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Q9P2H5 (UBP35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 35

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 35
Ubiquitin thioesterase 35
Ubiquitin-specific-processing protease 35
Gene names
Name:USP35
Synonyms:KIAA1372, USP34
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1018 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.1

Tissue specificity

Expressed in testis, pancreas and skeletal muscle. Ref.1

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence BAA92610.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P2H5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P2H5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10181018Ubiquitin carboxyl-terminal hydrolase 35
PRO_0000080665

Regions

Domain441 – 926486USP

Sites

Active site4501Nucleophile By similarity
Active site8621Proton acceptor By similarity

Amino acid modifications

Modified residue6131Phosphoserine Ref.4

Natural variations

Alternative sequence1 – 269269Missing in isoform 2.
VSP_040291
Natural variant2361V → M.
Corresponds to variant rs2510044 [ dbSNP | Ensembl ].
VAR_057042

Experimental info

Sequence conflict2241Missing in CAE51937. Ref.1
Sequence conflict4641D → E in CAE51937. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 30, 2010. Version 3.
Checksum: 79DECFF920B3666A

FASTA1,018113,405
        10         20         30         40         50         60 
MDKILEAVVT SSYPVSVKQG LVRRVLEAAR QPLEREQCLA LLALGARLYV GGAEELPRRV 

        70         80         90        100        110        120 
GCQLLHVAGR HHPDVFAEFF SARRVLRLLQ GGAGPPGPRA LACVQLGLQL LPEGPAADEV 

       130        140        150        160        170        180 
FALLRREVLR TVCERPGPAA CAQVARLLAR HPRCVPDGPH RLLFCQQLVR CLGRFRCPAE 

       190        200        210        220        230        240 
GEEGAVEFLE QAQQVSGLLA QLWRAQPAAI LPCLKELFAV ISCAEEEPPS SALASVVQHL 

       250        260        270        280        290        300 
PLELMDGVVR NLSNDDSVTD SQMLTAISRM IDWVSWPLGK NIDKWIIALL KGLAAVKKFS 

       310        320        330        340        350        360 
ILIEVSLTKI EKVFSKLLYP IVRGAALSVL KYMLLTFQHS HEAFHLLLPH IPPMVASLVK 

       370        380        390        400        410        420 
EDSNSGTSCL EQLAELVHCM VFRFPGFPDL YEPVMEAIKD LHVPNEDRIK QLLGQDAWTS 

       430        440        450        460        470        480 
QKSELAGFYP RLMAKSDTGK IGLINLGNTC YVNSILQALF MASDFRHCVL RLTENNSQPL 

       490        500        510        520        530        540 
MTKLQWLFGF LEHSQRPAIS PENFLSASWT PWFSPGTQQD CSEYLKYLLD RLHEEEKTGT 

       550        560        570        580        590        600 
RICQKLKQSS SPSPPEEPPA PSSTSVEKMF GGKIVTRICC LCCLNVSSRE EAFTDLSLAF 

       610        620        630        640        650        660 
PPPERCRRRR LGSVMRPTED ITARELPPPT SAQGPGRVGP RRQRKHCITE DTPPTSLYIE 

       670        680        690        700        710        720 
GLDSKEAGGQ SSQEERIERE EEGKEERTEK EEVGEEEEST RGEGEREKEE EVEEEEEKVE 

       730        740        750        760        770        780 
KETEKEAEQE KEEDSLGAGT HPDAAIPSGE RTCGSEGSRS VLDLVNYFLS PEKLTAENRY 

       790        800        810        820        830        840 
YCESCASLQD AEKVVELSQG PCYLILTLLR FSFDLRTMRR RKILDDVSIP LLLRLPLAGG 

       850        860        870        880        890        900 
RGQAYDLCSV VVHSGVSSES GHYYCYAREG AARPAASLGT ADRPEPENQW YLFNDTRVSF 

       910        920        930        940        950        960 
SSFESVSNVT SFFPKDTAYV LFYRQRPREG PEAELGSSRV RTEPTLHKDL MEAISKDNIL 

       970        980        990       1000       1010 
YLQEQEKEAR SRAAYISALP TSPHWGRGFD EDKDEDEGSP GGCNPAGGNG GDFHRLVF 

« Hide

Isoform 2 [UniParc].

Checksum: F080EE8AB20A2109
Show »

FASTA74984,189

References

« Hide 'large scale' references
[1]"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[2]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-613, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ586137 mRNA. Translation: CAE51937.1.
AB037793 mRNA. Translation: BAA92610.1. Different initiation.
AP002985 Genomic DNA. No translation available.
RefSeqNP_065849.1. NM_020798.2.
XP_005274180.1. XM_005274123.1.
XP_005274181.1. XM_005274124.1.
UniGeneHs.531249.

3D structure databases

ProteinModelPortalQ9P2H5.
SMRQ9P2H5. Positions 441-627, 763-926.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121613. 1 interaction.
STRING9606.ENSP00000263311.

Protein family/group databases

MEROPSC19.059.

PTM databases

PhosphoSiteQ9P2H5.

Polymorphism databases

DMDM46577708.

Proteomic databases

PaxDbQ9P2H5.
PRIDEQ9P2H5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000526425; ENSP00000434942; ENSG00000118369. [Q9P2H5-2]
ENST00000529308; ENSP00000431876; ENSG00000118369. [Q9P2H5-1]
GeneID57558.
KEGGhsa:57558.
UCSCuc001ozc.3. human. [Q9P2H5-1]

Organism-specific databases

CTD57558.
GeneCardsGC11P077899.
H-InvDBHIX0009977.
HGNCHGNC:20061. USP35.
HPAHPA055213.
HPA058592.
neXtProtNX_Q9P2H5.
PharmGKBPA134883706.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5077.
HOGENOMHOG000007432.
HOVERGENHBG060424.
InParanoidQ9P2H5.
KOK11854.
OMAEREQCLA.
PhylomeDBQ9P2H5.
TreeFamTF324529.

Gene expression databases

ArrayExpressQ9P2H5.
BgeeQ9P2H5.
CleanExHS_USP34.
HS_USP35.
GenevestigatorQ9P2H5.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP35. human.
GenomeRNAi57558.
NextBio64042.
PROQ9P2H5.

Entry information

Entry nameUBP35_HUMAN
AccessionPrimary (citable) accession number: Q9P2H5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: November 30, 2010
Last modified: March 19, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM