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Protein

Cingulin

Gene

CGN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Caution

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cadherin binding Source: BHF-UCL
  • motor activity Source: InterPro

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)

Names & Taxonomyi

Protein namesi
Recommended name:
Cingulin
Gene namesi
Name:CGN
Synonyms:KIAA1319
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000143375.14
HGNCiHGNC:17429 CGN
MIMi609473 gene
neXtProtiNX_Q9P2M7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Tight junction

Pathology & Biotechi

Organism-specific databases

DisGeNETi57530
PharmGKBiPA134938123

Polymorphism and mutation databases

BioMutaiCGN
DMDMi27923755

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000897631 – 1197CingulinAdd BLAST1197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei129PhosphoserineCombined sources1
Modified residuei131PhosphoserineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei149PhosphoserineCombined sources1
Modified residuei159PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei211PhosphoserineCombined sources1
Modified residuei252PhosphoserineCombined sources1
Modified residuei270PhosphoserineCombined sources1
Modified residuei332PhosphoserineBy similarity1
Modified residuei345PhosphoserineBy similarity1
Modified residuei573N6-acetyllysineCombined sources1
Modified residuei706PhosphothreonineCombined sources1
Modified residuei1169PhosphoserineCombined sources1
Modified residuei1170PhosphoserineCombined sources1
Modified residuei1176PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9P2M7
MaxQBiQ9P2M7
PaxDbiQ9P2M7
PeptideAtlasiQ9P2M7
PRIDEiQ9P2M7

PTM databases

iPTMnetiQ9P2M7
PhosphoSitePlusiQ9P2M7

Expressioni

Tissue specificityi

Localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Expressed in pancreas, kidney, liver and lung, but not in skeletal muscle, placenta, brain or heart.

Gene expression databases

BgeeiENSG00000143375
CleanExiHS_CGN
ExpressionAtlasiQ9P2M7 baseline and differential
GenevisibleiQ9P2M7 HS

Organism-specific databases

HPAiCAB017193
HPA027586
HPA027587
HPA027657

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with TJP1/ZO-1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
YWHAGP619812EBI-79537,EBI-359832

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • cadherin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi121589103 interactors.
DIPiDIP-30947N
IntActiQ9P2M7 90 interactors.
MINTiQ9P2M7
STRINGi9606.ENSP00000271636

Structurei

3D structure databases

ProteinModelPortaliQ9P2M7
SMRiQ9P2M7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 351HeadAdd BLAST351
Regioni106 – 400Interaction with ZO-2Add BLAST295
Regioni1155 – 1197TailAdd BLAST43

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili352 – 1154Sequence analysisAdd BLAST803

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 56ZIMAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi363 – 836Glu-richAdd BLAST474

Domaini

Deletion of the ZO-1 interaction motif (ZIM) decreases but does not abolish colocalization with ZO-1.

Sequence similaritiesi

Belongs to the cingulin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGSJ Eukaryota
ENOG410YEC7 LUCA
HOVERGENiHBG031389
InParanoidiQ9P2M7
KOiK06102
OrthoDBiEOG091G012U
PhylomeDBiQ9P2M7
TreeFamiTF332247

Family and domain databases

InterProiView protein in InterPro
IPR002928 Myosin_tail
PfamiView protein in Pfam
PF01576 Myosin_tail_1, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2M7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEPRGPVDH GVQIRFITEP VSGAEMGTLR RGGRRPAKDA RASTYGVAVR
60 70 80 90 100
VQGIAGQPFV VLNSGEKGGD SFGVQIKGAN DQGASGALSS DLELPENPYS
110 120 130 140 150
QVKGFPAPSQ SSTSDEEPGA YWNGKLLRSH SQASLAGPGP VDPSNRSNSM
160 170 180 190 200
LELAPKVASP GSTIDTAPLS SVDSLINKFD SQLGGQARGR TGRRTRMLPP
210 220 230 240 250
EQRKRSKSLD SRLPRDTFEE RERQSTNHWT SSTKYDNHVG TSKQPAQSQN
260 270 280 290 300
LSPLSGFSRS RQTQDWVLQS FEEPRRSAQD PTMLQFKSTP DLLRDQQEAA
310 320 330 340 350
PPGSVDHMKA TIYGILREGS SESETSVRRK VSLVLEKMQP LVMVSSGSTK
360 370 380 390 400
AVAGQGELTR KVEELQRKLD EEVKKRQKLE PSQVGLERQL EEKTEECSRL
410 420 430 440 450
QELLERRKGE AQQSNKELQN MKRLLDQGED LRHGLETQVM ELQNKLKHVQ
460 470 480 490 500
GPEPAKEVLL KDLLETRELL EEVLEGKQRV EEQLRLRERE LTALKGALKE
510 520 530 540 550
EVASRDQEVE HVRQQYQRDT EQLRRSMQDA TQDHAVLEAE RQKMSALVRG
560 570 580 590 600
LQRELEETSE ETGHWQSMFQ KNKEDLRATK QELLQLRMEK EEMEEELGEK
610 620 630 640 650
IEVLQRELEQ ARASAGDTRQ VEVLKKELLR TQEELKELQA ERQSQEVAGR
660 670 680 690 700
HRDRELEKQL AVLRVEADRG RELEEQNLQL QKTLQQLRQD CEEASKAKMV
710 720 730 740 750
AEAEATVLGQ RRAAVETTLR ETQEENDEFR RRILGLEQQL KETRGLVDGG
760 770 780 790 800
EAVEARLRDK LQRLEAEKQQ LEEALNASQE EEGSLAAAKR ALEARLEEAQ
810 820 830 840 850
RGLARLGQEQ QTLNRALEEE GKQREVLRRG KAELEEQKRL LDRTVDRLNK
860 870 880 890 900
ELEKIGEDSK QALQQLQAQL EDYKEKARRE VADAQRQAKD WASEAEKTSG
910 920 930 940 950
GLSRLQDEIQ RLRQALQASQ AERDTARLDK ELLAQRLQGL EQEAENKKRS
960 970 980 990 1000
QDDRARQLKG LEEKVSRLET ELDEEKNTVE LLTDRVNRGR DQVDQLRTEL
1010 1020 1030 1040 1050
MQERSARQDL ECDKISLERQ NKDLKTRLAS SEGFQKPSAS LSQLESQNQL
1060 1070 1080 1090 1100
LQERLQAEER EKTVLQSTNR KLERKVKELS IQIEDERQHV NDQKDQLSLR
1110 1120 1130 1140 1150
VKALKRQVDE AEEEIERLDG LRKKAQREVE EQHEVNEQLQ ARIKSLEKDS
1160 1170 1180 1190
WRKASRSAAE SALKNEGLSS DEEFDSVYDP SSIASLLTES NLQTSSC
Length:1,197
Mass (Da):136,386
Last modified:January 27, 2003 - v2
Checksum:i0C9375283ABAAF3D
GO
Isoform 2 (identifier: Q9P2M7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     698-771: KMVAEAEATV...QRLEAEKQQL → RGVGTGLRRW...KRLAGGSCSL
     772-1197: Missing.

Note: May be due to an intron retention.
Show »
Length:771
Mass (Da):87,047
Checksum:iF289EA3694E4CEC1
GO

Sequence cautioni

The sequence AAF74498 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI52446 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA92557 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAF82696 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI16590 differs from that shown. Reason: Erroneous initiation.Curated
The sequence EAW53434 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057809479R → Q. Corresponds to variant dbSNP:rs12038198Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037039698 – 771KMVAE…EKQQL → RGVGTGLRRWRLRVSGGLRS QRVWKVTCHGYVLTSSWVLG MWFKEARIWQGEDTCICVGV GFSEKRLAGGSCSL in isoform 2. 1 PublicationAdd BLAST74
Alternative sequenceiVSP_037040772 – 1197Missing in isoform 2. 1 PublicationAdd BLAST426

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263462 mRNA Translation: AAF74498.1 Different initiation.
AB037740 mRNA Translation: BAA92557.1 Different initiation.
AK290007 mRNA Translation: BAF82696.1 Different initiation.
AL365436 Genomic DNA Translation: CAI16590.1 Different initiation.
CH471121 Genomic DNA Translation: EAW53434.1 Different initiation.
BC146657 mRNA Translation: AAI46658.1
BC152445 mRNA Translation: AAI52446.1 Different initiation.
RefSeqiNP_065821.1, NM_020770.2
XP_005245422.1, XM_005245365.4
UniGeneiHs.591464

Genome annotation databases

EnsembliENST00000271636; ENSP00000271636; ENSG00000143375
GeneIDi57530
KEGGihsa:57530
UCSCiuc009wmw.4 human [Q9P2M7-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCING_HUMAN
AccessioniPrimary (citable) accession number: Q9P2M7
Secondary accession number(s): A6H8L3
, A7MD22, Q5T386, Q9NR25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: January 27, 2003
Last modified: March 28, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome