Q9P4V4 (MEL_ZYGMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-galactosidase
EC=3.2.1.22

Alternative name(s):
Alpha-D-galactoside galactohydrolase
MELr
Melibiase

Gene names

Name:

MEL

Organism

Zygosaccharomyces mrakii (Zygotorulaspora mrakii)

Taxonomic identifier

42260 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Zygosaccharomyces

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 470	452	Alpha-galactosidase		PRO_0000001012
Sites
	Active site	149	1	Nucleophile By similarity
	Active site	209	1	Proton donor By similarity
	Binding site	72	1	Substrate By similarity
	Binding site	73	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential
	Glycosylation	413	1	N-linked (GlcNAc...) Potential
	Glycosylation	422	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 74		By similarity
	Disulfide bond	121 ↔ 151		By similarity
	Disulfide bond	221 ↔ 237		By similarity
	Disulfide bond	223 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P4V4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 232ACAE756DF432E	FASTA	470	51,912
	10 20 30 40 50 60 MFSLLLLTST ALVETALGVS PSYNGLGLTP QMGWNNWNTF ACNVTEQLLL GTADRISELG 70 80 90 100 110 120 LKDVGYNYVI LDDCWSGGRS SNGSLVPDLN KFPHGMKYVA DHLHDQDLLF GMYSSAGEYT 130 140 150 160 170 180 CAGYPGSLGH EEKDAQFFAR NEVDYLKYDN CYNKGQFGTP QASYERYKAM SDALNNTGRP 190 200 210 220 230 240 IFYSLCNWGQ DLTFYWGSAI ANSWRMSGDI TADFDRPDSR CPCGDDEYDC KYAGYHCSIM 250 260 270 280 290 300 NILNKAAPMG QNANPGGWND LDMLEVGVGN LTDDEEKAHF SMWAMVRSPL IIGADVNHLK 310 320 330 340 350 360 PSSFSIYAQS PVIAINQDPR GVPATRVWRR QVSDTDAYGR GEVQFWSGPL ENGDQVIAFL 370 380 390 400 410 420 NGGNRMRPMN AGLDDIFFDS HPGAPELNST WAVYDLWANR MEDSVASDIL NGNRSSNGLL 430 440 450 460 470 YNSTQQSYSQ GLARNDSRLF GSQIGTIQAG GRLNVTVPAH GVGLYRLRLQ

References

[1]	"Nucleotide sequence of alpha-galactosidase MEL gene from Zygosaccharomyces mrakii." Oda Y., Fujisawa T. Curr. Microbiol. 41:220-222(2000) [PubMed: 10915212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 36242 / CBS 4218 / JCM 1800 / NBRC 1835 / NRRL Y-12654 / VTT C-94202.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB030209 Genomic DNA. Translation: BAA99555.1.
3D structure databases
ProteinModelPortal	Q9P4V4.
ModBase	Search...
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR006215. Glyco_hydro_melibiase. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27. PR00748. MELIBIASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MEL_ZYGMR

Accession

Primary (citable) accession number: Q9P4V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	October 1, 2000
Last modified:	September 21, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program