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Q9P2N2

- RHG28_HUMAN

UniProt

Q9P2N2 - RHG28_HUMAN

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Protein
Rho GTPase-activating protein 28
Gene
ARHGAP28, KIAA1314
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state By similarity.

GO - Molecular functioni

  1. GTPase activator activity Source: UniProtKB-KW

GO - Biological processi

  1. regulation of small GTPase mediated signal transduction Source: Reactome
  2. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 28
Alternative name(s):
Rho-type GTPase-activating protein 28
Gene namesi
Synonyms:KIAA1314
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:25509. ARHGAP28.

Subcellular locationi

GO - Cellular componenti

  1. cell junction Source: HPA
  2. cytosol Source: Reactome
  3. nucleus Source: HPA
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134915320.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Rho GTPase-activating protein 28
PRO_0000280476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P2N2.
PaxDbiQ9P2N2.
PRIDEiQ9P2N2.

PTM databases

PhosphoSiteiQ9P2N2.

Expressioni

Tissue specificityi

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.1 Publication

Gene expression databases

ArrayExpressiQ9P2N2.
BgeeiQ9P2N2.
CleanExiHS_ARHGAP28.
GenevestigatoriQ9P2N2.

Organism-specific databases

HPAiHPA030413.
HPA030414.
HPA030415.

Structurei

3D structure databases

ProteinModelPortaliQ9P2N2.
SMRiQ9P2N2. Positions 377-587.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini380 – 577198Rho-GAP
Add
BLAST

Sequence similaritiesi

Contains 1 Rho-GAP domain.

Phylogenomic databases

eggNOGiNOG329973.
HOGENOMiHOG000015106.
HOVERGENiHBG072023.
InParanoidiQ9P2N2.
OMAiDSASMED.
OrthoDBiEOG7ZGX3J.
PhylomeDBiQ9P2N2.
TreeFamiTF314044.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9P2N2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI    50
NRMLSNESLH PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP 100
PAEVTPVDEG ELEAEWLQDV GLSTLISGDE EEDGKALLST LTRTQAAAVQ 150
KRYHTYTQTM RKKDKQSIRD VRDIFGVSES PPRDTCGNHT NQLDGTKEER 200
ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD SVAILETIPV 250
LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL 300
KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE 350
LTAFFDAFGI QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL 400
VLQKFFEKVE ESGLESEGIF RLSGCTAKVK QYREELDAKF NADKFKWDKM 450
CHREAAVMLK AFFRELPTSL FPVEYIPAFI SLMERGPHVK VQFQALHLMV 500
MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA PNLFFSRSKH 550
SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ 600
LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL 650
SKVSMAIQLN NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE 700
HCLDPDAYIL DVYRINPQAE WVIKPQQSS 729
Length:729
Mass (Da):82,060
Last modified:November 2, 2010 - v3
Checksum:i3272A9A651BB0CF6
GO
Isoform 2 (identifier: Q9P2N2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Show »
Length:670
Mass (Da):75,770
Checksum:i990F02D6A1A582C5
GO
Isoform 3 (identifier: Q9P2N2-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-181: SESP → MNEL
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Note: No experimental confirmation available.

Show »
Length:545
Mass (Da):61,956
Checksum:i3C8521D80DB18B44
GO
Isoform 5 (identifier: Q9P2N2-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.

Note: No experimental confirmation available.

Show »
Length:570
Mass (Da):64,615
Checksum:i47963DC9D216EAA5
GO

Sequence cautioni

The sequence BAA91533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAA92552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti168 – 1681I → V.
Corresponds to variant rs2303978 [ dbSNP | Ensembl ].
VAR_055833
Natural varianti190 – 1901T → S.
Corresponds to variant rs6506448 [ dbSNP | Ensembl ].
VAR_031155
Natural varianti727 – 7271Q → P.3 Publications
Corresponds to variant rs1056408 [ dbSNP | Ensembl ].
VAR_031156

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 177177Missing in isoform 3.
VSP_023725Add
BLAST
Alternative sequencei1 – 159159Missing in isoform 5.
VSP_039971Add
BLAST
Alternative sequencei1 – 5252Missing in isoform 2.
VSP_023726Add
BLAST
Alternative sequencei178 – 1814SESP → MNEL in isoform 3.
VSP_023727
Alternative sequencei677 – 72953SHGSS…PQQSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2 and isoform 3.
VSP_023728Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti598 – 5981K → E in BX648684. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB037735 mRNA. Translation: BAA92552.1. Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2.
BC065274 mRNA. Translation: AAH65274.1.
AK001174 mRNA. Translation: BAA91533.1. Different initiation.
CCDSiCCDS32785.1. [Q9P2N2-5]
PIRiE59436.
UniGeneiHs.183114.

Genome annotation databases

EnsembliENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756. [Q9P2N2-1]
ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
UCSCiuc002knc.3. human. [Q9P2N2-2]
uc002kne.3. human. [Q9P2N2-1]
uc002knf.3. human. [Q9P2N2-3]

Polymorphism databases

DMDMi311033539.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB037735 mRNA. Translation: BAA92552.1 . Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2 .
BC065274 mRNA. Translation: AAH65274.1 .
AK001174 mRNA. Translation: BAA91533.1 . Different initiation.
CCDSi CCDS32785.1. [Q9P2N2-5 ]
PIRi E59436.
UniGenei Hs.183114.

3D structure databases

ProteinModelPortali Q9P2N2.
SMRi Q9P2N2. Positions 377-587.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9P2N2.

Polymorphism databases

DMDMi 311033539.

Proteomic databases

MaxQBi Q9P2N2.
PaxDbi Q9P2N2.
PRIDEi Q9P2N2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000262227 ; ENSP00000262227 ; ENSG00000088756 . [Q9P2N2-2 ]
ENST00000314319 ; ENSP00000313506 ; ENSG00000088756 . [Q9P2N2-5 ]
ENST00000383472 ; ENSP00000372964 ; ENSG00000088756 . [Q9P2N2-1 ]
ENST00000419673 ; ENSP00000392660 ; ENSG00000088756 . [Q9P2N2-5 ]
ENST00000532996 ; ENSP00000435990 ; ENSG00000088756 . [Q9P2N2-3 ]
UCSCi uc002knc.3. human. [Q9P2N2-2 ]
uc002kne.3. human. [Q9P2N2-1 ]
uc002knf.3. human. [Q9P2N2-3 ]

Organism-specific databases

GeneCardsi GC18P006730.
H-InvDB HIX0014315.
HGNCi HGNC:25509. ARHGAP28.
HPAi HPA030413.
HPA030414.
HPA030415.
MIMi 610592. gene.
neXtProti NX_Q9P2N2.
PharmGKBi PA134915320.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG329973.
HOGENOMi HOG000015106.
HOVERGENi HBG072023.
InParanoidi Q9P2N2.
OMAi DSASMED.
OrthoDBi EOG7ZGX3J.
PhylomeDBi Q9P2N2.
TreeFami TF314044.

Enzyme and pathway databases

Reactomei REACT_11051. Rho GTPase cycle.

Miscellaneous databases

GenomeRNAii 79822.
NextBioi 13653754.
PROi Q9P2N2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9P2N2.
Bgeei Q9P2N2.
CleanExi HS_ARHGAP28.
Genevestigatori Q9P2N2.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
InterProi IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view ]
Pfami PF00620. RhoGAP. 1 hit.
[Graphical view ]
SMARTi SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS50238. RHOGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANT PRO-727.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-729 (ISOFORM 1), VARIANT PRO-727.
    Tissue: Brain and Skin.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 298-729 (ISOFORM 3), VARIANT PRO-727.
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHG28_HUMAN
AccessioniPrimary (citable) accession number: Q9P2N2
Secondary accession number(s): A8MQB7
, A8MU88, Q6P160, Q8N4T3, Q9NW53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 2, 2010
Last modified: September 3, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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