Reviewed, UniProtKB/Swiss-Prot Q9P3S1 (PPN1_NEUCR)

Names and origin

Protein names

Recommended name:
    Endopolyphosphatase
    EC=3.6.1.10

Gene names

Name:	ppn-1
ORF Names:	B24P7.140, NCU02996

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic polyphosphate (poly P) chains of many hundreds of phosphate residues into shorter lengths By similarity.
Catalytic activity	Polyphosphate + n H2O = (n+1) oligophosphate.
Subcellular location	Vacuole membrane; Single-pass type II membrane protein.
Post-translational modification	Processing by proteases in the vacuole may be required for activation By similarity.
Sequence caution	The sequence EAA36146.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane Vacuole
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	endopolyphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Endopolyphosphatase		PRO_0000058548
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 25	21	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 734	709	Vacuolar Potential
	Compositional bias	400 – 405	6	Poly-Lys
	Compositional bias	557 – 560	4	Poly-Glu
	Compositional bias	641 – 652	12	Poly-Glu
	Compositional bias	663 – 668	6	Poly-Glu
	Compositional bias	697 – 707	11	Poly-Lys
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	436	1	N-linked (GlcNAc...) Potential
	Glycosylation	537	1	N-linked (GlcNAc...) Potential
	Glycosylation	543	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9P3S1-1 [UniParc]. Last modified December 1, 2001. Version 2. Checksum: CA355CC65C9C1297	FASTA	734	83,974
	10 20 30 40 50 60 MSLSRCILGL ACLWHGVIAS PLGAVPSNIP IATDLQTAAE TVAQPIANSA RKLHGKFLHI 70 80 90 100 110 120 TGGQSRDDRK FYKPHSSTDE ADACHRGKGP AGVYGAEVSD CDSPFALINA TFDWIAANVK 130 140 150 160 170 180 DDIDFVIWTG DTARHDSDEG VPRNADQVLG TNRWIADKMA ELFSDSTGRH LEIPIVPTLG 190 200 210 220 230 240 NNDILPHNIL LPGPNSWLQH YTHIWRRFVP EAQRHSFQFG GWFYVEVIPN RLAIFSLNTL 250 260 270 280 290 300 YFFDRNAGTD GCASPSEPGY KQMEWLRIQL HIMRERGMKA ILMGHVPPAR TDSKKLWDEN 310 320 330 340 350 360 CWQKYSLWLR QYRDVVVSGV FGHMNIDHFF IHDERDINVG QLAGLADNSI DIREAMDDEL 370 380 390 400 410 420 SVTGAADYLR ELRQNWAKLQ PPPTDSKNSG QLKKGKKGRK GKKKKPDVWG ERYSLSLVSP 430 440 450 460 470 480 SIVPNYYPAL RIVEYNISGL EDTPVWRDAA KDAMSIELEQ NDRQKHLDLK RQHPSHMEDD 490 500 510 520 530 540 DEIDAQKKKG KKHKGGDSKP KKPDFLIPHP PAKSSPPGPA YSPQPLTLTG YTQYFANLTH 550 560 570 580 590 600 INNITTEASS ALLDHDEEEE TWVDWLLRWR KGRHGNRKPI HPKPDPREFQ FEVEYSTFND 610 620 630 640 650 660 KLYKLRDLTV KNYVELAYRI SKQPKKGKAK SIDVSYESAA EEEEEEEEEE EEDLFEEVEE 670 680 690 700 710 720 TDEEEEQEDD DLSDGEEVDD DSDEDELETE TFKKHDKKKH KKKKGKKRQN KVWMHFLTHA 730 FVSTVEKEDL KKFT

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL389890 Genomic DNA. Translation: CAB97279.2. AABX02000007 Genomic DNA. Translation: EAA36146.2. Sequence problems.
PIR	T50959.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.10. 266.
Family and domain databases
InterPro	IPR012358. EndopolyPtase_N1. [Graphical view]
PANTHER	PTHR10340:SF4. EndopolyPtase_N1. 1 hit.
PIRSF	PIRSF027093. EndopolyPtase_N1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPN1_NEUCR

Accession

Primary (citable) accession number: Q9P3S1
Secondary accession number(s): Q7RUX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	December 1, 2001
Last modified:	June 16, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)