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Q9P2H5

- UBP35_HUMAN

UniProt

Q9P2H5 - UBP35_HUMAN

Protein

Ubiquitin carboxyl-terminal hydrolase 35

Gene

USP35

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 3 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei450 – 4501NucleophilePROSITE-ProRule annotation
    Active sitei862 – 8621Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: FlyBase

    GO - Biological processi

    1. protein deubiquitination Source: FlyBase
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 35 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 35
    Ubiquitin thioesterase 35
    Ubiquitin-specific-processing protease 35
    Gene namesi
    Name:USP35
    Synonyms:KIAA1372, USP34
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:20061. USP35.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134883706.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10181018Ubiquitin carboxyl-terminal hydrolase 35PRO_0000080665Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei613 – 6131Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9P2H5.
    PaxDbiQ9P2H5.
    PRIDEiQ9P2H5.

    PTM databases

    PhosphoSiteiQ9P2H5.

    Expressioni

    Tissue specificityi

    Expressed in testis, pancreas and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ9P2H5.
    BgeeiQ9P2H5.
    CleanExiHS_USP34.
    HS_USP35.
    GenevestigatoriQ9P2H5.

    Organism-specific databases

    HPAiHPA055213.
    HPA058592.

    Interactioni

    Protein-protein interaction databases

    BioGridi121613. 1 interaction.
    STRINGi9606.ENSP00000263311.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P2H5.
    SMRiQ9P2H5. Positions 441-627, 763-926.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini441 – 926486USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000007432.
    HOVERGENiHBG060424.
    InParanoidiQ9P2H5.
    KOiK11854.
    OMAiEREQCLA.
    PhylomeDBiQ9P2H5.
    TreeFamiTF324529.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9P2H5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDKILEAVVT SSYPVSVKQG LVRRVLEAAR QPLEREQCLA LLALGARLYV     50
    GGAEELPRRV GCQLLHVAGR HHPDVFAEFF SARRVLRLLQ GGAGPPGPRA 100
    LACVQLGLQL LPEGPAADEV FALLRREVLR TVCERPGPAA CAQVARLLAR 150
    HPRCVPDGPH RLLFCQQLVR CLGRFRCPAE GEEGAVEFLE QAQQVSGLLA 200
    QLWRAQPAAI LPCLKELFAV ISCAEEEPPS SALASVVQHL PLELMDGVVR 250
    NLSNDDSVTD SQMLTAISRM IDWVSWPLGK NIDKWIIALL KGLAAVKKFS 300
    ILIEVSLTKI EKVFSKLLYP IVRGAALSVL KYMLLTFQHS HEAFHLLLPH 350
    IPPMVASLVK EDSNSGTSCL EQLAELVHCM VFRFPGFPDL YEPVMEAIKD 400
    LHVPNEDRIK QLLGQDAWTS QKSELAGFYP RLMAKSDTGK IGLINLGNTC 450
    YVNSILQALF MASDFRHCVL RLTENNSQPL MTKLQWLFGF LEHSQRPAIS 500
    PENFLSASWT PWFSPGTQQD CSEYLKYLLD RLHEEEKTGT RICQKLKQSS 550
    SPSPPEEPPA PSSTSVEKMF GGKIVTRICC LCCLNVSSRE EAFTDLSLAF 600
    PPPERCRRRR LGSVMRPTED ITARELPPPT SAQGPGRVGP RRQRKHCITE 650
    DTPPTSLYIE GLDSKEAGGQ SSQEERIERE EEGKEERTEK EEVGEEEEST 700
    RGEGEREKEE EVEEEEEKVE KETEKEAEQE KEEDSLGAGT HPDAAIPSGE 750
    RTCGSEGSRS VLDLVNYFLS PEKLTAENRY YCESCASLQD AEKVVELSQG 800
    PCYLILTLLR FSFDLRTMRR RKILDDVSIP LLLRLPLAGG RGQAYDLCSV 850
    VVHSGVSSES GHYYCYAREG AARPAASLGT ADRPEPENQW YLFNDTRVSF 900
    SSFESVSNVT SFFPKDTAYV LFYRQRPREG PEAELGSSRV RTEPTLHKDL 950
    MEAISKDNIL YLQEQEKEAR SRAAYISALP TSPHWGRGFD EDKDEDEGSP 1000
    GGCNPAGGNG GDFHRLVF 1018
    Length:1,018
    Mass (Da):113,405
    Last modified:November 30, 2010 - v3
    Checksum:i79DECFF920B3666A
    GO
    Isoform 2 (identifier: Q9P2H5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-269: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:749
    Mass (Da):84,189
    Checksum:iF080EE8AB20A2109
    GO

    Sequence cautioni

    The sequence BAA92610.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti224 – 2241Missing in CAE51937. (PubMed:14715245)Curated
    Sequence conflicti464 – 4641D → E in CAE51937. (PubMed:14715245)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti236 – 2361V → M.
    Corresponds to variant rs2510044 [ dbSNP | Ensembl ].
    VAR_057042

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 269269Missing in isoform 2. 1 PublicationVSP_040291Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ586137 mRNA. Translation: CAE51937.1.
    AB037793 mRNA. Translation: BAA92610.1. Different initiation.
    AP002985 Genomic DNA. No translation available.
    CCDSiCCDS41693.1. [Q9P2H5-1]
    RefSeqiNP_065849.1. NM_020798.2. [Q9P2H5-1]
    UniGeneiHs.531249.

    Genome annotation databases

    EnsembliENST00000526425; ENSP00000434942; ENSG00000118369. [Q9P2H5-2]
    ENST00000529308; ENSP00000431876; ENSG00000118369. [Q9P2H5-1]
    GeneIDi57558.
    KEGGihsa:57558.
    UCSCiuc001ozc.3. human. [Q9P2H5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ586137 mRNA. Translation: CAE51937.1 .
    AB037793 mRNA. Translation: BAA92610.1 . Different initiation.
    AP002985 Genomic DNA. No translation available.
    CCDSi CCDS41693.1. [Q9P2H5-1 ]
    RefSeqi NP_065849.1. NM_020798.2. [Q9P2H5-1 ]
    UniGenei Hs.531249.

    3D structure databases

    ProteinModelPortali Q9P2H5.
    SMRi Q9P2H5. Positions 441-627, 763-926.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 121613. 1 interaction.
    STRINGi 9606.ENSP00000263311.

    Protein family/group databases

    MEROPSi C19.059.

    PTM databases

    PhosphoSitei Q9P2H5.

    Proteomic databases

    MaxQBi Q9P2H5.
    PaxDbi Q9P2H5.
    PRIDEi Q9P2H5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000526425 ; ENSP00000434942 ; ENSG00000118369 . [Q9P2H5-2 ]
    ENST00000529308 ; ENSP00000431876 ; ENSG00000118369 . [Q9P2H5-1 ]
    GeneIDi 57558.
    KEGGi hsa:57558.
    UCSCi uc001ozc.3. human. [Q9P2H5-1 ]

    Organism-specific databases

    CTDi 57558.
    GeneCardsi GC11P077899.
    H-InvDB HIX0009977.
    HGNCi HGNC:20061. USP35.
    HPAi HPA055213.
    HPA058592.
    neXtProti NX_Q9P2H5.
    PharmGKBi PA134883706.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000007432.
    HOVERGENi HBG060424.
    InParanoidi Q9P2H5.
    KOi K11854.
    OMAi EREQCLA.
    PhylomeDBi Q9P2H5.
    TreeFami TF324529.

    Miscellaneous databases

    ChiTaRSi USP35. human.
    GenomeRNAii 57558.
    NextBioi 64042.
    PROi Q9P2H5.

    Gene expression databases

    ArrayExpressi Q9P2H5.
    Bgeei Q9P2H5.
    CleanExi HS_USP34.
    HS_USP35.
    Genevestigatori Q9P2H5.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
      Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
      Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    2. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
      DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-613, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiUBP35_HUMAN
    AccessioniPrimary (citable) accession number: Q9P2H5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2003
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 103 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3