Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9P275 (UBP36_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 36

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene names
Name:USP36
Synonyms:KIAA1453
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1121 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.6

Subunit structure

Interacts with PAF1 By similarity.

Subcellular location

Nucleusnucleolus Ref.5.

Tissue specificity

Broadly expressed. Ref.6

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence AAH16487.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence BAA95977.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P275-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P275-2)

The sequence of this isoform differs from the canonical sequence as follows:
     958-958: K → KKK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11211121Ubiquitin carboxyl-terminal hydrolase 36
PRO_0000080666

Regions

Domain122 – 423302USP

Sites

Active site1311Nucleophile By similarity
Active site3821Proton acceptor By similarity

Amino acid modifications

Modified residue4641Phosphoserine Ref.8
Modified residue5821Phosphoserine Ref.8 Ref.11
Modified residue6671Phosphoserine Ref.8 Ref.10
Modified residue6821Phosphoserine Ref.10
Modified residue7131Phosphoserine Ref.11
Modified residue7421Phosphoserine Ref.11 Ref.12
Modified residue9521Phosphoserine Ref.7 Ref.11

Natural variations

Alternative sequence9581K → KKK in isoform 2.
VSP_024382
Natural variant2711V → I. Ref.2
Corresponds to variant rs3744793 [ dbSNP | Ensembl ].
VAR_037277
Natural variant4891I → M.
Corresponds to variant rs3744795 [ dbSNP | Ensembl ].
VAR_037278
Natural variant7751R → Q.
Corresponds to variant rs9889908 [ dbSNP | Ensembl ].
VAR_037279
Natural variant8061Q → R. Ref.1 Ref.2 Ref.3
Corresponds to variant rs3088040 [ dbSNP | Ensembl ].
VAR_037280
Natural variant8141K → N.
Corresponds to variant rs3744797 [ dbSNP | Ensembl ].
VAR_037281
Natural variant8281R → C. Ref.1 Ref.3
Corresponds to variant rs1057040 [ dbSNP | Ensembl ].
VAR_037282
Natural variant8871R → P. Ref.2
Corresponds to variant rs61760231 [ dbSNP | Ensembl ].
VAR_058034

Experimental info

Sequence conflict821R → G in AAH71582. Ref.2
Sequence conflict5731D → G in BAA91825. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 23E11A75B20304B7

FASTA1,121122,652
        10         20         30         40         50         60 
MPIVDKLKEA LKPGRKDSAD DGELGKLLAS SAKKVLLQKI EFEPASKSFS YQLEALKSKY 

        70         80         90        100        110        120 
VLLNPKTEGA SRHKSGDDPP ARRQGSEHTY ESCGDGVPAP QKVLFPTERL SLRWERVFRV 

       130        140        150        160        170        180 
GAGLHNLGNT CFLNATIQCL TYTPPLANYL LSKEHARSCH QGSFCMLCVM QNHIVQAFAN 

       190        200        210        220        230        240 
SGNAIKPVSF IRDLKKIARH FRFGNQEDAH EFLRYTIDAM QKACLNGCAK LDRQTQATTL 

       250        260        270        280        290        300 
VHQIFGGYLR SRVKCSVCKS VSDTYDPYLD VALEIRQAAN IVRALELFVK ADVLSGENAY 

       310        320        330        340        350        360 
MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN IRPYMSQNNG 

       370        380        390        400        410        420 
DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS LVHSSNVKVV LNQQAYVLFY 

       430        440        450        460        470        480 
LRIPGSKKSP EGLISRTGSS SLPGRPSVIP DHSKKNIGNG IISSPLTGKR QDSGTMKKPH 

       490        500        510        520        530        540 
TTEEIGVPIS RNGSTLGLKS QNGCIPPKLP SGSPSPKLSQ TPTHMPTILD DPGKKVKKPA 

       550        560        570        580        590        600 
PPQHFSPRTA QGLPGTSNSN SSRSGSQRQG SWDSRDVVLS TSPKLLATAT ANGHGLKGND 

       610        620        630        640        650        660 
ESAGLDRRGS SSSSPEHSAS SDSTKAPQTP RSGAAHLCDS QETNCSTAGH SKTPPSGADS 

       670        680        690        700        710        720 
KTVKLKSPVL SNTTTEPAST MSPPPAKKLA LSAKKASTLW RATGNDLRPP PPSPSSDLTH 

       730        740        750        760        770        780 
PMKTSHPVVA STWPVHRARA VSPAPQSSSR LQPPFSPHPT LLSSTPKPPG TSEPRSCSSI 

       790        800        810        820        830        840 
STALPQVNED LVSLPHQLPE ASEPPQSPSE KRKKTFVGEP QRLGSETRLP QHIREATAAP 

       850        860        870        880        890        900 
HGKRKRKKKK RPEDTAASAL QEGQTQRQPG SPMYRREGQA QLPAVRRQED GTQPQVNGQQ 

       910        920        930        940        950        960 
VGCVTDGHHA SSRKRRRKGA EGLGEEGGLH QDPLRHSCSP MGDGDPEAME ESPRKKKKRK 

       970        980        990       1000       1010       1020 
QETQRAVEED GHLKCPRSAK PQDAVVPESS SCAPSANGWC PGDRMGLSQA PPVSWNGERE 

      1030       1040       1050       1060       1070       1080 
SDVVQELLKY SSDKAYGRKV LTWDGKMSAV SQDAIEDSRQ ARTETVVDDW DEEFDRGKEK 

      1090       1100       1110       1120 
KIKKFKREKR RNFNAFQKLQ TRRNFWSVTH PAKAASLSYR R 

« Hide

Isoform 2 [UniParc].

Checksum: 65CD93C1B8655319
Show »

FASTA1,123122,908

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ARG-806 AND CYS-828.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ILE-271; ARG-806 AND PRO-887.
Tissue: Cervix, Placenta and Skin.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-954 (ISOFORM 1), VARIANTS ARG-806 AND CYS-828.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 494-1121 (ISOFORM 2).
Tissue: Testis.
[5]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, ENZYME ACTIVITY.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-952, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464; SER-582 AND SER-667, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-667 AND SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582; SER-713; SER-742 AND SER-952, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB040886 mRNA. Translation: BAA95977.1. Different initiation.
BC016487 mRNA. Translation: AAH16487.1. Sequence problems.
BC027992 mRNA. Translation: AAH27992.1.
BC071582 mRNA. Translation: AAH71582.1.
AK001671 mRNA. Translation: BAA91825.1.
AL833835 mRNA. Translation: CAD38695.1.
RefSeqNP_079366.3. NM_025090.3.
XP_005257599.1. XM_005257542.1.
XP_005257600.1. XM_005257543.1.
XP_005257601.1. XM_005257544.1.
UniGeneHs.464243.

3D structure databases

ProteinModelPortalQ9P275.
SMRQ9P275. Positions 122-420.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121651. 26 interactions.
IntActQ9P275. 23 interactions.
MINTMINT-4728276.
STRING9606.ENSP00000310590.

Protein family/group databases

MEROPSC19.042.

PTM databases

PhosphoSiteQ9P275.

Polymorphism databases

DMDM124056592.

2D gel databases

SWISS-2DPAGEQ9P275.

Proteomic databases

PaxDbQ9P275.
PeptideAtlasQ9P275.
PRIDEQ9P275.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000312010; ENSP00000310590; ENSG00000055483. [Q9P275-2]
ENST00000542802; ENSP00000441214; ENSG00000055483. [Q9P275-2]
ENST00000589225; ENSP00000467280; ENSG00000055483. [Q9P275-2]
GeneID57602.
KEGGhsa:57602.
UCSCuc002jvz.1. human. [Q9P275-2]
uc002jwc.1. human. [Q9P275-1]

Organism-specific databases

CTD57602.
GeneCardsGC17M076792.
H-InvDBHIX0014226.
HGNCHGNC:20062. USP36.
HPAHPA012082.
HPA052860.
MIM612543. gene.
neXtProtNX_Q9P275.
PharmGKBPA134949090.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000154759.
HOVERGENHBG061810.
InParanoidQ9P275.
KOK11855.
OMAHQDPPWH.
PhylomeDBQ9P275.
TreeFamTF315281.

Gene expression databases

ArrayExpressQ9P275.
BgeeQ9P275.
CleanExHS_USP36.
GenevestigatorQ9P275.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiUSP36.
GenomeRNAi57602.
NextBio64221.
PMAP-CutDBQ9P275.
PROQ9P275.
SOURCESearch...

Entry information

Entry nameUBP36_HUMAN
AccessionPrimary (citable) accession number: Q9P275
Secondary accession number(s): Q05C98 expand/collapse secondary AC list , Q05DD0, Q6IQ38, Q8NDM8, Q9NVC8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM