Q9P2H5 (UBP35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 35
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 35
Ubiquitin thioesterase 35
Ubiquitin-specific-processing protease 35

Gene names

Name:	USP35
Synonyms:	KIAA1372, USP34

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1018 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.1
Tissue specificity	Expressed in testis, pancreas and skeletal muscle. Ref.1
Sequence similarities	Belongs to the peptidase C19 family.
Sequence caution	The sequence BAA92610.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P2H5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P2H5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-269: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1018	1018	Ubiquitin carboxyl-terminal hydrolase 35		PRO_0000080665
Sites
	Active site	450	1	Nucleophile By similarity
	Active site	862	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	613	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	1 – 269	269	Missing in isoform 2.		VSP_040291
	Natural variant	236	1	V → M. Corresponds to variant rs2510044 [ dbSNP | Ensembl ].		VAR_057042
Experimental info
	Sequence conflict	224	1	Missing in CAE51937. Ref.1
	Sequence conflict	464	1	D → E in CAE51937. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: 79DECFF920B3666A	FASTA	1,018	113,405
	10 20 30 40 50 60 MDKILEAVVT SSYPVSVKQG LVRRVLEAAR QPLEREQCLA LLALGARLYV GGAEELPRRV 70 80 90 100 110 120 GCQLLHVAGR HHPDVFAEFF SARRVLRLLQ GGAGPPGPRA LACVQLGLQL LPEGPAADEV 130 140 150 160 170 180 FALLRREVLR TVCERPGPAA CAQVARLLAR HPRCVPDGPH RLLFCQQLVR CLGRFRCPAE 190 200 210 220 230 240 GEEGAVEFLE QAQQVSGLLA QLWRAQPAAI LPCLKELFAV ISCAEEEPPS SALASVVQHL 250 260 270 280 290 300 PLELMDGVVR NLSNDDSVTD SQMLTAISRM IDWVSWPLGK NIDKWIIALL KGLAAVKKFS 310 320 330 340 350 360 ILIEVSLTKI EKVFSKLLYP IVRGAALSVL KYMLLTFQHS HEAFHLLLPH IPPMVASLVK 370 380 390 400 410 420 EDSNSGTSCL EQLAELVHCM VFRFPGFPDL YEPVMEAIKD LHVPNEDRIK QLLGQDAWTS 430 440 450 460 470 480 QKSELAGFYP RLMAKSDTGK IGLINLGNTC YVNSILQALF MASDFRHCVL RLTENNSQPL 490 500 510 520 530 540 MTKLQWLFGF LEHSQRPAIS PENFLSASWT PWFSPGTQQD CSEYLKYLLD RLHEEEKTGT 550 560 570 580 590 600 RICQKLKQSS SPSPPEEPPA PSSTSVEKMF GGKIVTRICC LCCLNVSSRE EAFTDLSLAF 610 620 630 640 650 660 PPPERCRRRR LGSVMRPTED ITARELPPPT SAQGPGRVGP RRQRKHCITE DTPPTSLYIE 670 680 690 700 710 720 GLDSKEAGGQ SSQEERIERE EEGKEERTEK EEVGEEEEST RGEGEREKEE EVEEEEEKVE 730 740 750 760 770 780 KETEKEAEQE KEEDSLGAGT HPDAAIPSGE RTCGSEGSRS VLDLVNYFLS PEKLTAENRY 790 800 810 820 830 840 YCESCASLQD AEKVVELSQG PCYLILTLLR FSFDLRTMRR RKILDDVSIP LLLRLPLAGG 850 860 870 880 890 900 RGQAYDLCSV VVHSGVSSES GHYYCYAREG AARPAASLGT ADRPEPENQW YLFNDTRVSF 910 920 930 940 950 960 SSFESVSNVT SFFPKDTAYV LFYRQRPREG PEAELGSSRV RTEPTLHKDL MEAISKDNIL 970 980 990 1000 1010 YLQEQEKEAR SRAAYISALP TSPHWGRGFD EDKDEDEGSP GGCNPAGGNG GDFHRLVF
	Isoform 2 [UniParc]. Checksum: F080EE8AB20A2109	FASTA	749	84,189
	10 20 30 40 50 60 MIDWVSWPLG KNIDKWIIAL LKGLAAVKKF SILIEVSLTK IEKVFSKLLY PIVRGAALSV 70 80 90 100 110 120 LKYMLLTFQH SHEAFHLLLP HIPPMVASLV KEDSNSGTSC LEQLAELVHC MVFRFPGFPD 130 140 150 160 170 180 LYEPVMEAIK DLHVPNEDRI KQLLGQDAWT SQKSELAGFY PRLMAKSDTG KIGLINLGNT 190 200 210 220 230 240 CYVNSILQAL FMASDFRHCV LRLTENNSQP LMTKLQWLFG FLEHSQRPAI SPENFLSASW 250 260 270 280 290 300 TPWFSPGTQQ DCSEYLKYLL DRLHEEEKTG TRICQKLKQS SSPSPPEEPP APSSTSVEKM 310 320 330 340 350 360 FGGKIVTRIC CLCCLNVSSR EEAFTDLSLA FPPPERCRRR RLGSVMRPTE DITARELPPP 370 380 390 400 410 420 TSAQGPGRVG PRRQRKHCIT EDTPPTSLYI EGLDSKEAGG QSSQEERIER EEEGKEERTE 430 440 450 460 470 480 KEEVGEEEES TRGEGEREKE EEVEEEEEKV EKETEKEAEQ EKEEDSLGAG THPDAAIPSG 490 500 510 520 530 540 ERTCGSEGSR SVLDLVNYFL SPEKLTAENR YYCESCASLQ DAEKVVELSQ GPCYLILTLL 550 560 570 580 590 600 RFSFDLRTMR RRKILDDVSI PLLLRLPLAG GRGQAYDLCS VVVHSGVSSE SGHYYCYARE 610 620 630 640 650 660 GAARPAASLG TADRPEPENQ WYLFNDTRVS FSSFESVSNV TSFFPKDTAY VLFYRQRPRE 670 680 690 700 710 720 GPEAELGSSR VRTEPTLHKD LMEAISKDNI LYLQEQEKEA RSRAAYISAL PTSPHWGRGF 730 740 DEDKDEDEGS PGGCNPAGGN GGDFHRLVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases." Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C. Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[2]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-613, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ586137 mRNA. Translation: CAE51937.1. AB037793 mRNA. Translation: BAA92610.1. Different initiation. AP002985 Genomic DNA. No translation available.
IPI	IPI00001726. IPI00973101.
RefSeq	NP_065849.1. NM_020798.2.
UniGene	Hs.531249.
3D structure databases
ProteinModelPortal	Q9P2H5.
SMR	Q9P2H5. Positions 441-627, 763-926.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000263311.
Protein family/group databases
MEROPS	C19.059.
PTM databases
PhosphoSite	Q9P2H5.
Polymorphism databases
DMDM	46577708.
Proteomic databases
PaxDb	Q9P2H5.
PRIDE	Q9P2H5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000526425; ENSP00000434942; ENSG00000118369. ENST00000529308; ENSP00000431876; ENSG00000118369.
GeneID	57558.
KEGG	hsa:57558.
UCSC	uc001ozc.3. human.
Organism-specific databases
CTD	57558.
GeneCards	GC11P077899.
H-InvDB	HIX0009977.
HGNC	HGNC:20061. USP35.
neXtProt	NX_Q9P2H5.
PharmGKB	PA134883706.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000007432.
HOVERGEN	HBG060424.
InParanoid	Q9P2H5.
KO	K11854.
OMA	EREQCLA.
OrthoDB	EOG43XV2S.
PhylomeDB	Q9P2H5.
Gene expression databases
ArrayExpress	Q9P2H5.
Bgee	Q9P2H5.
CleanEx	HS_USP34. HS_USP35.
Genevestigator	Q9P2H5.
GermOnline	ENSG00000118369. Homo sapiens.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	USP35. human.
GenomeRNAi	57558.
NextBio	64042.

Entry information

Entry name

UBP35_HUMAN

Accession

Primary (citable) accession number: Q9P2H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.