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Protein

E3 ubiquitin-protein ligase MARCH4

Gene

MARCH4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri155 – 215RING-CH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH4 (EC:2.3.2.27)
Alternative name(s):
Membrane-associated RING finger protein 4
Membrane-associated RING-CH protein IV
Short name:
MARCH-IV
RING finger protein 174
RING-type E3 ubiquitin transferase MARCH4Curated
Gene namesi
Name:MARCH4
Synonyms:KIAA1399, RNF174
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:29269. MARCH4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • Golgi stack Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • trans-Golgi network Source: UniProtKB

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57574.
OpenTargetsiENSG00000144583.
PharmGKBiPA134861849.

Polymorphism and mutation databases

BioMutaiMARCH4.
DMDMi59798475.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000005593019 – 410E3 ubiquitin-protein ligase MARCH4Add BLAST392

Proteomic databases

PaxDbiQ9P2E8.
PRIDEiQ9P2E8.

PTM databases

iPTMnetiQ9P2E8.
PhosphoSitePlusiQ9P2E8.

Expressioni

Tissue specificityi

Expressed in brain and placenta.1 Publication

Gene expression databases

BgeeiENSG00000144583.
CleanExiHS_MARCH4.
GenevisibleiQ9P2E8. HS.

Organism-specific databases

HPAiHPA014348.

Interactioni

Protein-protein interaction databases

BioGridi121627. 3 interactors.
STRINGi9606.ENSP00000273067.

Structurei

3D structure databases

ProteinModelPortaliQ9P2E8.
SMRiQ9P2E8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi57 – 127Pro-richAdd BLAST71

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri155 – 215RING-CH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110738.
HOGENOMiHOG000113484.
HOVERGENiHBG052412.
InParanoidiQ9P2E8.
KOiK10659.
OMAiMLMPLSG.
OrthoDBiEOG091G0JT5.
PhylomeDBiQ9P2E8.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiView protein in Pfam
PF12906. RINGv. 1 hit.
SMARTiView protein in SMART
SM00744. RINGv. 1 hit.
PROSITEiView protein in PROSITE
PS51292. ZF_RING_CH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P2E8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMPLCGLLW WWWCCCSGWY CYGLCAPAPQ MLRHQGLLKC RCRMLFNDLK
60 70 80 90 100
VFLLRRPPQA PLPMHGDPQP PGLAANNTLP ALGAGGWAGW RGPREVVGRE
110 120 130 140 150
PPPVPPPPPL PPSSVEDDWG GPATEPPASL LSSASSDDFC KEKTEDRYSL
160 170 180 190 200
GSSLDSGMRT PLCRICFQGP EQGELLSPCR CDGSVKCTHQ PCLIKWISER
210 220 230 240 250
GCWSCELCYY KYHVIAISTK NPLQWQAISL TVIEKVQVAA AILGSLFLIA
260 270 280 290 300
SISWLIWSTF SPSARWQRQD LLFQICYGMY GFMDVVCIGL IIHEGPSVYR
310 320 330 340 350
IFKRWQAVNQ QWKVLNYDKT KDLEDQKAGG RTNPRTSSST QANIPSSEEE
360 370 380 390 400
TAGTPAPEQG PAQAAGHPSG PLSHHHCAYT ILHILSHLRP HEQRSPPGSS
410
RELVMRVTTV
Length:410
Mass (Da):45,528
Last modified:February 15, 2005 - v2
Checksum:iBC812496FCCC2930
GO

Sequence cautioni

The sequence BAA92637 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037820 mRNA. Translation: BAA92637.1. Different initiation.
BC048793 mRNA. Translation: AAH48793.1.
BC098448 mRNA. Translation: AAH98448.1.
CCDSiCCDS33376.1.
RefSeqiNP_065865.1. NM_020814.2.
UniGeneiHs.170388.

Genome annotation databases

EnsembliENST00000273067; ENSP00000273067; ENSG00000144583.
GeneIDi57574.
KEGGihsa:57574.
UCSCiuc002vgb.4. human.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMARH4_HUMAN
AccessioniPrimary (citable) accession number: Q9P2E8
Secondary accession number(s): Q4KMN7, Q86WR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: June 7, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways