Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rho GTPase-activating protein 28

Gene

ARHGAP28

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 28
Alternative name(s):
Rho-type GTPase-activating protein 28
Gene namesi
Name:ARHGAP28
Synonyms:KIAA1314
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:25509. ARHGAP28.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134915320.

Polymorphism and mutation databases

DMDMi311033539.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Rho GTPase-activating protein 28PRO_0000280476Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P2N2.
PaxDbiQ9P2N2.
PRIDEiQ9P2N2.

PTM databases

PhosphoSiteiQ9P2N2.

Expressioni

Tissue specificityi

Expressed in testis. Expressed at moderate level in kidney and ovary, and weakly expressed in spleen and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ9P2N2.
CleanExiHS_ARHGAP28.
ExpressionAtlasiQ9P2N2. baseline and differential.

Organism-specific databases

HPAiHPA030413.
HPA030414.
HPA030415.

Interactioni

Protein-protein interaction databases

IntActiQ9P2N2. 1 interaction.
STRINGi9606.ENSP00000313506.

Structurei

3D structure databases

ProteinModelPortaliQ9P2N2.
SMRiQ9P2N2. Positions 377-587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini380 – 577198Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG329973.
GeneTreeiENSGT00760000119123.
HOGENOMiHOG000015106.
HOVERGENiHBG072023.
InParanoidiQ9P2N2.
OMAiDSASMED.
OrthoDBiEOG7ZGX3J.
PhylomeDBiQ9P2N2.
TreeFamiTF314044.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2N2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVEDSGGVV LTAYHSYARA QPPNAESRCA PRAAASHPLS RKSIPRCRRI
60 70 80 90 100
NRMLSNESLH PPAFSRSNSE ASVDSASMED FWREIESIKD SSMGGQEEPP
110 120 130 140 150
PAEVTPVDEG ELEAEWLQDV GLSTLISGDE EEDGKALLST LTRTQAAAVQ
160 170 180 190 200
KRYHTYTQTM RKKDKQSIRD VRDIFGVSES PPRDTCGNHT NQLDGTKEER
210 220 230 240 250
ELPRVIKTSG SMPDDASLNS TTLSDASQDK EGSFAVPRSD SVAILETIPV
260 270 280 290 300
LPVHSNGSPE PGQPVQNAIS DDDFLEKNIP PEAEELSFEV SYSEMVTEAL
310 320 330 340 350
KRNKLKKSEI KKEDYVLTKF NVQKTRFGLT EAGDLSAEDM KKIRHLSLIE
360 370 380 390 400
LTAFFDAFGI QLKRNKTEKV KGRDNGIFGV PLTVLLDGDR KKDPGVKVPL
410 420 430 440 450
VLQKFFEKVE ESGLESEGIF RLSGCTAKVK QYREELDAKF NADKFKWDKM
460 470 480 490 500
CHREAAVMLK AFFRELPTSL FPVEYIPAFI SLMERGPHVK VQFQALHLMV
510 520 530 540 550
MALPDANRDA AQALMTFFNK VIANESKNRM SLWNISTVMA PNLFFSRSKH
560 570 580 590 600
SDYEELLLAN TAAHIIRLML KYQKILWKVP SFLITQVRRM NEATMLLKKQ
610 620 630 640 650
LPSVRKLLRR KTLERETASP KTSKVLQKSP SARRMSDVPE GVIRVHAPLL
660 670 680 690 700
SKVSMAIQLN NQTKAKDILA KFQYENSHGS SECIKIQNQR LYEIGGNIGE
710 720
HCLDPDAYIL DVYRINPQAE WVIKPQQSS
Length:729
Mass (Da):82,060
Last modified:November 2, 2010 - v3
Checksum:i3272A9A651BB0CF6
GO
Isoform 2 (identifier: Q9P2N2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Show »
Length:670
Mass (Da):75,770
Checksum:i990F02D6A1A582C5
GO
Isoform 3 (identifier: Q9P2N2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-177: Missing.
     178-181: SESP → MNEL
     677-729: SHGSSECIKI...EWVIKPQQSS → RILHWQRAAL...LFTIGLDIST

Note: No experimental confirmation available.
Show »
Length:545
Mass (Da):61,956
Checksum:i3C8521D80DB18B44
GO
Isoform 5 (identifier: Q9P2N2-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):64,615
Checksum:i47963DC9D216EAA5
GO

Sequence cautioni

The sequence BAA91533.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA92552.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti598 – 5981K → E in BX648684 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti168 – 1681I → V.
Corresponds to variant rs2303978 [ dbSNP | Ensembl ].
VAR_055833
Natural varianti190 – 1901T → S.
Corresponds to variant rs6506448 [ dbSNP | Ensembl ].
VAR_031155
Natural varianti727 – 7271Q → P.3 Publications
Corresponds to variant rs1056408 [ dbSNP | Ensembl ].
VAR_031156

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 177177Missing in isoform 3. 2 PublicationsVSP_023725Add
BLAST
Alternative sequencei1 – 159159Missing in isoform 5. 1 PublicationVSP_039971Add
BLAST
Alternative sequencei1 – 5252Missing in isoform 2. 1 PublicationVSP_023726Add
BLAST
Alternative sequencei178 – 1814SESP → MNEL in isoform 3. 2 PublicationsVSP_023727
Alternative sequencei677 – 72953SHGSS…PQQSS → RILHWQRAALSFLNGKWVKK EREESTETNRSPKHVFLFTI GLDIST in isoform 2 and isoform 3. 3 PublicationsVSP_023728Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037735 mRNA. Translation: BAA92552.1. Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2.
BC065274 mRNA. Translation: AAH65274.1.
AK001174 mRNA. Translation: BAA91533.1. Different initiation.
CCDSiCCDS32785.1. [Q9P2N2-5]
PIRiE59436.
UniGeneiHs.183114.

Genome annotation databases

EnsembliENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756.
ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
UCSCiuc002knc.3. human. [Q9P2N2-2]
uc002kne.3. human. [Q9P2N2-1]
uc002knf.3. human. [Q9P2N2-3]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037735 mRNA. Translation: BAA92552.1. Different initiation.
BX648684 mRNA. No translation available.
AP005205 Genomic DNA. No translation available.
AP005210 Genomic DNA. No translation available.
BC033668 mRNA. Translation: AAH33668.2.
BC065274 mRNA. Translation: AAH65274.1.
AK001174 mRNA. Translation: BAA91533.1. Different initiation.
CCDSiCCDS32785.1. [Q9P2N2-5]
PIRiE59436.
UniGeneiHs.183114.

3D structure databases

ProteinModelPortaliQ9P2N2.
SMRiQ9P2N2. Positions 377-587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9P2N2. 1 interaction.
STRINGi9606.ENSP00000313506.

PTM databases

PhosphoSiteiQ9P2N2.

Polymorphism and mutation databases

DMDMi311033539.

Proteomic databases

MaxQBiQ9P2N2.
PaxDbiQ9P2N2.
PRIDEiQ9P2N2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262227; ENSP00000262227; ENSG00000088756. [Q9P2N2-2]
ENST00000314319; ENSP00000313506; ENSG00000088756. [Q9P2N2-5]
ENST00000383472; ENSP00000372964; ENSG00000088756.
ENST00000419673; ENSP00000392660; ENSG00000088756. [Q9P2N2-5]
ENST00000532996; ENSP00000435990; ENSG00000088756. [Q9P2N2-3]
UCSCiuc002knc.3. human. [Q9P2N2-2]
uc002kne.3. human. [Q9P2N2-1]
uc002knf.3. human. [Q9P2N2-3]

Organism-specific databases

GeneCardsiGC18P006730.
H-InvDBHIX0014315.
HGNCiHGNC:25509. ARHGAP28.
HPAiHPA030413.
HPA030414.
HPA030415.
MIMi610592. gene.
neXtProtiNX_Q9P2N2.
PharmGKBiPA134915320.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG329973.
GeneTreeiENSGT00760000119123.
HOGENOMiHOG000015106.
HOVERGENiHBG072023.
InParanoidiQ9P2N2.
OMAiDSASMED.
OrthoDBiEOG7ZGX3J.
PhylomeDBiQ9P2N2.
TreeFamiTF314044.

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.

Miscellaneous databases

NextBioi13653754.
PROiQ9P2N2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9P2N2.
CleanExiHS_ARHGAP28.
ExpressionAtlasiQ9P2N2. baseline and differential.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANT PRO-727.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 334-729 (ISOFORM 1), VARIANT PRO-727.
    Tissue: Brain and Skin.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 298-729 (ISOFORM 3), VARIANT PRO-727.
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRHG28_HUMAN
AccessioniPrimary (citable) accession number: Q9P2N2
Secondary accession number(s): A8MQB7
, A8MU88, Q6P160, Q8N4T3, Q9NW53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: November 2, 2010
Last modified: July 22, 2015
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.