Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

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Q9P2W7 (B3GA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (7) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
EC=2.4.1.135

Alternative name(s):
Beta-1,3-glucuronyltransferase 1
Glucuronosyltransferase P
Short name=GlcAT-P
UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Short name=GlcUAT-P

Gene names

Name:	B3GAT1
Synonyms:	GLCATP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group By similarity.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Mainly expressed in the brain.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Traceable author statement. Source: ProtInc
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement. Source: ProtInc
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 334

334

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

PRO_0000195167

Regions

Topological domain

1 – 6

Cytoplasmic Potential

Transmembrane

7 – 27

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

28 – 334

307

Lumenal Potential

Sites

Active site

284

Proton acceptor By similarity

Metal binding

197

Manganese By similarity

Amino acid modifications

Glycosylation

140

N-linked (GlcNAc...) Potential

Glycosylation

184

N-linked (GlcNAc...) Potential

Glycosylation

303

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

131

A → T.
Corresponds to variant rs35434644 [ dbSNP | Ensembl ].

VAR_044538

Experimental info

Sequence conflict

240

G → R in BAA96077. Ref.1

Secondary structure

334

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9P2W7 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: 0DF42399D19701B3
Show »

FASTA

334

38,256

        10         20         30         40         50         60 
MPKRRDILAI VLIVLPWTLL ITVWHQSTLA PLLAVHKDEG SDPRRETPPG ADPREYCTSD 

        70         80         90        100        110        120 
RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA ELTRMANTLL HVPNLHWLVV 

       130        140        150        160        170        180 
EDAPRRTPLT ARLLRDTGLN YTHLHVETPR NYKLRGDARD PRIPRGTMQR NLALRWLRET 

       190        200        210        220        230        240 
FPRNSSQPGV VYFADDDNTY SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG 

       250        260        270        280        290        300 
WKTVFDPHRP FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK 

       310        320        330 
AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of human glucuronyltransferase involved in biosynthesis of the HNK-1 carbohydrate epitope." Mitsumoto Y., Oka S., Sakuma H., Inazawa J., Kawasaki T. Genomics 65:166-173(2000) [PubMed: 10783264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1." Kakuda S., Shiba T., Ishiguro M., Tagawa H., Oka S., Kajihara Y., Kawasaki T., Wakatsuki S., Kato R. J. Biol. Chem. 279:22693-22703(2004) [PubMed: 14993226] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 83-334.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB029396 mRNA. Translation: BAA96077.1.
CR457098 mRNA. Translation: CAG33379.1.
BC010466 mRNA. Translation: AAH10466.1.

IPI

IPI01013111.

RefSeq

NP_061114.2. NM_018644.3.
NP_473366.1. NM_054025.2.

UniGene

Hs.381050.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V82	X-ray	1.85	A/B	83-334	[»]
1V83	X-ray	1.90	A/B	83-334	[»]
1V84	X-ray	1.82	A/B	83-334	[»]

ProteinModelPortal

Q9P2W7.

SMR

Q9P2W7. Positions 83-334.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9P2W7. 2 interactions.

STRING

Q9P2W7.

Protein family/group databases

CAZy

GT43. Glycosyltransferase Family 43.

PTM databases

PhosphoSite

Q9P2W7.

Polymorphism databases

DMDM

205830910.

Proteomic databases

PRIDE

Q9P2W7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000312527; ENSP00000307875; ENSG00000109956.
ENST00000392580; ENSP00000376359; ENSG00000109956.

GeneID

27087.

KEGG

hsa:27087.

Organism-specific databases

CTD

27087.

GeneCards

GC11M134281.

H-InvDB

HIX0010301.

HGNC

HGNC:921. B3GAT1.

HPA

CAB002500.
CAB010893.

MIM

151290. gene.

neXtProt

NX_Q9P2W7.

PharmGKB

PA25215.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00390000017640.

HOVERGEN

HBG050650.

InParanoid

Q9P2W7.

OMA

ADPREYC.

OrthoDB

EOG4XPQG4.

PhylomeDB

Q9P2W7.

Enzyme and pathway databases

BioCyc

MetaCyc:HS03272-MONOMER.

BRENDA

2.4.1.135. 2681.

Gene expression databases

ArrayExpress

Q9P2W7.

Bgee

Q9P2W7.

CleanEx

HS_B3GAT1.

Genevestigator

Q9P2W7.

GermOnline

ENSG00000109956. Homo sapiens.

Family and domain databases

InterPro

IPR005027. Glyco_trans_43.
[Graphical view]

K00735.

PANTHER

PTHR10896. Glyco_trans_43. 1 hit.

Pfam

PF03360. Glyco_transf_43. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

49699.

SOURCE

Search...

Entry information

Entry name

B3GA1_HUMAN

Accession

Primary (citable) accession number: Q9P2W7
Secondary accession number(s): Q96FS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents