Q9P270 (SLAI2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SLAIN motif-containing protein 2

Gene names

Name:	SLAIN2
Synonyms:	KIAA1458

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Required for normal structure of the microtubule cytoskeleton during interphase. Ref.9
Subunit structure	Interacts with CLIP1, CLIP2, CKAP5, CLASP1, MAPRE1 and MAPRE3. Ref.9
Subcellular location	Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. Detected at the plus end of growing microtubules.
Tissue specificity	Widely expressed with highest levels in adult liver, testis and ovary, and lowest levels in adult pancreas and spleen and in fetal brain. Ref.1
Domain	The N-terminus forms a two-stranded coiled coil. Ref.9
Post-translational modification	Is highly phosphorylated during mitosis, but not during interphase. The highly phosphorylated form does not localize at microtubule plus ends and does not interact with MAPRE1 or CKAP5. Ref.9
Sequence similarities	Belongs to the SLAIN motif-containing family.
Sequence caution	The sequence AAH31691.2 differs from that shown. Reason: Erroneous initiation. The sequence BAA95982.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic microtubule organization Inferred from mutant phenotype Ref.9. Source: UniProtKB microtubule nucleation Inferred from mutant phenotype Ref.9. Source: UniProtKB positive regulation of microtubule polymerization Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular_component	centrosome Inferred from direct assay PubMed 21399614. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 581

581

SLAIN motif-containing protein 2

PRO_0000316965

Regions

Coiled coil

4 – 39

36

Ref.9

Compositional bias

124 – 128

5

Poly-Glu

Sites

Site

581

1

Important for interaction with CLIP1

Amino acid modifications

Modified residue

43

1

Phosphoserine Ref.4 Ref.5

Modified residue

48

1

Phosphoserine Ref.4 Ref.5

Modified residue

63

1

Phosphoserine Ref.5

Modified residue

87

1

Phosphoserine By similarity

Modified residue

147

1

Phosphoserine Ref.5

Modified residue

160

1

Phosphoserine Ref.4

Modified residue

179

1

Phosphoserine Ref.5

Modified residue

247

1

Phosphoserine Ref.5

Modified residue

250

1

Phosphoserine Ref.5

Modified residue

251

1

Phosphoserine Ref.5

Modified residue

254

1

Phosphoserine Ref.5

Modified residue

323

1

Phosphoserine Ref.5

Modified residue

377

1

Phosphoserine Ref.5

Modified residue

391

1

Phosphoserine Ref.7

Modified residue

413

1

Phosphoserine Ref.5

Modified residue

433

1

Phosphoserine Ref.6

Modified residue

456

1

Phosphoserine Ref.5

Modified residue

462

1

Phosphoserine Ref.5 Ref.7

Modified residue

467

1

Phosphoserine Ref.5 Ref.7

Experimental info

Mutagenesis

581

1

Missing: Abolishes interaction with CLIP1. Ref.9

Sequence conflict

233

1

N → D in BAF83695. Ref.2

Sequence conflict

558

1

V → A in BAF83695. Ref.2

Secondary structure

1

.

.

.

581

Helix Strand Turn

Details...

Helix

575 – 577

3

Sequences

	Sequence		Length	Mass (Da)
	Q9P270 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: 44B10B1BBD555B26	FASTA	581	62,543
	10 20 30 40 50 60 MEDVNSNVNA DQEVRKLQEL VKKLEKQNEQ LRSRSGAVQG AGSLGPGSPV RAGASIPSSG 70 80 90 100 110 120 AASPRGFPLG LSAKSGGGPG SGPRRTSSEE LRDATSLLAA GEGGLLDEVE PLRPDELERL 130 140 150 160 170 180 SGWEEEEESW LYSSPKKKLT PMQKSVSPLV WCRQVLDYPS PDVECAKKSL IHKLDQTMSA 190 200 210 220 230 240 LKRQNLYNNP FNSMSYTSPY SPNASSPYSS GFNSPSSTPV RPPIVKQLIL PGNSGNLKSS 250 260 270 280 290 300 DRNPPLSPQS SIDSELSASE LDEDSIGSNY KLNDVTDVQI LARMQEESLR QEYAATTSRR 310 320 330 340 350 360 SSGSSCNSTR RGTFSDQELD AQSLDDEDDN MHHAVYPAVN RFSPSPRNSP RPSPKQSPRN 370 380 390 400 410 420 SPRSRSPARG IEYSRVSPQP MISRLQQPRL SLQGHPTDLQ TSNVKNEEKL RRSLPNLSRT 430 440 450 460 470 480 SNTQVDSVKS SRSDSNFQVP NGGIPRMQPQ ASAIPSPGKF RSPAAPSPLA LRQPVKAFSN 490 500 510 520 530 540 HGSGSPGSQE ITQLTQTTSS PGPPMVQSTV SANPPSNINS ATLTRPAGTT AMRSGLPRPS 550 560 570 580 APSAGGIPVP RSKLAQPVRR SLPAPKTYGS MKDDSWKDGC Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-581. Tissue: Skin.
[4]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-48 AND SER-160, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-48; SER-63; SER-147; SER-179; SER-247; SER-250; SER-251; SER-254; SER-323; SER-377; SER-413; SER-456; SER-462 AND SER-467, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-433, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-391; SER-462 AND SER-467, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase." van der Vaart B., Manatschal C., Grigoriev I., Olieric V., Gouveia S.M., Bjelic S., Demmers J., Vorobjev I., Hoogenraad C.C., Steinmetz M.O., Akhmanova A. J. Cell Biol. 193:1083-1099(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 574-581 IN COMPLEX WITH CLIP1, FUNCTION, CIRCULAR DICHROISM, COILED-COIL DOMAIN, INTERACTION WITH CLIP1; CLIP2; CLASP1; CKAP5; MAPRE1 AND MAPRE3, PHOSPHORYLATION, MUTAGENESIS OF TYR-581, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB040891 mRNA. Translation: BAA95982.1. Different initiation.
AK291006 mRNA. Translation: BAF83695.1.
BC031691 mRNA. Translation: AAH31691.2. Different initiation.

IPI

IPI00853278.

RefSeq

NP_065897.1. NM_020846.1.

UniGene

Hs.479677.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RDV	X-ray	1.75	E/F/G/H	574-581	[»]

ProteinModelPortal

Q9P270.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9P270. 2 interactions.

MINT

MINT-3306688.

STRING

9606.ENSP00000264313.

PTM databases

PhosphoSite

Q9P270.

Polymorphism databases

DMDM

166977679.

Proteomic databases

PaxDb

Q9P270.

PRIDE

Q9P270.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000264313; ENSP00000264313; ENSG00000109171.

GeneID

57606.

KEGG

hsa:57606.

UCSC

uc003gya.4. human.

Organism-specific databases

CTD

57606.

GeneCards

GC04P048343.

H-InvDB

HIX0012487.

HGNC

HGNC:29282. SLAIN2.

HPA

HPA035824.

MIM

610492. gene.

neXtProt

NX_Q9P270.

PharmGKB

PA162403457.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG29486.

HOGENOM

HOG000057277.

HOVERGEN

HBG072961.

InParanoid

Q9P270.

KO

K16580.

OMA

KRQNLYN.

OrthoDB

EOG4HX51B.

Gene expression databases

ArrayExpress

Q9P270.

Bgee

Q9P270.

CleanEx

HS_SLAIN2.

Genevestigator

Q9P270.

Family and domain databases

InterPro

IPR026179. Slain.
[Graphical view]

PANTHER

PTHR22406. PTHR22406. 1 hit.

ProtoNet

Search...

Other

ChiTaRS

SLAIN2. human.

GenomeRNAi

57606.

NextBio

64235.

SOURCE

Search...

Entry information

Entry name

SLAI2_HUMAN

Accession

Primary (citable) accession number: Q9P270
Secondary accession number(s): A8K4P1, Q8N5R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	April 3, 2013
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.