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Protein

Alpha-galactosidase

Gene

MEL

Organism
Zygotorulaspora mrakii (Zygosaccharomyces mrakii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Binding sitei73SubstrateBy similarity1
Binding sitei147SubstrateBy similarity1
Active sitei149NucleophileBy similarity1
Binding sitei205SubstrateBy similarity1
Active sitei209Proton donorBy similarity1
Binding sitei251SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
MELr
Melibiase
Gene namesi
Name:MEL
OrganismiZygotorulaspora mrakii (Zygosaccharomyces mrakii)
Taxonomic identifieri42260 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeZygotorulaspora

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000000101219 – 470Alpha-galactosidaseAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 74By similarity
Glycosylationi43N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi121 ↔ 151By similarity
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi221 ↔ 237By similarity
Disulfide bondi223 ↔ 230By similarity
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi388N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi413N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi435N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi454N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9P4V4.
SMRiQ9P4V4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR006215. Glyco_hydro_melibiase.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF16499. Melibiase_2. 1 hit.
PRINTSiPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00512. ALPHA_GALACTOSIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P4V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSLLLLTST ALVETALGVS PSYNGLGLTP QMGWNNWNTF ACNVTEQLLL
60 70 80 90 100
GTADRISELG LKDVGYNYVI LDDCWSGGRS SNGSLVPDLN KFPHGMKYVA
110 120 130 140 150
DHLHDQDLLF GMYSSAGEYT CAGYPGSLGH EEKDAQFFAR NEVDYLKYDN
160 170 180 190 200
CYNKGQFGTP QASYERYKAM SDALNNTGRP IFYSLCNWGQ DLTFYWGSAI
210 220 230 240 250
ANSWRMSGDI TADFDRPDSR CPCGDDEYDC KYAGYHCSIM NILNKAAPMG
260 270 280 290 300
QNANPGGWND LDMLEVGVGN LTDDEEKAHF SMWAMVRSPL IIGADVNHLK
310 320 330 340 350
PSSFSIYAQS PVIAINQDPR GVPATRVWRR QVSDTDAYGR GEVQFWSGPL
360 370 380 390 400
ENGDQVIAFL NGGNRMRPMN AGLDDIFFDS HPGAPELNST WAVYDLWANR
410 420 430 440 450
MEDSVASDIL NGNRSSNGLL YNSTQQSYSQ GLARNDSRLF GSQIGTIQAG
460 470
GRLNVTVPAH GVGLYRLRLQ
Length:470
Mass (Da):51,912
Last modified:October 1, 2000 - v1
Checksum:i232ACAE756DF432E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030209 Genomic DNA. Translation: BAA99555.1.

Similar proteinsi

Entry informationi

Entry nameiMEL_ZYGMR
AccessioniPrimary (citable) accession number: Q9P4V4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: October 1, 2000
Last modified: February 28, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program