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Q9P2U7 (VGLU1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular glutamate transporter 1

Short name=VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene names
Name:SLC17A7
Synonyms:BNPI, VGLUT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.1

Subunit structure

Interacts with SHANK3 By similarity.

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity. Membrane; Multi-pass membrane protein Potential. Cell junctionsynapsesynaptosome By similarity.

Tissue specificity

Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. Ref.1

Sequence similarities

Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
   Biological processIon transport
Neurotransmitter transport
Sodium transport
Symport
Transport
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-glutamate import

Inferred from electronic annotation. Source: Ensembl

glutamate secretion

Traceable author statement. Source: Reactome

ion transport

Traceable author statement. Source: Reactome

long-term memory

Inferred from electronic annotation. Source: Ensembl

neurotransmitter secretion

Traceable author statement. Source: Reactome

phosphate ion transmembrane transport

Inferred from direct assay Ref.1. Source: GOC

phosphate ion transport

Traceable author statement PubMed 8632143. Source: ProtInc

regulation of excitatory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

sequestering of neurotransmitter

Inferred from electronic annotation. Source: Ensembl

sodium ion transmembrane transport

Inferred from direct assay Ref.1. Source: GOC

synaptic transmission

Traceable author statement. Source: Reactome

synaptic transmission, glutamatergic

Inferred from electronic annotation. Source: Ensembl

transmembrane transport

Traceable author statement. Source: Reactome

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

clathrin-sculpted glutamate transport vesicle membrane

Traceable author statement. Source: Reactome

excitatory synapse

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from direct assay Ref.1. Source: MGI

neuron projection

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Traceable author statement. Source: Reactome

presynaptic active zone

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle membrane

Traceable author statement. Source: Reactome

   Molecular_functionL-glutamate transmembrane transporter activity

Traceable author statement. Source: Reactome

inorganic phosphate transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

sodium-dependent phosphate transmembrane transporter activity

Traceable author statement PubMed 8632143. Source: ProtInc

sodium:inorganic phosphate symporter activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Vesicular glutamate transporter 1
PRO_0000331611

Regions

Topological domain1 – 6363Cytoplasmic Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 11632Extracellular Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 1403Cytoplasmic Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 1698Extracellular Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 20818Cytoplasmic Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 2367Extracellular Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 30245Cytoplasmic Potential
Transmembrane303 – 32321Helical; Potential
Topological domain324 – 34118Extracellular Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 37816Cytoplasmic Potential
Transmembrane379 – 39921Helical; Potential
Topological domain400 – 4012Extracellular Potential
Transmembrane402 – 42221Helical; Potential
Topological domain423 – 43513Cytoplasmic Potential
Transmembrane436 – 45621Helical; Potential
Topological domain457 – 46913Extracellular Potential
Transmembrane470 – 49021Helical; Potential
Topological domain491 – 56070Cytoplasmic Potential
Compositional bias530 – 55627Pro-rich

Natural variations

Natural variant4311P → R. Ref.3
Corresponds to variant rs17855709 [ dbSNP | Ensembl ].
VAR_042904

Sequences

Sequence LengthMass (Da)Tools
Q9P2U7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C88DAFB34B6E45B6

FASTA56061,613
        10         20         30         40         50         60 
MEFRQEEFRK LAGRALGKLH RLLEKRQEGA ETLELSADGR PVTTQTRDPP VVDCTCFGLP 

        70         80         90        100        110        120 
RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFS WDPETVGLIH 

       130        140        150        160        170        180 
GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 

       190        200        210        220        230        240 
LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 

       250        260        270        280        290        300 
YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPLTK FSTPWRRFFT 

       310        320        330        340        350        360 
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 

       370        380        390        400        410        420 
IADFLRSRRI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 

       430        440        450        460        470        480 
GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 

       490        500        510        520        530        540 
GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD DSEMEDEAEP PGAPPAPPPS 

       550        560 
YGATHSTFQP PRPPPPVRDY 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter."
Aihara Y., Mashima H., Onda H., Hisano S., Kasuya H., Hori T., Yamada S., Tomura H., Yamada Y., Inoue I., Kojima I., Takeda J.
J. Neurochem. 74:2622-2625(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Brain.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-431.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB032436 mRNA. Translation: BAA92875.1.
CH471177 Genomic DNA. Translation: EAW52485.1.
BC059379 mRNA. Translation: AAH59379.1.
RefSeqNP_064705.1. NM_020309.3.
UniGeneHs.375616.

3D structure databases

ProteinModelPortalQ9P2U7.
SMRQ9P2U7. Positions 123-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000221485.

Protein family/group databases

TCDB2.A.1.14.30. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteQ9P2U7.

Polymorphism databases

DMDM74725387.

Proteomic databases

PaxDbQ9P2U7.
PRIDEQ9P2U7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000221485; ENSP00000221485; ENSG00000104888.
GeneID57030.
KEGGhsa:57030.
UCSCuc002pnp.3. human.

Organism-specific databases

CTD57030.
GeneCardsGC19M049933.
HGNCHGNC:16704. SLC17A7.
HPACAB037183.
HPA050458.
MIM605208. gene.
neXtProtNX_Q9P2U7.
PharmGKBPA423.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000230812.
HOVERGENHBG008834.
InParanoidQ9P2U7.
KOK12302.
OMAMHGHVVI.
OrthoDBEOG789C9Z.
PhylomeDBQ9P2U7.
TreeFamTF313535.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.

Gene expression databases

ArrayExpressQ9P2U7.
BgeeQ9P2U7.
CleanExHS_SLC17A7.
GenevestigatorQ9P2U7.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiVesicular_glutamate_transporter_1.
GenomeRNAi57030.
NextBio62781.
PROQ9P2U7.
SOURCESearch...

Entry information

Entry nameVGLU1_HUMAN
AccessionPrimary (citable) accession number: Q9P2U7
Secondary accession number(s): Q6PCD0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM