Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9P2P5

- HECW2_HUMAN

UniProt

Q9P2P5 - HECW2_HUMAN

Protein

E3 ubiquitin-protein ligase HECW2

Gene

HECW2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (20 Feb 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei1540 – 15401Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: RefGenome

    GO - Biological processi

    1. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: RefGenome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    SignaLinkiQ9P2P5.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase HECW2 (EC:6.3.2.-)
    Alternative name(s):
    HECT, C2 and WW domain-containing protein 2
    NEDD4-like E3 ubiquitin-protein ligase 2
    Gene namesi
    Name:HECW2
    Synonyms:KIAA1301, NEDL2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:29853. HECW2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134925001.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15721572E3 ubiquitin-protein ligase HECW2PRO_0000277667Add
    BLAST

    Proteomic databases

    MaxQBiQ9P2P5.
    PaxDbiQ9P2P5.
    PeptideAtlasiQ9P2P5.
    PRIDEiQ9P2P5.

    PTM databases

    PhosphoSiteiQ9P2P5.

    Expressioni

    Tissue specificityi

    Predominantly expressed in adult brain, lung and heart.1 Publication

    Gene expression databases

    ArrayExpressiQ9P2P5.
    BgeeiQ9P2P5.
    CleanExiHS_HECW2.
    GenevestigatoriQ9P2P5.

    Organism-specific databases

    HPAiHPA034609.
    HPA044858.

    Interactioni

    Subunit structurei

    Interacts with TP73.1 Publication

    Protein-protein interaction databases

    BioGridi121581. 284 interactions.
    IntActiQ9P2P5. 1 interaction.
    MINTiMINT-2820726.
    STRINGi9606.ENSP00000260983.

    Structurei

    Secondary structure

    1
    1572
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi60 – 645
    Beta strandi66 – 694
    Beta strandi76 – 816
    Beta strandi91 – 966
    Beta strandi117 – 1237
    Beta strandi133 – 14412
    Turni145 – 1484
    Beta strandi149 – 1535
    Beta strandi157 – 1604

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LFENMR-A43-162[»]
    ProteinModelPortaliQ9P2P5.
    SMRiQ9P2P5. Positions 57-162, 191-305, 806-898, 915-1022, 1168-1569.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini172 – 282111C2PROSITE-ProRule annotationAdd
    BLAST
    Domaini807 – 84034WW 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini985 – 101834WW 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1237 – 1572336HECTPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni737 – 1068332Interaction with TP73Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili847 – 87428Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
    Contains 2 WW domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiCOG5021.
    HOGENOMiHOG000069940.
    HOVERGENiHBG057414.
    InParanoidiQ9P2P5.
    KOiK12168.
    OMAiTVCSNGP.
    OrthoDBiEOG7BKCTS.
    PhylomeDBiQ9P2P5.
    TreeFamiTF313938.

    Family and domain databases

    Gene3Di2.60.40.150. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR000569. HECT.
    IPR001202. WW_dom.
    [Graphical view]
    PfamiPF00168. C2. 1 hit.
    PF00632. HECT. 1 hit.
    PF00397. WW. 2 hits.
    [Graphical view]
    SMARTiSM00239. C2. 1 hit.
    SM00119. HECTc. 1 hit.
    SM00456. WW. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51045. SSF51045. 2 hits.
    SSF56204. SSF56204. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50237. HECT. 1 hit.
    PS01159. WW_DOMAIN_1. 2 hits.
    PS50020. WW_DOMAIN_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9P2P5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASSAREHLL FVRRRNPQMR YTLSPENLQS LAAQSSMPEN MTLQRANSDT     50
    DLVTSESRSS LTASMYEYTL GQAQNLIIFW DIKEEVDPSD WIGLYHIDEN 100
    SPANFWDSKN RGVTGTQKGQ IVWRIEPGPY FMEPEIKICF KYYHGISGAL 150
    RATTPCITVK NPAVMMGAEG MEGGASGNLH SRKLVSFTLS DLRAVGLKKG 200
    MFFNPDPYLK MSIQPGKKSS FPTCAHHGQE RRSTIISNTT NPIWHREKYS 250
    FFALLTDVLE IEIKDKFAKS RPIIKRFLGK LTIPVQRLLE RQAIGDQMLS 300
    YNLGRRLPAD HVSGYLQFKV EVTSSVHEDA SPEAVGTILG VNSVNGDLGS 350
    PSDDEDMPGS HHDSQVCSNG PVSEDSAADG TPKHSFRTSS TLEIDTEELT 400
    STSSRTSPPR GRQDSLNDYL DAIEHNGHSR PGTATCSERS MGASPKLRSS 450
    FPTDTRLNAM LHIDSDEEDH EFQQDLGYPS SLEEEGGLIM FSRASRADDG 500
    SLTSQTKLED NPVENEEAST HEAASFEDKP ENLPELAESS LPAGPAPEEG 550
    EGGPEPQPSA DQGSAELCGS QEVDQPTSGA DTGTSDASGG SRRAVSETES 600
    LDQGSEPSQV SSETEPSDPA RTESVSEAST RPEGESDLEC ADSSCNESVT 650
    TQLSSVDTRC SSLESARFPE TPAFSSQEEE DGACAAEPTS SGPAEGSQES 700
    VCTAGSLPVV QVPSGEDEGP GAESATVPDQ EELGEVWQRR GSLEGAAAAA 750
    ESPPQEEGSA GEAQGTCEGA TAQEEGATGG SQANGHQPLR SLPSVRQDVS 800
    RYQRVDEALP PNWEARIDSH GRIFYVDHVN RTTTWQRPTA PPAPQVLQRS 850
    NSIQQMEQLN RRYQSIRRTM TNERPEENTN AIDGAGEEAD FHQASADFRR 900
    ENILPHSTSR SRITLLLQSP PVKFLISPEF FTVLHSNPSA YRMFTNNTCL 950
    KHMITKVRRD THHFERYQHN RDLVGFLNMF ANKQLELPRG WEMKHDHQGK 1000
    AFFVDHNSRT TTFIDPRLPL QSSRPTSALV HRQHLTRQRS HSAGEVGEDS 1050
    RHAGPPVLPR PSSTFNTVSR PQYQDMVPVA YNDKIVAFLR QPNIFEILQE 1100
    RQPDLTRNHS LREKIQFIRT EGTPGLVRLS SDADLVMLLS LFEEEIMSYV 1150
    PPHALLHPSY CQSPRGSPVS SPQNSPGTQR ANARAPAPYK RDFEAKLRNF 1200
    YRKLETKGYG QGPGKLKLII RRDHLLEDAF NQIMGYSRKD LQRNKLYVTF 1250
    VGEEGLDYSG PSREFFFLVS RELFNPYYGL FEYSANDTYT VQISPMSAFV 1300
    DNHHEWFRFS GRILGLALIH QYLLDAFFTR PFYKALLRIL CDLSDLEYLD 1350
    EEFHQSLQWM KDNDIHDILD LTFTVNEEVF GQITERELKP GGANIPVTEK 1400
    NKKEYIERMV KWRIERGVVQ QTESLVRGFY EVVDARLVSV FDARELELVI 1450
    AGTAEIDLSD WRNNTEYRGG YHDNHIVIRW FWAAVERFNN EQRLRLLQFV 1500
    TGTSSIPYEG FASLRGSNGP RRFCVEKWGK ITALPRAHTC FNRLDLPPYP 1550
    SFSMLYEKLL TAVEETSTFG LE 1572
    Length:1,572
    Mass (Da):175,769
    Last modified:February 20, 2007 - v2
    Checksum:iDCFF0146ADFAF8BE
    GO
    Isoform 2 (identifier: Q9P2P5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-356: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,216
    Mass (Da):135,905
    Checksum:i51B974BF1F83D6D1
    GO

    Sequence cautioni

    The sequence BAA92539.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1074 – 10741Q → H in CAH18262. (PubMed:17974005)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 356356Missing in isoform 2. 1 PublicationVSP_023077Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB037722 mRNA. Translation: BAA92539.1. Different initiation.
    AL390186 mRNA. Translation: CAB99103.1.
    CR749424 mRNA. Translation: CAH18262.1.
    AC020571 Genomic DNA. No translation available.
    AC068544 Genomic DNA. No translation available.
    AC073905 Genomic DNA. No translation available.
    AC074090 Genomic DNA. No translation available.
    AC093379 Genomic DNA. No translation available.
    BC117194 mRNA. Translation: AAI17195.1.
    BC117198 mRNA. Translation: AAI17199.1.
    CCDSiCCDS33354.1. [Q9P2P5-1]
    PIRiT51886.
    RefSeqiNP_065811.1. NM_020760.1. [Q9P2P5-1]
    UniGeneiHs.654742.

    Genome annotation databases

    EnsembliENST00000260983; ENSP00000260983; ENSG00000138411. [Q9P2P5-1]
    ENST00000409111; ENSP00000386775; ENSG00000138411. [Q9P2P5-2]
    GeneIDi57520.
    KEGGihsa:57520.
    UCSCiuc002utl.1. human. [Q9P2P5-1]

    Polymorphism databases

    DMDMi126215718.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB037722 mRNA. Translation: BAA92539.1 . Different initiation.
    AL390186 mRNA. Translation: CAB99103.1 .
    CR749424 mRNA. Translation: CAH18262.1 .
    AC020571 Genomic DNA. No translation available.
    AC068544 Genomic DNA. No translation available.
    AC073905 Genomic DNA. No translation available.
    AC074090 Genomic DNA. No translation available.
    AC093379 Genomic DNA. No translation available.
    BC117194 mRNA. Translation: AAI17195.1 .
    BC117198 mRNA. Translation: AAI17199.1 .
    CCDSi CCDS33354.1. [Q9P2P5-1 ]
    PIRi T51886.
    RefSeqi NP_065811.1. NM_020760.1. [Q9P2P5-1 ]
    UniGenei Hs.654742.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LFE NMR - A 43-162 [» ]
    ProteinModelPortali Q9P2P5.
    SMRi Q9P2P5. Positions 57-162, 191-305, 806-898, 915-1022, 1168-1569.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121581. 284 interactions.
    IntActi Q9P2P5. 1 interaction.
    MINTi MINT-2820726.
    STRINGi 9606.ENSP00000260983.

    PTM databases

    PhosphoSitei Q9P2P5.

    Polymorphism databases

    DMDMi 126215718.

    Proteomic databases

    MaxQBi Q9P2P5.
    PaxDbi Q9P2P5.
    PeptideAtlasi Q9P2P5.
    PRIDEi Q9P2P5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000260983 ; ENSP00000260983 ; ENSG00000138411 . [Q9P2P5-1 ]
    ENST00000409111 ; ENSP00000386775 ; ENSG00000138411 . [Q9P2P5-2 ]
    GeneIDi 57520.
    KEGGi hsa:57520.
    UCSCi uc002utl.1. human. [Q9P2P5-1 ]

    Organism-specific databases

    CTDi 57520.
    GeneCardsi GC02M197064.
    HGNCi HGNC:29853. HECW2.
    HPAi HPA034609.
    HPA044858.
    neXtProti NX_Q9P2P5.
    PharmGKBi PA134925001.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5021.
    HOGENOMi HOG000069940.
    HOVERGENi HBG057414.
    InParanoidi Q9P2P5.
    KOi K12168.
    OMAi TVCSNGP.
    OrthoDBi EOG7BKCTS.
    PhylomeDBi Q9P2P5.
    TreeFami TF313938.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    SignaLinki Q9P2P5.

    Miscellaneous databases

    ChiTaRSi HECW2. human.
    GenomeRNAii 57520.
    NextBioi 63895.
    PROi Q9P2P5.

    Gene expression databases

    ArrayExpressi Q9P2P5.
    Bgeei Q9P2P5.
    CleanExi HS_HECW2.
    Genevestigatori Q9P2P5.

    Family and domain databases

    Gene3Di 2.60.40.150. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR000569. HECT.
    IPR001202. WW_dom.
    [Graphical view ]
    Pfami PF00168. C2. 1 hit.
    PF00632. HECT. 1 hit.
    PF00397. WW. 2 hits.
    [Graphical view ]
    SMARTi SM00239. C2. 1 hit.
    SM00119. HECTc. 1 hit.
    SM00456. WW. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51045. SSF51045. 2 hits.
    SSF56204. SSF56204. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50237. HECT. 1 hit.
    PS01159. WW_DOMAIN_1. 2 hits.
    PS50020. WW_DOMAIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1352-1572 (ISOFORM 1).
      Tissue: Uterus.
    3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Heart and Lung.
    5. "A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and enhances its transcriptional activity."
      Miyazaki K., Ozaki T., Kato C., Hanamoto T., Fujita T., Irino S., Watanabe K., Nakagawa T., Nakagawara A.
      Biochem. Biophys. Res. Commun. 308:106-113(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TP73, TISSUE SPECIFICITY.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHECW2_HUMAN
    AccessioniPrimary (citable) accession number: Q9P2P5
    Secondary accession number(s): B8ZZB4
    , Q17RT5, Q68DF8, Q9NPS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3