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Protein

Rho GTPase-activating protein 28

Gene

ARHGAP28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 28
Alternative name(s):
Rho-type GTPase-activating protein 28
Gene namesi
Name:ARHGAP28
Synonyms:KIAA1314
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:25509. ARHGAP28.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi79822.
OpenTargetsiENSG00000088756.
PharmGKBiPA134915320.

Polymorphism and mutation databases

DMDMi311033539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002804761 – 729Rho GTPase-activating protein 28Add BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineBy similarity1
Modified residuei159PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P2N2.
MaxQBiQ9P2N2.
PaxDbiQ9P2N2.
PeptideAtlasiQ9P2N2.
PRIDEiQ9P2N2.

PTM databases

iPTMnetiQ9P2N2.
PhosphoSitePlusiQ9P2N2.

Expressioni

Tissue specificityi

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000088756.
CleanExiHS_ARHGAP28.
ExpressionAtlasiQ9P2N2. baseline and differential.
GenevisibleiQ9P2N2. HS.

Organism-specific databases

HPAiHPA030413.
HPA030414.
HPA030415.

Interactioni

Protein-protein interaction databases

IntActiQ9P2N2. 3 interactors.
STRINGi9606.ENSP00000313506.

Structurei

3D structure databases

ProteinModelPortaliQ9P2N2.
SMRiQ9P2N2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini380 – 577Rho-GAPPROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2200. Eukaryota.
ENOG410XQ10. LUCA.
GeneTreeiENSGT00760000119123.
HOGENOMiHOG000015106.
HOVERGENiHBG072023.
InParanoidiQ9P2N2.
OMAiDSASMED.
OrthoDBiEOG091G03X0.
PhylomeDBiQ9P2N2.
TreeFamiTF314044.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2N2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI
60 70 80 90 100
NRMLSNESLH PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP
110 120 130 140 150
PAEVTPVDEG ELEAEWLQDV GLSTLISGDE EEDGKALLST LTRTQAAAVQ
160 170 180 190 200
KRYHTYTQTM RKKDKQSIRD VRDIFGVSES PPRDTCGNHT NQLDGTKEER
210 220 230 240 250
ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD SVAILETIPV
260 270 280 290 300
LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL
310 320 330 340 350
KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE
360 370 380 390 400
LTAFFDAFGI QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL
410 420 430 440 450
VLQKFFEKVE ESGLESEGIF RLSGCTAKVK QYREELDAKF NADKFKWDKM
460 470 480 490 500
CHREAAVMLK AFFRELPTSL FPVEYIPAFI SLMERGPHVK VQFQALHLMV
510 520 530 540 550
MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA PNLFFSRSKH
560 570 580 590 600
SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ
610 620 630 640 650
LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL
660 670 680 690 700
SKVSMAIQLN NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE
710 720
HCLDPDAYIL DVYRINPQAE WVIKPQQSS
Length:729
Mass (Da):82,060
Last modified:November 2, 2010 - v3
Checksum:i3272A9A651BB0CF6
GO
Isoform 2 (identifier: Q9P2N2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Show »
Length:670
Mass (Da):75,770
Checksum:i990F02D6A1A582C5
GO
Isoform 3 (identifier: Q9P2N2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-181: SESP → MNEL
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Note: No experimental confirmation available.
Show »
Length:545
Mass (Da):61,956
Checksum:i3C8521D80DB18B44
GO
Isoform 5 (identifier: Q9P2N2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):64,615
Checksum:i47963DC9D216EAA5
GO

Sequence cautioni

The sequence BAA91533 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA92552 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti598K → E in BX648684 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055833168I → V.Corresponds to variant rs2303978dbSNPEnsembl.1
Natural variantiVAR_031155190T → S.Corresponds to variant rs6506448dbSNPEnsembl.1
Natural variantiVAR_031156727Q → P.3 PublicationsCorresponds to variant rs1056408dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0237251 – 177Missing in isoform 3. 2 PublicationsAdd BLAST177
Alternative sequenceiVSP_0399711 – 159Missing in isoform 5. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_0237261 – 52Missing in isoform 2. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_023727178 – 181SESP → MNEL in isoform 3. 2 Publications4
Alternative sequenceiVSP_023728677 – 729SHGSS…PQQSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2 and isoform 3. 3 PublicationsAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037735 mRNA. Translation: BAA92552.1. Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2.
BC065274 mRNA. Translation: AAH65274.1.
AK001174 mRNA. Translation: BAA91533.1. Different initiation.
CCDSiCCDS32785.1. [Q9P2N2-5]
PIRiE59436.
UniGeneiHs.183114.

Genome annotation databases

EnsembliENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756. [Q9P2N2-1]
ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
UCSCiuc002knc.5. human. [Q9P2N2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037735 mRNA. Translation: BAA92552.1. Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2.
BC065274 mRNA. Translation: AAH65274.1.
AK001174 mRNA. Translation: BAA91533.1. Different initiation.
CCDSiCCDS32785.1. [Q9P2N2-5]
PIRiE59436.
UniGeneiHs.183114.

3D structure databases

ProteinModelPortaliQ9P2N2.
SMRiQ9P2N2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9P2N2. 3 interactors.
STRINGi9606.ENSP00000313506.

PTM databases

iPTMnetiQ9P2N2.
PhosphoSitePlusiQ9P2N2.

Polymorphism and mutation databases

DMDMi311033539.

Proteomic databases

EPDiQ9P2N2.
MaxQBiQ9P2N2.
PaxDbiQ9P2N2.
PeptideAtlasiQ9P2N2.
PRIDEiQ9P2N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756. [Q9P2N2-1]
ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
UCSCiuc002knc.5. human. [Q9P2N2-1]

Organism-specific databases

DisGeNETi79822.
GeneCardsiARHGAP28.
H-InvDBHIX0014315.
HGNCiHGNC:25509. ARHGAP28.
HPAiHPA030413.
HPA030414.
HPA030415.
MIMi610592. gene.
neXtProtiNX_Q9P2N2.
OpenTargetsiENSG00000088756.
PharmGKBiPA134915320.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2200. Eukaryota.
ENOG410XQ10. LUCA.
GeneTreeiENSGT00760000119123.
HOGENOMiHOG000015106.
HOVERGENiHBG072023.
InParanoidiQ9P2N2.
OMAiDSASMED.
OrthoDBiEOG091G03X0.
PhylomeDBiQ9P2N2.
TreeFamiTF314044.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ9P2N2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000088756.
CleanExiHS_ARHGAP28.
ExpressionAtlasiQ9P2N2. baseline and differential.
GenevisibleiQ9P2N2. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG28_HUMAN
AccessioniPrimary (citable) accession number: Q9P2N2
Secondary accession number(s): A8MQB7
, A8MU88, Q6P160, Q8N4T3, Q9NW53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 2, 2010
Last modified: November 2, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.