Reviewed,
UniProtKB/Swiss-Prot Q9P2I0 (CPSF2_HUMAN)
Last modified
July 7, 2009.
Version 59.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cleavage and polyadenylation specificity factor subunit 2 Alternative name(s): Cleavage and polyadenylation specificity factor 100 kDa subunit Short name=CPSF 100 kDa subunit | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 782 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Ref.4 |
| Subunit structure | Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. |
| Subcellular location | Nucleus Potential. |
| Sequence similarities | Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing |
| Cellular component | Nucleus |
| Ligand | RNA-binding |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | nuclear mRNA splicing, via spliceosome Inferred from Experiment. Source: Reactome |
| Cellular component | cytoplasm Inferred from direct assay. Source: HPA nucleusInferred from direct assay. Source: HPA |
| Molecular function | RNA binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activityInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 782 | 782 | Cleavage and polyadenylation specificity factor subunit 2 | PRO_0000074393 | |||||
Amino acid modifications | |||||||||
| Modified residue | 395 | 1 | Phosphotyrosine Ref.6 | ||||||
| Modified residue | 412 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 419 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
| Modified residue | 420 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
| Modified residue | 423 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
| Modified residue | 660 | 1 | Phosphoserine Ref.7 Ref.5 | ||||||
Experimental info | |||||||||
| Sequence conflict | 289 | 1 | D → G in AAH70095. Ref.1 | ||||||
| Sequence conflict | 654 | 1 | K → R in AAH70095. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis. |
| [2] | "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-782. Tissue: Brain. |
| [3] | "The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 279-782. Tissue: Lymph node. |
| [4] | "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase." Kaufmann I., Martin G., Friedlein A., Langen H., Keller W. EMBO J. 23:616-626(2004) [PubMed: 14749727] [Abstract] Cited for: FUNCTION IN PRE-MRNA 3'-END PROCESSING, IDENTIFICATION IN THE CPSF COMPLEX. |
| [5] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-660, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-395; SER-419; SER-420 AND SER-423, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419; SER-420; SER-423 AND SER-660, MASS SPECTROMETRY. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| BC070095 mRNA. Translation: AAH70095.1. AB037788 mRNA. Translation: BAA92605.1. AL442079 mRNA. Translation: CAC09445.1. | |
| IPI | IPI00419531. |
| RefSeq | NP_059133.1. |
| UniGene | Hs.657632 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9P2I0. 1 interaction. |
PTM databases | |
| PhosphoSite | Q9P2I0. |
Proteomic databases | |
| PeptideAtlas | Q9P2I0. |
| PRIDE | Q9P2I0. |
Genome annotation databases | |
| Ensembl | ENSG00000165934. Homo sapiens. [Contig view] |
| GeneID | 53981. |
| KEGG | hsa:53981. |
| UCSC | uc001yah.1. human. |
Organism-specific databases | |
| GeneCards | GC14P091658. |
| H-InvDB | HIX0020194. |
| HGNC | HGNC:2325. CPSF2. |
| HPA | HPA000870. |
| MIM | 606028. gene. |
| PharmGKB | PA26842. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9P2I0. |
| HOVERGEN | Q9P2I0. |
| OMA | Q9P2I0. DYYKIRE. |
Enzyme and pathway databases | |
| Reactome | REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_1788. Transcription. REACT_71. Gene Expression. |
Gene expression databases | |
| ArrayExpress | Q9P2I0. |
| Bgee | Q9P2I0. |
| CleanEx | HS_CPSF2. |
| GermOnline | ENSG00000165934. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001279. Blactmase-like. IPR011108. RMMBL. [Graphical view] |
| Pfam | PF00753. Lactamase_B. 1 hit. PF07521. RMMBL. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 56268. |
| SOURCE | Search... |
Entry information
| Entry name | CPSF2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9P2I0 Secondary accession number(s): Q6NSJ1, Q9H3W7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


