Reviewed, UniProtKB/Swiss-Prot Q9P253 (VPS18_HUMAN)

Names and origin

Protein names

Recommended name:
    Vacuolar protein sorting-associated protein 18 homolog
      Short name=hVPS18

Gene names

Name:	VPS18
Synonyms:	KIAA1475

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	973 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Ref.5
Subunit structure	Interacts with HOOK1 By similarity. Large hetero-oligomeric complex together with VPS11, VPS16 and VPS33A. Interacts with STX7.
Subcellular location	Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes. Ref.5
Tissue specificity	Ubiquitous. Expression was highest in heart and low in lung. Ref.5
Sequence similarities	Belongs to the VPS18 family. Contains 1 clathrin repeat. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Lysosome Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endosome organization Inferred from direct assay. Source: UniProtKB lysosome organization Inferred from direct assay. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	HOPS complex Inferred from direct assay. Source: UniProtKB lysosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCNA1	P78396	1	EBI-1053363,EBI-375065

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P253-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P253-2)

The sequence of this isoform differs from the canonical sequence as follows:
     519-580: TKECFRTFLS...AYHCQHEAYE → VRNLTQFHPL...SEEAFSSSCL
     581-973: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 973	973	Vacuolar protein sorting-associated protein 18 homolog		PRO_0000055906
Regions
	Repeat	627 – 785	159	Clathrin
	Zinc finger	853 – 947	95	RING-type
	Coiled coil	454 – 481	28	Potential
	Coiled coil	802 – 848	47	Potential
Amino acid modifications
	Modified residue	362	1	N6-acetyllysine Ref.10
	Modified residue	528	1	Phosphoserine Ref.6
	Modified residue	529	1	Phosphoserine Ref.6
	Modified residue	689	1	Phosphoserine Ref.7 Ref.8
Natural variations
	Alternative sequence	519 – 580	62	TKECF…HEAYE → VRNLTQFHPLPLAPLLSLSF PTHVLFTSREREREHLSSVC SLCGLWNPSSSLSEEAFSSS CL in isoform 2.		VSP_004020
	Alternative sequence	581 – 973	393	Missing in isoform 2.		VSP_004021
	Natural variant	913	1	A → S in a colorectal cancer sample; somatic mutation. Ref.11		VAR_035950

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 19, 2002. Version 2. Checksum: 0008E21D66E8CFEC	FASTA	973	110,186
	10 20 30 40 50 60 MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL 70 80 90 100 110 120 VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV HKMFLDHTGS HLLIALSSTE 130 140 150 160 170 180 VLYVNRNGQK VRPLARWKGQ LVESVGWNKA LGTESSTGPI LVGTAQGHIF EAELSASEGG 190 200 210 220 230 240 LFGPAPDLYF RPLYVLNEEG GPAPVCSLEA ERGPDGRSFV IATTRQRLFQ FIGRAAEGAE 250 260 270 280 290 300 AQGFSGLFAA YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGALDCG 310 320 330 340 350 360 RPDSLLSEER VWEYPEGVGP GASPPLAIVL TQFHFLLLLA DRVEAVCTLT GQVVLRDHFL 370 380 390 400 410 420 EKFGPLKHMV KDSSTGQLWA YTERAVFRYH VQREARDVWR TYLDMNRFDL AKEYCRERPD 430 440 450 460 470 480 CLDTVLAREA DFCFRQRRYL ESARCYALTQ SYFEEIALKF LEARQEEALA EFLQRKLASL 490 500 510 520 530 540 KPAERTQATL LTTWLTELYL SRLGALQGDP EALTLYRETK ECFRTFLSSP RHKEWLFASR 550 560 570 580 590 600 ASIHELLASH GDTEHMVYFA VIMQDYERVV AYHCQHEAYE EALAVLARHR DPQLFYKFSP 610 620 630 640 650 660 ILIRHIPRQL VDAWIEMGSR LDARQLIPAL VNYSQGGEVQ QVSQAIRYME FCVNVLGETE 670 680 690 700 710 720 QAIHNYLLSL YARGRPDSLL AYLEQAGASP HRVHYDLKYA LRLCAEHGHH RACVHVYKVL 730 740 750 760 770 780 ELYEEAVDLA LQVDVDLAKQ CADLPEEDEE LRKKLWLKIA RHVVQEEEDV QTAMACLASC 790 800 810 820 830 840 PLLKIEDVLP FFPDFVTIDH FKEAICSSLK AYNHHIQELQ REMEEATASA QRIRRDLQEL 850 860 870 880 890 900 RGRYGTVEPQ DKCATCDFPL LNRPFYLFLC GHMFHADCLL QAVRPGLPAY KQARLEELQR 910 920 930 940 950 960 KLGAAPPPAK GSARAKEAEG GAATAGPSRE QLKADLDELV AAECVYCGEL MIRSIDRPFI 970 DPQRYEEEQL SWL
	Isoform 2. Checksum: 9FB02B4160595E32	FASTA	580	64,959
	10 20 30 40 50 60 MASILDEYEN SLSRSAVLQP GCPSVGIPHS GYVNAQLEKE VPIFTKQRID FTPSERITSL 70 80 90 100 110 120 VVSSNQLCMS LGKDTLLRID LGKANEPNHV ELGRKDDAKV HKMFLDHTGS HLLIALSSTE 130 140 150 160 170 180 VLYVNRNGQK VRPLARWKGQ LVESVGWNKA LGTESSTGPI LVGTAQGHIF EAELSASEGG 190 200 210 220 230 240 LFGPAPDLYF RPLYVLNEEG GPAPVCSLEA ERGPDGRSFV IATTRQRLFQ FIGRAAEGAE 250 260 270 280 290 300 AQGFSGLFAA YTDHPPPFRE FPSNLGYSEL AFYTPKLRSA PRAFAWMMGD GVLYGALDCG 310 320 330 340 350 360 RPDSLLSEER VWEYPEGVGP GASPPLAIVL TQFHFLLLLA DRVEAVCTLT GQVVLRDHFL 370 380 390 400 410 420 EKFGPLKHMV KDSSTGQLWA YTERAVFRYH VQREARDVWR TYLDMNRFDL AKEYCRERPD 430 440 450 460 470 480 CLDTVLAREA DFCFRQRRYL ESARCYALTQ SYFEEIALKF LEARQEEALA EFLQRKLASL 490 500 510 520 530 540 KPAERTQATL LTTWLTELYL SRLGALQGDP EALTLYREVR NLTQFHPLPL APLLSLSFPT 550 560 570 580 HVLFTSRERE REHLSSVCSL CGLWNPSSSL SEEAFSSSCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of human VPS18, VPS11, VPS16, and VPS33." Huizing M., Didier A., Walenta J., Anikster Y., Gahl W.A., Kraemer H. Gene 264:241-247(2001) [PubMed: 11250079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed: 10819331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Melanoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung.
[5]	"Molecular characterization of mammalian homologues of class c Vps proteins that interact with syntaxin-7." Kim B.Y., Kraemer H., Yamamoto A., Kominami E., Kohsaka S., Akazawa C. J. Biol. Chem. 276:29393-29402(2001) [PubMed: 11382755] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-528 AND SER-529, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-362, MASS SPECTROMETRY.
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-913.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF308802 mRNA. Translation: AAG34679.1. AB040908 mRNA. Translation: BAA95999.1. Different initiation. AL713725 mRNA. Translation: CAD28515.1. BC001513 mRNA. Translation: AAH01513.1.
IPI	IPI00001985. IPI00333553.
RefSeq	NP_065908.1.
UniGene	Hs.23876
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P253. 1 interaction.
STRING	Q9P253.
PTM databases
PhosphoSite	Q9P253.
Proteomic databases
PeptideAtlas	Q9P253.
PRIDE	Q9P253.
Genome annotation databases
Ensembl	ENST00000220509; ENSP00000220509; ENSG00000104142; Homo sapiens. [Genome view]
GeneID	57617.
KEGG	hsa:57617.
UCSC	uc001zne.1. human.
Organism-specific databases
CTD	57617.
GeneCards	GC15P038974.
H-InvDB	HIX0012143.
HGNC	HGNC:15972. VPS18.
MIM	608551. gene.
PharmGKB	PA38069.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG356873.
HOVERGEN	Q9P253.
InParanoid	Q9P253.
OMA	NRPFYLF.
OrthoDB	EOG98GZNG.
Gene expression databases
ArrayExpress	Q9P253.
Bgee	Q9P253.
CleanEx	HS_VPS18.
Genevestigator	Q9P253.
GermOnline	ENSG00000104142. Homo sapiens.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR007810. Pep3_Vps18. [Graphical view]
Pfam	PF05131. Pep3_Vps18. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	64286.
SOURCE	Search...

Entry information

Entry name

VPS18_HUMAN

Accession

Primary (citable) accession number: Q9P253
Secondary accession number(s): Q8TCG0, Q96DI3, Q9H268

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	September 19, 2002
Last modified:	December 15, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.