Reviewed, UniProtKB/Swiss-Prot Q9P3R1 (ERG6_NEUCR)

Names and origin

Protein names

Recommended name:
    Sterol 24-C-methyltransferase
    EC=2.1.1.41
Alternative name(s):
    Delta(24)-sterol C-methyltransferase

Gene names

ORF Names:

B24P7.240, NCU03006

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 1/5.
Sequence similarities	Belongs to the methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sterol 24-C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Sterol 24-C-methyltransferase		PRO_0000124795

Sequences

	Sequence		Length	Mass (Da)
	Q9P3R1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: AE572C3EE6AE27E3	FASTA	379	42,457
	10 20 30 40 50 60 MPSTIALEKE DHQRDAEFKK VMHGDSAKAA GGVAALLKKN TEAQQIAVDE YFKHFDNKTA 70 80 90 100 110 120 EAETQADREA RTKEYATLTR HYYNLATDIY EYGWGQCFHF CRYSPGESFY QAIARHEHYL 130 140 150 160 170 180 AAQIGIKKDM KVLDVGCGVG GPAREIAKFT DAHITGLNNN DYQIDRATHY AVRDGLSGQL 190 200 210 220 230 240 KFVKGDFMQM SFPDNSFDAV YAIEATVHAP KLEGVYGEIY RVLKPGGTFG VYEWLMTDNY 250 260 270 280 290 300 DNDNVEHRDI RLAIEVGNGI SNMVTISEGL AAMKNVGFEL VHHEDLADRN DPMPWYWPIA 310 320 330 340 350 360 GELRYMQSYL DFFTVVRMTH TARRILHGFA GILETVGLAP KGTKKTADAL ARGADGLVAG 370 AKKKLFTPMY LMVGKKPLN

References

[1]

"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

[2]

"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL389890 Genomic DNA. Translation: CAB97289.1. AABX02000007 Genomic DNA. Translation: EAA36156.1.
PIR	T50969.
RefSeq	XP_965392.1.
3D structure databases
SMR	Q9P3R1. Positions 79-289, 87-316.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9P3R1.
Genome annotation databases
GeneID	3881542.
KEGG	ncr:NCU03006.
NMPDR	fig|5141.1.peg.8126.
Phylogenomic databases
eggNOG	fuNOG04306.
OrthoDB	EOG9T1K41.
PhylomeDB	Q9P3R1.
Enzyme and pathway databases
BRENDA	2.1.1.41. 266.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG6_NEUCR

Accession

Primary (citable) accession number: Q9P3R1
Secondary accession number(s): Q1K936

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)