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Q9P296

- C5AR2_HUMAN

UniProt

Q9P296 - C5AR2_HUMAN

Protein

C5a anaphylatoxin chemotactic receptor 2

Gene

C5AR2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways.3 Publications

    GO - Molecular functioni

    1. C5a anaphylatoxin receptor activity Source: InterPro
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. chemotaxis Source: InterPro
    2. inflammatory response Source: InterPro
    3. negative regulation of interleukin-6 secretion Source: UniProtKB
    4. negative regulation of neutrophil chemotaxis Source: UniProtKB
    5. negative regulation of tumor necrosis factor production Source: UniProtKB
    6. positive regulation of epithelial cell proliferation Source: InterPro
    7. positive regulation of ERK1 and ERK2 cascade Source: InterPro
    8. regulation of interleukin-8 secretion Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    C5a anaphylatoxin chemotactic receptor 2
    Alternative name(s):
    Complement component 5a receptor 2
    G-protein coupled receptor 77
    Gene namesi
    Name:C5AR2
    Synonyms:C5L2, GPR77
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:4527. C5AR2.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. apical part of cell Source: UniProtKB
    2. basal plasma membrane Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28920.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337C5a anaphylatoxin chemotactic receptor 2PRO_0000069216Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi107 ↔ 186PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9P296.

    PTM databases

    PhosphoSiteiQ9P296.

    Expressioni

    Tissue specificityi

    Frontal cortex, hippocampus, hypothalamus, pons and liver.2 Publications

    Gene expression databases

    BgeeiQ9P296.
    CleanExiHS_GPR77.
    GenevestigatoriQ9P296.

    Organism-specific databases

    HPAiHPA016629.

    Interactioni

    Subunit structurei

    Interacts with C3 (the anaphylatoxin peptide C3a and the adipogenic hormone ASP); the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of GPR77, recruitment of ARRB2 to the cell surface and endocytosis of GRP77.1 Publication

    Protein-protein interaction databases

    IntActiQ9P296. 1 interaction.
    STRINGi9606.ENSP00000257267.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P296.
    SMRiQ9P296. Positions 34-294.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3838ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini62 – 7211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini96 – 11419ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini138 – 14912CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini173 – 20230ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini226 – 23712CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini261 – 27414ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini295 – 33743CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei39 – 6123Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei73 – 9523Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei115 – 13723Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei150 – 17223Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei203 – 22523Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei238 – 26023Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei275 – 29420Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG151055.
    HOGENOMiHOG000234122.
    HOVERGENiHBG107547.
    InParanoidiQ9P296.
    KOiK04171.
    OMAiAACHWAL.
    OrthoDBiEOG7G1V6M.
    PhylomeDBiQ9P296.
    TreeFamiTF330976.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001274. Anaphtx_C5AR1/C5AR2.
    IPR027809. Anaphtx_C5AR2.
    IPR000826. Formyl_rcpt-rel.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24225. PTHR24225. 1 hit.
    PTHR24225:SF1. PTHR24225:SF1. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00426. C5ANPHYLTXNR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9P296-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNDSVSYEY GDYSDLSDRP VDCLDGACLA IDPLRVAPLP LYAAIFLVGV    50
    PGNAMVAWVA GKVARRRVGA TWLLHLAVAD LLCCLSLPIL AVPIARGGHW 100
    PYGAVGCRAL PSIILLTMYA SVLLLAALSA DLCFLALGPA WWSTVQRACG 150
    VQVACGAAWT LALLLTVPSA IYRRLHQEHF PARLQCVVDY GGSSSTENAV 200
    TAIRFLFGFL GPLVAVASCH SALLCWAARR CRPLGTAIVV GFFVCWAPYH 250
    LLGLVLTVAA PNSALLARAL RAEPLIVGLA LAHSCLNPML FLYFGRAQLR 300
    RSLPAACHWA LRESQGQDES VDSKKSTSHD LVSEMEV 337
    Length:337
    Mass (Da):36,080
    Last modified:October 1, 2000 - v1
    Checksum:i53AF41B129FE8FE6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti233 – 2331P → L.1 Publication
    Corresponds to variant rs149572881 [ dbSNP | Ensembl ].
    VAR_068748
    Natural varianti323 – 3231S → I Rare polymorphism found in a family with familial combined hyperlipemia; associated with increased plasma triglyceride, plasma cholesterol, low-density lipoprotein cholesterol, apolipoprotein B and ASP. 1 Publication
    Corresponds to variant rs150599989 [ dbSNP | Ensembl ].
    VAR_068749

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038237 mRNA. Translation: BAA95414.1.
    AF317655 Genomic DNA. Translation: AAK12640.1.
    AY268430 Genomic DNA. Translation: AAP23197.1.
    AK313994 mRNA. Translation: BAG36706.1.
    AC008754 Genomic DNA. No translation available.
    CH471126 Genomic DNA. Translation: EAW57472.1.
    BC067478 mRNA. Translation: AAH67478.1.
    CCDSiCCDS12699.1.
    RefSeqiNP_001258678.1. NM_001271749.1.
    NP_001258679.1. NM_001271750.1.
    NP_060955.1. NM_018485.2.
    UniGeneiHs.534412.

    Genome annotation databases

    EnsembliENST00000595464; ENSP00000472620; ENSG00000134830.
    ENST00000600626; ENSP00000471184; ENSG00000134830.
    GeneIDi27202.
    KEGGihsa:27202.
    UCSCiuc002pgk.2. human.

    Polymorphism databases

    DMDMi20137533.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038237 mRNA. Translation: BAA95414.1 .
    AF317655 Genomic DNA. Translation: AAK12640.1 .
    AY268430 Genomic DNA. Translation: AAP23197.1 .
    AK313994 mRNA. Translation: BAG36706.1 .
    AC008754 Genomic DNA. No translation available.
    CH471126 Genomic DNA. Translation: EAW57472.1 .
    BC067478 mRNA. Translation: AAH67478.1 .
    CCDSi CCDS12699.1.
    RefSeqi NP_001258678.1. NM_001271749.1.
    NP_001258679.1. NM_001271750.1.
    NP_060955.1. NM_018485.2.
    UniGenei Hs.534412.

    3D structure databases

    ProteinModelPortali Q9P296.
    SMRi Q9P296. Positions 34-294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9P296. 1 interaction.
    STRINGi 9606.ENSP00000257267.

    Chemistry

    GuidetoPHARMACOLOGYi 33.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q9P296.

    Polymorphism databases

    DMDMi 20137533.

    Proteomic databases

    PRIDEi Q9P296.

    Protocols and materials databases

    DNASUi 27202.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000595464 ; ENSP00000472620 ; ENSG00000134830 .
    ENST00000600626 ; ENSP00000471184 ; ENSG00000134830 .
    GeneIDi 27202.
    KEGGi hsa:27202.
    UCSCi uc002pgk.2. human.

    Organism-specific databases

    CTDi 27202.
    GeneCardsi GC19P047841.
    HGNCi HGNC:4527. C5AR2.
    HPAi HPA016629.
    MIMi 609949. gene.
    neXtProti NX_Q9P296.
    PharmGKBi PA28920.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG151055.
    HOGENOMi HOG000234122.
    HOVERGENi HBG107547.
    InParanoidi Q9P296.
    KOi K04171.
    OMAi AACHWAL.
    OrthoDBi EOG7G1V6M.
    PhylomeDBi Q9P296.
    TreeFami TF330976.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.

    Miscellaneous databases

    GeneWikii GPR77.
    GenomeRNAii 27202.
    NextBioi 50076.
    PROi Q9P296.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9P296.
    CleanExi HS_GPR77.
    Genevestigatori Q9P296.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001274. Anaphtx_C5AR1/C5AR2.
    IPR027809. Anaphtx_C5AR2.
    IPR000826. Formyl_rcpt-rel.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24225. PTHR24225. 1 hit.
    PTHR24225:SF1. PTHR24225:SF1. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00426. C5ANPHYLTXNR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A putative chemoattractant receptor, C5L2, is expressed in granulocyte and immature dendritic cells, but not in mature dendritic cells."
      Ohno M., Hirata T., Enomoto M., Araki T., Ishimaru H., Takahashi T.A.
      Mol. Immunol. 37:407-412(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Placenta.
    2. "Identification of four novel human G protein-coupled receptors expressed in the brain."
      Lee D.K., George S.R., Cheng R., Nguyen T., Liu Y., Brown M., Lynch K.R., O'Dowd B.F.
      Brain Res. Mol. Brain Res. 86:13-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    3. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Kopatz S.A., Aronstam R.S., Sharma S.V.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Trachea.
    5. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    8. "The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des Arg(74)."
      Cain S.A., Monk P.N.
      J. Biol. Chem. 277:7165-7169(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Tissue: Brain.
    9. "The chemoattractant receptor-like protein C5L2 binds the C3a des-Arg77/acylation-stimulating protein."
      Kalant D., Cain S.A., Maslowska M., Sniderman A.D., Cianflone K., Monk P.N.
      J. Biol. Chem. 278:11123-11129(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH C3 ADIOPOGENIC PEPTIDE ASP.
    10. "C5L2 is a functional receptor for acylation-stimulating protein."
      Kalant D., MacLaren R., Cui W., Samanta R., Monk P.N., Laporte S.A., Cianflone K.
      J. Biol. Chem. 280:23936-23944(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A RECEPTOR FOR THE C3 ADIOPOGENIC PEPTIDE ASP.
    11. "C5a- and ASP-mediated C5L2 activation, endocytosis and recycling are lost in S323I-C5L2 mutation."
      Cui W., Simaan M., Laporte S., Lodge R., Cianflone K.
      Mol. Immunol. 46:3086-3098(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, CHARACTERIZATION OF VARIANT ILE-323.
    12. "Identification of a novel C5L2 variant (S323I) in a French Canadian family with familial combined hyperlipemia."
      Marcil M., Vu H., Cui W., Dastani Z., Engert J.C., Gaudet D., Castro-Cabezas M., Sniderman A.D., Genest J. Jr., Cianflone K.
      Arterioscler. Thromb. Vasc. Biol. 26:1619-1625(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT ILE-323.
    13. "Relationship between a novel polymorphism of the C5L2 gene and coronary artery disease."
      Zheng Y.Y., Xie X., Ma Y.T., Yang Y.N., Fu Z.Y., Li X.M., Ma X., Chen B.D., Liu F.
      PLoS ONE 6:E20984-E20984(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT LEU-233.

    Entry informationi

    Entry nameiC5AR2_HUMAN
    AccessioniPrimary (citable) accession number: Q9P296
    Secondary accession number(s): B2RA09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3