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Protein

Chondroitin sulfate glucuronyltransferase

Gene

CHPF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transfers glucuronic acid (GlcUA) from UDP-GlcUA to N-acetylgalactosamine residues on the non-reducing end of the elongating chondroitin polymer. Has no N-acetylgalactosaminyltransferase activity.2 Publications

Catalytic activityi

UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS12080-MONOMER.
BRENDAi2.4.1.226. 2681.
ReactomeiR-HSA-2022870. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate glucuronyltransferase (EC:2.4.1.226)
Alternative name(s):
CSGlcA-T
Chondroitin glucuronyltransferase
Chondroitin polymerizing factor 2
Short name:
ChPF-2
Chondroitin synthase 3
Short name:
ChSy-3
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase
Gene namesi
Name:CHPF2
Synonyms:CHSY3, CSGLCAT, KIAA1402
ORF Names:UNQ299/PRO339
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:29270. CHPF2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini30 – 772743LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi cisterna membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165617920.

Polymorphism and mutation databases

DMDMi67462204.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 772772Chondroitin sulfate glucuronyltransferasePRO_0000189563Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi121 – 1211N-linked (GlcNAc...)Sequence analysis
Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9P2E5.
MaxQBiQ9P2E5.
PaxDbiQ9P2E5.
PeptideAtlasiQ9P2E5.
PRIDEiQ9P2E5.

PTM databases

iPTMnetiQ9P2E5.
PhosphoSiteiQ9P2E5.

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in placenta, small intestine and pancreas.1 Publication

Gene expression databases

BgeeiQ9P2E5.
ExpressionAtlasiQ9P2E5. baseline and differential.
GenevisibleiQ9P2E5. HS.

Organism-specific databases

HPAiHPA020992.
HPA051582.

Interactioni

Protein-protein interaction databases

BioGridi119984. 6 interactions.
STRINGi9606.ENSP00000035307.

Structurei

3D structure databases

ProteinModelPortaliQ9P2E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi498 – 5058Poly-Ala
Compositional biasi628 – 65932Gly/Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3708. Eukaryota.
ENOG410XWZJ. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000037934.
InParanoidiQ9P2E5.
KOiK03419.
OMAiHLFRAVE.
OrthoDBiEOG790G17.
PhylomeDBiQ9P2E5.
TreeFamiTF318303.

Family and domain databases

InterProiIPR008428. Chond_GalNAc.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P2E5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLSSLLALL RPALPLILGL SLGCSLSLLR VSWIQGEGED PCVEAVGERG
60 70 80 90 100
GPQNPDSRAR LDQSDEDFKP RIVPYYRDPN KPYKKVLRTR YIQTELGSRE
110 120 130 140 150
RLLVAVLTSR ATLSTLAVAV NRTVAHHFPR LLYFTGQRGA RAPAGMQVVS
160 170 180 190 200
HGDERPAWLM SETLRHLHTH FGADYDWFFI MQDDTYVQAP RLAALAGHLS
210 220 230 240 250
INQDLYLGRA EEFIGAGEQA RYCHGGFGYL LSRSLLLRLR PHLDGCRGDI
260 270 280 290 300
LSARPDEWLG RCLIDSLGVG CVSQHQGQQY RSFELAKNRD PEKEGSSAFL
310 320 330 340 350
SAFAVHPVSE GTLMYRLHKR FSALELERAY SEIEQLQAQI RNLTVLTPEG
360 370 380 390 400
EAGLSWPVGL PAPFTPHSRF EVLGWDYFTE QHTFSCADGA PKCPLQGASR
410 420 430 440 450
ADVGDALETA LEQLNRRYQP RLRFQKQRLL NGYRRFDPAR GMEYTLDLLL
460 470 480 490 500
ECVTQRGHRR ALARRVSLLR PLSRVEILPM PYVTEATRVQ LVLPLLVAEA
510 520 530 540 550
AAAPAFLEAF AANVLEPREH ALLTLLLVYG PREGGRGAPD PFLGVKAAAA
560 570 580 590 600
ELERRYPGTR LAWLAVRAEA PSQVRLMDVV SKKHPVDTLF FLTTVWTRPG
610 620 630 640 650
PEVLNRCRMN AISGWQAFFP VHFQEFNPAL SPQRSPPGPP GAGPDPPSPP
660 670 680 690 700
GADPSRGAPI GGRFDRQASA EGCFYNADYL AARARLAGEL AGQEEEEALE
710 720 730 740 750
GLEVMDVFLR FSGLHLFRAV EPGLVQKFSL RDCSPRLSEE LYHRCRLSNL
760 770
EGLGGRAQLA MALFEQEQAN ST
Length:772
Mass (Da):85,948
Last modified:February 1, 2005 - v2
Checksum:iA4F6EC591919F4A2
GO
Isoform 2 (identifier: Q9P2E5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     638-666: GPPGAGPDPPSPPGADPSRGAPIGGRFDR → NLITFPLSASAPGRARQDGGQIENCCCIF
     667-772: Missing.

Note: No experimental confirmation available.
Show »
Length:666
Mass (Da):74,527
Checksum:iC450E883C6287B9B
GO

Sequence cautioni

The sequence BAA92640.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei638 – 66629GPPGA…GRFDR → NLITFPLSASAPGRARQDGG QIENCCCIF in isoform 2. 1 PublicationVSP_012724Add
BLAST
Alternative sequencei667 – 772106Missing in isoform 2. 1 PublicationVSP_012725Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095812 mRNA. Translation: BAG30817.1.
AB037823 mRNA. Translation: BAA92640.1. Different initiation.
AY358407 mRNA. Translation: AAQ88773.1.
CH471173 Genomic DNA. Translation: EAW54011.1.
BC064509 mRNA. Translation: AAH64509.1.
CCDSiCCDS34779.1. [Q9P2E5-1]
RefSeqiNP_061888.1. NM_019015.2. [Q9P2E5-1]
UniGeneiHs.647084.

Genome annotation databases

EnsembliENST00000035307; ENSP00000035307; ENSG00000033100. [Q9P2E5-1]
GeneIDi54480.
KEGGihsa:54480.
UCSCiuc003wjr.3. human. [Q9P2E5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB095812 mRNA. Translation: BAG30817.1.
AB037823 mRNA. Translation: BAA92640.1. Different initiation.
AY358407 mRNA. Translation: AAQ88773.1.
CH471173 Genomic DNA. Translation: EAW54011.1.
BC064509 mRNA. Translation: AAH64509.1.
CCDSiCCDS34779.1. [Q9P2E5-1]
RefSeqiNP_061888.1. NM_019015.2. [Q9P2E5-1]
UniGeneiHs.647084.

3D structure databases

ProteinModelPortaliQ9P2E5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119984. 6 interactions.
STRINGi9606.ENSP00000035307.

PTM databases

iPTMnetiQ9P2E5.
PhosphoSiteiQ9P2E5.

Polymorphism and mutation databases

DMDMi67462204.

Proteomic databases

EPDiQ9P2E5.
MaxQBiQ9P2E5.
PaxDbiQ9P2E5.
PeptideAtlasiQ9P2E5.
PRIDEiQ9P2E5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000035307; ENSP00000035307; ENSG00000033100. [Q9P2E5-1]
GeneIDi54480.
KEGGihsa:54480.
UCSCiuc003wjr.3. human. [Q9P2E5-1]

Organism-specific databases

CTDi54480.
GeneCardsiCHPF2.
H-InvDBHIX0007230.
HGNCiHGNC:29270. CHPF2.
HPAiHPA020992.
HPA051582.
MIMi608037. gene.
neXtProtiNX_Q9P2E5.
PharmGKBiPA165617920.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3708. Eukaryota.
ENOG410XWZJ. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000037934.
InParanoidiQ9P2E5.
KOiK03419.
OMAiHLFRAVE.
OrthoDBiEOG790G17.
PhylomeDBiQ9P2E5.
TreeFamiTF318303.

Enzyme and pathway databases

BioCyciMetaCyc:HS12080-MONOMER.
BRENDAi2.4.1.226. 2681.
ReactomeiR-HSA-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiCHPF2. human.
GenomeRNAii54480.
PROiQ9P2E5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9P2E5.
ExpressionAtlasiQ9P2E5. baseline and differential.
GenevisibleiQ9P2E5. HS.

Family and domain databases

InterProiIPR008428. Chond_GalNAc.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members."
    Izumikawa T., Koike T., Shiozawa S., Sugahara K., Tamura J., Kitagawa H.
    J. Biol. Chem. 283:11396-11406(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:65-73(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.
  6. "Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine."
    Gotoh M., Yada T., Sato T., Akashima T., Iwasaki H., Mochizuki H., Inaba N., Togayachi A., Kudo T., Watanabe H., Kimata K., Narimatsu H.
    J. Biol. Chem. 277:38179-38188(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHPF2_HUMAN
AccessioniPrimary (citable) accession number: Q9P2E5
Secondary accession number(s): B2DBD8, Q6P2I4, Q6UXD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: July 6, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.