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Protein

Vesicular glutamate transporter 1

Gene

SLC17A7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.1 Publication

GO - Molecular functioni

  1. inorganic phosphate transmembrane transporter activity Source: Ensembl
  2. L-glutamate transmembrane transporter activity Source: Reactome
  3. sodium:inorganic phosphate symporter activity Source: MGI
  4. sodium-dependent phosphate transmembrane transporter activity Source: ProtInc

GO - Biological processi

  1. glutamate secretion Source: Reactome
  2. ion transport Source: Reactome
  3. L-glutamate import Source: Ensembl
  4. L-glutamate transmembrane transport Source: GOC
  5. long-term memory Source: Ensembl
  6. neurotransmitter secretion Source: Reactome
  7. phosphate ion transmembrane transport Source: GOC
  8. phosphate ion transport Source: ProtInc
  9. regulation of excitatory postsynaptic membrane potential Source: Ensembl
  10. sequestering of neurotransmitter Source: Ensembl
  11. sodium ion transmembrane transport Source: GOC
  12. synaptic transmission Source: Reactome
  13. synaptic transmission, glutamatergic Source: Ensembl
  14. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19372. Organic anion transporters.

Protein family/group databases

TCDBi2.A.1.14.30. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 1
Short name:
VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene namesi
Name:SLC17A7
Synonyms:BNPI, VGLUT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:16704. SLC17A7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6363CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Topological domaini85 – 11632ExtracellularSequence AnalysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence AnalysisAdd
BLAST
Topological domaini138 – 1403CytoplasmicSequence Analysis
Transmembranei141 – 16121HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 1698ExtracellularSequence Analysis
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Topological domaini191 – 20818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence AnalysisAdd
BLAST
Topological domaini230 – 2367ExtracellularSequence Analysis
Transmembranei237 – 25721HelicalSequence AnalysisAdd
BLAST
Topological domaini258 – 30245CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Topological domaini363 – 37816CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei379 – 39921HelicalSequence AnalysisAdd
BLAST
Topological domaini400 – 4012ExtracellularSequence Analysis
Transmembranei402 – 42221HelicalSequence AnalysisAdd
BLAST
Topological domaini423 – 43513CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei436 – 45621HelicalSequence AnalysisAdd
BLAST
Topological domaini457 – 46913ExtracellularSequence AnalysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence AnalysisAdd
BLAST
Topological domaini491 – 56070CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cerebellar mossy fiber Source: Ensembl
  3. clathrin-sculpted glutamate transport vesicle membrane Source: Reactome
  4. excitatory synapse Source: Ensembl
  5. integral component of membrane Source: MGI
  6. plasma membrane Source: Reactome
  7. presynaptic active zone Source: Ensembl
  8. synaptic vesicle membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA423.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Vesicular glutamate transporter 1PRO_0000331611Add
BLAST

Proteomic databases

PaxDbiQ9P2U7.
PRIDEiQ9P2U7.

PTM databases

PhosphoSiteiQ9P2U7.

Expressioni

Tissue specificityi

Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.1 Publication

Gene expression databases

BgeeiQ9P2U7.
CleanExiHS_SLC17A7.
ExpressionAtlasiQ9P2U7. baseline and differential.
GenevestigatoriQ9P2U7.

Organism-specific databases

HPAiCAB037183.
HPA050458.
HPA063679.

Interactioni

Subunit structurei

Interacts with SHANK3.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000221485.

Structurei

3D structure databases

ProteinModelPortaliQ9P2U7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi530 – 55627Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ9P2U7.
KOiK12302.
OMAiPLTKFNT.
OrthoDBiEOG789C9Z.
PhylomeDBiQ9P2U7.
TreeFamiTF313535.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2U7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEFRQEEFRK LAGRALGKLH RLLEKRQEGA ETLELSADGR PVTTQTRDPP
60 70 80 90 100
VVDCTCFGLP RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG
110 120 130 140 150
HVVVQKAQFS WDPETVGLIH GSFFWGYIVT QIPGGFICQK FAANRVFGFA
160 170 180 190 200
IVATSTLNML IPSAARVHYG CVIFVRILQG LVEGVTYPAC HGIWSKWAPP
210 220 230 240 250
LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV YGSFGIFWYL
260 270 280 290 300
FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPLTK FSTPWRRFFT
310 320 330 340 350
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV
360 370 380 390 400
MTIIVPIGGQ IADFLRSRRI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS
410 420 430 440 450
KGVAISFLVL AVGFSGFAIS GFNVNHLDIA PRYASILMGI SNGVGTLSGM
460 470 480 490 500
VCPIIVGAMT KHKTREEWQY VFLIASLVHY GGVIFYGVFA SGEKQPWAEP
510 520 530 540 550
EEMSEEKCGF VGHDQLAGSD DSEMEDEAEP PGAPPAPPPS YGATHSTFQP
560
PRPPPPVRDY
Length:560
Mass (Da):61,613
Last modified:October 1, 2000 - v1
Checksum:iC88DAFB34B6E45B6
GO
Isoform 2 (identifier: Q9P2U7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Note: No experimental confirmation available.

Show »
Length:493
Mass (Da):53,946
Checksum:i6333DBF37546389F
GO
Isoform 3 (identifier: Q9P2U7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MEFRQEEFRKLAGRALGKLHR → MGSQLALAG

Note: No experimental confirmation available.

Show »
Length:548
Mass (Da):59,887
Checksum:i4FFF0B4E78E6BE69
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti431 – 4311P → R.1 Publication
Corresponds to variant rs17855709 [ dbSNP | Ensembl ].
VAR_042904

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6767Missing in isoform 2. 1 PublicationVSP_056110Add
BLAST
Alternative sequencei1 – 2121MEFRQ…GKLHR → MGSQLALAG in isoform 3. 1 PublicationVSP_056111Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032436 mRNA. Translation: BAA92875.1.
AK294226 mRNA. Translation: BAG57530.1.
AK294405 mRNA. Translation: BAG57657.1.
AC010619 Genomic DNA. No translation available.
CH471177 Genomic DNA. Translation: EAW52485.1.
BC059379 mRNA. Translation: AAH59379.1.
CCDSiCCDS12764.1. [Q9P2U7-1]
RefSeqiNP_064705.1. NM_020309.3. [Q9P2U7-1]
UniGeneiHs.375616.

Genome annotation databases

EnsembliENST00000221485; ENSP00000221485; ENSG00000104888. [Q9P2U7-1]
ENST00000600601; ENSP00000470338; ENSG00000104888. [Q9P2U7-2]
GeneIDi57030.
KEGGihsa:57030.
UCSCiuc002pnp.3. human. [Q9P2U7-1]

Polymorphism databases

DMDMi74725387.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032436 mRNA. Translation: BAA92875.1.
AK294226 mRNA. Translation: BAG57530.1.
AK294405 mRNA. Translation: BAG57657.1.
AC010619 Genomic DNA. No translation available.
CH471177 Genomic DNA. Translation: EAW52485.1.
BC059379 mRNA. Translation: AAH59379.1.
CCDSiCCDS12764.1. [Q9P2U7-1]
RefSeqiNP_064705.1. NM_020309.3. [Q9P2U7-1]
UniGeneiHs.375616.

3D structure databases

ProteinModelPortaliQ9P2U7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000221485.

Protein family/group databases

TCDBi2.A.1.14.30. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteiQ9P2U7.

Polymorphism databases

DMDMi74725387.

Proteomic databases

PaxDbiQ9P2U7.
PRIDEiQ9P2U7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221485; ENSP00000221485; ENSG00000104888. [Q9P2U7-1]
ENST00000600601; ENSP00000470338; ENSG00000104888. [Q9P2U7-2]
GeneIDi57030.
KEGGihsa:57030.
UCSCiuc002pnp.3. human. [Q9P2U7-1]

Organism-specific databases

CTDi57030.
GeneCardsiGC19M049934.
HGNCiHGNC:16704. SLC17A7.
HPAiCAB037183.
HPA050458.
HPA063679.
MIMi605208. gene.
neXtProtiNX_Q9P2U7.
PharmGKBiPA423.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ9P2U7.
KOiK12302.
OMAiPLTKFNT.
OrthoDBiEOG789C9Z.
PhylomeDBiQ9P2U7.
TreeFamiTF313535.

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19372. Organic anion transporters.

Miscellaneous databases

ChiTaRSiSLC17A7. human.
GeneWikiiVesicular_glutamate_transporter_1.
GenomeRNAii57030.
NextBioi35471565.
PROiQ9P2U7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9P2U7.
CleanExiHS_SLC17A7.
ExpressionAtlasiQ9P2U7. baseline and differential.
GenevestigatoriQ9P2U7.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel brain-type Na(+)-dependent inorganic phosphate cotransporter."
    Aihara Y., Mashima H., Onda H., Hisano S., Kasuya H., Hori T., Yamada S., Tomura H., Yamada Y., Inoue I., Kojima I., Takeda J.
    J. Neurochem. 74:2622-2625(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Amygdala.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-431.
    Tissue: Brain.

Entry informationi

Entry nameiVGLU1_HUMAN
AccessioniPrimary (citable) accession number: Q9P2U7
Secondary accession number(s): B4DFR9, B4DG46, Q6PCD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.