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Protein

Protein IMPACT

Gene

IMPACT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Stress response, Translation regulation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IMPACT
Alternative name(s):
Imprinted and ancient gene protein homolog
Gene namesi
Name:IMPACT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:20387. IMPACT.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485502.

Polymorphism and mutation databases

BioMutaiIMPACT.
DMDMi296434540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Protein IMPACTPRO_0000330850Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei297 – 2971PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P2X3.
MaxQBiQ9P2X3.
PaxDbiQ9P2X3.
PeptideAtlasiQ9P2X3.
PRIDEiQ9P2X3.

PTM databases

iPTMnetiQ9P2X3.

Expressioni

Tissue specificityi

Widely expressed. Expressed at high level in brain.2 Publications

Gene expression databases

BgeeiQ9P2X3.
CleanExiHS_IMPACT.
ExpressionAtlasiQ9P2X3. baseline and differential.
GenevisibleiQ9P2X3. HS.

Organism-specific databases

HPAiHPA041045.
HPA041968.

Interactioni

Subunit structurei

Interacts with GCN1; prevents the interaction of GCN1 with EIF2AK4/GCN2 and inhibits EIF2AK4/GCN2 kinase activity. Interaction with RPL39; this interaction occurs in a GCN1-independent manner. Associates with ribosomes; this interaction occurs in a GCN1-independent manner. Associates with actin; this interaction occurs in a GCN1-independent manner.By similarity

Protein-protein interaction databases

BioGridi120642. 32 interactions.
IntActiQ9P2X3. 29 interactions.
STRINGi9606.ENSP00000284202.

Structurei

3D structure databases

ProteinModelPortaliQ9P2X3.
SMRiQ9P2X3. Positions 182-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 116103RWDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the IMPACT family.Curated
Contains 1 RWD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3299. Eukaryota.
COG1739. LUCA.
GeneTreeiENSGT00390000017571.
HOGENOMiHOG000160430.
HOVERGENiHBG108005.
InParanoidiQ9P2X3.
OMAiIFCIKIS.
OrthoDBiEOG7V766R.
PhylomeDBiQ9P2X3.
TreeFamiTF314823.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.230.30. 1 hit.
InterProiIPR023582. Impact.
IPR001498. Impact_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR020569. UPF0029_Impact_CS.
[Graphical view]
PANTHERiPTHR16301. PTHR16301. 1 hit.
PfamiPF05773. RWD. 1 hit.
PF01205. UPF0029. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
PS00910. UPF0029. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2X3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW
60 70 80 90 100
TLCLQVMLPN EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES
110 120 130 140 150
ILYLWVEKIR DVLIQKSQMT EPGPDVKKKT EEEDVECEDD LILACQPESS
160 170 180 190 200
LKALDFDISE TRTEVEVEEL PPIDHGIPIT DRRSTFQAHL APVVCPKQVK
210 220 230 240 250
MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG ETAAGGRLLH
260 270 280 290 300
LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE
310 320
SSKALGKNKK VRKDKKRNEH
Length:320
Mass (Da):36,476
Last modified:May 18, 2010 - v2
Checksum:i75AAB91E00594212
GO
Isoform 2 (identifier: Q9P2X3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.

Note: No experimental confirmation available.
Show »
Length:202
Mass (Da):22,999
Checksum:i921D01A97FA48495
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti125 – 1251D → E.
Corresponds to variant rs582234 [ dbSNP | Ensembl ].
VAR_042723
Natural varianti151 – 1511L → V.4 Publications
Corresponds to variant rs677688 [ dbSNP | Ensembl ].
VAR_042724

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 118118Missing in isoform 2. 1 PublicationVSP_033136Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232229 Genomic DNA. Translation: AAG23917.1.
AB026264 mRNA. Translation: BAA95160.1.
AF208694 mRNA. Translation: AAG35736.1.
AK292533 mRNA. Translation: BAF85222.1.
AC007922 Genomic DNA. No translation available.
AC020937 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01186.1.
BC034016 mRNA. Translation: AAH34016.1.
BC036074 mRNA. Translation: AAH36074.1.
CCDSiCCDS11886.1. [Q9P2X3-1]
RefSeqiNP_060909.1. NM_018439.3.
UniGeneiHs.515317.

Genome annotation databases

EnsembliENST00000284202; ENSP00000284202; ENSG00000154059. [Q9P2X3-1]
GeneIDi55364.
KEGGihsa:55364.
UCSCiuc002kvh.5. human. [Q9P2X3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232229 Genomic DNA. Translation: AAG23917.1.
AB026264 mRNA. Translation: BAA95160.1.
AF208694 mRNA. Translation: AAG35736.1.
AK292533 mRNA. Translation: BAF85222.1.
AC007922 Genomic DNA. No translation available.
AC020937 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01186.1.
BC034016 mRNA. Translation: AAH34016.1.
BC036074 mRNA. Translation: AAH36074.1.
CCDSiCCDS11886.1. [Q9P2X3-1]
RefSeqiNP_060909.1. NM_018439.3.
UniGeneiHs.515317.

3D structure databases

ProteinModelPortaliQ9P2X3.
SMRiQ9P2X3. Positions 182-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120642. 32 interactions.
IntActiQ9P2X3. 29 interactions.
STRINGi9606.ENSP00000284202.

PTM databases

iPTMnetiQ9P2X3.

Polymorphism and mutation databases

BioMutaiIMPACT.
DMDMi296434540.

Proteomic databases

EPDiQ9P2X3.
MaxQBiQ9P2X3.
PaxDbiQ9P2X3.
PeptideAtlasiQ9P2X3.
PRIDEiQ9P2X3.

Protocols and materials databases

DNASUi55364.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000284202; ENSP00000284202; ENSG00000154059. [Q9P2X3-1]
GeneIDi55364.
KEGGihsa:55364.
UCSCiuc002kvh.5. human. [Q9P2X3-1]

Organism-specific databases

CTDi55364.
GeneCardsiIMPACT.
HGNCiHGNC:20387. IMPACT.
HPAiHPA041045.
HPA041968.
MIMi615319. gene.
neXtProtiNX_Q9P2X3.
PharmGKBiPA143485502.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3299. Eukaryota.
COG1739. LUCA.
GeneTreeiENSGT00390000017571.
HOGENOMiHOG000160430.
HOVERGENiHBG108005.
InParanoidiQ9P2X3.
OMAiIFCIKIS.
OrthoDBiEOG7V766R.
PhylomeDBiQ9P2X3.
TreeFamiTF314823.

Miscellaneous databases

ChiTaRSiIMPACT. human.
GenomeRNAii55364.
PROiQ9P2X3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9P2X3.
CleanExiHS_IMPACT.
ExpressionAtlasiQ9P2X3. baseline and differential.
GenevisibleiQ9P2X3. HS.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.30.230.30. 1 hit.
InterProiIPR023582. Impact.
IPR001498. Impact_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR006575. RWD-domain.
IPR016135. UBQ-conjugating_enzyme/RWD.
IPR020569. UPF0029_Impact_CS.
[Graphical view]
PANTHERiPTHR16301. PTHR16301. 1 hit.
PfamiPF05773. RWD. 1 hit.
PF01205. UPF0029. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF54495. SSF54495. 1 hit.
PROSITEiPS50908. RWD. 1 hit.
PS00910. UPF0029. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative genome analysis of the mouse imprinted gene impact and its nonimprinted human homolog IMPACT: toward the structural basis for species-specific imprinting."
    Okamura K., Hagiwara-Takeuchi Y., Li T., Vu T.H., Hirai M., Hattori M., Sakaki Y., Hoffman A.R., Ito T.
    Genome Res. 10:1878-1889(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), LACK OF IMPRINTING, TISSUE SPECIFICITY, VARIANT VAL-151.
  2. "Human homolog of the mouse imprinted gene Impact resides at the pericentric region of chromosome 18 within the critical region for bipolar affective disorder."
    Kosaki K., Suzuki T., Kosaki R., Yoshihashi H., Itoh M., Goto Y., Matsuo N.
    Mol. Psychiatry 6:87-91(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-151.
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-151.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT VAL-151.
    Tissue: Brain and Lung.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiIMPCT_HUMAN
AccessioniPrimary (citable) accession number: Q9P2X3
Secondary accession number(s): A8MXG0, Q49AM0, Q9H2X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 18, 2010
Last modified: July 6, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In contrast to the mouse or rabbit ortholog, the IMPACT locus is not imprinted in human.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.