Q9P253 (VPS18_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 18 homolog
Short name=hVPS18

Gene names

Name:	VPS18
Synonyms:	KIAA1475

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	973 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Ref.5
Subunit structure	Interacts with HOOK1 By similarity. Part of a large heterooligomeric complex together with VPS11, VPS16 and VPS33A. Interacts with STX7. Ref.5
Subcellular location	Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes. Ref.5 Ref.7
Tissue specificity	Ubiquitous. Expression was highest in heart and low in lung. Ref.5
Sequence similarities	Belongs to the VPS18 family. Contains 1 clathrin repeat. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAH01513.1 differs from that shown. Reason: Unlikely isoform. Aberrant splice sites. The sequence BAA95999.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Lysosome Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endosome organization Inferred from direct assay. Source: UniProtKB intracellular protein transport Inferred from electronic annotation. Source: InterPro lysosome organization Inferred from direct assay. Source: UniProtKB vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	HOPS complex Inferred from direct assay. Source: UniProtKB late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MON1B	Q7L1V2	2	EBI-1053363,EBI-2655311

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 973	973	Vacuolar protein sorting-associated protein 18 homolog		PRO_0000055906
Regions
	Repeat	627 – 785	159	Clathrin
	Zinc finger	853 – 947	95	RING-type
	Coiled coil	454 – 481	28	Potential
	Coiled coil	802 – 848	47	Potential
Amino acid modifications
	Modified residue	362	1	N6-acetyllysine Ref.10
	Modified residue	528	1	Phosphoserine Ref.6
	Modified residue	529	1	Phosphoserine Ref.6
	Modified residue	689	1	Phosphoserine Ref.8 Ref.9
Natural variations
	Natural variant	913	1	A → S in a colorectal cancer sample; somatic mutation. Ref.12		VAR_035950

Sequences

	Sequence		Length	Mass (Da)
	Q9P253 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 0008E21D66E8CFEC	FASTA	973	110,186
	10 20 30 40 50 60 MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL 70 80 90 100 110 120 VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV HKMFLDHTGS HLLIALSSTE 130 140 150 160 170 180 VLYVNRNGQK VRPLARWKGQ LVESVGWNKA LGTESSTGPI LVGTAQGHIF EAELSASEGG 190 200 210 220 230 240 LFGPAPDLYF RPLYVLNEEG GPAPVCSLEA ERGPDGRSFV IATTRQRLFQ FIGRAAEGAE 250 260 270 280 290 300 AQGFSGLFAA YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGALDCG 310 320 330 340 350 360 RPDSLLSEER VWEYPEGVGP GASPPLAIVL TQFHFLLLLA DRVEAVCTLT GQVVLRDHFL 370 380 390 400 410 420 EKFGPLKHMV KDSSTGQLWA YTERAVFRYH VQREARDVWR TYLDMNRFDL AKEYCRERPD 430 440 450 460 470 480 CLDTVLAREA DFCFRQRRYL ESARCYALTQ SYFEEIALKF LEARQEEALA EFLQRKLASL 490 500 510 520 530 540 KPAERTQATL LTTWLTELYL SRLGALQGDP EALTLYRETK ECFRTFLSSP RHKEWLFASR 550 560 570 580 590 600 ASIHELLASH GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP 610 620 630 640 650 660 ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEVQ QVSQAIRYME FCVNVLGETE 670 680 690 700 710 720 QAIHNYLLSL YARGRPDSLL AYLEQAGASP HRVHYDLKYA LRLCAEHGHH RACVHVYKVL 730 740 750 760 770 780 ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE LRKKLWLKIA RHVVQEEEDV QTAMACLASC 790 800 810 820 830 840 PLLKIEDVLP FFPDFVTIDH FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL 850 860 870 880 890 900 RGRYGTVEPQ DKCATCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR 910 920 930 940 950 960 KLGAAPPPAK GSARAKEAEG GAATAGPSRE QLKADLDELV AAECVYCGEL MIRSIDRPFI 970 DPQRYEEEQL SWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of human VPS18, VPS11, VPS16, and VPS33." Huizing M., Didier A., Walenta J., Anikster Y., Gahl W.A., Kraemer H. Gene 264:241-247(2001) [PubMed: 11250079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed: 10819331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Melanoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Molecular characterization of mammalian homologues of class c Vps proteins that interact with syntaxin-7." Kim B.Y., Kraemer H., Yamamoto A., Kominami E., Kohsaka S., Akazawa C. J. Biol. Chem. 276:29393-29402(2001) [PubMed: 11382755] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-528 AND SER-529, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Integral and associated lysosomal membrane proteins." Schroeder B., Wrocklage C., Pan C., Jaeger R., Koesters B., Schaefer H., Elsaesser H.-P., Mann M., Hasilik A. Traffic 8:1676-1686(2007) [PubMed: 17897319] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Placenta.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-362, MASS SPECTROMETRY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-913.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF308802 mRNA. Translation: AAG34679.1. AB040908 mRNA. Translation: BAA95999.1. Different initiation. AL713725 mRNA. Translation: CAD28515.1. BC001513 mRNA. Translation: AAH01513.1. Sequence problems.
IPI	IPI00001985.
RefSeq	NP_065908.1. NM_020857.2.
UniGene	Hs.23876.
3D structure databases
ProteinModelPortal	Q9P253.
SMR	Q9P253. Positions 849-883.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P253. 5 interactions.
STRING	Q9P253.
PTM databases
PhosphoSite	Q9P253.
Polymorphism databases
DMDM	23396938.
Proteomic databases
PeptideAtlas	Q9P253.
PRIDE	Q9P253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000220509; ENSP00000220509; ENSG00000104142.
GeneID	57617.
KEGG	hsa:57617.
UCSC	uc001zne.1. human.
Organism-specific databases
CTD	57617.
GeneCards	GC15P041186.
H-InvDB	HIX0012143.
HGNC	HGNC:15972. VPS18.
HPA	HPA040701.
MIM	608551. gene.
neXtProt	NX_Q9P253.
PharmGKB	PA38069.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15255.
GeneTree	ENSGT00390000015233.
HOGENOM	HBG356873.
HOVERGEN	HBG057876.
InParanoid	Q9P253.
OMA	FYLFLCG.
OrthoDB	EOG4F4S9B.
PhylomeDB	Q9P253.
Gene expression databases
ArrayExpress	Q9P253.
Bgee	Q9P253.
CleanEx	HS_VPS18.
Genevestigator	Q9P253.
GermOnline	ENSG00000104142. Homo sapiens.
Family and domain databases
InterPro	IPR000547. Clathrin_H-chain/VPS_repeat. IPR007810. Pep3_Vps18. [Graphical view]
Pfam	PF00637. Clathrin. 1 hit. PF05131. Pep3_Vps18. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	64286.
SOURCE	Search...

Entry information

Entry name

VPS18_HUMAN

Accession

Primary (citable) accession number: Q9P253
Secondary accession number(s): Q8TCG0, Q96DI3, Q9H268

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	January 25, 2012
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.