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Protein

Transcription initiation factor IIE subunit alpha

Gene

tfa1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri124 – 15128C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • transcription initiation from RNA polymerase II promoter Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIE subunit alpha
Short name:
TFIIE-alpha
Gene namesi
Name:tfa1
ORF Names:SPAC458.07, SPAPYUG7.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC458.07.
PomBaseiSPAC458.07. tfa1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
  • transcription factor TFIIE complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Transcription initiation factor IIE subunit alphaPRO_0000211224Add
BLAST

Proteomic databases

MaxQBiQ9P3W1.

Interactioni

Subunit structurei

TFIIE is a tetramer of two alpha (tfa1) and two beta (tfa2) subunits.1 Publication

Protein-protein interaction databases

BioGridi280041. 2 interactions.
IntActiQ9P3W1. 1 interaction.
MINTiMINT-4703830.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 9992HTH TFE/IIEalpha-typePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi337 – 34610Asp/Glu-rich (acidic)
Compositional biasi420 – 43415Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the TFIIE alpha subunit family.Curated
Contains 1 HTH TFE/IIEalpha-type domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri124 – 15128C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiQ9P3W1.
KOiK03136.
OMAiDIVLPYD.
OrthoDBiEOG7R2BV4.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02002. TFIIE_alpha. 1 hit.
[Graphical view]
SMARTiSM00531. TFIIE. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P3W1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNAPEIVQR LIKMIMRAFY ETRHIIFMDA ILRHSALTDE QTALLMGIPI
60 70 80 90 100
KECRFIAGKL REDRLLAIQS RTEMKEGQQR QYHTTYFYID FCSTIDSIKW
110 120 130 140 150
RMHQLVKTVE DRMRNDFDSK GYVCPFCNKK FSSLDVLSLV TNEGTFACNV
160 170 180 190 200
CGTELKDDEE SAEMMSSQKR LGKLMGQVNG IIDALKRVDE IVVPQNNFQS
210 220 230 240 250
ALEHAVPVSL DTQNLSQQNL SKSNSDVRLS TSSPSITVDF SADKETDEKR
260 270 280 290 300
ERNCDKQVKA AQNILPEWHA TSTISGSITR AGAKDAALHS FRTETVNEVQ
310 320 330 340 350
DTKTDITSEK SALDAYYATL RAKQKEESEF MDSENVDDEE DDDFLDVTTA
360 370 380 390 400
TSLQNKSTDY GSVKRKTENL NSDSDIQNKR TKSIEENNSL PPIVSTNGIT
410 420 430
DGDTEMQESK KNVIINGFNE DDEDDEDEAD FEDV
Length:434
Mass (Da):49,117
Last modified:June 13, 2012 - v3
Checksum:iF8D4108A7254F249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237419 Genomic DNA. Translation: AAL55662.1.
AJ310560 mRNA. Translation: CAC32853.1.
AB176672 mRNA. Translation: BAD74158.1.
CU329670 Genomic DNA. Translation: CAB93849.3.
PIRiT50301.
RefSeqiNP_594701.3. NM_001020129.2.

Genome annotation databases

EnsemblFungiiSPAC458.07.1; SPAC458.07.1:pep; SPAC458.07.
GeneIDi2543627.
KEGGispo:SPAC458.07.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237419 Genomic DNA. Translation: AAL55662.1.
AJ310560 mRNA. Translation: CAC32853.1.
AB176672 mRNA. Translation: BAD74158.1.
CU329670 Genomic DNA. Translation: CAB93849.3.
PIRiT50301.
RefSeqiNP_594701.3. NM_001020129.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280041. 2 interactions.
IntActiQ9P3W1. 1 interaction.
MINTiMINT-4703830.

Proteomic databases

MaxQBiQ9P3W1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC458.07.1; SPAC458.07.1:pep; SPAC458.07.
GeneIDi2543627.
KEGGispo:SPAC458.07.

Organism-specific databases

EuPathDBiFungiDB:SPAC458.07.
PomBaseiSPAC458.07. tfa1.

Phylogenomic databases

InParanoidiQ9P3W1.
KOiK03136.
OMAiDIVLPYD.
OrthoDBiEOG7R2BV4.

Enzyme and pathway databases

ReactomeiR-SPO-674695. RNA Polymerase II Pre-transcription Events.
R-SPO-73776. RNA Polymerase II Promoter Escape.
R-SPO-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SPO-75953. RNA Polymerase II Transcription Initiation.
R-SPO-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.

Miscellaneous databases

PROiQ9P3W1.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR017919. TFIIE/TFIIEa_HTH.
IPR002853. TFIIE_asu.
IPR024550. TFIIEa/SarR/Rpc3_HTH_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02002. TFIIE_alpha. 1 hit.
[Graphical view]
SMARTiSM00531. TFIIE. 1 hit.
[Graphical view]
PROSITEiPS51344. HTH_TFE_IIE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Chromosomal localization of the rpb9+ and tfa1+ genes encoding components of the mRNA synthesis machinery of Schizosaccharomyces pombe."
    Shpakovski G.V., Baranova G.M.
    Bioorg. Khim. 26:623-630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBUNIT.
    Strain: 972 / ATCC 24843.
  2. "Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation."
    Hayashi K., Watanabe T., Tanaka A., Furumoto T., Sato-Tsuchiya C., Kimura M., Yokoi M., Ishihama A., Hanaoka F., Ohkuma Y.
    Genes Cells 10:207-224(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 972 / ATCC 24843.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Comparative functional genomics of the fission yeasts."
    Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N., Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y., Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K.
    , Bayne E.H., Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G., French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A., Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P., Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R., Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J., Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W., Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.
    Science 332:930-936(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiT2EA_SCHPO
AccessioniPrimary (citable) accession number: Q9P3W1
Secondary accession number(s): Q5R229, Q9C1W1, Q9P7Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: June 13, 2012
Last modified: July 6, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.