Q9P2E5 (CHPF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chondroitin sulfate glucuronyltransferase
EC=2.4.1.226

Alternative name(s):
CSGlcA-T
Chondroitin glucuronyltransferase
Chondroitin polymerizing factor 2
Short name=ChPF-2
Chondroitin synthase 3
Short name=ChSy-3
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase

Gene names

Name:	CHPF2
Synonyms:	CHSY3, CSGLCAT, KIAA1402
ORF Names:	UNQ299/PRO339

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	772 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfers glucuronic acid (GlcUA) from UDP-GlcUA to N-acetylgalactosamine residues on the non-reducing end of the elongating chondroitin polymer. Has no N-acetylgalactosaminyltransferase activity. Ref.1 Ref.6
Catalytic activity	UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Ubiquitous. Highly expressed in placenta, small intestine and pancreas. Ref.6
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.
Sequence caution	The sequence BAA92640.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P2E5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P2E5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     638-666: GPPGAGPDPPSPPGADPSRGAPIGGRFDR → NLITFPLSASAPGRARQDGGQIENCCCIF
     667-772: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 772	772	Chondroitin sulfate glucuronyltransferase		PRO_0000189563
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 772	743	Lumenal Potential
	Compositional bias	498 – 505	8	Poly-Ala
	Compositional bias	628 – 659	32	Gly/Pro-rich
Amino acid modifications
	Glycosylation	121	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	638 – 666	29	GPPGA…GRFDR → NLITFPLSASAPGRARQDGG QIENCCCIF in isoform 2.		VSP_012724
	Alternative sequence	667 – 772	106	Missing in isoform 2.		VSP_012725

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: A4F6EC591919F4A2	FASTA	772	85,948
	10 20 30 40 50 60 MRLSSLLALL RPALPLILGL SLGCSLSLLR VSWIQGEGED PCVEAVGERG GPQNPDSRAR 70 80 90 100 110 120 LDQSDEDFKP RIVPYYRDPN KPYKKVLRTR YIQTELGSRE RLLVAVLTSR ATLSTLAVAV 130 140 150 160 170 180 NRTVAHHFPR LLYFTGQRGA RAPAGMQVVS HGDERPAWLM SETLRHLHTH FGADYDWFFI 190 200 210 220 230 240 MQDDTYVQAP RLAALAGHLS INQDLYLGRA EEFIGAGEQA RYCHGGFGYL LSRSLLLRLR 250 260 270 280 290 300 PHLDGCRGDI LSARPDEWLG RCLIDSLGVG CVSQHQGQQY RSFELAKNRD PEKEGSSAFL 310 320 330 340 350 360 SAFAVHPVSE GTLMYRLHKR FSALELERAY SEIEQLQAQI RNLTVLTPEG EAGLSWPVGL 370 380 390 400 410 420 PAPFTPHSRF EVLGWDYFTE QHTFSCADGA PKCPLQGASR ADVGDALETA LEQLNRRYQP 430 440 450 460 470 480 RLRFQKQRLL NGYRRFDPAR GMEYTLDLLL ECVTQRGHRR ALARRVSLLR PLSRVEILPM 490 500 510 520 530 540 PYVTEATRVQ LVLPLLVAEA AAAPAFLEAF AANVLEPREH ALLTLLLVYG PREGGRGAPD 550 560 570 580 590 600 PFLGVKAAAA ELERRYPGTR LAWLAVRAEA PSQVRLMDVV SKKHPVDTLF FLTTVWTRPG 610 620 630 640 650 660 PEVLNRCRMN AISGWQAFFP VHFQEFNPAL SPQRSPPGPP GAGPDPPSPP GADPSRGAPI 670 680 690 700 710 720 GGRFDRQASA EGCFYNADYL AARARLAGEL AGQEEEEALE GLEVMDVFLR FSGLHLFRAV 730 740 750 760 770 EPGLVQKFSL RDCSPRLSEE LYHRCRLSNL EGLGGRAQLA MALFEQEQAN ST
	Isoform 2 [UniParc]. Checksum: C450E883C6287B9B	FASTA	666	74,527
	10 20 30 40 50 60 MRLSSLLALL RPALPLILGL SLGCSLSLLR VSWIQGEGED PCVEAVGERG GPQNPDSRAR 70 80 90 100 110 120 LDQSDEDFKP RIVPYYRDPN KPYKKVLRTR YIQTELGSRE RLLVAVLTSR ATLSTLAVAV 130 140 150 160 170 180 NRTVAHHFPR LLYFTGQRGA RAPAGMQVVS HGDERPAWLM SETLRHLHTH FGADYDWFFI 190 200 210 220 230 240 MQDDTYVQAP RLAALAGHLS INQDLYLGRA EEFIGAGEQA RYCHGGFGYL LSRSLLLRLR 250 260 270 280 290 300 PHLDGCRGDI LSARPDEWLG RCLIDSLGVG CVSQHQGQQY RSFELAKNRD PEKEGSSAFL 310 320 330 340 350 360 SAFAVHPVSE GTLMYRLHKR FSALELERAY SEIEQLQAQI RNLTVLTPEG EAGLSWPVGL 370 380 390 400 410 420 PAPFTPHSRF EVLGWDYFTE QHTFSCADGA PKCPLQGASR ADVGDALETA LEQLNRRYQP 430 440 450 460 470 480 RLRFQKQRLL NGYRRFDPAR GMEYTLDLLL ECVTQRGHRR ALARRVSLLR PLSRVEILPM 490 500 510 520 530 540 PYVTEATRVQ LVLPLLVAEA AAAPAFLEAF AANVLEPREH ALLTLLLVYG PREGGRGAPD 550 560 570 580 590 600 PFLGVKAAAA ELERRYPGTR LAWLAVRAEA PSQVRLMDVV SKKHPVDTLF FLTTVWTRPG 610 620 630 640 650 660 PEVLNRCRMN AISGWQAFFP VHFQEFNPAL SPQRSPPNLI TFPLSASAPG RARQDGGQIE NCCCIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members." Izumikawa T., Koike T., Shiozawa S., Sugahara K., Tamura J., Kitagawa H. J. Biol. Chem. 283:11396-11406(2008) [PubMed: 18316376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[6]	"Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine." Gotoh M., Yada T., Sato T., Akashima T., Iwasaki H., Mochizuki H., Inaba N., Togayachi A., Kudo T., Watanabe H., Kimata K., Narimatsu H. J. Biol. Chem. 277:38179-38188(2002) [PubMed: 12145278] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB095812 mRNA. Translation: BAG30817.1. AB037823 mRNA. Translation: BAA92640.1. Different initiation. AY358407 mRNA. Translation: AAQ88773.1. CH471173 Genomic DNA. Translation: EAW54011.1. BC064509 mRNA. Translation: AAH64509.1.
IPI	IPI00018606. IPI00551043.
RefSeq	NP_061888.1. NM_019015.1.
UniGene	Hs.647084.
3D structure databases
ProteinModelPortal	Q9P2E5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9P2E5.
PTM databases
PhosphoSite	Q9P2E5.
Polymorphism databases
DMDM	67462204.
Proteomic databases
PRIDE	Q9P2E5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000035307; ENSP00000035307; ENSG00000033100. ENST00000463723; ENSP00000417859; ENSG00000033100.
GeneID	54480.
KEGG	hsa:54480.
UCSC	uc003wjr.1. human.
Organism-specific databases
CTD	54480.
GeneCards	GC07P150929.
H-InvDB	HIX0007230.
HGNC	HGNC:29270. CHPF2.
HPA	HPA020992.
MIM	608037. gene.
neXtProt	NX_Q9P2E5.
PharmGKB	PA165617920.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09982.
GeneTree	ENSGT00530000062787.
HOGENOM	HBG402989.
InParanoid	Q9P2E5.
OMA	HLFRAVE.
PhylomeDB	Q9P2E5.
Enzyme and pathway databases
BioCyc	MetaCyc:HS12080-MONOMER.
BRENDA	2.4.1.226. 2681.
Gene expression databases
ArrayExpress	Q9P2E5.
Bgee	Q9P2E5.
Genevestigator	Q9P2E5.
GermOnline	ENSG00000033100. Homo sapiens.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. [Graphical view]
KO	K03419.
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	56800.
SOURCE	Search...

Entry information

Entry name

CHPF2_HUMAN

Accession

Primary (citable) accession number: Q9P2E5
Secondary accession number(s): B2DBD8, Q6P2I4, Q6UXD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.