Q9P275 (UBP36_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 36
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 36
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36

Gene names

Name:	USP36
Synonyms:	KIAA1453

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1121 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.6
Subunit structure	Interacts with PAF1 By similarity.
Subcellular location	Nucleus › nucleolus Ref.5.
Tissue specificity	Broadly expressed. Ref.6
Sequence similarities	Belongs to the peptidase C19 family.
Sequence caution	The sequence AAH16487.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence BAA95977.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleolus Inferred from direct assay. Source: HPA
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P275-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P275-2)

The sequence of this isoform differs from the canonical sequence as follows:
     958-958: K → KKK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1121	1121	Ubiquitin carboxyl-terminal hydrolase 36		PRO_0000080666
Sites
	Active site	131	1	Nucleophile By similarity
	Active site	382	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	464	1	Phosphoserine Ref.8
	Modified residue	582	1	Phosphoserine Ref.8 Ref.10
	Modified residue	667	1	Phosphoserine Ref.8 Ref.9
	Modified residue	682	1	Phosphoserine Ref.9
	Modified residue	713	1	Phosphoserine Ref.10
	Modified residue	742	1	Phosphoserine Ref.10 Ref.11
	Modified residue	952	1	Phosphoserine Ref.7 Ref.10
Natural variations
	Alternative sequence	958	1	K → KKK in isoform 2.		VSP_024382
	Natural variant	271	1	V → I. Ref.2 Corresponds to variant rs3744793 [ dbSNP | Ensembl ].		VAR_037277
	Natural variant	489	1	I → M. Corresponds to variant rs3744795 [ dbSNP | Ensembl ].		VAR_037278
	Natural variant	775	1	R → Q. Corresponds to variant rs9889908 [ dbSNP | Ensembl ].		VAR_037279
	Natural variant	806	1	Q → R. Ref.1 Ref.2 Ref.3 Corresponds to variant rs3088040 [ dbSNP | Ensembl ].		VAR_037280
	Natural variant	814	1	K → N. Corresponds to variant rs3744797 [ dbSNP | Ensembl ].		VAR_037281
	Natural variant	828	1	R → C. Ref.1 Ref.3 Corresponds to variant rs1057040 [ dbSNP | Ensembl ].		VAR_037282
	Natural variant	887	1	R → P. Ref.2 Corresponds to variant rs61760231 [ dbSNP | Ensembl ].		VAR_058034
Experimental info
	Sequence conflict	82	1	R → G in AAH71582. Ref.2
	Sequence conflict	573	1	D → G in BAA91825. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 23E11A75B20304B7	FASTA	1,121	122,652
	10 20 30 40 50 60 MPIVDKLKEA LKPGRKDSAD DGELGKLLAS SAKKVLLQKI EFEPASKSFS YQLEALKSKY 70 80 90 100 110 120 VLLNPKTEGA SRHKSGDDPP ARRQGSEHTY ESCGDGVPAP QKVLFPTERL SLRWERVFRV 130 140 150 160 170 180 GAGLHNLGNT CFLNATIQCL TYTPPLANYL LSKEHARSCH QGSFCMLCVM QNHIVQAFAN 190 200 210 220 230 240 SGNAIKPVSF IRDLKKIARH FRFGNQEDAH EFLRYTIDAM QKACLNGCAK LDRQTQATTL 250 260 270 280 290 300 VHQIFGGYLR SRVKCSVCKS VSDTYDPYLD VALEIRQAAN IVRALELFVK ADVLSGENAY 310 320 330 340 350 360 MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN IRPYMSQNNG 370 380 390 400 410 420 DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS LVHSSNVKVV LNQQAYVLFY 430 440 450 460 470 480 LRIPGSKKSP EGLISRTGSS SLPGRPSVIP DHSKKNIGNG IISSPLTGKR QDSGTMKKPH 490 500 510 520 530 540 TTEEIGVPIS RNGSTLGLKS QNGCIPPKLP SGSPSPKLSQ TPTHMPTILD DPGKKVKKPA 550 560 570 580 590 600 PPQHFSPRTA QGLPGTSNSN SSRSGSQRQG SWDSRDVVLS TSPKLLATAT ANGHGLKGND 610 620 630 640 650 660 ESAGLDRRGS SSSSPEHSAS SDSTKAPQTP RSGAAHLCDS QETNCSTAGH SKTPPSGADS 670 680 690 700 710 720 KTVKLKSPVL SNTTTEPAST MSPPPAKKLA LSAKKASTLW RATGNDLRPP PPSPSSDLTH 730 740 750 760 770 780 PMKTSHPVVA STWPVHRARA VSPAPQSSSR LQPPFSPHPT LLSSTPKPPG TSEPRSCSSI 790 800 810 820 830 840 STALPQVNED LVSLPHQLPE ASEPPQSPSE KRKKTFVGEP QRLGSETRLP QHIREATAAP 850 860 870 880 890 900 HGKRKRKKKK RPEDTAASAL QEGQTQRQPG SPMYRREGQA QLPAVRRQED GTQPQVNGQQ 910 920 930 940 950 960 VGCVTDGHHA SSRKRRRKGA EGLGEEGGLH QDPLRHSCSP MGDGDPEAME ESPRKKKKRK 970 980 990 1000 1010 1020 QETQRAVEED GHLKCPRSAK PQDAVVPESS SCAPSANGWC PGDRMGLSQA PPVSWNGERE 1030 1040 1050 1060 1070 1080 SDVVQELLKY SSDKAYGRKV LTWDGKMSAV SQDAIEDSRQ ARTETVVDDW DEEFDRGKEK 1090 1100 1110 1120 KIKKFKREKR RNFNAFQKLQ TRRNFWSVTH PAKAASLSYR R
	Isoform 2 [UniParc]. Checksum: 65CD93C1B8655319	FASTA	1,123	122,908
	10 20 30 40 50 60 MPIVDKLKEA LKPGRKDSAD DGELGKLLAS SAKKVLLQKI EFEPASKSFS YQLEALKSKY 70 80 90 100 110 120 VLLNPKTEGA SRHKSGDDPP ARRQGSEHTY ESCGDGVPAP QKVLFPTERL SLRWERVFRV 130 140 150 160 170 180 GAGLHNLGNT CFLNATIQCL TYTPPLANYL LSKEHARSCH QGSFCMLCVM QNHIVQAFAN 190 200 210 220 230 240 SGNAIKPVSF IRDLKKIARH FRFGNQEDAH EFLRYTIDAM QKACLNGCAK LDRQTQATTL 250 260 270 280 290 300 VHQIFGGYLR SRVKCSVCKS VSDTYDPYLD VALEIRQAAN IVRALELFVK ADVLSGENAY 310 320 330 340 350 360 MCAKCKKKVP ASKRFTIHRT SNVLTLSLKR FANFSGGKIT KDVGYPEFLN IRPYMSQNNG 370 380 390 400 410 420 DPVMYGLYAV LVHSGYSCHA GHYYCYVKAS NGQWYQMNDS LVHSSNVKVV LNQQAYVLFY 430 440 450 460 470 480 LRIPGSKKSP EGLISRTGSS SLPGRPSVIP DHSKKNIGNG IISSPLTGKR QDSGTMKKPH 490 500 510 520 530 540 TTEEIGVPIS RNGSTLGLKS QNGCIPPKLP SGSPSPKLSQ TPTHMPTILD DPGKKVKKPA 550 560 570 580 590 600 PPQHFSPRTA QGLPGTSNSN SSRSGSQRQG SWDSRDVVLS TSPKLLATAT ANGHGLKGND 610 620 630 640 650 660 ESAGLDRRGS SSSSPEHSAS SDSTKAPQTP RSGAAHLCDS QETNCSTAGH SKTPPSGADS 670 680 690 700 710 720 KTVKLKSPVL SNTTTEPAST MSPPPAKKLA LSAKKASTLW RATGNDLRPP PPSPSSDLTH 730 740 750 760 770 780 PMKTSHPVVA STWPVHRARA VSPAPQSSSR LQPPFSPHPT LLSSTPKPPG TSEPRSCSSI 790 800 810 820 830 840 STALPQVNED LVSLPHQLPE ASEPPQSPSE KRKKTFVGEP QRLGSETRLP QHIREATAAP 850 860 870 880 890 900 HGKRKRKKKK RPEDTAASAL QEGQTQRQPG SPMYRREGQA QLPAVRRQED GTQPQVNGQQ 910 920 930 940 950 960 VGCVTDGHHA SSRKRRRKGA EGLGEEGGLH QDPLRHSCSP MGDGDPEAME ESPRKKKKKK 970 980 990 1000 1010 1020 RKQETQRAVE EDGHLKCPRS AKPQDAVVPE SSSCAPSANG WCPGDRMGLS QAPPVSWNGE 1030 1040 1050 1060 1070 1080 RESDVVQELL KYSSDKAYGR KVLTWDGKMS AVSQDAIEDS RQARTETVVD DWDEEFDRGK 1090 1100 1110 1120 EKKIKKFKRE KRRNFNAFQK LQTRRNFWSV THPAKAASLS YRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ARG-806 AND CYS-828. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ILE-271; ARG-806 AND PRO-887. Tissue: Cervix, Placenta and Skin.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-954 (ISOFORM 1), VARIANTS ARG-806 AND CYS-828.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 494-1121 (ISOFORM 2). Tissue: Testis.
[5]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases." Quesada V., Diaz-Perales A., Gutierrez-Fernandez A., Garabaya C., Cal S., Lopez-Otin C. Biochem. Biophys. Res. Commun. 314:54-62(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, ENZYME ACTIVITY.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-952, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-464; SER-582 AND SER-667, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-667 AND SER-682, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582; SER-713; SER-742 AND SER-952, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-742, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB040886 mRNA. Translation: BAA95977.1. Different initiation. BC016487 mRNA. Translation: AAH16487.1. Sequence problems. BC027992 mRNA. Translation: AAH27992.1. BC071582 mRNA. Translation: AAH71582.1. AK001671 mRNA. Translation: BAA91825.1. AL833835 mRNA. Translation: CAD38695.1.
IPI	IPI00001786. IPI00843790.
RefSeq	NP_079366.3. NM_025090.3.
UniGene	Hs.464243.
3D structure databases
ProteinModelPortal	Q9P275.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P275. 23 interactions.
STRING	9606.ENSP00000310590.
Protein family/group databases
MEROPS	C19.042.
PTM databases
PhosphoSite	Q9P275.
Polymorphism databases
DMDM	124056592.
2D gel databases
SWISS-2DPAGE	Q9P275.
Proteomic databases
PaxDb	Q9P275.
PeptideAtlas	Q9P275.
PRIDE	Q9P275.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000312010; ENSP00000310590; ENSG00000055483. ENST00000542802; ENSP00000441214; ENSG00000055483. ENST00000589225; ENSP00000467280; ENSG00000055483.
GeneID	57602.
KEGG	hsa:57602.
UCSC	uc002jvz.1. human. uc002jwc.1. human.
Organism-specific databases
CTD	57602.
GeneCards	GC17M076792.
H-InvDB	HIX0014226.
HGNC	HGNC:20062. USP36.
HPA	HPA012082.
MIM	612543. gene.
neXtProt	NX_Q9P275.
PharmGKB	PA134949090.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5533.
HOGENOM	HOG000154759.
HOVERGEN	HBG061810.
InParanoid	Q9P275.
KO	K11855.
OMA	VTHPAKA.
OrthoDB	EOG4V9TQ1.
Gene expression databases
ArrayExpress	Q9P275.
Bgee	Q9P275.
CleanEx	HS_USP36.
Genevestigator	Q9P275.
GermOnline	ENSG00000055483. Homo sapiens.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	57602.
NextBio	64221.
PMAP-CutDB	Q9P275.
SOURCE	Search...

Entry information

Entry name

UBP36_HUMAN

Accession

Primary (citable) accession number: Q9P275
Secondary accession number(s): Q05C98 , Q05DD0, Q6IQ38, Q8NDM8, Q9NVC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.