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Protein

GMP reductase 2

Gene

GMPR2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides (PubMed:12009299, PubMed:12669231, PubMed:16359702, PubMed:22037469). Plays a role in modulating cellular differentiation (PubMed:12669231).UniRule annotation4 Publications

Catalytic activityi

Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH.UniRule annotation3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78NADPUniRule annotation1 Publication1
Metal bindingi181Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi183Potassium; via carbonyl oxygenUniRule annotation1
Active sitei186Thioimidate intermediateUniRule annotation1
Metal bindingi186Potassium; via carbonyl oxygenUniRule annotation1
Active sitei188Proton donor/acceptorUniRule annotation1
Metal bindingi189PotassiumUniRule annotation1
Binding sitei269NADP; via carbonyl oxygenUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 27NADP; shared with neighboring subunitUniRule annotation1 Publication2
Nucleotide bindingi129 – 131NADPUniRule annotation1 Publication3
Nucleotide bindingi180 – 181NADPUniRule annotation1 Publication2
Nucleotide bindingi285 – 286NADPUniRule annotation1 Publication2
Nucleotide bindingi314 – 317NADP; shared with neighboring subunitUniRule annotation1 Publication4

GO - Molecular functioni

  • GMP reductase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

Metal-binding, NADP, Potassium

Enzyme and pathway databases

BioCyciZFISH:HS02169-MONOMER.
BRENDAi1.7.1.7. 2681.
ReactomeiR-HSA-74217. Purine salvage.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP reductase 2UniRule annotation (EC:1.7.1.7UniRule annotation)
Short name:
GMPR 2UniRule annotation
Alternative name(s):
Guanosine 5'-monophosphate oxidoreductase 2UniRule annotation
Short name:
Guanosine monophosphate reductase 2UniRule annotation
Gene namesi
Name:GMPR2UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:4377. GMPR2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi186C → A: Loss of enzyme activity. 2 Publications1
Mutagenesisi188T → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi289E → Q: Loss of enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi51292.
OpenTargetsiENSG00000100938.
PharmGKBiPA28762.

Polymorphism and mutation databases

BioMutaiGMPR2.
DMDMi25008511.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000937261 – 348GMP reductase 2Add BLAST348

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9P2T1.
MaxQBiQ9P2T1.
PaxDbiQ9P2T1.
PeptideAtlasiQ9P2T1.
PRIDEiQ9P2T1.

PTM databases

iPTMnetiQ9P2T1.
PhosphoSitePlusiQ9P2T1.

Expressioni

Tissue specificityi

Highly expressed in heart, skeletal muscle, kidney, brain, liver, prostate, spleen, placenta, testis and ovary. Low expression in colon, thymus and peripheral blood leukocytes.2 Publications

Gene expression databases

BgeeiENSG00000100938.
CleanExiHS_GMPR2.
ExpressionAtlasiQ9P2T1. baseline and differential.
GenevisibleiQ9P2T1. HS.

Organism-specific databases

HPAiHPA000904.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi119443. 7 interactors.
IntActiQ9P2T1. 2 interactors.
STRINGi9606.ENSP00000392859.

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi12 – 14Combined sources3
Beta strandi15 – 17Combined sources3
Helixi27 – 29Combined sources3
Beta strandi34 – 37Combined sources4
Turni39 – 41Combined sources3
Beta strandi44 – 47Combined sources4
Beta strandi50 – 52Combined sources3
Turni56 – 58Combined sources3
Helixi61 – 69Combined sources9
Beta strandi73 – 75Combined sources3
Helixi82 – 91Combined sources10
Helixi93 – 95Combined sources3
Beta strandi99 – 103Combined sources5
Helixi107 – 119Combined sources13
Beta strandi125 – 129Combined sources5
Helixi136 – 148Combined sources13
Beta strandi152 – 159Combined sources8
Helixi162 – 170Combined sources9
Beta strandi174 – 178Combined sources5
Helixi188 – 192Combined sources5
Helixi198 – 211Combined sources14
Beta strandi215 – 220Combined sources6
Helixi225 – 233Combined sources9
Beta strandi237 – 242Combined sources6
Turni243 – 247Combined sources5
Beta strandi248 – 251Combined sources4
Beta strandi255 – 258Combined sources4
Beta strandi261 – 267Combined sources7
Beta strandi269 – 271Combined sources3
Helixi272 – 278Combined sources7
Beta strandi279 – 281Combined sources3
Turni283 – 285Combined sources3
Beta strandi286 – 288Combined sources3
Beta strandi292 – 296Combined sources5
Helixi301 – 319Combined sources19
Helixi324 – 326Combined sources3
Helixi327 – 330Combined sources4
Beta strandi333 – 335Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A7RX-ray3.00A/B/C/D1-348[»]
2BZNX-ray2.15A/B/C/D/E/F/G/H10-341[»]
2C6QX-ray1.70A/B/C/D/E/F/G/H10-341[»]
ProteinModelPortaliQ9P2T1.
SMRiQ9P2T1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9P2T1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni219 – 221GMP bindingUniRule annotation3
Regioni242 – 243GMP bindingUniRule annotation2
Regioni268 – 270GMP bindingUniRule annotation3
Regioni286 – 290GMP bindingUniRule annotation5

Sequence similaritiesi

Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2550. Eukaryota.
COG0516. LUCA.
GeneTreeiENSGT00530000062923.
HOGENOMiHOG000165756.
HOVERGENiHBG051744.
InParanoidiQ9P2T1.
KOiK00364.
PhylomeDBiQ9P2T1.
TreeFamiTF300378.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00596. GMP_reduct_type1. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005993. GMPR.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000235. GMP_reductase. 1 hit.
TIGRFAMsiTIGR01305. GMP_reduct_1. 1 hit.
PROSITEiPS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2T1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPHIDNDVKL DFKDVLLRPK RSTLKSRSEV DLTRSFSFRN SKQTYSGVPI
60 70 80 90 100
IAANMDTVGT FEMAKVLCKF SLFTAVHKHY SLVQWQEFAG QNPDCLEHLA
110 120 130 140 150
ASSGTGSSDF EQLEQILEAI PQVKYICLDV ANGYSEHFVE FVKDVRKRFP
160 170 180 190 200
QHTIMAGNVV TGEMVEELIL SGADIIKVGI GPGSVCTTRK KTGVGYPQLS
210 220 230 240 250
AVMECADAAH GLKGHIISDG GCSCPGDVAK AFGAGADFVM LGGMLAGHSE
260 270 280 290 300
SGGELIERDG KKYKLFYGMS SEMAMKKYAG GVAEYRASEG KTVEVPFKGD
310 320 330 340
VEHTIRDILG GIRSTCTYVG AAKLKELSRR TTFIRVTQQV NPIFSEAC
Length:348
Mass (Da):37,874
Last modified:October 1, 2000 - v1
Checksum:iE7812A754C433E51
GO
Isoform 2 (identifier: Q9P2T1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTSCLPALRFIATPRLSAM

Show »
Length:366
Mass (Da):39,805
Checksum:i009CF162967F2112
GO
Isoform 3 (identifier: Q9P2T1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-97: Missing.

Note: No experimental confirmation available.
Show »
Length:320
Mass (Da):34,597
Checksum:iA54872F5C9635EAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91Q → R in CAG33437 (Ref. 5) Curated1
Sequence conflicti200S → G in CAG33437 (Ref. 5) Curated1
Sequence conflicti223S → N in CAG33437 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049602242G → D.Corresponds to variant rs34354104dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0414591M → MTSCLPALRFIATPRLSAM in isoform 2. 1 Publication1
Alternative sequenceiVSP_05458570 – 97Missing in isoform 3. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419346 mRNA. Translation: AAN32701.1.
AF135159 mRNA. Translation: AAG09132.1.
AB032903 mRNA. Translation: BAA93080.1.
BX161436 mRNA. Translation: CAD61908.1.
BX247993 mRNA. Translation: CAD62327.1.
CR457156 mRNA. Translation: CAG33437.1.
AL096870 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW66051.1.
CH471078 Genomic DNA. Translation: EAW66053.1.
CH471078 Genomic DNA. Translation: EAW66054.1.
CH471078 Genomic DNA. Translation: EAW66056.1.
CH471078 Genomic DNA. Translation: EAW66057.1.
BC008021 mRNA. Translation: AAH08021.2.
BC009832 mRNA. Translation: AAH09832.1.
BC093039 mRNA. Translation: AAH93039.1.
CCDSiCCDS41935.1. [Q9P2T1-1]
CCDS45087.1. [Q9P2T1-2]
CCDS61419.1. [Q9P2T1-3]
RefSeqiNP_001002000.1. NM_001002000.2. [Q9P2T1-1]
NP_001002001.1. NM_001002001.2. [Q9P2T1-1]
NP_001002002.1. NM_001002002.2. [Q9P2T1-1]
NP_001269950.1. NM_001283021.1.
NP_001269951.1. NM_001283022.1.
NP_001269952.1. NM_001283023.1. [Q9P2T1-3]
NP_057660.2. NM_016576.4. [Q9P2T1-2]
UniGeneiHs.368855.

Genome annotation databases

EnsembliENST00000355299; ENSP00000347449; ENSG00000100938. [Q9P2T1-1]
ENST00000399440; ENSP00000382369; ENSG00000100938. [Q9P2T1-1]
ENST00000420554; ENSP00000392859; ENSG00000100938. [Q9P2T1-2]
ENST00000456667; ENSP00000405743; ENSG00000100938. [Q9P2T1-3]
ENST00000559836; ENSP00000453299; ENSG00000100938. [Q9P2T1-1]
GeneIDi51292.
KEGGihsa:51292.
UCSCiuc001wnr.5. human. [Q9P2T1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF419346 mRNA. Translation: AAN32701.1.
AF135159 mRNA. Translation: AAG09132.1.
AB032903 mRNA. Translation: BAA93080.1.
BX161436 mRNA. Translation: CAD61908.1.
BX247993 mRNA. Translation: CAD62327.1.
CR457156 mRNA. Translation: CAG33437.1.
AL096870 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW66051.1.
CH471078 Genomic DNA. Translation: EAW66053.1.
CH471078 Genomic DNA. Translation: EAW66054.1.
CH471078 Genomic DNA. Translation: EAW66056.1.
CH471078 Genomic DNA. Translation: EAW66057.1.
BC008021 mRNA. Translation: AAH08021.2.
BC009832 mRNA. Translation: AAH09832.1.
BC093039 mRNA. Translation: AAH93039.1.
CCDSiCCDS41935.1. [Q9P2T1-1]
CCDS45087.1. [Q9P2T1-2]
CCDS61419.1. [Q9P2T1-3]
RefSeqiNP_001002000.1. NM_001002000.2. [Q9P2T1-1]
NP_001002001.1. NM_001002001.2. [Q9P2T1-1]
NP_001002002.1. NM_001002002.2. [Q9P2T1-1]
NP_001269950.1. NM_001283021.1.
NP_001269951.1. NM_001283022.1.
NP_001269952.1. NM_001283023.1. [Q9P2T1-3]
NP_057660.2. NM_016576.4. [Q9P2T1-2]
UniGeneiHs.368855.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A7RX-ray3.00A/B/C/D1-348[»]
2BZNX-ray2.15A/B/C/D/E/F/G/H10-341[»]
2C6QX-ray1.70A/B/C/D/E/F/G/H10-341[»]
ProteinModelPortaliQ9P2T1.
SMRiQ9P2T1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119443. 7 interactors.
IntActiQ9P2T1. 2 interactors.
STRINGi9606.ENSP00000392859.

PTM databases

iPTMnetiQ9P2T1.
PhosphoSitePlusiQ9P2T1.

Polymorphism and mutation databases

BioMutaiGMPR2.
DMDMi25008511.

Proteomic databases

EPDiQ9P2T1.
MaxQBiQ9P2T1.
PaxDbiQ9P2T1.
PeptideAtlasiQ9P2T1.
PRIDEiQ9P2T1.

Protocols and materials databases

DNASUi51292.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355299; ENSP00000347449; ENSG00000100938. [Q9P2T1-1]
ENST00000399440; ENSP00000382369; ENSG00000100938. [Q9P2T1-1]
ENST00000420554; ENSP00000392859; ENSG00000100938. [Q9P2T1-2]
ENST00000456667; ENSP00000405743; ENSG00000100938. [Q9P2T1-3]
ENST00000559836; ENSP00000453299; ENSG00000100938. [Q9P2T1-1]
GeneIDi51292.
KEGGihsa:51292.
UCSCiuc001wnr.5. human. [Q9P2T1-1]

Organism-specific databases

CTDi51292.
DisGeNETi51292.
GeneCardsiGMPR2.
HGNCiHGNC:4377. GMPR2.
HPAiHPA000904.
MIMi610781. gene.
neXtProtiNX_Q9P2T1.
OpenTargetsiENSG00000100938.
PharmGKBiPA28762.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2550. Eukaryota.
COG0516. LUCA.
GeneTreeiENSGT00530000062923.
HOGENOMiHOG000165756.
HOVERGENiHBG051744.
InParanoidiQ9P2T1.
KOiK00364.
PhylomeDBiQ9P2T1.
TreeFamiTF300378.

Enzyme and pathway databases

BioCyciZFISH:HS02169-MONOMER.
BRENDAi1.7.1.7. 2681.
ReactomeiR-HSA-74217. Purine salvage.

Miscellaneous databases

ChiTaRSiGMPR2. human.
EvolutionaryTraceiQ9P2T1.
GenomeRNAii51292.
PROiQ9P2T1.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100938.
CleanExiHS_GMPR2.
ExpressionAtlasiQ9P2T1. baseline and differential.
GenevisibleiQ9P2T1. HS.

Family and domain databases

CDDicd00381. IMPDH. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00596. GMP_reduct_type1. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005993. GMPR.
IPR015875. IMP_DH/GMP_Rdtase_CS.
IPR001093. IMP_DH_GMPRt.
[Graphical view]
PfamiPF00478. IMPDH. 1 hit.
[Graphical view]
PIRSFiPIRSF000235. GMP_reductase. 1 hit.
TIGRFAMsiTIGR01305. GMP_reduct_1. 1 hit.
PROSITEiPS00487. IMP_DH_GMP_RED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGMPR2_HUMAN
AccessioniPrimary (citable) accession number: Q9P2T1
Secondary accession number(s): D3DS66
, Q567T0, Q6IAJ8, Q86T14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.