
    RefSeqiNP_595071.1. NM_001020977.2.

    3D structure databases

    ProteinModelPortaliQ9P6I7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi276604. 1 interaction.
    MINTiMINT-4704041.

    Proteomic databases

    MaxQBiQ9P6I7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiSPBC1198.02.1; SPBC1198.02.1:pep; SPBC1198.02.
    GeneIDi2540066.
    KEGGispo:SPBC1198.02.

    Organism-specific databases

    EuPathDBiFungiDB:SPBC1198.02.
    PomBaseiSPBC1198.02.

    Phylogenomic databases

    HOGENOMiHOG000218813.
    KOiK01488.
    OMAiSPDFCGG.
    OrthoDBiEOG092C2UZH.
    PhylomeDBiQ9P6I7.

    Enzyme and pathway databases

    BRENDAi3.5.4.2. 5613.
    3.5.4.4. 5613.
    ReactomeiR-SPO-2161541. Abacavir metabolism.
    R-SPO-74217. Purine salvage.

    Miscellaneous databases

    PROiQ9P6I7.

    Family and domain databases

    HAMAPiMF_01962. Adenine_deaminase. 1 hit.
    InterProiIPR001365. A/AMP_deaminase_dom.
    IPR028892. ADE.
    IPR006330. Ado/ade_deaminase.
    IPR032466. Metal_Hydrolase.
    [Graphical view]
    PfamiPF00962. A_deaminase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51556. SSF51556. 1 hit.
    TIGRFAMsiTIGR01430. aden_deam. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiADE_SCHPO
    AccessioniPrimary (citable) accession number: Q9P6I7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: October 1, 2000
    Last modified: September 7, 2016
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.