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Reviewed, UniProtKB/Swiss-Prot Q9P4V4 (MEL_ZYGMR)

Last modified January 19, 2010. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
    MELr
Gene names
Name: MEL
OrganismZygosaccharomyces mrakii
Taxonomic identifier42260 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Zygosaccharomyces

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Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

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Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 470452Alpha-galactosidase
PRO_0000001012

Sites

Active site1491Nucleophile By similarity
Active site2091Proton donor By similarity

Amino acid modifications

Glycosylation431N-linked (GlcNAc...) Potential
Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation2701N-linked (GlcNAc...) Potential
Glycosylation3881N-linked (GlcNAc...) Potential
Glycosylation4131N-linked (GlcNAc...) Potential
Glycosylation4221N-linked (GlcNAc...) Potential
Glycosylation4351N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 74 By similarity
Disulfide bond121 ↔ 151 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9P4V4-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 232ACAE756DF432E

FASTA47051,912
        10         20         30         40         50         60 
MFSLLLLTST ALVETALGVS PSYNGLGLTP QMGWNNWNTF ACNVTEQLLL GTADRISELG 

        70         80         90        100        110        120 
LKDVGYNYVI LDDCWSGGRS SNGSLVPDLN KFPHGMKYVA DHLHDQDLLF GMYSSAGEYT 

       130        140        150        160        170        180 
CAGYPGSLGH EEKDAQFFAR NEVDYLKYDN CYNKGQFGTP QASYERYKAM SDALNNTGRP 

       190        200        210        220        230        240 
IFYSLCNWGQ DLTFYWGSAI ANSWRMSGDI TADFDRPDSR CPCGDDEYDC KYAGYHCSIM 

       250        260        270        280        290        300 
NILNKAAPMG QNANPGGWND LDMLEVGVGN LTDDEEKAHF SMWAMVRSPL IIGADVNHLK 

       310        320        330        340        350        360 
PSSFSIYAQS PVIAINQDPR GVPATRVWRR QVSDTDAYGR GEVQFWSGPL ENGDQVIAFL 

       370        380        390        400        410        420 
NGGNRMRPMN AGLDDIFFDS HPGAPELNST WAVYDLWANR MEDSVASDIL NGNRSSNGLL 

       430        440        450        460        470 
YNSTQQSYSQ GLARNDSRLF GSQIGTIQAG GRLNVTVPAH GVGLYRLRLQ

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References

[1]"Nucleotide sequence of alpha-galactosidase MEL gene from Zygosaccharomyces mrakii."
Oda Y., Fujisawa T.
Curr. Microbiol. 41:220-222(2000) [PubMed: 10915212] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 36242 / CBS 4218 / IFO 1835 / JCM 1800 / NRRL Y-12654.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB030209 Genomic DNA. Translation: BAA99555.1.

3D structure databases

SMRQ9P4V4. Positions 24-468.
ModBaseSearch...

Protein family/group databases

CAZyGH27. Glycoside Hydrolase Family 27.

Enzyme and pathway databases

BRENDA3.2.1.22. 290543.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR017853. Glyco_hydro_catalytic_core.
IPR000111. Glyco_hydro_GHD.
IPR006215. Glyco_hydro_melibiase.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMEL_ZYGMR
AccessionPrimary (citable) accession number: Q9P4V4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2000
Last modified: January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents