Reviewed, UniProtKB/Swiss-Prot Q9P6I0 (SYNM_SCHPO)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase, mitochondrial
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	slm5
ORF Names:	SPBC1198.10c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).
Subcellular location	Mitochondrion. Ref.2
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro aspartyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Ref.2 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Asparaginyl-tRNA synthetase, mitochondrial		PRO_0000315955

Sequences

	Sequence		Length	Mass (Da)
	Q9P6I0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9F4E037F8157C46C	FASTA	441	50,508
	10 20 30 40 50 60 MNKFQLPKTL KSLWHHPHNG ELISINGWVR SIRKLKNVCF AMVSDGTCQQ ALQVVTSPEQ 70 80 90 100 110 120 SKKLSYGASV NIEGQLAVSK NAKLGLQQYE LLAEKIKIYG QINDDNYPIQ KKHLTTEMLR 130 140 150 160 170 180 QIPHLRLRTA KQGEIFRLRS DSLKALRQFF SSKDFTETNP PIITSSDCEG AGEVFTLTPQ 190 200 210 220 230 240 ETHKNKSFER DDQKHFFDRP AFLTVSTQLH LEALALGLSR VYTISPAFRA EQSHTSRHLA 250 260 270 280 290 300 EFWMLEAEVA FMTSLSQLTS LMEDMIKYTL NSLMEQNYHR DHWDQLLKPW KCMTYSEAIE 310 320 330 340 350 360 ELSAVKKTWK YPPKWGNDLS SEHEKYLCEI LHKTPVFVTD YPQKIKPFYM KSSGPDTVAA 370 380 390 400 410 420 VDLLVPQVGE LAGGSLRKDH LDEYKTYPPE LQWYLDLMKY SNAPHGGFGL GIERLIAFLE 430 440 GENTNVKETI PFPRSVGSIF A

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

[2]

"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB91185.1.
RefSeq	NP_595079.1.
3D structure databases
HSSP	HSSP built from PDB template 1B8A based on UniProtKB Q52428.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9P6I0.
Genome annotation databases
GeneID	2539632.
GenomeReviews	Gene locus slm5 in contig CU329671_GR.
KEGG	spo:SPBC1198.10c.
NMPDR	fig|4896.1.peg.945.
Organism-specific databases
GeneDB_Spombe	SPBC1198.10c.
Phylogenomic databases
OMA	VGELCGG.
Enzyme and pathway databases
BRENDA	6.1.1.22. 653.
Gene expression databases
ArrayExpress	Q9P6I0.
Family and domain databases
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYNM_SCHPO

Accession

Primary (citable) accession number: Q9P6I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)