Reviewed, UniProtKB/Swiss-Prot Q9P2N2 (RHG28_HUMAN)

Names and origin

Protein names

Recommended name:
    Rho GTPase-activating protein 28
Alternative name(s):
    Rho-type GTPase-activating protein 28

Gene names

Name:	ARHGAP28
Synonyms:	KIAA1314

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state By similarity.
Tissue specificity	Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Contains 1 Rho-GAP domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P2N2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P2N2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     677-729: SHGSSECIKI...EWVIKPQPSS → RILHWQRAAL...LFTIGLDIST

Isoform 3 (identifier: Q9P2N2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-181: SESP → MNEL
     677-729: SHGSSECIKI...EWVIKPQPSS → RILHWQRAAL...LFTIGLDIST

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9P2N2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     677-729: SHGSSECIKI...EWVIKPQPSS → RILHWQRAAL...LFTIGLDIST

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	Rho GTPase-activating protein 28		PRO_0000280476
Regions
	Domain	380 – 577	198	Rho-GAP
Amino acid modifications
	Modified residue	155	1	Phosphothreonine By similarity
	Modified residue	157	1	Phosphothreonine By similarity
	Modified residue	159	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 177	177	Missing in isoform 3.		VSP_023725
	Alternative sequence	1 – 52	52	Missing in isoform 2.		VSP_023726
	Alternative sequence	178 – 181	4	SESP → MNEL in isoform 3.		VSP_023727
	Alternative sequence	677 – 729	53	SHGSS…PQPSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2, isoform 3 and isoform 4.		VSP_023728
	Natural variant	168	1	I → V: dbSNP rs2303978.		VAR_055833
	Natural variant	190	1	T → S: dbSNP rs6506448.		VAR_031155
	Natural variant	727	1	P → Q: dbSNP rs1056408. Ref.2		VAR_031156

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 20, 2007. Version 2. Checksum: 3272A81651BB0CF6	FASTA	729	82,029
	10 20 30 40 50 60 MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI NRMLSNESLH 70 80 90 100 110 120 PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP PAEVTPVDEG ELEAEWLQDV 130 140 150 160 170 180 GLSTLISGDE EEDGKALLST LTRTQAAAVQ KRYHTYTQTM RKKDKQSIRD VRDIFGVSES 190 200 210 220 230 240 PPRDTCGNHT NQLDGTKEER ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD 250 260 270 280 290 300 SVAILETIPV LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL 310 320 330 340 350 360 KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE LTAFFDAFGI 370 380 390 400 410 420 QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL VLQKFFEKVE ESGLESEGIF 430 440 450 460 470 480 RLSGCTAKVK QYREELDAKF NADKFKWDKM CHREAAVMLK AFFRELPTSL FPVEYIPAFI 490 500 510 520 530 540 SLMERGPHVK VQFQALHLMV MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA 550 560 570 580 590 600 PNLFFSRSKH SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ 610 620 630 640 650 660 LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL SKVSMAIQLN 670 680 690 700 710 720 NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE HCLDPDAYIL DVYRINPQAE WVIKPQPSS
	Isoform 2. Checksum: 990F02D6A1A582C5	FASTA	670	75,770
	10 20 30 40 50 60 MLSNESLHPP AFSRSNSEAS VDSASMEDFW REIESIKDSS MGGQEEPPPA EVTPVDEGEL 70 80 90 100 110 120 EAEWLQDVGL STLISGDEEE DGKALLSTLT RTQAAAVQKR YHTYTQTMRK KDKQSIRDVR 130 140 150 160 170 180 DIFGVSESPP RDTCGNHTNQ LDGTKEEREL PRVIKTSGSM PDDASLNSTT LSDASQDKEG 190 200 210 220 230 240 SFAVPRSDSV AILETIPVLP VHSNGSPEPG QPVQNAISDD DFLEKNIPPE AEELSFEVSY 250 260 270 280 290 300 SEMVTEALKR NKLKKSEIKK EDYVLTKFNV QKTRFGLTEA GDLSAEDMKK IRHLSLIELT 310 320 330 340 350 360 AFFDAFGIQL KRNKTEKVKG RDNGIFGVPL TVLLDGDRKK DPGVKVPLVL QKFFEKVEES 370 380 390 400 410 420 GLESEGIFRL SGCTAKVKQY REELDAKFNA DKFKWDKMCH REAAVMLKAF FRELPTSLFP 430 440 450 460 470 480 VEYIPAFISL MERGPHVKVQ FQALHLMVMA LPDANRDAAQ ALMTFFNKVI ANESKNRMSL 490 500 510 520 530 540 WNISTVMAPN LFFSRSKHSD YEELLLANTA AHIIRLMLKY QKILWKVPSF LITQVRRMNE 550 560 570 580 590 600 ATMLLKKQLP SVRKLLRRKT LERETASPKT SKVLQKSPSA RRMSDVPEGV IRVHAPLLSK 610 620 630 640 650 660 VSMAIQLNNQ TKAKDILAKF QYENRILHWQ RAALSFLNGK WVKKEREEST ETNRSPKHVF 670 LFTIGLDIST
	Isoform 3. Checksum: 3C8521D80DB18B44	FASTA	545	61,956
	10 20 30 40 50 60 MNELPRDTCG NHTNQLDGTK EERELPRVIK TSGSMPDDAS LNSTTLSDAS QDKEGSFAVP 70 80 90 100 110 120 RSDSVAILET IPVLPVHSNG SPEPGQPVQN AISDDDFLEK NIPPEAEELS FEVSYSEMVT 130 140 150 160 170 180 EALKRNKLKK SEIKKEDYVL TKFNVQKTRF GLTEAGDLSA EDMKKIRHLS LIELTAFFDA 190 200 210 220 230 240 FGIQLKRNKT EKVKGRDNGI FGVPLTVLLD GDRKKDPGVK VPLVLQKFFE KVEESGLESE 250 260 270 280 290 300 GIFRLSGCTA KVKQYREELD AKFNADKFKW DKMCHREAAV MLKAFFRELP TSLFPVEYIP 310 320 330 340 350 360 AFISLMERGP HVKVQFQALH LMVMALPDAN RDAAQALMTF FNKVIANESK NRMSLWNIST 370 380 390 400 410 420 VMAPNLFFSR SKHSDYEELL LANTAAHIIR LMLKYQKILW KVPSFLITQV RRMNEATMLL 430 440 450 460 470 480 KKQLPSVRKL LRRKTLERET ASPKTSKVLQ KSPSARRMSD VPEGVIRVHA PLLSKVSMAI 490 500 510 520 530 540 QLNNQTKAKD ILAKFQYENR ILHWQRAALS FLNGKWVKKE REESTETNRS PKHVFLFTIG LDIST
	Isoform 4. Checksum: 1612A420EC6D09DF	FASTA	722	81,486
	10 20 30 40 50 60 MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI NRMLSNESLH 70 80 90 100 110 120 PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP PAEVTPVDEG ELEAEWLQDV 130 140 150 160 170 180 GLSTLISGDE EEDGKALLST LTRTQAAAVQ KRYHTYTQTM RKKDKQSIRD VRDIFGVSES 190 200 210 220 230 240 PPRDTCGNHT NQLDGTKEER ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD 250 260 270 280 290 300 SVAILETIPV LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL 310 320 330 340 350 360 KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE LTAFFDAFGI 370 380 390 400 410 420 QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL VLQKFFEKVE ESGLESEGIF 430 440 450 460 470 480 RLSGCTAKVK QYREELDAKF NADKFKWDKM CHREAAVMLK AFFRELPTSL FPVEYIPAFI 490 500 510 520 530 540 SLMERGPHVK VQFQALHLMV MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA 550 560 570 580 590 600 PNLFFSRSKH SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ 610 620 630 640 650 660 LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL SKVSMAIQLN 670 680 690 700 710 720 NQTKAKDILA KFQYENRILH WQRAALSFLN GKWVKKEREE STETNRSPKH VFLFTIGLDI ST

References

[1]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Brain.
[2]	"DNA sequence and analysis of human chromosome 18." Nusbaum C., Zody M.C., Borowsky M.L., Kamal M., Kodira C.D., Taylor T.D., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Abouelleil A., Allen N.R., Anderson S., Bloom T., Bugalter B., Butler J. , Cook A., DeCaprio D., Engels R., Garber M., Gnirke A., Hafez N., Hall J.L., Norman C.H., Itoh T., Jaffe D.B., Kuroki Y., Lehoczky J., Lui A., Macdonald P., Mauceli E., Mikkelsen T.S., Naylor J.W., Nicol R., Nguyen C., Noguchi H., O'Leary S.B., Piqani B., Smith C.L., Talamas J.A., Topham K., Totoki Y., Toyoda A., Wain H.M., Young S.K., Zeng Q., Zimmer A.R., Fujiyama A., Hattori M., Birren B.W., Sakaki Y., Lander E.S. Nature 437:551-555(2005) [PubMed: 16177791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLN-727.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-729 (ISOFORM 1). Tissue: Brain and Skin.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 298-729.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB037735 mRNA. Translation: BAA92552.1. Different initiation. AP005205 Genomic DNA. No translation available. AP005210 Genomic DNA. No translation available. BC033668 mRNA. Translation: AAH33668.2. BC065274 mRNA. Translation: AAH65274.1. AK001174 mRNA. Translation: BAA91533.1. Different initiation.
IPI	IPI00002392. IPI00296793. IPI00743001. IPI00830014.
PIR	E59436.
RefSeq	NP_001010000.1. NP_109597.2.
UniGene	Hs.183114
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9P2N2.
PTM databases
PhosphoSite	Q9P2N2.
Genome annotation databases
Ensembl	ENST00000419673; ENSP00000392660; ENSG00000088756; Homo sapiens. [Genome view]
GeneID	79822.
KEGG	hsa:79822.
UCSC	uc002knf.1. human.
Organism-specific databases
CTD	79822.
GeneCards	GC18P006781.
HGNC	HGNC:25509. ARHGAP28.
MIM	610592. gene.
PharmGKB	PA134915320.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9P2N2.
OMA	SSRRMSD
OrthoDB	EOG9FXTSW
Enzyme and pathway databases
Reactome	REACT_11044. Signaling by Rho GTPases.
Gene expression databases
ArrayExpress	Q9P2N2.
Bgee	Q9P2N2.
CleanEx	HS_ARHGAP28.
Genevestigator	Q9P2N2.
Family and domain databases
InterPro	IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	69444.
SOURCE	Search...

Entry information

Entry name

RHG28_HUMAN

Accession

Primary (citable) accession number: Q9P2N2
Secondary accession number(s): Q6P160, Q8N4T3, Q9NW53

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	March 20, 2007
Last modified:	November 24, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.