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Q9P2G9 (KLHL8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kelch-like protein 8
Gene names
Name:KLHL8
Synonyms:KIAA1378
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length620 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN. Ref.6

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Component of the BCR(KLHL8) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL8 and RBX1. Interacts with RAPSN. Ref.6

Sequence similarities

Contains 1 BACK (BTB/Kelch associated) domain.

Contains 1 BTB (POZ) domain.

Contains 6 Kelch repeats.

Sequence caution

The sequence BAA92616.2 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 620619Kelch-like protein 8
PRO_0000119108

Regions

Domain67 – 13468BTB
Domain169 – 270102BACK
Repeat319 – 36648Kelch 1
Repeat367 – 41347Kelch 2
Repeat415 – 46046Kelch 3
Repeat462 – 50746Kelch 4
Repeat508 – 55447Kelch 5
Repeat556 – 60146Kelch 6

Amino acid modifications

Modified residue21N-acetylalanine Ref.7

Natural variations

Natural variant5201P → R. Ref.5
Corresponds to variant rs17854114 [ dbSNP | Ensembl ].
VAR_030012

Sequences

Sequence LengthMass (Da)Tools
Q9P2G9 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 6C3C3C78CEC6FE84

FASTA62068,802
        10         20         30         40         50         60 
MASDSMSSKQ ARNHITKGKR QQQHQQIKNR SSISDGDGED SFIFEANEAW KDFHGSLLRF 

        70         80         90        100        110        120 
YENGELCDVT LKVGSKLISC HKLVLACVIP YFRAMFLSEM AEAKQTLIEI RDFDGDAIED 

       130        140        150        160        170        180 
LVKFVYSSRL TLTVDNVQPL LYAACILQVE LVARACCEYM KLHFHPSNCL AVRAFAESHN 

       190        200        210        220        230        240 
RIDLMDMADQ YACDHFTEVV ECEDFVSVSP QHLHKLLSSS DLNIENEKQV YNAAIKWLLA 

       250        260        270        280        290        300 
NPQHHSKWLD ETLAQVRLPL LPVDFLMGVV AKEQIVKQNL KCRDLLDEAR NYHLHLSSRA 

       310        320        330        340        350        360 
VPDFEYSIRT TPRKHTAGVL FCVGGRGGSG DPFRSIECYS INKNSWFFGP EMNSRRRHVG 

       370        380        390        400        410        420 
VISVEGKVYA VGGHDGNEHL GSMEMFDPLT NKWMMKASMN TKRRGIALAS LGGPIYAIGG 

       430        440        450        460        470        480 
LDDNTCFNDV ERYDIESDQW STVAPMNTPR GGVGSVALVN HVYAVGGNDG MASLSSVERY 

       490        500        510        520        530        540 
DPHLDKWIEV KEMGQRRAGN GVSKLHGCLY VVGGFDDNSP LSSVERYDPR SNKWDYVAAL 

       550        560        570        580        590        600 
TTPRGGVGIA TVMGKIFAVG GHNGNAYLNT VEAFDPVLNR WELVGSVSHC RAGAGVAVCS 

       610        620 
CLTSQIRDVG HGSNNVVDCM 

« Hide

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[2]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Cerebellum.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-520.
Tissue: Brain.
[6]"Control of rapsyn stability by the CUL-3-containing E3 ligase complex."
Nam S., Min K., Hwang H., Lee H.O., Lee J.H., Yoon J., Lee H., Park S., Lee J.
J. Biol. Chem. 284:8195-8206(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN A BCR (BTB-CUL3-RBX1) E3 UBIQUITIN LIGASE COMPLEX, INTERACTION WITH RAPSN.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB037799 mRNA. Translation: BAA92616.2. Different initiation.
BX640727 mRNA. Translation: CAE45843.1.
CH471057 Genomic DNA. Translation: EAX05982.1.
BC041384 mRNA. Translation: AAH41384.1.
RefSeqNP_065854.3. NM_020803.3.
UniGeneHs.106601.

3D structure databases

ProteinModelPortalQ9P2G9.
SMRQ9P2G9. Positions 44-293, 319-598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121618. 9 interactions.
IntActQ9P2G9. 4 interactions.
STRING9606.ENSP00000273963.

PTM databases

PhosphoSiteQ9P2G9.

Polymorphism databases

DMDM124056473.

Proteomic databases

PaxDbQ9P2G9.
PRIDEQ9P2G9.

Protocols and materials databases

DNASU57563.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273963; ENSP00000273963; ENSG00000145332.
ENST00000512111; ENSP00000424131; ENSG00000145332.
GeneID57563.
KEGGhsa:57563.
UCSCuc003hql.1. human.

Organism-specific databases

CTD57563.
GeneCardsGC04M088081.
HGNCHGNC:18644. KLHL8.
HPAHPA013856.
HPA017762.
MIM611967. gene.
neXtProtNX_Q9P2G9.
PharmGKBPA38616.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG299517.
HOGENOMHOG000230814.
HOVERGENHBG014286.
InParanoidQ9P2G9.
KOK10446.
OMAADRYACE.
OrthoDBEOG7PK8Z1.
PhylomeDBQ9P2G9.
TreeFamTF329218.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9P2G9.
BgeeQ9P2G9.
CleanExHS_KLHL8.
GenevestigatorQ9P2G9.

Family and domain databases

Gene3D2.130.10.80. 1 hit.
3.30.710.10. 1 hit.
InterProIPR011705. BACK.
IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR015916. Gal_Oxidase_b-propeller.
IPR017096. Kelch-like_gigaxonin-typ.
IPR006652. Kelch_1.
[Graphical view]
PfamPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMSSF50965. SSF50965. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKLHL8.
GenomeRNAi57563.
NextBio64070.
PROQ9P2G9.
SOURCESearch...

Entry information

Entry nameKLHL8_HUMAN
AccessionPrimary (citable) accession number: Q9P2G9
Secondary accession number(s): Q53XA3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 104 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM