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Protein

Endopolygalacturonase II

Gene

pgaII

Organism
Aspergillus awamori (Black koji mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

pH dependencei

Optimum pH is 5.0. Stable between pH 4.0 and 6.0.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei201 – 2011Proton donorPROSITE-ProRule annotation
Active sitei223 – 2231PROSITE-ProRule annotation

GO - Molecular functioni

  • polygalacturonase activity Source: UniProtKB

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28B_ASPAW.

Names & Taxonomyi

Protein namesi
Recommended name:
Endopolygalacturonase II (EC:3.2.1.15)
Short name:
EPG-II
Alternative name(s):
Pectinase 2
Polygalacturonase II
Short name:
PG-II
Polygalacturonase X2
Gene namesi
Name:pgaII
Synonyms:pg2, pgx2
OrganismiAspergillus awamori (Black koji mold)
Taxonomic identifieri105351 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 277Sequence analysisPRO_0000393628
Chaini28 – 362335Endopolygalacturonase IIPRO_0000393629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 45By similarity
Disulfide bondi203 ↔ 219By similarity
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Disulfide bondi329 ↔ 334By similarity
Disulfide bondi353 ↔ 362By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Inductioni

Expression depends of ambient pH (higher at high pHs and lower at low pHs), probably under the regulation of the pacC transcription factor.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9P359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati156 – 18631PbH1 1Add
BLAST
Repeati209 – 22921PbH1 2Add
BLAST
Repeati238 – 25922PbH1 3Add
BLAST
Repeati267 – 28923PbH1 4Add
BLAST
Repeati301 – 32222PbH1 5Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSFASLLRY GLAAGATLAS ASPIEARDSC TFTTAAAAKA GKAKCSTITL
60 70 80 90 100
DSIKVPAGTT LDLTGLTSGT KVIFEGTTTF DYEDWAGPLI SMSGKDITVT
110 120 130 140 150
GASGHLINCD GSRWWDGKGT SGKKKPKFFY AHGLDSSSIT GLNIKNTPLM
160 170 180 190 200
AFSVESDDIT LTDITINNAD GDSLGGHNTD AFDVGNSVGV NIIKPWVHNQ
210 220 230 240 250
DDCLAINSGE NIWFTGGTCI GGHGLSIGSV GDRSNNVVKN VTIEHSTVSN
260 270 280 290 300
SENAVRIKTI SGATGSVSEI TYSNIVMSGI SDYGVVIQQD YEDGKPTGKP
310 320 330 340 350
TNGVTITDVK LESVTGTVDS KATDIYLLCG SGSCSDWTWD DVKVTGGKKS
360
SACKNLPSVA SC
Length:362
Mass (Da):37,625
Last modified:October 1, 2000 - v1
Checksum:iE37F4447899C3AAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035081 Genomic DNA. Translation: BAA95407.1.
PIRiJC7374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035081 Genomic DNA. Translation: BAA95407.1.
PIRiJC7374.

3D structure databases

ProteinModelPortaliQ9P359.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28B_ASPAW.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR2_ASPAW
AccessioniPrimary (citable) accession number: Q9P359
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.