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Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

USP36

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei131NucleophilePROSITE-ProRule annotation1
Active sitei382Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • thiol-dependent ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  • positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
  • protein deubiquitination Source: FlyBase
  • regulation of mitophagy Source: ParkinsonsUK-UCL
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:HS00684-MONOMER.

Protein family/group databases

MEROPSiC19.042.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:USP36
Synonyms:KIAA1453
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:20062. USP36.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi57602.
OpenTargetsiENSG00000055483.
PharmGKBiPA134949090.

Polymorphism and mutation databases

BioMutaiUSP36.
DMDMi124056592.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806661 – 1121Ubiquitin carboxyl-terminal hydrolase 36Add BLAST1121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei429PhosphoserineCombined sources1
Modified residuei464PhosphoserineCombined sources1
Modified residuei546PhosphoserineCombined sources1
Modified residuei582PhosphoserineCombined sources1
Modified residuei667PhosphoserineCombined sources1
Modified residuei682PhosphoserineCombined sources1
Modified residuei713PhosphoserineCombined sources1
Modified residuei742PhosphoserineCombined sources1
Modified residuei952PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9P275.
MaxQBiQ9P275.
PaxDbiQ9P275.
PeptideAtlasiQ9P275.
PRIDEiQ9P275.

2D gel databases

SWISS-2DPAGEQ9P275.

PTM databases

iPTMnetiQ9P275.
PhosphoSitePlusiQ9P275.

Miscellaneous databases

PMAP-CutDBQ9P275.

Expressioni

Tissue specificityi

Broadly expressed.1 Publication

Gene expression databases

BgeeiENSG00000055483.
CleanExiHS_USP36.
ExpressionAtlasiQ9P275. baseline and differential.
GenevisibleiQ9P275. HS.

Organism-specific databases

HPAiHPA012082.
HPA012395.
HPA052860.

Interactioni

Subunit structurei

Interacts with PAF1.By similarity

Protein-protein interaction databases

BioGridi121651. 37 interactors.
IntActiQ9P275. 27 interactors.
MINTiMINT-4728276.
STRINGi9606.ENSP00000310590.

Structurei

3D structure databases

ProteinModelPortaliQ9P275.
SMRiQ9P275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini122 – 423USPAdd BLAST302

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1865. Eukaryota.
ENOG410XQ92. LUCA.
GeneTreeiENSGT00860000133708.
HOGENOMiHOG000154759.
HOVERGENiHBG061810.
InParanoidiQ9P275.
KOiK11855.
OMAiHQDPPWH.
OrthoDBiEOG091G02D8.
PhylomeDBiQ9P275.
TreeFamiTF315281.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P275-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPIVDKLKEA LKPGRKDSAD DGELGKLLAS SAKKVLLQKI EFEPASKSFS
60 70 80 90 100
YQLEALKSKY VLLNPKTEGA SRHKSGDDPP ARRQGSEHTY ESCGDGVPAP
110 120 130 140 150
QKVLFPTERL SLRWERVFRV GAGLHNLGNT CFLNATIQCL TYTPPLANYL
160 170 180 190 200
LSKEHARSCH QGSFCMLCVM QNHIVQAFAN SGNAIKPVSF IRDLKKIARH
210 220 230 240 250
FRFGNQEDAH EFLRYTIDAM QKACLNGCAK LDRQTQATTL VHQIFGGYLR
260 270 280 290 300
SRVKCSVCKS VSDTYDPYLD VALEIRQAAN IVRALELFVK ADVLSGENAY
310 320 330 340 350
MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN
360 370 380 390 400
IRPYMSQNNG DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS
410 420 430 440 450
LVHSSNVKVV LNQQAYVLFY LRIPGSKKSP EGLISRTGSS SLPGRPSVIP
460 470 480 490 500
DHSKKNIGNG IISSPLTGKR QDSGTMKKPH TTEEIGVPIS RNGSTLGLKS
510 520 530 540 550
QNGCIPPKLP SGSPSPKLSQ TPTHMPTILD DPGKKVKKPA PPQHFSPRTA
560 570 580 590 600
QGLPGTSNSN SSRSGSQRQG SWDSRDVVLS TSPKLLATAT ANGHGLKGND
610 620 630 640 650
ESAGLDRRGS SSSSPEHSAS SDSTKAPQTP RSGAAHLCDS QETNCSTAGH
660 670 680 690 700
SKTPPSGADS KTVKLKSPVL SNTTTEPAST MSPPPAKKLA LSAKKASTLW
710 720 730 740 750
RATGNDLRPP PPSPSSDLTH PMKTSHPVVA STWPVHRARA VSPAPQSSSR
760 770 780 790 800
LQPPFSPHPT LLSSTPKPPG TSEPRSCSSI STALPQVNED LVSLPHQLPE
810 820 830 840 850
ASEPPQSPSE KRKKTFVGEP QRLGSETRLP QHIREATAAP HGKRKRKKKK
860 870 880 890 900
RPEDTAASAL QEGQTQRQPG SPMYRREGQA QLPAVRRQED GTQPQVNGQQ
910 920 930 940 950
VGCVTDGHHA SSRKRRRKGA EGLGEEGGLH QDPLRHSCSP MGDGDPEAME
960 970 980 990 1000
ESPRKKKKRK QETQRAVEED GHLKCPRSAK PQDAVVPESS SCAPSANGWC
1010 1020 1030 1040 1050
PGDRMGLSQA PPVSWNGERE SDVVQELLKY SSDKAYGRKV LTWDGKMSAV
1060 1070 1080 1090 1100
SQDAIEDSRQ ARTETVVDDW DEEFDRGKEK KIKKFKREKR RNFNAFQKLQ
1110 1120
TRRNFWSVTH PAKAASLSYR R
Length:1,121
Mass (Da):122,652
Last modified:January 23, 2007 - v3
Checksum:i23E11A75B20304B7
GO
Isoform 2 (identifier: Q9P275-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     958-958: K → KKK

Show »
Length:1,123
Mass (Da):122,908
Checksum:i65CD93C1B8655319
GO

Sequence cautioni

The sequence AAH16487 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA95977 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82R → G in AAH71582 (PubMed:15489334).Curated1
Sequence conflicti573D → G in BAA91825 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037277271V → I.1 PublicationCorresponds to variant rs3744793dbSNPEnsembl.1
Natural variantiVAR_037278489I → M.Corresponds to variant rs3744795dbSNPEnsembl.1
Natural variantiVAR_037279775R → Q.Corresponds to variant rs9889908dbSNPEnsembl.1
Natural variantiVAR_037280806Q → R.3 PublicationsCorresponds to variant rs3088040dbSNPEnsembl.1
Natural variantiVAR_037281814K → N.Corresponds to variant rs3744797dbSNPEnsembl.1
Natural variantiVAR_037282828R → C.2 PublicationsCorresponds to variant rs1057040dbSNPEnsembl.1
Natural variantiVAR_058034887R → P.1 PublicationCorresponds to variant rs61760231dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024382958K → KKK in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040886 mRNA. Translation: BAA95977.1. Different initiation.
BC016487 mRNA. Translation: AAH16487.1. Sequence problems.
BC027992 mRNA. Translation: AAH27992.1.
BC071582 mRNA. Translation: AAH71582.1.
AK001671 mRNA. Translation: BAA91825.1.
AL833835 mRNA. Translation: CAD38695.1.
CCDSiCCDS32755.1. [Q9P275-2]
RefSeqiNP_001308220.1. NM_001321291.1. [Q9P275-2]
UniGeneiHs.464243.

Genome annotation databases

EnsembliENST00000312010; ENSP00000310590; ENSG00000055483. [Q9P275-2]
ENST00000542802; ENSP00000441214; ENSG00000055483. [Q9P275-2]
ENST00000589225; ENSP00000467280; ENSG00000055483. [Q9P275-2]
GeneIDi57602.
KEGGihsa:57602.
UCSCiuc002jvz.2. human. [Q9P275-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040886 mRNA. Translation: BAA95977.1. Different initiation.
BC016487 mRNA. Translation: AAH16487.1. Sequence problems.
BC027992 mRNA. Translation: AAH27992.1.
BC071582 mRNA. Translation: AAH71582.1.
AK001671 mRNA. Translation: BAA91825.1.
AL833835 mRNA. Translation: CAD38695.1.
CCDSiCCDS32755.1. [Q9P275-2]
RefSeqiNP_001308220.1. NM_001321291.1. [Q9P275-2]
UniGeneiHs.464243.

3D structure databases

ProteinModelPortaliQ9P275.
SMRiQ9P275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121651. 37 interactors.
IntActiQ9P275. 27 interactors.
MINTiMINT-4728276.
STRINGi9606.ENSP00000310590.

Protein family/group databases

MEROPSiC19.042.

PTM databases

iPTMnetiQ9P275.
PhosphoSitePlusiQ9P275.

Polymorphism and mutation databases

BioMutaiUSP36.
DMDMi124056592.

2D gel databases

SWISS-2DPAGEQ9P275.

Proteomic databases

EPDiQ9P275.
MaxQBiQ9P275.
PaxDbiQ9P275.
PeptideAtlasiQ9P275.
PRIDEiQ9P275.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312010; ENSP00000310590; ENSG00000055483. [Q9P275-2]
ENST00000542802; ENSP00000441214; ENSG00000055483. [Q9P275-2]
ENST00000589225; ENSP00000467280; ENSG00000055483. [Q9P275-2]
GeneIDi57602.
KEGGihsa:57602.
UCSCiuc002jvz.2. human. [Q9P275-1]

Organism-specific databases

CTDi57602.
DisGeNETi57602.
GeneCardsiUSP36.
H-InvDBHIX0014226.
HGNCiHGNC:20062. USP36.
HPAiHPA012082.
HPA012395.
HPA052860.
MIMi612543. gene.
neXtProtiNX_Q9P275.
OpenTargetsiENSG00000055483.
PharmGKBiPA134949090.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1865. Eukaryota.
ENOG410XQ92. LUCA.
GeneTreeiENSGT00860000133708.
HOGENOMiHOG000154759.
HOVERGENiHBG061810.
InParanoidiQ9P275.
KOiK11855.
OMAiHQDPPWH.
OrthoDBiEOG091G02D8.
PhylomeDBiQ9P275.
TreeFamiTF315281.

Enzyme and pathway databases

BioCyciZFISH:HS00684-MONOMER.

Miscellaneous databases

ChiTaRSiUSP36. human.
GeneWikiiUSP36.
GenomeRNAii57602.
PMAP-CutDBQ9P275.
PROiQ9P275.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000055483.
CleanExiHS_USP36.
ExpressionAtlasiQ9P275. baseline and differential.
GenevisibleiQ9P275. HS.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP36_HUMAN
AccessioniPrimary (citable) accession number: Q9P275
Secondary accession number(s): Q05C98
, Q05DD0, Q6IQ38, Q8NDM8, Q9NVC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.