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Protein

TBC1 domain family member 14

Gene

TBC1D14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of starvation-induced autophagosome formation.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: GO_Central
  • protein kinase binding Source: BHF-UCL
  • Rab GTPase binding Source: GO_Central

GO - Biological processi

  • activation of GTPase activity Source: GO_Central
  • intracellular protein transport Source: GO_Central
  • negative regulation of autophagy Source: BHF-UCL
  • recycling endosome to Golgi transport Source: UniProtKB
  • regulation of autophagosome assembly Source: BHF-UCL
  • regulation of vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 14
Gene namesi
Name:TBC1D14
Synonyms:KIAA1322
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:29246. TBC1D14.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: BHF-UCL
  • cytosol Source: GOC
  • Golgi apparatus Source: UniProtKB-SubCell
  • recycling endosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi472R → A: Loss of inhibition of autophagosome formation; when associated with A-508. 1 Publication1
Mutagenesisi508Q → A: Loss of inhibition of autophagosome formation; when associated with A-472. 1 Publication1

Organism-specific databases

DisGeNETi57533.
OpenTargetsiENSG00000132405.
PharmGKBiPA128394697.

Polymorphism and mutation databases

BioMutaiTBC1D14.
DMDMi172044690.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002080401 – 693TBC1 domain family member 14Add BLAST693

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei91PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P2M4.
MaxQBiQ9P2M4.
PaxDbiQ9P2M4.
PeptideAtlasiQ9P2M4.
PRIDEiQ9P2M4.

PTM databases

iPTMnetiQ9P2M4.
PhosphoSitePlusiQ9P2M4.

Expressioni

Gene expression databases

BgeeiENSG00000132405.
CleanExiHS_TBC1D14.
ExpressionAtlasiQ9P2M4. baseline and differential.
GenevisibleiQ9P2M4. HS.

Organism-specific databases

HPAiHPA036930.
HPA036931.
HPA067398.

Interactioni

Subunit structurei

Interacts with ULK1. May interact with RAB11A and RAB11B, but does not exhibit any GTPase-activating activity toward these proteins.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RAB11BQ159072EBI-2797718,EBI-722234
ULK1O753854EBI-2797718,EBI-908831

GO - Molecular functioni

  • protein kinase binding Source: BHF-UCL
  • Rab GTPase binding Source: GO_Central

Protein-protein interaction databases

BioGridi121592. 25 interactors.
IntActiQ9P2M4. 15 interactors.
MINTiMINT-4777056.
STRINGi9606.ENSP00000386921.

Structurei

Secondary structure

1693
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi373 – 379Combined sources7
Helixi382 – 384Combined sources3
Helixi392 – 399Combined sources8
Helixi404 – 415Combined sources12
Helixi423 – 436Combined sources14
Helixi462 – 471Combined sources10
Helixi475 – 477Combined sources3
Beta strandi479 – 481Combined sources3
Helixi487 – 500Combined sources14
Turni502 – 504Combined sources3
Helixi510 – 520Combined sources11
Helixi523 – 534Combined sources12
Helixi537 – 543Combined sources7
Helixi548 – 564Combined sources17
Helixi566 – 574Combined sources9
Helixi579 – 581Combined sources3
Helixi583 – 588Combined sources6
Turni589 – 594Combined sources6
Helixi597 – 610Combined sources14
Helixi612 – 625Combined sources14
Helixi627 – 631Combined sources5
Helixi635 – 643Combined sources9
Helixi651 – 660Combined sources10
Helixi670 – 678Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QQ8X-ray2.00A372-687[»]
ProteinModelPortaliQ9P2M4.
SMRiQ9P2M4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9P2M4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini401 – 611Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST211

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2223. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00750000117238.
HOGENOMiHOG000016604.
HOVERGENiHBG061665.
InParanoidiQ9P2M4.
KOiK20167.
OMAiVQMQSRN.
OrthoDBiEOG091G09QI.
PhylomeDBiQ9P2M4.
TreeFamiTF313318.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2M4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDGKLSTST NGVAFMGILD GRPGNPLQNL QHVNLKAPRL LSAPEYGPKL
60 70 80 90 100
KLRALEDRHS LQSVDSGIPT LEIGNPEPVP CSAVHVRRKQ SDSDLIPERA
110 120 130 140 150
FQSACALPSC APPAPSSTER EQSVRKSSTF PRTGYDSVKL YSPTSKALTR
160 170 180 190 200
SDDVSVCSVS SLGTELSTTL SVSNEDILDL VVTSSSSAIV TLENDDDPQF
210 220 230 240 250
TNVTLSSIKE TRGLHQQDCV HEAEEGSKLK ILGPFSNFFA RNLLARKQSA
260 270 280 290 300
RLDKHNDLGW KLFGKAPLRE NAQKDSKRIQ KEYEDKAGRP SKPPSPKQNV
310 320 330 340 350
RKNLDFEPLS TTALILEDRP ANLPAKPAEE AQKHRQQYEE MVVQAKKREL
360 370 380 390 400
KEAQRRKKQL EERCRVEESI GNAVLTWNNE ILPNWETMWC SRKVRDLWWQ
410 420 430 440 450
GIPPSVRGKV WSLAIGNELN ITHELFDICL ARAKERWRSL STGGSEVENE
460 470 480 490 500
DAGFSAADRE ASLELIKLDI SRTFPNLCIF QQGGPYHDML HSILGAYTCY
510 520 530 540 550
RPDVGYVQGM SFIAAVLILN LDTADAFIAF SNLLNKPCQM AFFRVDHGLM
560 570 580 590 600
LTYFAAFEVF FEENLPKLFA HFKKNNLTPD IYLIDWIFTL YSKSLPLDLA
610 620 630 640 650
CRIWDVFCRD GEEFLFRTAL GILKLFEDIL TKMDFIHMAQ FLTRLPEDLP
660 670 680 690
AEELFASIAT IQMQSRNKKW AQVLTALQKD SREMEKGSPS LRH
Length:693
Mass (Da):78,137
Last modified:February 26, 2008 - v3
Checksum:i6E1332D236DE45BD
GO
Isoform 2 (identifier: Q9P2M4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-280: Missing.
     281-281: K → M

Note: No experimental confirmation available.
Show »
Length:413
Mass (Da):47,691
Checksum:iE3BF0E3B0730079C
GO

Sequence cautioni

The sequence AAH41167 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA92560 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06744241L → V.2 PublicationsCorresponds to variant rs34860182dbSNPEnsembl.1
Natural variantiVAR_059856446E → Q.Corresponds to variant rs11731231dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0414601 – 280Missing in isoform 2. CuratedAdd BLAST280
Alternative sequenceiVSP_041461281K → M in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449900 mRNA. Translation: BAH16643.1.
AB037743 mRNA. Translation: BAA92560.1. Different initiation.
AC092463 Genomic DNA. No translation available.
AC097382 Genomic DNA. No translation available.
AC106045 Genomic DNA. No translation available.
CH471131 Genomic DNA. Translation: EAW82375.1.
CH471131 Genomic DNA. Translation: EAW82376.1.
CH471131 Genomic DNA. Translation: EAW82377.1.
BC041167 mRNA. Translation: AAH41167.1. Different initiation.
AL833868 mRNA. Translation: CAD38726.1.
CCDSiCCDS3394.2. [Q9P2M4-1]
CCDS47006.1. [Q9P2M4-2]
RefSeqiNP_001106832.1. NM_001113361.1. [Q9P2M4-1]
NP_001106834.1. NM_001113363.1. [Q9P2M4-2]
NP_001273734.1. NM_001286805.1.
NP_001317567.1. NM_001330638.1.
NP_065824.2. NM_020773.2. [Q9P2M4-1]
XP_006713958.1. XM_006713895.2. [Q9P2M4-1]
UniGeneiHs.518611.

Genome annotation databases

EnsembliENST00000409757; ENSP00000386921; ENSG00000132405. [Q9P2M4-1]
ENST00000448507; ENSP00000404041; ENSG00000132405. [Q9P2M4-1]
ENST00000451522; ENSP00000388886; ENSG00000132405. [Q9P2M4-2]
GeneIDi57533.
KEGGihsa:57533.
UCSCiuc003gjs.5. human. [Q9P2M4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449900 mRNA. Translation: BAH16643.1.
AB037743 mRNA. Translation: BAA92560.1. Different initiation.
AC092463 Genomic DNA. No translation available.
AC097382 Genomic DNA. No translation available.
AC106045 Genomic DNA. No translation available.
CH471131 Genomic DNA. Translation: EAW82375.1.
CH471131 Genomic DNA. Translation: EAW82376.1.
CH471131 Genomic DNA. Translation: EAW82377.1.
BC041167 mRNA. Translation: AAH41167.1. Different initiation.
AL833868 mRNA. Translation: CAD38726.1.
CCDSiCCDS3394.2. [Q9P2M4-1]
CCDS47006.1. [Q9P2M4-2]
RefSeqiNP_001106832.1. NM_001113361.1. [Q9P2M4-1]
NP_001106834.1. NM_001113363.1. [Q9P2M4-2]
NP_001273734.1. NM_001286805.1.
NP_001317567.1. NM_001330638.1.
NP_065824.2. NM_020773.2. [Q9P2M4-1]
XP_006713958.1. XM_006713895.2. [Q9P2M4-1]
UniGeneiHs.518611.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QQ8X-ray2.00A372-687[»]
ProteinModelPortaliQ9P2M4.
SMRiQ9P2M4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121592. 25 interactors.
IntActiQ9P2M4. 15 interactors.
MINTiMINT-4777056.
STRINGi9606.ENSP00000386921.

PTM databases

iPTMnetiQ9P2M4.
PhosphoSitePlusiQ9P2M4.

Polymorphism and mutation databases

BioMutaiTBC1D14.
DMDMi172044690.

Proteomic databases

EPDiQ9P2M4.
MaxQBiQ9P2M4.
PaxDbiQ9P2M4.
PeptideAtlasiQ9P2M4.
PRIDEiQ9P2M4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409757; ENSP00000386921; ENSG00000132405. [Q9P2M4-1]
ENST00000448507; ENSP00000404041; ENSG00000132405. [Q9P2M4-1]
ENST00000451522; ENSP00000388886; ENSG00000132405. [Q9P2M4-2]
GeneIDi57533.
KEGGihsa:57533.
UCSCiuc003gjs.5. human. [Q9P2M4-1]

Organism-specific databases

CTDi57533.
DisGeNETi57533.
GeneCardsiTBC1D14.
HGNCiHGNC:29246. TBC1D14.
HPAiHPA036930.
HPA036931.
HPA067398.
MIMi614855. gene.
neXtProtiNX_Q9P2M4.
OpenTargetsiENSG00000132405.
PharmGKBiPA128394697.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2223. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00750000117238.
HOGENOMiHOG000016604.
HOVERGENiHBG061665.
InParanoidiQ9P2M4.
KOiK20167.
OMAiVQMQSRN.
OrthoDBiEOG091G09QI.
PhylomeDBiQ9P2M4.
TreeFamiTF313318.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132405-MONOMER.

Miscellaneous databases

ChiTaRSiTBC1D14. human.
EvolutionaryTraceiQ9P2M4.
GenomeRNAii57533.
PROiQ9P2M4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132405.
CleanExiHS_TBC1D14.
ExpressionAtlasiQ9P2M4. baseline and differential.
GenevisibleiQ9P2M4. HS.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTBC14_HUMAN
AccessioniPrimary (citable) accession number: Q9P2M4
Secondary accession number(s): B9A6L5
, D3DVT4, E9PAZ6, Q8IW15, Q8NDK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: February 26, 2008
Last modified: November 30, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.