Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9P2N2

- RHG28_HUMAN

UniProt

Q9P2N2 - RHG28_HUMAN

Protein

Rho GTPase-activating protein 28

Gene

ARHGAP28

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 3 (02 Nov 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

    GO - Molecular functioni

    1. GTPase activator activity Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of small GTPase mediated signal transduction Source: Reactome
    2. small GTPase mediated signal transduction Source: Reactome

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    ReactomeiREACT_11051. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho GTPase-activating protein 28
    Alternative name(s):
    Rho-type GTPase-activating protein 28
    Gene namesi
    Name:ARHGAP28
    Synonyms:KIAA1314
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:25509. ARHGAP28.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: HPA
    2. cytosol Source: Reactome
    3. nucleus Source: HPA

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134915320.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 729729Rho GTPase-activating protein 28PRO_0000280476Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei159 – 1591PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9P2N2.
    PaxDbiQ9P2N2.
    PRIDEiQ9P2N2.

    PTM databases

    PhosphoSiteiQ9P2N2.

    Expressioni

    Tissue specificityi

    Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ9P2N2.
    BgeeiQ9P2N2.
    CleanExiHS_ARHGAP28.
    GenevestigatoriQ9P2N2.

    Organism-specific databases

    HPAiHPA030413.
    HPA030414.
    HPA030415.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P2N2.
    SMRiQ9P2N2. Positions 377-587.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini380 – 577198Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG329973.
    HOGENOMiHOG000015106.
    HOVERGENiHBG072023.
    InParanoidiQ9P2N2.
    OMAiDSASMED.
    OrthoDBiEOG7ZGX3J.
    PhylomeDBiQ9P2N2.
    TreeFamiTF314044.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    InterProiIPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    [Graphical view]
    PfamiPF00620. RhoGAP. 1 hit.
    [Graphical view]
    SMARTiSM00324. RhoGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    PROSITEiPS50238. RHOGAP. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9P2N2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI    50
    NRMLSNESLH PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP 100
    PAEVTPVDEG ELEAEWLQDV GLSTLISGDE EEDGKALLST LTRTQAAAVQ 150
    KRYHTYTQTM RKKDKQSIRD VRDIFGVSES PPRDTCGNHT NQLDGTKEER 200
    ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD SVAILETIPV 250
    LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL 300
    KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE 350
    LTAFFDAFGI QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL 400
    VLQKFFEKVE ESGLESEGIF RLSGCTAKVK QYREELDAKF NADKFKWDKM 450
    CHREAAVMLK AFFRELPTSL FPVEYIPAFI SLMERGPHVK VQFQALHLMV 500
    MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA PNLFFSRSKH 550
    SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ 600
    LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL 650
    SKVSMAIQLN NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE 700
    HCLDPDAYIL DVYRINPQAE WVIKPQQSS 729
    Length:729
    Mass (Da):82,060
    Last modified:November 2, 2010 - v3
    Checksum:i3272A9A651BB0CF6
    GO
    Isoform 2 (identifier: Q9P2N2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-52: Missing.
         677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

    Show »
    Length:670
    Mass (Da):75,770
    Checksum:i990F02D6A1A582C5
    GO
    Isoform 3 (identifier: Q9P2N2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-177: Missing.
         178-181: SESP → MNEL
         677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

    Note: No experimental confirmation available.

    Show »
    Length:545
    Mass (Da):61,956
    Checksum:i3C8521D80DB18B44
    GO
    Isoform 5 (identifier: Q9P2N2-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-159: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:570
    Mass (Da):64,615
    Checksum:i47963DC9D216EAA5
    GO

    Sequence cautioni

    The sequence BAA91533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAA92552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti598 – 5981K → E in BX648684. (PubMed:17974005)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti168 – 1681I → V.
    Corresponds to variant rs2303978 [ dbSNP | Ensembl ].
    VAR_055833
    Natural varianti190 – 1901T → S.
    Corresponds to variant rs6506448 [ dbSNP | Ensembl ].
    VAR_031155
    Natural varianti727 – 7271Q → P.3 Publications
    Corresponds to variant rs1056408 [ dbSNP | Ensembl ].
    VAR_031156

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 177177Missing in isoform 3. 2 PublicationsVSP_023725Add
    BLAST
    Alternative sequencei1 – 159159Missing in isoform 5. 1 PublicationVSP_039971Add
    BLAST
    Alternative sequencei1 – 5252Missing in isoform 2. 1 PublicationVSP_023726Add
    BLAST
    Alternative sequencei178 – 1814SESP → MNEL in isoform 3. 2 PublicationsVSP_023727
    Alternative sequencei677 – 72953SHGSS…PQQSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2 and isoform 3. 3 PublicationsVSP_023728Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB037735 mRNA. Translation: BAA92552.1. Different initiation.
    BX648684 mRNA. No translation available.
    AP005205 Genomic DNA. No translation available.
    AP005210 Genomic DNA. No translation available.
    BC033668 mRNA. Translation: AAH33668.2.
    BC065274 mRNA. Translation: AAH65274.1.
    AK001174 mRNA. Translation: BAA91533.1. Different initiation.
    CCDSiCCDS32785.1. [Q9P2N2-5]
    PIRiE59436.
    UniGeneiHs.183114.

    Genome annotation databases

    EnsembliENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
    ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
    ENST00000383472; ENSP00000372964; ENSG00000088756. [Q9P2N2-1]
    ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
    ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
    UCSCiuc002knc.3. human. [Q9P2N2-2]
    uc002kne.3. human. [Q9P2N2-1]
    uc002knf.3. human. [Q9P2N2-3]

    Polymorphism databases

    DMDMi311033539.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB037735 mRNA. Translation: BAA92552.1 . Different initiation.
    BX648684 mRNA. No translation available.
    AP005205 Genomic DNA. No translation available.
    AP005210 Genomic DNA. No translation available.
    BC033668 mRNA. Translation: AAH33668.2 .
    BC065274 mRNA. Translation: AAH65274.1 .
    AK001174 mRNA. Translation: BAA91533.1 . Different initiation.
    CCDSi CCDS32785.1. [Q9P2N2-5 ]
    PIRi E59436.
    UniGenei Hs.183114.

    3D structure databases

    ProteinModelPortali Q9P2N2.
    SMRi Q9P2N2. Positions 377-587.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9P2N2.

    Polymorphism databases

    DMDMi 311033539.

    Proteomic databases

    MaxQBi Q9P2N2.
    PaxDbi Q9P2N2.
    PRIDEi Q9P2N2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262227 ; ENSP00000262227 ; ENSG00000088756 . [Q9P2N2-2 ]
    ENST00000314319 ; ENSP00000313506 ; ENSG00000088756 . [Q9P2N2-5 ]
    ENST00000383472 ; ENSP00000372964 ; ENSG00000088756 . [Q9P2N2-1 ]
    ENST00000419673 ; ENSP00000392660 ; ENSG00000088756 . [Q9P2N2-5 ]
    ENST00000532996 ; ENSP00000435990 ; ENSG00000088756 . [Q9P2N2-3 ]
    UCSCi uc002knc.3. human. [Q9P2N2-2 ]
    uc002kne.3. human. [Q9P2N2-1 ]
    uc002knf.3. human. [Q9P2N2-3 ]

    Organism-specific databases

    GeneCardsi GC18P006730.
    H-InvDB HIX0014315.
    HGNCi HGNC:25509. ARHGAP28.
    HPAi HPA030413.
    HPA030414.
    HPA030415.
    MIMi 610592. gene.
    neXtProti NX_Q9P2N2.
    PharmGKBi PA134915320.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG329973.
    HOGENOMi HOG000015106.
    HOVERGENi HBG072023.
    InParanoidi Q9P2N2.
    OMAi DSASMED.
    OrthoDBi EOG7ZGX3J.
    PhylomeDBi Q9P2N2.
    TreeFami TF314044.

    Enzyme and pathway databases

    Reactomei REACT_11051. Rho GTPase cycle.

    Miscellaneous databases

    GenomeRNAii 79822.
    NextBioi 13653754.
    PROi Q9P2N2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9P2N2.
    Bgeei Q9P2N2.
    CleanExi HS_ARHGAP28.
    Genevestigatori Q9P2N2.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    InterProi IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    [Graphical view ]
    Pfami PF00620. RhoGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00324. RhoGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    PROSITEi PS50238. RHOGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANT PRO-727.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-729 (ISOFORM 1), VARIANT PRO-727.
      Tissue: Brain and Skin.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 298-729 (ISOFORM 3), VARIANT PRO-727.
    6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRHG28_HUMAN
    AccessioniPrimary (citable) accession number: Q9P2N2
    Secondary accession number(s): A8MQB7
    , A8MU88, Q6P160, Q8N4T3, Q9NW53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 101 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3