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Protein

Stromal interaction molecule 2

Gene

STIM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. Functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca2+ influx.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi80 – 9112Add
BLAST

GO - Molecular functioni

  • calcium channel regulator activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • store-operated calcium channel activity Source: UniProtKB

GO - Biological processi

  • activation of store-operated calcium channel activity Source: UniProtKB
  • cellular calcium ion homeostasis Source: UniProtKB
  • positive regulation of calcium ion transport Source: UniProtKB
  • store-operated calcium entry Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

TCDBi1.A.52.1.1. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal interaction molecule 2
Gene namesi
Name:STIM2
Synonyms:KIAA1482
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:19205. STIM2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini15 – 218204ExtracellularSequence analysisAdd
BLAST
Transmembranei219 – 23517HelicalSequence analysisAdd
BLAST
Topological domaini236 – 746511CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi80 – 801D → A: No effect on inhibitory activity; when associated with A-91. 2 Publications
Mutagenesisi91 – 911E → A: No effect on inhibitory activity; when associated with A-80. 1 Publication

Organism-specific databases

PharmGKBiPA134926985.

Polymorphism and mutation databases

BioMutaiSTIM2.
DMDMi17369338.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 14141 PublicationAdd
BLAST
Chaini15 – 746732Stromal interaction molecule 2PRO_0000033328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence analysis
Modified residuei523 – 5231PhosphoserineCombined sources
Modified residuei609 – 6091PhosphoserineCombined sources
Modified residuei621 – 6211PhosphoserineCombined sources
Modified residuei640 – 6401PhosphoserineBy similarity
Modified residuei650 – 6501PhosphoserineBy similarity
Modified residuei661 – 6611PhosphoserineCombined sources
Modified residuei665 – 6651PhosphoserineCombined sources
Modified residuei680 – 6801PhosphoserineCombined sources
Modified residuei697 – 6971PhosphoserineCombined sources

Post-translational modificationi

Glycosylated.1 Publication
Phosphorylated predominantly on Ser residues.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9P246.
MaxQBiQ9P246.
PaxDbiQ9P246.
PeptideAtlasiQ9P246.
PRIDEiQ9P246.

PTM databases

iPTMnetiQ9P246.
PhosphoSiteiQ9P246.

Expressioni

Tissue specificityi

Expressed in all tissues and tumor cell lines examined.1 Publication

Gene expression databases

BgeeiENSG00000109689.
CleanExiHS_STIM2.
ExpressionAtlasiQ9P246. baseline and differential.
GenevisibleiQ9P246. HS.

Organism-specific databases

HPAiCAB037196.
HPA036933.
HPA057511.

Interactioni

Subunit structurei

Oligomer with STIM1. Interacts with ORAI1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STIM1Q135864EBI-448891,EBI-448878

Protein-protein interaction databases

BioGridi121666. 18 interactions.
IntActiQ9P246. 6 interactions.
MINTiMINT-1195661.
STRINGi9606.ENSP00000417569.

Structurei

Secondary structure

1
746
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi63 – 7917Combined sources
Beta strandi80 – 823Combined sources
Helixi89 – 968Combined sources
Turni97 – 1037Combined sources
Helixi106 – 1127Combined sources
Turni113 – 1153Combined sources
Helixi121 – 1299Combined sources
Helixi132 – 1354Combined sources
Helixi138 – 14811Combined sources
Helixi155 – 1628Combined sources
Beta strandi164 – 1663Combined sources
Turni167 – 1704Combined sources
Beta strandi173 – 1753Combined sources
Turni179 – 1824Combined sources
Helixi188 – 20417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L5YNMR-A62-205[»]
ProteinModelPortaliQ9P246.
SMRiQ9P246. Positions 62-205, 251-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 10236EF-handAdd
BLAST
Domaini136 – 20469SAMPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili247 – 394148Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi533 – 55927His/Pro-richAdd
BLAST
Compositional biasi730 – 74617Lys-richAdd
BLAST

Sequence similaritiesi

Contains 1 EF-hand domain.Curated
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
HOGENOMiHOG000261647.
HOVERGENiHBG079219.
InParanoidiQ9P246.
KOiK18196.
PhylomeDBiQ9P246.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P246-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVLGLLVAG AADGCELVPR HLRGRRATGS AATAASSPAA AAGDSPALMT
60 70 80 90 100
DPCMSLSPPC FTEEDRFSLE ALQTIHKQMD DDKDGGIEVE ESDEFIREDM
110 120 130 140 150
KYKDATNKHS HLHREDKHIT IEDLWKRWKT SEVHNWTLED TLQWLIEFVE
160 170 180 190 200
LPQYEKNFRD NNVKGTTLPR IAVHEPSFMI SQLKISDRSH RQKLQLKALD
210 220 230 240 250
VVLFGPLTRP PHNWMKDFIL TVSIVIGVGG CWFAYTQNKT SKEHVAKMMK
260 270 280 290 300
DLESLQTAEQ SLMDLQERLE KAQEENRNVA VEKQNLERKM MDEINYAKEE
310 320 330 340 350
ACRLRELREG AECELSRRQY AEQELEQVRM ALKKAEKEFE LRSSWSVPDA
360 370 380 390 400
LQKWLQLTHE VEVQYYNIKR QNAEMQLAIA KDEAEKIKKK RSTVFGTLHV
410 420 430 440 450
AHSSSLDEVD HKILEAKKAL SELTTCLRER LFRWQQIEKI CGFQIAHNSG
460 470 480 490 500
LPSLTSSLYS DHSWVVMPRV SIPPYPIAGG VDDLDEDTPP IVSQFPGTMA
510 520 530 540 550
KPPGSLARSS SLCRSRRSIV PSSPQPQRAQ LAPHAPHPSH PRHPHHPQHT
560 570 580 590 600
PHSLPSPDPD ILSVSSCPAL YRNEEEEEAI YFSAEKQWEV PDTASECDSL
610 620 630 640 650
NSSIGRKQSP PLSLEIYQTL SPRKISRDEV SLEDSSRGDS PVTVDVSWGS
660 670 680 690 700
PDCVGLTETK SMIFSPASKV YNGILEKSCS MNQLSSGIPV PKPRHTSCSS
710 720 730 740
AGNDSKPVQE APSVARISSI PHDLCHNGEK SKKPSKIKSL FKKKSK
Length:746
Mass (Da):83,971
Last modified:October 1, 2001 - v2
Checksum:iB83855557F126254
GO
Isoform 2 (identifier: Q9P246-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNAAGIRAPE...LLRRRRWAAL
     383-383: E → EVAASYLIQ

Note: No experimental confirmation available.
Show »
Length:841
Mass (Da):93,957
Checksum:iCD6ACEB84DE56442
GO
Isoform 3 (identifier: Q9P246-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     588-599: WEVPDTASECDS → CIHLGLGACKSE
     600-746: Missing.

Note: No experimental confirmation available.
Show »
Length:599
Mass (Da):68,026
Checksum:iCCD0B00D7B86E051
GO

Sequence cautioni

The sequence BAA96006 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.Curated
The sequence BAB14545 differs from that shown.Probable cloning artifact.Curated
The sequence BAB14545 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB66512 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → P in CAB66512 (PubMed:11230166).Curated
Sequence conflicti308 – 3081R → G in AAH57231 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MNAAGIRAPEAAGADGTRLA PGGSPCLRRRGRPEESPAAV VAPRGAGELQAAGAPLRFYP ASPRRLHRASTPGPAWGWLL RRRRWAAL in isoform 2. 1 PublicationVSP_057171
Alternative sequencei383 – 3831E → EVAASYLIQ in isoform 2. 1 PublicationVSP_057172
Alternative sequencei588 – 59912WEVPD…SECDS → CIHLGLGACKSE in isoform 3. 1 PublicationVSP_057173Add
BLAST
Alternative sequencei600 – 746147Missing in isoform 3. 1 PublicationVSP_057174Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF328905 mRNA. Translation: AAK82337.1.
AB040915 mRNA. Translation: BAA96006.1. Sequence problems.
AL136577 mRNA. Translation: CAB66512.2. Sequence problems.
BC015659 mRNA. Translation: AAH15659.2.
BC057231 mRNA. Translation: AAH57231.1.
BC136449 mRNA. Translation: AAI36450.1.
BC146661 mRNA. Translation: AAI46662.1.
BC152554 mRNA. Translation: AAI52555.1.
BC171766 mRNA. Translation: AAI71766.1.
AK023369 mRNA. Translation: BAB14545.1. Sequence problems.
CCDSiCCDS3440.2. [Q9P246-1]
CCDS54752.1. [Q9P246-3]
RefSeqiNP_001162588.1. NM_001169117.1. [Q9P246-3]
NP_001162589.1. NM_001169118.1.
NP_065911.3. NM_020860.3. [Q9P246-1]
UniGeneiHs.744950.

Genome annotation databases

EnsembliENST00000412829; ENSP00000404812; ENSG00000109689.
ENST00000467087; ENSP00000419073; ENSG00000109689.
GeneIDi57620.
KEGGihsa:57620.
UCSCiuc003gsg.6. human. [Q9P246-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF328905 mRNA. Translation: AAK82337.1.
AB040915 mRNA. Translation: BAA96006.1. Sequence problems.
AL136577 mRNA. Translation: CAB66512.2. Sequence problems.
BC015659 mRNA. Translation: AAH15659.2.
BC057231 mRNA. Translation: AAH57231.1.
BC136449 mRNA. Translation: AAI36450.1.
BC146661 mRNA. Translation: AAI46662.1.
BC152554 mRNA. Translation: AAI52555.1.
BC171766 mRNA. Translation: AAI71766.1.
AK023369 mRNA. Translation: BAB14545.1. Sequence problems.
CCDSiCCDS3440.2. [Q9P246-1]
CCDS54752.1. [Q9P246-3]
RefSeqiNP_001162588.1. NM_001169117.1. [Q9P246-3]
NP_001162589.1. NM_001169118.1.
NP_065911.3. NM_020860.3. [Q9P246-1]
UniGeneiHs.744950.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L5YNMR-A62-205[»]
ProteinModelPortaliQ9P246.
SMRiQ9P246. Positions 62-205, 251-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121666. 18 interactions.
IntActiQ9P246. 6 interactions.
MINTiMINT-1195661.
STRINGi9606.ENSP00000417569.

Protein family/group databases

TCDBi1.A.52.1.1. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

PTM databases

iPTMnetiQ9P246.
PhosphoSiteiQ9P246.

Polymorphism and mutation databases

BioMutaiSTIM2.
DMDMi17369338.

Proteomic databases

EPDiQ9P246.
MaxQBiQ9P246.
PaxDbiQ9P246.
PeptideAtlasiQ9P246.
PRIDEiQ9P246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000412829; ENSP00000404812; ENSG00000109689.
ENST00000467087; ENSP00000419073; ENSG00000109689.
GeneIDi57620.
KEGGihsa:57620.
UCSCiuc003gsg.6. human. [Q9P246-1]

Organism-specific databases

CTDi57620.
GeneCardsiSTIM2.
HGNCiHGNC:19205. STIM2.
HPAiCAB037196.
HPA036933.
HPA057511.
MIMi610841. gene.
neXtProtiNX_Q9P246.
PharmGKBiPA134926985.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
HOGENOMiHOG000261647.
HOVERGENiHBG079219.
InParanoidiQ9P246.
KOiK18196.
PhylomeDBiQ9P246.

Miscellaneous databases

ChiTaRSiSTIM2. human.
GeneWikiiSTIM2.
GenomeRNAii57620.
PROiQ9P246.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000109689.
CleanExiHS_STIM2.
ExpressionAtlasiQ9P246. baseline and differential.
GenevisibleiQ9P246. HS.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTIM2_HUMAN
AccessioniPrimary (citable) accession number: Q9P246
Secondary accession number(s): A6H8L7
, B7ZVY0, Q96BF1, Q9BQH2, Q9H8R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: October 1, 2001
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.