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Q9P275

- UBP36_HUMAN

UniProt

Q9P275 - UBP36_HUMAN

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Protein
Ubiquitin carboxyl-terminal hydrolase 36
Gene
USP36, KIAA1453
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311Nucleophile By similarity
Active sitei382 – 3821Proton acceptor By similarity

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB
  2. ubiquitin-specific protease activity Source: FlyBase

GO - Biological processi

  1. protein deubiquitination Source: FlyBase
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.042.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene namesi
Name:USP36
Synonyms:KIAA1453
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:20062. USP36.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: HPA
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134949090.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11211121Ubiquitin carboxyl-terminal hydrolase 36
PRO_0000080666Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei464 – 4641Phosphoserine1 Publication
Modified residuei582 – 5821Phosphoserine2 Publications
Modified residuei667 – 6671Phosphoserine2 Publications
Modified residuei682 – 6821Phosphoserine1 Publication
Modified residuei713 – 7131Phosphoserine1 Publication
Modified residuei742 – 7421Phosphoserine2 Publications
Modified residuei952 – 9521Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P275.
PaxDbiQ9P275.
PeptideAtlasiQ9P275.
PRIDEiQ9P275.

2D gel databases

SWISS-2DPAGEQ9P275.

PTM databases

PhosphoSiteiQ9P275.

Miscellaneous databases

PMAP-CutDBQ9P275.

Expressioni

Tissue specificityi

Broadly expressed.1 Publication

Gene expression databases

ArrayExpressiQ9P275.
BgeeiQ9P275.
CleanExiHS_USP36.
GenevestigatoriQ9P275.

Organism-specific databases

HPAiHPA012082.
HPA052860.

Interactioni

Subunit structurei

Interacts with PAF1 By similarity.

Protein-protein interaction databases

BioGridi121651. 26 interactions.
IntActiQ9P275. 23 interactions.
MINTiMINT-4728276.
STRINGi9606.ENSP00000310590.

Structurei

3D structure databases

ProteinModelPortaliQ9P275.
SMRiQ9P275. Positions 122-420.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 423302USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000154759.
HOVERGENiHBG061810.
InParanoidiQ9P275.
KOiK11855.
OMAiHQDPPWH.
PhylomeDBiQ9P275.
TreeFamiTF315281.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9P275-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPIVDKLKEA LKPGRKDSAD DGELGKLLAS SAKKVLLQKI EFEPASKSFS     50
YQLEALKSKY VLLNPKTEGA SRHKSGDDPP ARRQGSEHTY ESCGDGVPAP 100
QKVLFPTERL SLRWERVFRV GAGLHNLGNT CFLNATIQCL TYTPPLANYL 150
LSKEHARSCH QGSFCMLCVM QNHIVQAFAN SGNAIKPVSF IRDLKKIARH 200
FRFGNQEDAH EFLRYTIDAM QKACLNGCAK LDRQTQATTL VHQIFGGYLR 250
SRVKCSVCKS VSDTYDPYLD VALEIRQAAN IVRALELFVK ADVLSGENAY 300
MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN 350
IRPYMSQNNG DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS 400
LVHSSNVKVV LNQQAYVLFY LRIPGSKKSP EGLISRTGSS SLPGRPSVIP 450
DHSKKNIGNG IISSPLTGKR QDSGTMKKPH TTEEIGVPIS RNGSTLGLKS 500
QNGCIPPKLP SGSPSPKLSQ TPTHMPTILD DPGKKVKKPA PPQHFSPRTA 550
QGLPGTSNSN SSRSGSQRQG SWDSRDVVLS TSPKLLATAT ANGHGLKGND 600
ESAGLDRRGS SSSSPEHSAS SDSTKAPQTP RSGAAHLCDS QETNCSTAGH 650
SKTPPSGADS KTVKLKSPVL SNTTTEPAST MSPPPAKKLA LSAKKASTLW 700
RATGNDLRPP PPSPSSDLTH PMKTSHPVVA STWPVHRARA VSPAPQSSSR 750
LQPPFSPHPT LLSSTPKPPG TSEPRSCSSI STALPQVNED LVSLPHQLPE 800
ASEPPQSPSE KRKKTFVGEP QRLGSETRLP QHIREATAAP HGKRKRKKKK 850
RPEDTAASAL QEGQTQRQPG SPMYRREGQA QLPAVRRQED GTQPQVNGQQ 900
VGCVTDGHHA SSRKRRRKGA EGLGEEGGLH QDPLRHSCSP MGDGDPEAME 950
ESPRKKKKRK QETQRAVEED GHLKCPRSAK PQDAVVPESS SCAPSANGWC 1000
PGDRMGLSQA PPVSWNGERE SDVVQELLKY SSDKAYGRKV LTWDGKMSAV 1050
SQDAIEDSRQ ARTETVVDDW DEEFDRGKEK KIKKFKREKR RNFNAFQKLQ 1100
TRRNFWSVTH PAKAASLSYR R 1121
Length:1,121
Mass (Da):122,652
Last modified:January 23, 2007 - v3
Checksum:i23E11A75B20304B7
GO
Isoform 2 (identifier: Q9P275-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     958-958: K → KKK

Show »
Length:1,123
Mass (Da):122,908
Checksum:i65CD93C1B8655319
GO

Sequence cautioni

The sequence AAH16487.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
The sequence BAA95977.1 differs from that shown. Reason: Erroneous initiation.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti271 – 2711V → I.1 Publication
Corresponds to variant rs3744793 [ dbSNP | Ensembl ].
VAR_037277
Natural varianti489 – 4891I → M.
Corresponds to variant rs3744795 [ dbSNP | Ensembl ].
VAR_037278
Natural varianti775 – 7751R → Q.
Corresponds to variant rs9889908 [ dbSNP | Ensembl ].
VAR_037279
Natural varianti806 – 8061Q → R.3 Publications
Corresponds to variant rs3088040 [ dbSNP | Ensembl ].
VAR_037280
Natural varianti814 – 8141K → N.
Corresponds to variant rs3744797 [ dbSNP | Ensembl ].
VAR_037281
Natural varianti828 – 8281R → C.2 Publications
Corresponds to variant rs1057040 [ dbSNP | Ensembl ].
VAR_037282
Natural varianti887 – 8871R → P.1 Publication
Corresponds to variant rs61760231 [ dbSNP | Ensembl ].
VAR_058034

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei958 – 9581K → KKK in isoform 2.
VSP_024382

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821R → G in AAH71582. 1 Publication
Sequence conflicti573 – 5731D → G in BAA91825. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040886 mRNA. Translation: BAA95977.1. Different initiation.
BC016487 mRNA. Translation: AAH16487.1. Sequence problems.
BC027992 mRNA. Translation: AAH27992.1.
BC071582 mRNA. Translation: AAH71582.1.
AK001671 mRNA. Translation: BAA91825.1.
AL833835 mRNA. Translation: CAD38695.1.
CCDSiCCDS32755.1. [Q9P275-2]
RefSeqiNP_079366.3. NM_025090.3. [Q9P275-2]
UniGeneiHs.464243.

Genome annotation databases

EnsembliENST00000312010; ENSP00000310590; ENSG00000055483. [Q9P275-2]
ENST00000542802; ENSP00000441214; ENSG00000055483. [Q9P275-2]
ENST00000589225; ENSP00000467280; ENSG00000055483. [Q9P275-2]
GeneIDi57602.
KEGGihsa:57602.
UCSCiuc002jvz.1. human. [Q9P275-2]
uc002jwc.1. human. [Q9P275-1]

Polymorphism databases

DMDMi124056592.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB040886 mRNA. Translation: BAA95977.1 . Different initiation.
BC016487 mRNA. Translation: AAH16487.1 . Sequence problems.
BC027992 mRNA. Translation: AAH27992.1 .
BC071582 mRNA. Translation: AAH71582.1 .
AK001671 mRNA. Translation: BAA91825.1 .
AL833835 mRNA. Translation: CAD38695.1 .
CCDSi CCDS32755.1. [Q9P275-2 ]
RefSeqi NP_079366.3. NM_025090.3. [Q9P275-2 ]
UniGenei Hs.464243.

3D structure databases

ProteinModelPortali Q9P275.
SMRi Q9P275. Positions 122-420.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 121651. 26 interactions.
IntActi Q9P275. 23 interactions.
MINTi MINT-4728276.
STRINGi 9606.ENSP00000310590.

Protein family/group databases

MEROPSi C19.042.

PTM databases

PhosphoSitei Q9P275.

Polymorphism databases

DMDMi 124056592.

2D gel databases

SWISS-2DPAGE Q9P275.

Proteomic databases

MaxQBi Q9P275.
PaxDbi Q9P275.
PeptideAtlasi Q9P275.
PRIDEi Q9P275.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000312010 ; ENSP00000310590 ; ENSG00000055483 . [Q9P275-2 ]
ENST00000542802 ; ENSP00000441214 ; ENSG00000055483 . [Q9P275-2 ]
ENST00000589225 ; ENSP00000467280 ; ENSG00000055483 . [Q9P275-2 ]
GeneIDi 57602.
KEGGi hsa:57602.
UCSCi uc002jvz.1. human. [Q9P275-2 ]
uc002jwc.1. human. [Q9P275-1 ]

Organism-specific databases

CTDi 57602.
GeneCardsi GC17M076792.
H-InvDB HIX0014226.
HGNCi HGNC:20062. USP36.
HPAi HPA012082.
HPA052860.
MIMi 612543. gene.
neXtProti NX_Q9P275.
PharmGKBi PA134949090.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000154759.
HOVERGENi HBG061810.
InParanoidi Q9P275.
KOi K11855.
OMAi HQDPPWH.
PhylomeDBi Q9P275.
TreeFami TF315281.

Miscellaneous databases

GeneWikii USP36.
GenomeRNAii 57602.
NextBioi 64221.
PMAP-CutDB Q9P275.
PROi Q9P275.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9P275.
Bgeei Q9P275.
CleanExi HS_USP36.
Genevestigatori Q9P275.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
    DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ARG-806 AND CYS-828.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ILE-271; ARG-806 AND PRO-887.
    Tissue: Cervix, Placenta and Skin.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-954 (ISOFORM 1), VARIANTS ARG-806 AND CYS-828.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 494-1121 (ISOFORM 2).
    Tissue: Testis.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases."
    Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C.
    Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, ENZYME ACTIVITY.
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-952, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464; SER-582 AND SER-667, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-667 AND SER-682, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582; SER-713; SER-742 AND SER-952, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP36_HUMAN
AccessioniPrimary (citable) accession number: Q9P275
Secondary accession number(s): Q05C98
, Q05DD0, Q6IQ38, Q8NDM8, Q9NVC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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