Q9P2U7 (VGLU1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicular glutamate transporter 1
Short name=VGluT1

Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7

Gene names

Name:	SLC17A7
Synonyms:	BNPI, VGLUT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.1
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane By similarity. Membrane; Multi-pass membrane protein Potential. Cell junction › synapse › synaptosome By similarity.
Tissue specificity	Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. Ref.1
Sequence similarities	Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
Biological process	Ion transport Neurotransmitter transport Sodium transport Symport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-glutamate import Inferred from electronic annotation. Source: Compara glutamate secretion Traceable author statement. Source: Reactome long-term memory Inferred from electronic annotation. Source: Compara neurotransmitter secretion Traceable author statement. Source: Reactome regulation of excitatory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara sequestering of neurotransmitter Inferred from electronic annotation. Source: Compara synaptic transmission, glutamatergic Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW clathrin-sculpted glutamate transport vesicle membrane Traceable author statement. Source: Reactome excitatory synapse Inferred from electronic annotation. Source: Compara integral to membrane Inferred from direct assay Ref.1. Source: MGI neuron projection Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Traceable author statement. Source: Reactome presynaptic active zone Inferred from electronic annotation. Source: Compara synaptic vesicle membrane Traceable author statement. Source: Reactome
Molecular_function	L-glutamate transmembrane transporter activity Traceable author statement. Source: Reactome inorganic phosphate transmembrane transporter activity Inferred from electronic annotation. Source: Compara sodium-dependent phosphate transmembrane transporter activity Traceable author statement PubMed 8632143. Source: ProtInc sodium:inorganic phosphate symporter activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Vesicular glutamate transporter 1		PRO_0000331611
Regions
	Topological domain	1 – 63	63	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 116	32	Extracellular Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Topological domain	138 – 140	3	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Topological domain	162 – 169	8	Extracellular Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Topological domain	191 – 208	18	Cytoplasmic Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Topological domain	230 – 236	7	Extracellular Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Topological domain	258 – 302	45	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Topological domain	324 – 341	18	Extracellular Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Topological domain	363 – 378	16	Cytoplasmic Potential
	Transmembrane	379 – 399	21	Helical; Potential
	Topological domain	400 – 401	2	Extracellular Potential
	Transmembrane	402 – 422	21	Helical; Potential
	Topological domain	423 – 435	13	Cytoplasmic Potential
	Transmembrane	436 – 456	21	Helical; Potential
	Topological domain	457 – 469	13	Extracellular Potential
	Transmembrane	470 – 490	21	Helical; Potential
	Topological domain	491 – 560	70	Cytoplasmic Potential
	Compositional bias	530 – 556	27	Pro-rich
Natural variations
	Natural variant	431	1	P → R. Ref.3 Corresponds to variant rs17855709 [ dbSNP | Ensembl ].		VAR_042904

Sequences

	Sequence		Length	Mass (Da)
	Q9P2U7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C88DAFB34B6E45B6	FASTA	560	61,613
	10 20 30 40 50 60 MEFRQEEFRK LAGRALGKLH RLLEKRQEGA ETLELSADGR PVTTQTRDPP VVDCTCFGLP 70 80 90 100 110 120 RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFS WDPETVGLIH 130 140 150 160 170 180 GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 190 200 210 220 230 240 LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 250 260 270 280 290 300 YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPLTK FSTPWRRFFT 310 320 330 340 350 360 SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 370 380 390 400 410 420 IADFLRSRRI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 430 440 450 460 470 480 GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 490 500 510 520 530 540 GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD DSEMEDEAEP PGAPPAPPPS 550 560 YGATHSTFQP PRPPPPVRDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter." Aihara Y., Mashima H., Onda H., Hisano S., Kasuya H., Hori T., Yamada S., Tomura H., Yamada Y., Inoue I., Kojima I., Takeda J. J. Neurochem. 74:2622-2625(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-431. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB032436 mRNA. Translation: BAA92875.1. CH471177 Genomic DNA. Translation: EAW52485.1. BC059379 mRNA. Translation: AAH59379.1.
IPI	IPI00025331.
RefSeq	NP_064705.1. NM_020309.3.
UniGene	Hs.375616.
3D structure databases
ProteinModelPortal	Q9P2U7.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000221485.
PTM databases
PhosphoSite	Q9P2U7.
Polymorphism databases
DMDM	74725387.
Proteomic databases
PaxDb	Q9P2U7.
PRIDE	Q9P2U7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000221485; ENSP00000221485; ENSG00000104888.
GeneID	57030.
KEGG	hsa:57030.
UCSC	uc002pnp.3. human.
Organism-specific databases
CTD	57030.
GeneCards	GC19M049933.
HGNC	HGNC:16704. SLC17A7.
HPA	CAB037183.
MIM	605208. gene.
neXtProt	NX_Q9P2U7.
PharmGKB	PA423.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000230812.
HOVERGEN	HBG008834.
InParanoid	Q9P2U7.
KO	K12302.
OMA	MHGHVVI.
OrthoDB	EOG4XWFXC.
PhylomeDB	Q9P2U7.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System. REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
ArrayExpress	Q9P2U7.
Bgee	Q9P2U7.
CleanEx	HS_SLC17A7.
Genevestigator	Q9P2U7.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57030.
NextBio	62781.
SOURCE	Search...

Entry information

Entry name

VGLU1_HUMAN

Accession

Primary (citable) accession number: Q9P2U7
Secondary accession number(s): Q6PCD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.