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Q9P2P5

- HECW2_HUMAN

UniProt

Q9P2P5 - HECW2_HUMAN

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Protein
E3 ubiquitin-protein ligase HECW2
Gene
HECW2, KIAA1301, NEDL2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1540 – 15401Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: RefGenome

GO - Biological processi

  1. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

SignaLinkiQ9P2P5.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HECW2 (EC:6.3.2.-)
Alternative name(s):
HECT, C2 and WW domain-containing protein 2
NEDD4-like E3 ubiquitin-protein ligase 2
Gene namesi
Name:HECW2
Synonyms:KIAA1301, NEDL2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:29853. HECW2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: RefGenome
  2. nucleus Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134925001.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15721572E3 ubiquitin-protein ligase HECW2
PRO_0000277667Add
BLAST

Proteomic databases

MaxQBiQ9P2P5.
PaxDbiQ9P2P5.
PeptideAtlasiQ9P2P5.
PRIDEiQ9P2P5.

PTM databases

PhosphoSiteiQ9P2P5.

Expressioni

Tissue specificityi

Predominantly expressed in adult brain, lung and heart.1 Publication

Gene expression databases

ArrayExpressiQ9P2P5.
BgeeiQ9P2P5.
CleanExiHS_HECW2.
GenevestigatoriQ9P2P5.

Organism-specific databases

HPAiHPA034609.
HPA044858.

Interactioni

Subunit structurei

Interacts with TP73.1 Publication

Protein-protein interaction databases

BioGridi121581. 284 interactions.
IntActiQ9P2P5. 1 interaction.
MINTiMINT-2820726.
STRINGi9606.ENSP00000260983.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi60 – 645
Beta strandi66 – 694
Beta strandi76 – 816
Beta strandi91 – 966
Beta strandi117 – 1237
Beta strandi133 – 14412
Turni145 – 1484
Beta strandi149 – 1535
Beta strandi157 – 1604

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LFENMR-A43-162[»]
ProteinModelPortaliQ9P2P5.
SMRiQ9P2P5. Positions 57-162, 191-305, 806-898, 915-1022, 1168-1569.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 282111C2
Add
BLAST
Domaini807 – 84034WW 1
Add
BLAST
Domaini985 – 101834WW 2
Add
BLAST
Domaini1237 – 1572336HECT
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni737 – 1068332Interaction with TP73
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili847 – 87428 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.
Contains 2 WW domains.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG5021.
HOGENOMiHOG000069940.
HOVERGENiHBG057414.
InParanoidiQ9P2P5.
KOiK12168.
OMAiTVCSNGP.
OrthoDBiEOG7BKCTS.
PhylomeDBiQ9P2P5.
TreeFamiTF313938.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF00397. WW. 2 hits.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 2 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9P2P5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASSAREHLL FVRRRNPQMR YTLSPENLQS LAAQSSMPEN MTLQRANSDT     50
DLVTSESRSS LTASMYEYTL GQAQNLIIFW DIKEEVDPSD WIGLYHIDEN 100
SPANFWDSKN RGVTGTQKGQ IVWRIEPGPY FMEPEIKICF KYYHGISGAL 150
RATTPCITVK NPAVMMGAEG MEGGASGNLH SRKLVSFTLS DLRAVGLKKG 200
MFFNPDPYLK MSIQPGKKSS FPTCAHHGQE RRSTIISNTT NPIWHREKYS 250
FFALLTDVLE IEIKDKFAKS RPIIKRFLGK LTIPVQRLLE RQAIGDQMLS 300
YNLGRRLPAD HVSGYLQFKV EVTSSVHEDA SPEAVGTILG VNSVNGDLGS 350
PSDDEDMPGS HHDSQVCSNG PVSEDSAADG TPKHSFRTSS TLEIDTEELT 400
STSSRTSPPR GRQDSLNDYL DAIEHNGHSR PGTATCSERS MGASPKLRSS 450
FPTDTRLNAM LHIDSDEEDH EFQQDLGYPS SLEEEGGLIM FSRASRADDG 500
SLTSQTKLED NPVENEEAST HEAASFEDKP ENLPELAESS LPAGPAPEEG 550
EGGPEPQPSA DQGSAELCGS QEVDQPTSGA DTGTSDASGG SRRAVSETES 600
LDQGSEPSQV SSETEPSDPA RTESVSEAST RPEGESDLEC ADSSCNESVT 650
TQLSSVDTRC SSLESARFPE TPAFSSQEEE DGACAAEPTS SGPAEGSQES 700
VCTAGSLPVV QVPSGEDEGP GAESATVPDQ EELGEVWQRR GSLEGAAAAA 750
ESPPQEEGSA GEAQGTCEGA TAQEEGATGG SQANGHQPLR SLPSVRQDVS 800
RYQRVDEALP PNWEARIDSH GRIFYVDHVN RTTTWQRPTA PPAPQVLQRS 850
NSIQQMEQLN RRYQSIRRTM TNERPEENTN AIDGAGEEAD FHQASADFRR 900
ENILPHSTSR SRITLLLQSP PVKFLISPEF FTVLHSNPSA YRMFTNNTCL 950
KHMITKVRRD THHFERYQHN RDLVGFLNMF ANKQLELPRG WEMKHDHQGK 1000
AFFVDHNSRT TTFIDPRLPL QSSRPTSALV HRQHLTRQRS HSAGEVGEDS 1050
RHAGPPVLPR PSSTFNTVSR PQYQDMVPVA YNDKIVAFLR QPNIFEILQE 1100
RQPDLTRNHS LREKIQFIRT EGTPGLVRLS SDADLVMLLS LFEEEIMSYV 1150
PPHALLHPSY CQSPRGSPVS SPQNSPGTQR ANARAPAPYK RDFEAKLRNF 1200
YRKLETKGYG QGPGKLKLII RRDHLLEDAF NQIMGYSRKD LQRNKLYVTF 1250
VGEEGLDYSG PSREFFFLVS RELFNPYYGL FEYSANDTYT VQISPMSAFV 1300
DNHHEWFRFS GRILGLALIH QYLLDAFFTR PFYKALLRIL CDLSDLEYLD 1350
EEFHQSLQWM KDNDIHDILD LTFTVNEEVF GQITERELKP GGANIPVTEK 1400
NKKEYIERMV KWRIERGVVQ QTESLVRGFY EVVDARLVSV FDARELELVI 1450
AGTAEIDLSD WRNNTEYRGG YHDNHIVIRW FWAAVERFNN EQRLRLLQFV 1500
TGTSSIPYEG FASLRGSNGP RRFCVEKWGK ITALPRAHTC FNRLDLPPYP 1550
SFSMLYEKLL TAVEETSTFG LE 1572
Length:1,572
Mass (Da):175,769
Last modified:February 20, 2007 - v2
Checksum:iDCFF0146ADFAF8BE
GO
Isoform 2 (identifier: Q9P2P5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-356: Missing.

Note: No experimental confirmation available.

Show »
Length:1,216
Mass (Da):135,905
Checksum:i51B974BF1F83D6D1
GO

Sequence cautioni

The sequence BAA92539.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 356356Missing in isoform 2.
VSP_023077Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1074 – 10741Q → H in CAH18262. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB037722 mRNA. Translation: BAA92539.1. Different initiation.
AL390186 mRNA. Translation: CAB99103.1.
CR749424 mRNA. Translation: CAH18262.1.
AC020571 Genomic DNA. No translation available.
AC068544 Genomic DNA. No translation available.
AC073905 Genomic DNA. No translation available.
AC074090 Genomic DNA. No translation available.
AC093379 Genomic DNA. No translation available.
BC117194 mRNA. Translation: AAI17195.1.
BC117198 mRNA. Translation: AAI17199.1.
CCDSiCCDS33354.1. [Q9P2P5-1]
PIRiT51886.
RefSeqiNP_065811.1. NM_020760.1. [Q9P2P5-1]
UniGeneiHs.654742.

Genome annotation databases

EnsembliENST00000260983; ENSP00000260983; ENSG00000138411. [Q9P2P5-1]
ENST00000409111; ENSP00000386775; ENSG00000138411. [Q9P2P5-2]
GeneIDi57520.
KEGGihsa:57520.
UCSCiuc002utl.1. human. [Q9P2P5-1]

Polymorphism databases

DMDMi126215718.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB037722 mRNA. Translation: BAA92539.1 . Different initiation.
AL390186 mRNA. Translation: CAB99103.1 .
CR749424 mRNA. Translation: CAH18262.1 .
AC020571 Genomic DNA. No translation available.
AC068544 Genomic DNA. No translation available.
AC073905 Genomic DNA. No translation available.
AC074090 Genomic DNA. No translation available.
AC093379 Genomic DNA. No translation available.
BC117194 mRNA. Translation: AAI17195.1 .
BC117198 mRNA. Translation: AAI17199.1 .
CCDSi CCDS33354.1. [Q9P2P5-1 ]
PIRi T51886.
RefSeqi NP_065811.1. NM_020760.1. [Q9P2P5-1 ]
UniGenei Hs.654742.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LFE NMR - A 43-162 [» ]
ProteinModelPortali Q9P2P5.
SMRi Q9P2P5. Positions 57-162, 191-305, 806-898, 915-1022, 1168-1569.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121581. 284 interactions.
IntActi Q9P2P5. 1 interaction.
MINTi MINT-2820726.
STRINGi 9606.ENSP00000260983.

PTM databases

PhosphoSitei Q9P2P5.

Polymorphism databases

DMDMi 126215718.

Proteomic databases

MaxQBi Q9P2P5.
PaxDbi Q9P2P5.
PeptideAtlasi Q9P2P5.
PRIDEi Q9P2P5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000260983 ; ENSP00000260983 ; ENSG00000138411 . [Q9P2P5-1 ]
ENST00000409111 ; ENSP00000386775 ; ENSG00000138411 . [Q9P2P5-2 ]
GeneIDi 57520.
KEGGi hsa:57520.
UCSCi uc002utl.1. human. [Q9P2P5-1 ]

Organism-specific databases

CTDi 57520.
GeneCardsi GC02M197064.
HGNCi HGNC:29853. HECW2.
HPAi HPA034609.
HPA044858.
neXtProti NX_Q9P2P5.
PharmGKBi PA134925001.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5021.
HOGENOMi HOG000069940.
HOVERGENi HBG057414.
InParanoidi Q9P2P5.
KOi K12168.
OMAi TVCSNGP.
OrthoDBi EOG7BKCTS.
PhylomeDBi Q9P2P5.
TreeFami TF313938.

Enzyme and pathway databases

UniPathwayi UPA00143 .
SignaLinki Q9P2P5.

Miscellaneous databases

ChiTaRSi HECW2. human.
GenomeRNAii 57520.
NextBioi 63895.
PROi Q9P2P5.

Gene expression databases

ArrayExpressi Q9P2P5.
Bgeei Q9P2P5.
CleanExi HS_HECW2.
Genevestigatori Q9P2P5.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR000008. C2_dom.
IPR000569. HECT.
IPR001202. WW_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF00397. WW. 2 hits.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 2 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 2 hits.
SSF56204. SSF56204. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1352-1572 (ISOFORM 1).
    Tissue: Uterus.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Heart and Lung.
  5. "A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity."
    Miyazaki K., Ozaki T., Kato C., Hanamoto T., Fujita T., Irino S., Watanabe K., Nakagawa T., Nakagawara A.
    Biochem. Biophys. Res. Commun. 308:106-113(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TP73, TISSUE SPECIFICITY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHECW2_HUMAN
AccessioniPrimary (citable) accession number: Q9P2P5
Secondary accession number(s): B8ZZB4
, Q17RT5, Q68DF8, Q9NPS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: September 3, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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