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Q9P2N2 (RHG28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 28
Alternative name(s):
Rho-type GTPase-activating protein 28
Gene names
Name:ARHGAP28
Synonyms:KIAA1314
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state By similarity.

Tissue specificity

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle. Ref.1

Sequence similarities

Contains 1 Rho-GAP domain.

Sequence caution

The sequence BAA91533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAA92552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P2N2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P2N2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST
Isoform 3 (identifier: Q9P2N2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-181: SESP → MNEL
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST
Note: No experimental confirmation available.
Isoform 5 (identifier: Q9P2N2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Rho GTPase-activating protein 28
PRO_0000280476

Regions

Domain380 – 577198Rho-GAP

Amino acid modifications

Modified residue1591Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 177177Missing in isoform 3.
VSP_023725
Alternative sequence1 – 159159Missing in isoform 5.
VSP_039971
Alternative sequence1 – 5252Missing in isoform 2.
VSP_023726
Alternative sequence178 – 1814SESP → MNEL in isoform 3.
VSP_023727
Alternative sequence677 – 72953SHGSS…PQQSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2 and isoform 3.
VSP_023728
Natural variant1681I → V.
Corresponds to variant rs2303978 [ dbSNP | Ensembl ].
VAR_055833
Natural variant1901T → S.
Corresponds to variant rs6506448 [ dbSNP | Ensembl ].
VAR_031155
Natural variant7271Q → P. Ref.1 Ref.4 Ref.5
Corresponds to variant rs1056408 [ dbSNP | Ensembl ].
VAR_031156

Experimental info

Sequence conflict5981K → E in BX648684. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 2, 2010. Version 3.
Checksum: 3272A9A651BB0CF6

FASTA72982,060
        10         20         30         40         50         60 
MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI NRMLSNESLH 

        70         80         90        100        110        120 
PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP PAEVTPVDEG ELEAEWLQDV 

       130        140        150        160        170        180 
GLSTLISGDE EEDGKALLST LTRTQAAAVQ KRYHTYTQTM RKKDKQSIRD VRDIFGVSES 

       190        200        210        220        230        240 
PPRDTCGNHT NQLDGTKEER ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD 

       250        260        270        280        290        300 
SVAILETIPV LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL 

       310        320        330        340        350        360 
KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE LTAFFDAFGI 

       370        380        390        400        410        420 
QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL VLQKFFEKVE ESGLESEGIF 

       430        440        450        460        470        480 
RLSGCTAKVK QYREELDAKF NADKFKWDKM CHREAAVMLK AFFRELPTSL FPVEYIPAFI 

       490        500        510        520        530        540 
SLMERGPHVK VQFQALHLMV MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA 

       550        560        570        580        590        600 
PNLFFSRSKH SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ 

       610        620        630        640        650        660 
LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL SKVSMAIQLN 

       670        680        690        700        710        720 
NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE HCLDPDAYIL DVYRINPQAE 


WVIKPQQSS 

« Hide

Isoform 2 [UniParc].

Checksum: 990F02D6A1A582C5
Show »

FASTA67075,770
Isoform 3 [UniParc].

Checksum: 3C8521D80DB18B44
Show »

FASTA54561,956
Isoform 5 [UniParc].

Checksum: 47963DC9D216EAA5
Show »

FASTA57064,615

References

[1]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANT PRO-727.
Tissue: Brain.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[3]"DNA sequence and analysis of human chromosome 18."
Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. expand/collapse author list , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S.
Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-729 (ISOFORM 1), VARIANT PRO-727.
Tissue: Brain and Skin.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 298-729 (ISOFORM 3), VARIANT PRO-727.
[6]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB037735 mRNA. Translation: BAA92552.1. Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2.
BC065274 mRNA. Translation: AAH65274.1.
AK001174 mRNA. Translation: BAA91533.1. Different initiation.
CCDSCCDS32785.1. [Q9P2N2-5]
PIRE59436.
UniGeneHs.183114.

3D structure databases

ProteinModelPortalQ9P2N2.
SMRQ9P2N2. Positions 377-587.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9P2N2.

Polymorphism databases

DMDM311033539.

Proteomic databases

MaxQBQ9P2N2.
PaxDbQ9P2N2.
PRIDEQ9P2N2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756. [Q9P2N2-1]
ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
KEGGhsa:79822.
UCSCuc002knc.3. human. [Q9P2N2-2]
uc002kne.3. human. [Q9P2N2-1]
uc002knf.3. human. [Q9P2N2-3]

Organism-specific databases

GeneCardsGC18P006730.
H-InvDBHIX0014315.
HGNCHGNC:25509. ARHGAP28.
HPAHPA030413.
HPA030414.
HPA030415.
MIM610592. gene.
neXtProtNX_Q9P2N2.
PharmGKBPA134915320.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG329973.
HOGENOMHOG000015106.
HOVERGENHBG072023.
InParanoidQ9P2N2.
OMADSASMED.
OrthoDBEOG7ZGX3J.
PhylomeDBQ9P2N2.
TreeFamTF314044.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ9P2N2.
BgeeQ9P2N2.
CleanExHS_ARHGAP28.
GenevestigatorQ9P2N2.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
InterProIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
PROSITEPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi79822.
NextBio13653754.
PROQ9P2N2.
SOURCESearch...

Entry information

Entry nameRHG28_HUMAN
AccessionPrimary (citable) accession number: Q9P2N2
Secondary accession number(s): A8MQB7 expand/collapse secondary AC list , A8MU88, Q6P160, Q8N4T3, Q9NW53
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 2, 2010
Last modified: July 9, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM