Reviewed, UniProtKB/Swiss-Prot Q9P2I0 (CPSF2_HUMAN)

Names and origin

Protein names

Recommended name:
    Cleavage and polyadenylation specificity factor subunit 2
Alternative name(s):
    Cleavage and polyadenylation specificity factor 100 kDa subunit
      Short name=CPSF 100 kDa subunit

Gene names

Name:	CPSF2
Synonyms:	CPSF100, KIAA1367

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Ref.4
Subunit structure	Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from Experiment. Source: Reactome
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RNPS1	Q15287	1	EBI-1043224,EBI-395959

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 782	782	Cleavage and polyadenylation specificity factor subunit 2		PRO_0000074393
Amino acid modifications
	Modified residue	395	1	Phosphotyrosine Ref.6
	Modified residue	412	1	Phosphoserine By similarity
	Modified residue	419	1	Phosphoserine Ref.6 Ref.7
	Modified residue	420	1	Phosphoserine Ref.6 Ref.7
	Modified residue	423	1	Phosphoserine Ref.6 Ref.7
	Modified residue	660	1	Phosphoserine Ref.7 Ref.5
Experimental info
	Sequence conflict	289	1	D → G in AAH70095. Ref.1
	Sequence conflict	654	1	K → R in AAH70095. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9P2I0-1 [UniParc]. Last modified August 16, 2004. Version 2. Checksum: F67B4813B9883CE8	FASTA	782	88,487
	10 20 30 40 50 60 MTSIIKLTTL SGVQEESALC YLLQVDEFRF LLDCGWDEHF SMDIIDSLRK HVHQIDAVLL 70 80 90 100 110 120 SHPDPLHLGA LPYAVGKLGL NCAIYATIPV YKMGQMFMYD LYQSRHNTED FTLFTLDDVD 130 140 150 160 170 180 AAFDKIQQLK FSQIVNLKGK GHGLSITPLP AGHMIGGTIW KIVKDGEEEI VYAVDFNHKR 190 200 210 220 230 240 EIHLNGCSLE MLSRPSLLIT DSFNATYVQP RRKQRDEQLL TNVLETLRGD GNVLIAVDTA 250 260 270 280 290 300 GRVLELAQLL DQIWRTKDAG LGVYSLALLN NVSYNVVEFS KSQVEWMSDK LMRCFEDKRN 310 320 330 340 350 360 NPFQFRHLSL CHGLSDLARV PSPKVVLASQ PDLECGFSRD LFIQWCQDPK NSIILTYRTT 370 380 390 400 410 420 PGTLARFLID NPSEKITEIE LRKRVKLEGK ELEEYLEKEK LKKEAAKKLE QSKEADIDSS 430 440 450 460 470 480 DESDIEEDID QPSAHKTKHD LMMKGEGSRK GSFFKQAKKS YPMFPAPEER IKWDEYGEII 490 500 510 520 530 540 KPEDFLVPEL QATEEEKSKL ESGLTNGDEP MDQDLSDVPT KCISTTESIE IKARVTYIDY 550 560 570 580 590 600 EGRSDGDSIK KIINQMKPRQ LIIVHGPPEA SQDLAECCRA FGGKDIKVYM PKLHETVDAT 610 620 630 640 650 660 SETHIYQVRL KDSLVSSLQF CKAKDAELAW IDGVLDMRVS KVDTGVILEE GELKDDGEDS 670 680 690 700 710 720 EMQVEAPSDS SVIAQQKAMK SLFGDDEKET GEESEIIPTL EPLPPHEVPG HQSVFMNEPR 730 740 750 760 770 780 LSDFKQVLLR EGIQAEFVGG VLVCNNQVAV RRTETGRIGL EGCLCQDFYR IRDLLYEQYA IV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[2]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-782. Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 279-782. Tissue: Lymph node.
[4]	"Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase." Kaufmann I., Martin G., Friedlein A., Langen H., Keller W. EMBO J. 23:616-626(2004) [PubMed: 14749727] [Abstract] Cited for: FUNCTION IN PRE-MRNA 3'-END PROCESSING, IDENTIFICATION IN THE CPSF COMPLEX.
[5]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-660, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-395; SER-419; SER-420 AND SER-423, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419; SER-420; SER-423 AND SER-660, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC070095 mRNA. Translation: AAH70095.1. AB037788 mRNA. Translation: BAA92605.1. AL442079 mRNA. Translation: CAC09445.1.
IPI	IPI00419531.
RefSeq	NP_059133.1.
UniGene	Hs.657632
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P2I0. 1 interaction.
PTM databases
PhosphoSite	Q9P2I0.
Proteomic databases
PeptideAtlas	Q9P2I0.
PRIDE	Q9P2I0.
Genome annotation databases
Ensembl	ENSG00000165934. Homo sapiens. [Contig view]
GeneID	53981.
KEGG	hsa:53981.
UCSC	uc001yah.1. human.
Organism-specific databases
GeneCards	GC14P091658.
H-InvDB	HIX0020194.
HGNC	HGNC:2325. CPSF2.
HPA	HPA000870.
MIM	606028. gene.
PharmGKB	PA26842.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9P2I0.
HOVERGEN	Q9P2I0.
OMA	Q9P2I0. DYYKIRE.
Enzyme and pathway databases
Reactome	REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q9P2I0.
Bgee	Q9P2I0.
CleanEx	HS_CPSF2.
GermOnline	ENSG00000165934. Homo sapiens.
Family and domain databases
InterPro	IPR001279. Blactmase-like. IPR011108. RMMBL. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. PF07521. RMMBL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	56268.
SOURCE	Search...

Entry information

Entry name

CPSF2_HUMAN

Accession

Primary (citable) accession number: Q9P2I0
Secondary accession number(s): Q6NSJ1, Q9H3W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	August 16, 2004
Last modified:	July 7, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)