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Q9P2J3 (KLHL9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kelch-like protein 9
Gene names
Name:KLHL9
Synonyms:KIAA1354
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length617 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Ref.3 Ref.4 Ref.5

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts with AURKB. Ref.3 Ref.4

Sequence similarities

Contains 1 BACK (BTB/Kelch associated) domain.

Contains 1 BTB (POZ) domain.

Contains 6 Kelch repeats.

Sequence caution

The sequence BAA92592.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 617617Kelch-like protein 9
PRO_0000119110

Regions

Domain50 – 11970BTB
Domain154 – 255102BACK
Repeat300 – 34748Kelch 1
Repeat348 – 39952Kelch 2
Repeat400 – 44647Kelch 3
Repeat448 – 49346Kelch 4
Repeat495 – 54551Kelch 5
Repeat546 – 59550Kelch 6

Sequences

Sequence LengthMass (Da)Tools
Q9P2J3 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: B74232F28ABF7C82

FASTA61769,429
        10         20         30         40         50         60 
MKVSLGNGEM GVSAHLQPCK AGTTRFFTSN THSSVVLQGF DQLRIEGLLC DVTLVPGDGD 

        70         80         90        100        110        120 
EIFPVHRAMM ASASDYFKAM FTGGMKEQDL MCIKLHGVNK VGLKKIIDFI YTAKLSLNMD 

       130        140        150        160        170        180 
NLQDTLEAAS FLQILPVLDF CKVFLISGVS LDNCVEVGRI ANTYNLIEVD KYVNNFILKN 

       190        200        210        220        230        240 
FPALLSTGEF LKLPFERLAF VLSSNSLKHC TELELFKAAC RWLRLEDPRM DYAAKLMKNI 

       250        260        270        280        290        300 
RFPLMTPQDL INYVQTVDFM RTDNTCVNLL LEASNYQMMP YMQPVMQSDR TAIRSDSTHL 

       310        320        330        340        350        360 
VTLGGVLRQQ LVVSKELRMY DERAQEWRSL APMDAPRYQH GIAVIGNFLY VVGGQSNYDT 

       370        380        390        400        410        420 
KGKTAVDTVF RFDPRYNKWM QVASLNEKRT FFHLSALKGH LYAVGGRSAA GELATVECYN 

       430        440        450        460        470        480 
PRMNEWSYVA KMSEPHYGHA GTVYGGLMYI SGGITHDTFQ NELMCFDPDT DKWMQKAPMT 

       490        500        510        520        530        540 
TVRGLHCMCT VGDKLYVIGG NHFRGTSDYD DVLSCEYYSP TLDQWTPIAA MLRGQSDVGV 

       550        560        570        580        590        600 
AVFENKIYVV GGYSWNNRCM VEIVQKYDPE KDEWHKVFDL PESLGGIRAC TLTVFPPEEN 

       610 
PGSPSRESPL SAPSDHS 

« Hide

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Amygdala.
[3]"Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases."
Furukawa M., He Y.J., Borchers C., Xiong Y.
Nat. Cell Biol. 5:1001-1007(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN E3 UBIQUITIN-PROTEIN LIGASE, INTERACTION WITH CUL3.
[4]"A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells."
Sumara I., Quadroni M., Frei C., Olma M.H., Sumara G., Ricci R., Peter M.
Dev. Cell 12:887-900(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH AURKB; CUL3 AND KLHL13.
[5]"The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis."
Maerki S., Olma M.H., Staubli T., Steigemann P., Gerlich D.W., Quadroni M., Sumara I., Peter M.
J. Cell Biol. 187:791-800(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB037775 mRNA. Translation: BAA92592.1. Different initiation.
AL713669 mRNA. Translation: CAD28475.1.
RefSeqNP_061335.1. NM_018847.2.
UniGeneHs.701778.
Hs.710126.
Hs.744736.

3D structure databases

ProteinModelPortalQ9P2J3.
SMRQ9P2J3. Positions 27-273, 300-592.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121009. 13 interactions.
IntActQ9P2J3. 4 interactions.
MINTMINT-1209163.
STRING9606.ENSP00000351933.

PTM databases

PhosphoSiteQ9P2J3.

Polymorphism databases

DMDM51338828.

Proteomic databases

PaxDbQ9P2J3.
PRIDEQ9P2J3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000359039; ENSP00000351933; ENSG00000198642.
GeneID55958.
KEGGhsa:55958.
UCSCuc003zoy.3. human.

Organism-specific databases

CTD55958.
GeneCardsGC09M021329.
HGNCHGNC:18732. KLHL9.
HPAHPA046039.
MIM611201. gene.
neXtProtNX_Q9P2J3.
PharmGKBPA38662.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293088.
HOGENOMHOG000231583.
HOVERGENHBG106526.
InParanoidQ9P2J3.
KOK10447.
OMACEYYSPA.
OrthoDBEOG7VTDMJ.
PhylomeDBQ9P2J3.
TreeFamTF328485.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.
UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9P2J3.
BgeeQ9P2J3.
CleanExHS_KLHL9.
GenevestigatorQ9P2J3.

Family and domain databases

Gene3D2.130.10.80. 1 hit.
3.30.710.10. 1 hit.
InterProIPR011705. BACK.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR015916. Gal_Oxidase_b-propeller.
IPR017096. Kelch-like_gigaxonin-typ.
IPR006652. Kelch_1.
[Graphical view]
PfamPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 5 hits.
[Graphical view]
PIRSFPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi55958.
NextBio61363.
PROQ9P2J3.
SOURCESearch...

Entry information

Entry nameKLHL9_HUMAN
AccessionPrimary (citable) accession number: Q9P2J3
Secondary accession number(s): Q8TCQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM