Reviewed, UniProtKB/Swiss-Prot Q9P2S5 (WDR8_HUMAN)

Names and origin

Protein names

Recommended name:
    WD repeat-containing protein 8

Gene names

Name:

WDR8

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm Potential.
Tissue specificity	Ubiquitous. Predominant expression in heart, brain, liver, thymus, prostate, and testis, and barely detectable expression in lung.
Sequence similarities	Contains 6 WD repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q65ZQ1	1	EBI-1054904,EBI-1054063
ARMC6	Q6NXE6	1	EBI-1054904,EBI-1053802
ATP2A3	Q93084	1	EBI-1054904,EBI-1046185
BAT3	P46379	1	EBI-1054904,EBI-347552
C7orf20	Q7L5D6	1	EBI-1054904,EBI-711823
CDK2	P24941	1	EBI-1054904,EBI-375096
CEP135	Q66GS9	1	EBI-1054904,EBI-1046993
COPS2	P61201	1	EBI-1054904,EBI-1050386
COPS3	Q9UNS2	1	EBI-1054904,EBI-350590
COPS4	Q9BT78	1	EBI-1054904,EBI-742413
GK006	Q6P2I7	1	EBI-1054904,EBI-1047027
GPS1	Q13098	1	EBI-1054904,EBI-725197
KIAA1967	Q8N163	1	EBI-1054904,EBI-355410
LRPPRC	P42704	1	EBI-1054904,EBI-1050853
LYZ	P61626	1	EBI-1054904,EBI-355360
MCM3	P25205	1	EBI-1054904,EBI-355153
MTHFD1	P11586	1	EBI-1054904,EBI-709638
MTHFD1L	Q6UB35	1	EBI-1054904,EBI-1046835
MYO1C	O00159	1	EBI-1054904,EBI-350423
OGT	O15294	1	EBI-1054904,EBI-539828
PFDN1	O60925	1	EBI-1054904,EBI-356919
PFDN2	Q9UHV9	1	EBI-1054904,EBI-359873
PFDN6	O15212	1	EBI-1054904,EBI-356973
PHYH	O14832	1	EBI-1054904,EBI-721853
PSMD2	Q13200	1	EBI-1054904,EBI-357648
PSMD3	O43242	1	EBI-1054904,EBI-357622
SSR4	P51571	1	EBI-1054904,EBI-1054539
STIP1	P31948	1	EBI-1054904,EBI-1054052
UNC45A	Q9H3U1	1	EBI-1054904,EBI-1048763
VCP	Q5VYM0	1	EBI-1054904,EBI-1046421

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P2S5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P2S5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     415-460: DFAVLSLCWHLSGDSMALLSKDHFCLCFLETEAVVGTACRQLGGHT → KHIPEATDVAALLCAVWHHTG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	WD repeat-containing protein 8		PRO_0000051356
Regions
	Repeat	46 – 86	41	WD 1
	Repeat	89 – 129	41	WD 2
	Repeat	176 – 210	35	WD 3
	Repeat	221 – 260	40	WD 4
	Repeat	328 – 369	42	WD 5
	Repeat	371 – 410	40	WD 6
Natural variations
	Alternative sequence	415 – 460	46	DFAVL…LGGHT → KHIPEATDVAALLCAVWHHT G in isoform 2.		VSP_016112
	Natural variant	331	1	I → M: dbSNP rs2760320.		VAR_057624
	Natural variant	358	1	V → I: dbSNP rs16823940.		VAR_057625
Experimental info
	Sequence conflict	381	1	W → R in BAA91164. Ref.2
	Sequence conflict	405	1	C → R in BAA91164. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C2E318B1F06F79BF	FASTA	460	51,588
	10 20 30 40 50 60 MNFSEVFKLS SLLCKFSPDG KYLASCVQYR LVVRDVNTLQ ILQLYTCLDQ IQHIEWSADS 70 80 90 100 110 120 LFILCAMYKR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS 130 140 150 160 170 180 LCTKSVSYIK YPKACLQGIT FTRDGRYMAL AERRDCKDYV SIFVCSDWQL LRHFDTDTQD 190 200 210 220 230 240 LTGIEWAPNG CVLAVWDTCL EYKILLYSLD GRLLSTYSAY EWSLGIKSVA WSPSSQFLAV 250 260 270 280 290 300 GSYDGKVRIL NHVTWKMITE FGHPAAINDP KIVVYKEAEK SPQLGLGCLS FPPPRAGAGP 310 320 330 340 350 360 LPSSESKYEI ASVPVSLQTL KPVTDRANPK IGIGMLAFSP DSYFLATRND NIPNAVWVWD 370 380 390 400 410 420 IQKLRLFAVL EQLSPVRAFQ WDPQQPRLAI CTGGSRLYLW SPAGCMSVQV PGEGDFAVLS 430 440 450 460 LCWHLSGDSM ALLSKDHFCL CFLETEAVVG TACRQLGGHT
	Isoform 2. Checksum: D8EB07145FCD6500	FASTA	435	48,874
	10 20 30 40 50 60 MNFSEVFKLS SLLCKFSPDG KYLASCVQYR LVVRDVNTLQ ILQLYTCLDQ IQHIEWSADS 70 80 90 100 110 120 LFILCAMYKR GLVQVWSLEQ PEWHCKIDEG SAGLVASCWS PDGRHILNTT EFHLRITVWS 130 140 150 160 170 180 LCTKSVSYIK YPKACLQGIT FTRDGRYMAL AERRDCKDYV SIFVCSDWQL LRHFDTDTQD 190 200 210 220 230 240 LTGIEWAPNG CVLAVWDTCL EYKILLYSLD GRLLSTYSAY EWSLGIKSVA WSPSSQFLAV 250 260 270 280 290 300 GSYDGKVRIL NHVTWKMITE FGHPAAINDP KIVVYKEAEK SPQLGLGCLS FPPPRAGAGP 310 320 330 340 350 360 LPSSESKYEI ASVPVSLQTL KPVTDRANPK IGIGMLAFSP DSYFLATRND NIPNAVWVWD 370 380 390 400 410 420 IQKLRLFAVL EQLSPVRAFQ WDPQQPRLAI CTGGSRLYLW SPAGCMSVQV PGEGKHIPEA 430 TDVAALLCAV WHHTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization, and mapping of the mouse and human WDR8 genes, members of a novel WD-repeat gene family." Koshizuka Y., Ikegawa S., Sano M., Nakamura K., Nakamura Y. Genomics 72:252-259(2001) [PubMed: 11401440] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Carcinoma.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lung and Lymph.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB034912 mRNA. Translation: BAA92312.1. AK000437 mRNA. Translation: BAA91164.1. AL513320, AL136528 Genomic DNA. Translation: CAI14335.1. AL513320, AL136528 Genomic DNA. Translation: CAI14336.1. AL136528, AL513320 Genomic DNA. Translation: CAI19128.1. AL136528, AL513320 Genomic DNA. Translation: CAI19129.1. BC002611 mRNA. Translation: AAH02611.1. BC086311 mRNA. Translation: AAH86311.1.
IPI	IPI00293015. IPI00644542.
RefSeq	NP_060288.3.
UniGene	Hs.31714
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P2S5. 57 interactions.
PTM databases
PhosphoSite	Q9P2S5.
Proteomic databases
PRIDE	Q9P2S5.
Genome annotation databases
Ensembl	ENSG00000116213. Homo sapiens. [Contig view]
GeneID	49856.
KEGG	hsa:49856.
Organism-specific databases
GeneCards	GC01M003570.
H-InvDB	HIX0020054.
HGNC	HGNC:12759. WDR8.
MIM	606040. gene.
PharmGKB	PA37363.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9P2S5.
HOVERGEN	Q9P2S5.
OMA	Q9P2S5. ERRDCKD.
Gene expression databases
ArrayExpress	Q9P2S5.
Bgee	Q9P2S5.
CleanEx	HS_WDR8.
GermOnline	ENSG00000116213. Homo sapiens.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. IPR019775. WD40_repeat_CS. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 2 hits.
Pfam	PF00400. WD40. 1 hit. [Graphical view]
SMART	SM00320. WD40. 4 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	53024.
SOURCE	Search...

Entry information

Entry name

WDR8_HUMAN

Accession

Primary (citable) accession number: Q9P2S5
Secondary accession number(s): Q5T0D6 , Q9BUH7, Q9NTK7, Q9NX56

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)