Q9P419 (HOG1_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase hog1
Short name=MAP kinase hog1
EC=2.7.11.24

Gene names

Name:	hog1
Synonyms:	hogA, sakA
ORF Names:	AN1017

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Ref.2 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-171 and Tyr-173, which activates the enzyme By similarity. Phosphorylated by pbsB in response to osmotic and oxidative stresses. Ref.2 Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Mitogen-activated protein kinase hog1		PRO_0000289687
Regions
	Domain	20 – 313	294	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Motif	171 – 173	3	TXY
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Amino acid modifications
	Modified residue	171	1	Phosphothreonine By similarity
	Modified residue	173	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	132	1	V → C in AAF81523. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9P419 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 93DB6A450ECAB8E5	FASTA	379	43,183
	10 20 30 40 50 60 MAEFVRAQIF GTTFEITSRY TDLQPVGMGA FGLVCSARDQ LTAQPVAVKK IMKPFSTPVL 70 80 90 100 110 120 SKRTYRELKL LKHLRHENII SLSDIFISPL EDIYFVTELL GTDLHRLISS RPLEKQFIQY 130 140 150 160 170 180 FLYQIMRGLK YVHSAGVVHR DLKPSNILIN ENCDLKICDF GLARIQDPQM TGYVSTRYYR 190 200 210 220 230 240 APEIMLTWQK YDAKVDVWSA ACIFAEMLLG APLFPGKDHV NQFSIITELL GTPPDDVIQT 250 260 270 280 290 300 ICSENTLRFV KSLPKREPQD LAKLPKFLAL VHPDKKPEED EDYKNTINLL KAMLVYNPKD 310 320 330 340 350 360 RISAEAALAA PYLAPYHDET DEPVAEEKFD WSFNDADLPV DTWKIMMYSE ILDFHNIDQG 370 GDINPALVEG AGLNQQGFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Aspergillus nidulans hogA MAP kinase gene, homologous to the yeast HOG1 gene, is regulated by salt stress." Han K., Prade R.A. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"SakA MAP kinase is involved in stress signal transduction, sexual development and spore viability in Aspergillus nidulans." Kawasaki L., Sanchez O., Shiozaki K., Aguirre J. Mol. Microbiol. 45:1153-1163(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION.
[3]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[5]	"Aspergillus nidulans HOG pathway is activated only by two-component signalling pathway in response to osmotic stress." Furukawa K., Hoshi Y., Maeda T., Nakajima T., Abe K. Mol. Microbiol. 56:1246-1261(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF270498 Genomic DNA. Translation: AAF81523.1. AF282891 Genomic DNA. Translation: AAF97243.1. AACD01000015 Genomic DNA. Translation: EAA65585.1. BN001308 Genomic DNA. Translation: CBF88327.1.
RefSeq	XP_658621.1. XM_653529.1.
3D structure databases
HSSP	HSSP built from PDB template 2BAJ based on UniProtKB Q16539.
ProteinModelPortal	Q9P419.
ModBase	Search...
Proteomic databases
PRIDE	Q9P419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00001630; CADANIAP00001630; CADANIAG00001630.
GeneID	2876793.
KEGG	ani:AN1017.2.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
KO	K04441.
OMA	XVDLLEK.
OrthoDB	EOG496319.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_EMENI

Accession

Primary (citable) accession number: Q9P419
Secondary accession number(s): C8VU47, Q5BEL3, Q9P454

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program