Q9P2A4 (ABI3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ABI gene family member 3

Alternative name(s):
New molecule including SH3
Short name=Nesh

Gene names

Name:	ABI3
Synonyms:	NESH

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May inhibit tumor metastasis By similarity. In vitro, reduces cell motility. Ref.5
Subunit structure	May interact with PAK1 and PAK2. Probably interacts with TARSH. Ref.6
Subcellular location	Cytoplasm. Note: Colocalizes with PAK2 at leading edge of cells. Ref.5
Tissue specificity	Expressed in heart, lung, liver, pancreas, kidney, placenta and at low levels in brain and skeletal muscle. Ref.1
Sequence similarities	Belongs to the ABI family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Non-traceable author statement Ref.5. Source: UniProtKB regulation of cell migration Inferred from direct assay Ref.5. Source: MGI
Cellular_component	cytoplasm Non-traceable author statement Ref.5. Source: UniProtKB lamellipodium Inferred from direct assay PubMed 17101133. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF3H	O15372	3	EBI-742038,EBI-709735

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P2A4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P2A4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-95: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	ABI gene family member 3		PRO_0000191792
Regions
	Domain	308 – 366	59	SH3
	Coiled coil	33 – 61	29	Potential
	Compositional bias	224 – 306	83	Pro-rich
Natural variations
	Alternative sequence	90 – 95	6	Missing in isoform 2.		VSP_041467
	Natural variant	44	1	R → Q. Ref.2 Corresponds to variant rs2233369 [ dbSNP | Ensembl ].		VAR_022030
	Natural variant	203	1	S → F. Corresponds to variant rs616338 [ dbSNP | Ensembl ].		VAR_060243
	Natural variant	209	1	F → S. Ref.1 Ref.2 Ref.4 Corresponds to variant rs616338 [ dbSNP | Ensembl ].		VAR_060993

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 23EC3838E41D8ECD	FASTA	366	39,035
	10 20 30 40 50 60 MAELQQLQEF EIPTGREALR GNHSALLRVA DYCEDNYVQA TDKRKALEET MAFTTQALAS 70 80 90 100 110 120 VAYQVGNLAG HTLRMLDLQG AALRQVEARV STLGQMVNMH MEKVARREIG TLATVQRLPP 130 140 150 160 170 180 GQKVIAPENL PPLTPYCRRP LNFGCLDDIG HGIKDLSTQL SRTGTLSRKS IKAPATPASA 190 200 210 220 230 240 TLGRPPRIPE PVHLPVVPDG RLSAASSAFS LASAGSAEGV GGAPTPKGQA APPAPPLPSS 250 260 270 280 290 300 LDPPPPPAAV EVFQRPPTLE ELSPPPPDEE LPLPLDLPPP PPLDGDELGL PPPPPGFGPD 310 320 330 340 350 360 EPSWVPASYL EKVVTLYPYT SQKDNELSFS EGTVICVTRR YSDGWCEGVS SEGTGFFPGN YVEPSC
	Isoform 2 [UniParc]. Checksum: 1DB04BD6FFA0ECF1	FASTA	360	38,450
	10 20 30 40 50 60 MAELQQLQEF EIPTGREALR GNHSALLRVA DYCEDNYVQA TDKRKALEET MAFTTQALAS 70 80 90 100 110 120 VAYQVGNLAG HTLRMLDLQG AALRQVEARM VNMHMEKVAR REIGTLATVQ RLPPGQKVIA 130 140 150 160 170 180 PENLPPLTPY CRRPLNFGCL DDIGHGIKDL STQLSRTGTL SRKSIKAPAT PASATLGRPP 190 200 210 220 230 240 RIPEPVHLPV VPDGRLSAAS SAFSLASAGS AEGVGGAPTP KGQAAPPAPP LPSSLDPPPP 250 260 270 280 290 300 PAAVEVFQRP PTLEELSPPP PDEELPLPLD LPPPPPLDGD ELGLPPPPPG FGPDEPSWVP 310 320 330 340 350 360 ASYLEKVVTL YPYTSQKDNE LSFSEGTVIC VTRRYSDGWC EGVSSEGTGF FPGNYVEPSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a novel human gene (NESH) which encodes a putative signaling molecule similar to e3B1 protein." Miyazaki K., Matsuda S., Ichigotani Y., Takenouchi Y., Hayashi K., Fukuda Y., Nimura Y., Hamaguchi M. Biochim. Biophys. Acta 1493:237-241(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANT SER-209. Tissue: Placenta.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS GLN-44 AND SER-209. Tissue: Kidney.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-209. Tissue: B-cell.
[5]	"Forced expression of NESH suppresses motility and metastatic dissemination of malignant cells." Ichigotani Y., Yokozaki S., Fukuda Y., Hamaguchi M., Matsuda S. Cancer Res. 62:2215-2219(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
[6]	"Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3." Matsuda S., Iriyama C., Yokozaki S., Ichigotani Y., Shirafuji N., Yamaki K., Hayakawa T., Hamaguchi M. J. Hum. Genet. 46:483-486(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TARSH.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB037886 mRNA. Translation: BAA90667.1. AL136709 mRNA. Translation: CAB66644.1. AC004797 Genomic DNA. No translation available. AC069454 Genomic DNA. No translation available. BC007780 mRNA. Translation: AAH07780.1.
IPI	IPI00009273. IPI00914068.
RefSeq	NP_001128658.1. NM_001135186.1. NP_057512.1. NM_016428.2.
UniGene	Hs.130719.
3D structure databases
ProteinModelPortal	Q9P2A4.
SMR	Q9P2A4. Positions 1-154, 301-363.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P2A4. 12 interactions.
MINT	MINT-235637.
STRING	9606.ENSP00000225941.
PTM databases
PhosphoSite	Q9P2A4.
Polymorphism databases
DMDM	296434384.
Proteomic databases
PaxDb	Q9P2A4.
PRIDE	Q9P2A4.
Protocols and materials databases
DNASU	51225.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000225941; ENSP00000225941; ENSG00000108798. ENST00000419580; ENSP00000406651; ENSG00000108798.
GeneID	51225.
KEGG	hsa:51225.
UCSC	uc002iop.1. human. uc002ioq.1. human.
Organism-specific databases
CTD	51225.
GeneCards	GC17P047287.
HGNC	HGNC:29859. ABI3.
HPA	HPA017345.
MIM	606363. gene.
neXtProt	NX_Q9P2A4.
PharmGKB	PA134951642.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG328966.
HOGENOM	HOG000293213.
HOVERGEN	HBG050446.
InParanoid	Q9P2A4.
OMA	HGIKDLS.
OrthoDB	EOG4CRM0Q.
PhylomeDB	Q9P2A4.
Gene expression databases
Bgee	Q9P2A4.
CleanEx	HS_ABI3.
Genevestigator	Q9P2A4.
GermOnline	ENSG00000108798. Homo sapiens.
Family and domain databases
InterPro	IPR012849. Abl-interactor_HHR_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF07815. Abi_HHR. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51225.
NextBio	54310.
SOURCE	Search...

Entry information

Entry name

ABI3_HUMAN

Accession

Primary (citable) accession number: Q9P2A4
Secondary accession number(s): C9IZN8, Q9H0P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.