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Q9P2H5

- UBP35_HUMAN

UniProt

Q9P2H5 - UBP35_HUMAN

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Protein

Ubiquitin carboxyl-terminal hydrolase 35

Gene

USP35

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei450 – 4501NucleophilePROSITE-ProRule annotation
Active sitei862 – 8621Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  1. protein deubiquitination Source: FlyBase
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.059.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 35 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 35
Ubiquitin thioesterase 35
Ubiquitin-specific-processing protease 35
Gene namesi
Name:USP35
Synonyms:KIAA1372, USP34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:20061. USP35.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134883706.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10181018Ubiquitin carboxyl-terminal hydrolase 35PRO_0000080665Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei613 – 6131Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P2H5.
PaxDbiQ9P2H5.
PRIDEiQ9P2H5.

PTM databases

PhosphoSiteiQ9P2H5.

Expressioni

Tissue specificityi

Expressed in testis, pancreas and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ9P2H5.
CleanExiHS_USP34.
HS_USP35.
ExpressionAtlasiQ9P2H5. baseline and differential.
GenevestigatoriQ9P2H5.

Organism-specific databases

HPAiHPA055213.
HPA058592.

Interactioni

Protein-protein interaction databases

BioGridi121613. 1 interaction.
STRINGi9606.ENSP00000263311.

Structurei

3D structure databases

ProteinModelPortaliQ9P2H5.
SMRiQ9P2H5. Positions 441-627, 763-926.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini441 – 926486USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000007432.
HOVERGENiHBG060424.
InParanoidiQ9P2H5.
KOiK11854.
OMAiEREQCLA.
PhylomeDBiQ9P2H5.
TreeFamiTF324529.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9P2H5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKILEAVVT SSYPVSVKQG LVRRVLEAAR QPLEREQCLA LLALGARLYV
60 70 80 90 100
GGAEELPRRV GCQLLHVAGR HHPDVFAEFF SARRVLRLLQ GGAGPPGPRA
110 120 130 140 150
LACVQLGLQL LPEGPAADEV FALLRREVLR TVCERPGPAA CAQVARLLAR
160 170 180 190 200
HPRCVPDGPH RLLFCQQLVR CLGRFRCPAE GEEGAVEFLE QAQQVSGLLA
210 220 230 240 250
QLWRAQPAAI LPCLKELFAV ISCAEEEPPS SALASVVQHL PLELMDGVVR
260 270 280 290 300
NLSNDDSVTD SQMLTAISRM IDWVSWPLGK NIDKWIIALL KGLAAVKKFS
310 320 330 340 350
ILIEVSLTKI EKVFSKLLYP IVRGAALSVL KYMLLTFQHS HEAFHLLLPH
360 370 380 390 400
IPPMVASLVK EDSNSGTSCL EQLAELVHCM VFRFPGFPDL YEPVMEAIKD
410 420 430 440 450
LHVPNEDRIK QLLGQDAWTS QKSELAGFYP RLMAKSDTGK IGLINLGNTC
460 470 480 490 500
YVNSILQALF MASDFRHCVL RLTENNSQPL MTKLQWLFGF LEHSQRPAIS
510 520 530 540 550
PENFLSASWT PWFSPGTQQD CSEYLKYLLD RLHEEEKTGT RICQKLKQSS
560 570 580 590 600
SPSPPEEPPA PSSTSVEKMF GGKIVTRICC LCCLNVSSRE EAFTDLSLAF
610 620 630 640 650
PPPERCRRRR LGSVMRPTED ITARELPPPT SAQGPGRVGP RRQRKHCITE
660 670 680 690 700
DTPPTSLYIE GLDSKEAGGQ SSQEERIERE EEGKEERTEK EEVGEEEEST
710 720 730 740 750
RGEGEREKEE EVEEEEEKVE KETEKEAEQE KEEDSLGAGT HPDAAIPSGE
760 770 780 790 800
RTCGSEGSRS VLDLVNYFLS PEKLTAENRY YCESCASLQD AEKVVELSQG
810 820 830 840 850
PCYLILTLLR FSFDLRTMRR RKILDDVSIP LLLRLPLAGG RGQAYDLCSV
860 870 880 890 900
VVHSGVSSES GHYYCYAREG AARPAASLGT ADRPEPENQW YLFNDTRVSF
910 920 930 940 950
SSFESVSNVT SFFPKDTAYV LFYRQRPREG PEAELGSSRV RTEPTLHKDL
960 970 980 990 1000
MEAISKDNIL YLQEQEKEAR SRAAYISALP TSPHWGRGFD EDKDEDEGSP
1010
GGCNPAGGNG GDFHRLVF
Length:1,018
Mass (Da):113,405
Last modified:November 30, 2010 - v3
Checksum:i79DECFF920B3666A
GO
Isoform 2 (identifier: Q9P2H5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.

Note: No experimental confirmation available.

Show »
Length:749
Mass (Da):84,189
Checksum:iF080EE8AB20A2109
GO

Sequence cautioni

The sequence BAA92610.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241Missing in CAE51937. (PubMed:14715245)Curated
Sequence conflicti464 – 4641D → E in CAE51937. (PubMed:14715245)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti236 – 2361V → M.
Corresponds to variant rs2510044 [ dbSNP | Ensembl ].
VAR_057042

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 269269Missing in isoform 2. 1 PublicationVSP_040291Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586137 mRNA. Translation: CAE51937.1.
AB037793 mRNA. Translation: BAA92610.1. Different initiation.
AP002985 Genomic DNA. No translation available.
CCDSiCCDS41693.1. [Q9P2H5-1]
RefSeqiNP_065849.1. NM_020798.2. [Q9P2H5-1]
UniGeneiHs.531249.

Genome annotation databases

EnsembliENST00000526425; ENSP00000434942; ENSG00000118369. [Q9P2H5-2]
ENST00000529308; ENSP00000431876; ENSG00000118369. [Q9P2H5-1]
GeneIDi57558.
KEGGihsa:57558.
UCSCiuc001ozc.3. human. [Q9P2H5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586137 mRNA. Translation: CAE51937.1 .
AB037793 mRNA. Translation: BAA92610.1 . Different initiation.
AP002985 Genomic DNA. No translation available.
CCDSi CCDS41693.1. [Q9P2H5-1 ]
RefSeqi NP_065849.1. NM_020798.2. [Q9P2H5-1 ]
UniGenei Hs.531249.

3D structure databases

ProteinModelPortali Q9P2H5.
SMRi Q9P2H5. Positions 441-627, 763-926.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121613. 1 interaction.
STRINGi 9606.ENSP00000263311.

Protein family/group databases

MEROPSi C19.059.

PTM databases

PhosphoSitei Q9P2H5.

Proteomic databases

MaxQBi Q9P2H5.
PaxDbi Q9P2H5.
PRIDEi Q9P2H5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000526425 ; ENSP00000434942 ; ENSG00000118369 . [Q9P2H5-2 ]
ENST00000529308 ; ENSP00000431876 ; ENSG00000118369 . [Q9P2H5-1 ]
GeneIDi 57558.
KEGGi hsa:57558.
UCSCi uc001ozc.3. human. [Q9P2H5-1 ]

Organism-specific databases

CTDi 57558.
GeneCardsi GC11P077899.
H-InvDB HIX0009977.
HGNCi HGNC:20061. USP35.
HPAi HPA055213.
HPA058592.
neXtProti NX_Q9P2H5.
PharmGKBi PA134883706.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00650000093027.
HOGENOMi HOG000007432.
HOVERGENi HBG060424.
InParanoidi Q9P2H5.
KOi K11854.
OMAi EREQCLA.
PhylomeDBi Q9P2H5.
TreeFami TF324529.

Miscellaneous databases

ChiTaRSi USP35. human.
GenomeRNAii 57558.
NextBioi 64042.
PROi Q9P2H5.

Gene expression databases

Bgeei Q9P2H5.
CleanExi HS_USP34.
HS_USP35.
ExpressionAtlasi Q9P2H5. baseline and differential.
Genevestigatori Q9P2H5.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
    Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
    Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  2. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-613, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiUBP35_HUMAN
AccessioniPrimary (citable) accession number: Q9P2H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: November 30, 2010
Last modified: October 29, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3