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Q9P296 (C5AR2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
C5a anaphylatoxin chemotactic receptor 2
Alternative name(s):
Complement component 5a receptor 2
G-protein coupled receptor 77
Gene names
Name:C5AR2
Synonyms:C5L2, GPR77
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Ref.8 Ref.10 Ref.11

Subunit structure

Interacts with C3 (the anaphylatoxin peptide C3a and the adipogenic hormone ASP); the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of GPR77, recruitment of ARRB2 to the cell surface and endocytosis of GRP77. Ref.9

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.11.

Tissue specificity

Frontal cortex, hippocampus, hypothalamus, pons and liver. Ref.1 Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337C5a anaphylatoxin chemotactic receptor 2
PRO_0000069216

Regions

Topological domain1 – 3838Extracellular Potential
Transmembrane39 – 6123Helical; Name=1; Potential
Topological domain62 – 7211Cytoplasmic Potential
Transmembrane73 – 9523Helical; Name=2; Potential
Topological domain96 – 11419Extracellular Potential
Transmembrane115 – 13723Helical; Name=3; Potential
Topological domain138 – 14912Cytoplasmic Potential
Transmembrane150 – 17223Helical; Name=4; Potential
Topological domain173 – 20230Extracellular Potential
Transmembrane203 – 22523Helical; Name=5; Potential
Topological domain226 – 23712Cytoplasmic Potential
Transmembrane238 – 26023Helical; Name=6; Potential
Topological domain261 – 27414Extracellular Potential
Transmembrane275 – 29420Helical; Name=7; Potential
Topological domain295 – 33743Cytoplasmic Potential

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Disulfide bond107 ↔ 186 By similarity

Natural variations

Natural variant2331P → L. Ref.13
Corresponds to variant rs149572881 [ dbSNP | Ensembl ].
VAR_068748
Natural variant3231S → I Rare polymorphism found in a family with familial combined hyperlipemia; associated with increased plasma triglyceride, plasma cholesterol, low-density lipoprotein cholesterol, apolipoprotein B and ASP. Ref.11 Ref.12
Corresponds to variant rs150599989 [ dbSNP | Ensembl ].
VAR_068749

Sequences

Sequence LengthMass (Da)Tools
Q9P296 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 53AF41B129FE8FE6

FASTA33736,080
        10         20         30         40         50         60 
MGNDSVSYEY GDYSDLSDRP VDCLDGACLA IDPLRVAPLP LYAAIFLVGV PGNAMVAWVA 

        70         80         90        100        110        120 
GKVARRRVGA TWLLHLAVAD LLCCLSLPIL AVPIARGGHW PYGAVGCRAL PSIILLTMYA 

       130        140        150        160        170        180 
SVLLLAALSA DLCFLALGPA WWSTVQRACG VQVACGAAWT LALLLTVPSA IYRRLHQEHF 

       190        200        210        220        230        240 
PARLQCVVDY GGSSSTENAV TAIRFLFGFL GPLVAVASCH SALLCWAARR CRPLGTAIVV 

       250        260        270        280        290        300 
GFFVCWAPYH LLGLVLTVAA PNSALLARAL RAEPLIVGLA LAHSCLNPML FLYFGRAQLR 

       310        320        330 
RSLPAACHWA LRESQGQDES VDSKKSTSHD LVSEMEV 

« Hide

References

« Hide 'large scale' references
[1]"A putative chemoattractant receptor, C5L2, is expressed in granulocyte and immature dendritic cells, but not in mature dendritic cells."
Ohno M., Hirata T., Enomoto M., Araki T., Ishimaru H., Takahashi T.A.
Mol. Immunol. 37:407-412(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Placenta.
[2]"Identification of four novel human G protein-coupled receptors expressed in the brain."
Lee D.K., George S.R., Cheng R., Nguyen T., Liu Y., Brown M., Lynch K.R., O'Dowd B.F.
Brain Res. Mol. Brain Res. 86:13-22(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Kopatz S.A., Aronstam R.S., Sharma S.V.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Trachea.
[5]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]"The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des Arg(74)."
Cain S.A., Monk P.N.
J. Biol. Chem. 277:7165-7169(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Tissue: Brain.
[9]"The chemoattractant receptor-like protein C5L2 binds the C3a des-Arg77/acylation-stimulating protein."
Kalant D., Cain S.A., Maslowska M., Sniderman A.D., Cianflone K., Monk P.N.
J. Biol. Chem. 278:11123-11129(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH C3 ADIOPOGENIC PEPTIDE ASP.
[10]"C5L2 is a functional receptor for acylation-stimulating protein."
Kalant D., MacLaren R., Cui W., Samanta R., Monk P.N., Laporte S.A., Cianflone K.
J. Biol. Chem. 280:23936-23944(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A RECEPTOR FOR THE C3 ADIOPOGENIC PEPTIDE ASP.
[11]"C5a- and ASP-mediated C5L2 activation, endocytosis and recycling are lost in S323I-C5L2 mutation."
Cui W., Simaan M., Laporte S., Lodge R., Cianflone K.
Mol. Immunol. 46:3086-3098(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, CHARACTERIZATION OF VARIANT ILE-323.
[12]"Identification of a novel C5L2 variant (S323I) in a French Canadian family with familial combined hyperlipemia."
Marcil M., Vu H., Cui W., Dastani Z., Engert J.C., Gaudet D., Castro-Cabezas M., Sniderman A.D., Genest J. Jr., Cianflone K.
Arterioscler. Thromb. Vasc. Biol. 26:1619-1625(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT ILE-323.
[13]"Relationship between a novel polymorphism of the C5L2 gene and coronary artery disease."
Zheng Y.Y., Xie X., Ma Y.T., Yang Y.N., Fu Z.Y., Li X.M., Ma X., Chen B.D., Liu F.
PLoS ONE 6:E20984-E20984(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT LEU-233.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038237 mRNA. Translation: BAA95414.1.
AF317655 Genomic DNA. Translation: AAK12640.1.
AY268430 Genomic DNA. Translation: AAP23197.1.
AK313994 mRNA. Translation: BAG36706.1.
AC008754 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW57472.1.
BC067478 mRNA. Translation: AAH67478.1.
RefSeqNP_001258678.1. NM_001271749.1.
NP_001258679.1. NM_001271750.1.
NP_060955.1. NM_018485.2.
UniGeneHs.534412.

3D structure databases

ProteinModelPortalQ9P296.
SMRQ9P296. Positions 34-294.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9P296. 1 interaction.
STRING9606.ENSP00000257267.

Chemistry

GuidetoPHARMACOLOGY33.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9P296.

Polymorphism databases

DMDM20137533.

Proteomic databases

PRIDEQ9P296.

Protocols and materials databases

DNASU27202.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000257267; ENSP00000257267; ENSG00000134830.
ENST00000595464; ENSP00000472620; ENSG00000134830.
ENST00000600626; ENSP00000471184; ENSG00000134830.
GeneID27202.
KEGGhsa:27202.
UCSCuc002pgk.2. human.

Organism-specific databases

CTD27202.
GeneCardsGC19P047841.
HGNCHGNC:4527. C5AR2.
HPAHPA016629.
MIM609949. gene.
neXtProtNX_Q9P296.
PharmGKBPA28920.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG151055.
HOGENOMHOG000234122.
HOVERGENHBG107547.
InParanoidQ9P296.
KOK04171.
OMAAACHWAL.
OrthoDBEOG7G1V6M.
PhylomeDBQ9P296.
TreeFamTF330976.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeQ9P296.
CleanExHS_GPR77.
GenevestigatorQ9P296.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001274. Anaphtx_C5AR1/C5AR2.
IPR027810. Anaphtx_C5AR2.
IPR000826. Formyl_pep_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24225. PTHR24225. 1 hit.
PTHR24225:SF1. PTHR24225:SF1. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00426. C5ANPHYLTXNR.
PR00237. GPCRRHODOPSN.
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR77.
GenomeRNAi27202.
NextBio50076.
PROQ9P296.
SOURCESearch...

Entry information

Entry nameC5AR2_HUMAN
AccessionPrimary (citable) accession number: Q9P296
Secondary accession number(s): B2RA09
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries