Reviewed,
UniProtKB/Swiss-Prot Q9P2M7 (CING_HUMAN)
Last modified
September 23, 2008.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Cingulin | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1197 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. |
| Subunit structure | Homodimer By similarity. Interacts with TJP1/ZO-1. |
| Subcellular location | Cell junction › tight junctionBy similarity. Note= Localizes to the apical junction complex composed of tight and adherens junctions By similarity. |
| Tissue specificity | Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart. |
| Domain | Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1. |
| Sequence similarities | Belongs to the cingulin family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Cell junction Tight junction |
| Domain | Coiled coil |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Cellular component | tight junction Non-traceable author statement. Source: UniProtKB |
| Molecular function | actin binding Inferred from sequence or structural similarity. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| TJP1 | Q07157 | 1 | EBI-79537,EBI-79553 | |
| Tjp1 | P39447 | 1 | EBI-79537,EBI-79508 | From a different organism. |
| YWHAB | P31946 | 1 | EBI-79537,EBI-359815 | |
| YWHAG | P61981 | 1 | EBI-79537,EBI-359832 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | ||||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1197 | 1197 | Cingulin | |||||
Regions | ||||||||
| Region | 1 – 351 | 351 | Head | |||||
| Region | 106 – 400 | 295 | Interacts with ZO-2 | |||||
| Region | 1155 – 1197 | 43 | Tail | |||||
| Coiled coil | 352 – 1154 | 803 | Potential | |||||
| Motif | 42 – 56 | 15 | ZIM | |||||
| Compositional bias | 363 – 836 | 474 | Glu-rich | |||||
Amino acid modifications | ||||||||
| Modified residue | 111 | 1 | Phosphoserine By similarity | |||||
| Modified residue | 112 | 1 | Phosphoserine By similarity | |||||
| Modified residue | 129 | 1 | Phosphoserine By similarity | |||||
| Modified residue | 131 | 1 | Phosphoserine | |||||
| Modified residue | 134 | 1 | Phosphoserine | |||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Human and Xenopus cingulin share a modular organization of the coiled-coil rod domain: predictions for intra- and intermolecular assembly." Citi S., D'Atri F., Parry D.A.D. J. Struct. Biol. 131:135-145(2000) [PubMed: 11042084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Neuroepithelium. |
| [2] | "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [3] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 368-376. Tissue: Platelet. |
| [5] | "Evidence for a functional interaction between cingulin and ZO-1 in cultured cells." D'Atri F., Nadalutti F., Citi S. J. Biol. Chem. 277:27757-27764(2002) [PubMed: 12023291] [Abstract] Cited for: INTERACTION WITH TJP1. |
| [6] | "Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134, MASS SPECTROMETRY. |
| [7] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| AF263462 mRNA. Translation: AAF74498.1. Different initiation. AB037740 mRNA. Translation: BAA92557.1. Different initiation. AL365436 Genomic DNA. Translation: CAI16590.1. Different initiation. | |
| RefSeq | NP_065821.1. |
| UniGene | Hs.591464 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1L4A based on UniProtKB O46345. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9P2M7. |
PTM databases | |
| PhosphoSite | Q9P2M7. |
Genome annotation databases | |
| Ensembl | ENSG00000143375. Homo sapiens. [Contig view] |
| GeneID | 57530. |
Organism-specific databases | |
| H-InvDB | HIX0001061. |
| HGNC | HGNC:17429. CGN. |
| HPA | CAB017193. |
| MIM | 609473. gene. |
| PharmGKB | PA134938123. |
| HUGE | Search... |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOVERGEN | Q9P2M7. |
Gene expression databases | |
| ArrayExpress | Q9P2M7. |
| CleanEx | HS_CGN. |
| GermOnline | ENSG00000143375. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR002928. Myosin_tail. [Graphical view] |
| Pfam | PF01576. Myosin_tail_1. 1 hit. [Graphical view] |
| ProDom | Q9P2M7. [Graphical view] [Entries sharing at least one domain] |
| BLOCKS | Search... |
Other Resources | |
| SOURCE | Search... |
| ProtoNet | Search... |
Entry information
| Entry name | CING_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9P2M7 Secondary accession number(s): Q5T386, Q9NR25 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


