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Protein

Stromal interaction molecule 2

Gene

STIM2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. Functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca2+ influx.7 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi80 – 91Add BLAST12

GO - Molecular functioni

  • calcium channel regulator activity Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • store-operated calcium channel activity Source: UniProtKB

GO - Biological processi

  • activation of store-operated calcium channel activity Source: UniProtKB
  • cellular calcium ion homeostasis Source: UniProtKB
  • positive regulation of calcium ion transport Source: UniProtKB
  • store-operated calcium entry Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000109689-MONOMER.

Protein family/group databases

TCDBi1.A.52.1.1. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal interaction molecule 2
Gene namesi
Name:STIM2
Synonyms:KIAA1482
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:19205. STIM2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini15 – 218ExtracellularSequence analysisAdd BLAST204
Transmembranei219 – 235HelicalSequence analysisAdd BLAST17
Topological domaini236 – 746CytoplasmicSequence analysisAdd BLAST511

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi80D → A: No effect on inhibitory activity; when associated with A-91. 2 Publications1
Mutagenesisi91E → A: No effect on inhibitory activity; when associated with A-80. 1 Publication1

Organism-specific databases

DisGeNETi57620.
PharmGKBiPA134926985.

Polymorphism and mutation databases

BioMutaiSTIM2.
DMDMi17369338.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 141 PublicationAdd BLAST14
ChainiPRO_000003332815 – 746Stromal interaction molecule 2Add BLAST732

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Modified residuei523PhosphoserineCombined sources1
Modified residuei609PhosphoserineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei640PhosphoserineBy similarity1
Modified residuei650PhosphoserineBy similarity1
Modified residuei661PhosphoserineCombined sources1
Modified residuei665PhosphoserineCombined sources1
Modified residuei680PhosphoserineCombined sources1
Modified residuei697PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.1 Publication
Phosphorylated predominantly on Ser residues.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9P246.
MaxQBiQ9P246.
PaxDbiQ9P246.
PeptideAtlasiQ9P246.
PRIDEiQ9P246.

PTM databases

iPTMnetiQ9P246.
PhosphoSitePlusiQ9P246.

Expressioni

Tissue specificityi

Expressed in all tissues and tumor cell lines examined.1 Publication

Gene expression databases

BgeeiENSG00000109689.
CleanExiHS_STIM2.
ExpressionAtlasiQ9P246. baseline and differential.
GenevisibleiQ9P246. HS.

Organism-specific databases

HPAiCAB037196.
HPA036933.
HPA057511.

Interactioni

Subunit structurei

Oligomer with STIM1. Interacts with ORAI1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STIM1Q135864EBI-448891,EBI-448878

Protein-protein interaction databases

BioGridi121666. 18 interactors.
IntActiQ9P246. 6 interactors.
MINTiMINT-1195661.
STRINGi9606.ENSP00000417569.

Structurei

Secondary structure

1746
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi63 – 79Combined sources17
Beta strandi80 – 82Combined sources3
Helixi89 – 96Combined sources8
Turni97 – 103Combined sources7
Helixi106 – 112Combined sources7
Turni113 – 115Combined sources3
Helixi121 – 129Combined sources9
Helixi132 – 135Combined sources4
Helixi138 – 148Combined sources11
Helixi155 – 162Combined sources8
Beta strandi164 – 166Combined sources3
Turni167 – 170Combined sources4
Beta strandi173 – 175Combined sources3
Turni179 – 182Combined sources4
Helixi188 – 204Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L5YNMR-A62-205[»]
ProteinModelPortaliQ9P246.
SMRiQ9P246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 102EF-handAdd BLAST36
Domaini136 – 204SAMPROSITE-ProRule annotationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili247 – 394Sequence analysisAdd BLAST148

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi533 – 559His/Pro-richAdd BLAST27
Compositional biasi730 – 746Lys-richAdd BLAST17

Sequence similaritiesi

Contains 1 EF-hand domain.Curated
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
HOGENOMiHOG000261647.
HOVERGENiHBG079219.
InParanoidiQ9P246.
KOiK18196.
PhylomeDBiQ9P246.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P246-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVLGLLVAG AADGCELVPR HLRGRRATGS AATAASSPAA AAGDSPALMT
60 70 80 90 100
DPCMSLSPPC FTEEDRFSLE ALQTIHKQMD DDKDGGIEVE ESDEFIREDM
110 120 130 140 150
KYKDATNKHS HLHREDKHIT IEDLWKRWKT SEVHNWTLED TLQWLIEFVE
160 170 180 190 200
LPQYEKNFRD NNVKGTTLPR IAVHEPSFMI SQLKISDRSH RQKLQLKALD
210 220 230 240 250
VVLFGPLTRP PHNWMKDFIL TVSIVIGVGG CWFAYTQNKT SKEHVAKMMK
260 270 280 290 300
DLESLQTAEQ SLMDLQERLE KAQEENRNVA VEKQNLERKM MDEINYAKEE
310 320 330 340 350
ACRLRELREG AECELSRRQY AEQELEQVRM ALKKAEKEFE LRSSWSVPDA
360 370 380 390 400
LQKWLQLTHE VEVQYYNIKR QNAEMQLAIA KDEAEKIKKK RSTVFGTLHV
410 420 430 440 450
AHSSSLDEVD HKILEAKKAL SELTTCLRER LFRWQQIEKI CGFQIAHNSG
460 470 480 490 500
LPSLTSSLYS DHSWVVMPRV SIPPYPIAGG VDDLDEDTPP IVSQFPGTMA
510 520 530 540 550
KPPGSLARSS SLCRSRRSIV PSSPQPQRAQ LAPHAPHPSH PRHPHHPQHT
560 570 580 590 600
PHSLPSPDPD ILSVSSCPAL YRNEEEEEAI YFSAEKQWEV PDTASECDSL
610 620 630 640 650
NSSIGRKQSP PLSLEIYQTL SPRKISRDEV SLEDSSRGDS PVTVDVSWGS
660 670 680 690 700
PDCVGLTETK SMIFSPASKV YNGILEKSCS MNQLSSGIPV PKPRHTSCSS
710 720 730 740
AGNDSKPVQE APSVARISSI PHDLCHNGEK SKKPSKIKSL FKKKSK
Length:746
Mass (Da):83,971
Last modified:October 1, 2001 - v2
Checksum:iB83855557F126254
GO
Isoform 2 (identifier: Q9P246-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MNAAGIRAPE...LLRRRRWAAL
     383-383: E → EVAASYLIQ

Note: No experimental confirmation available.
Show »
Length:841
Mass (Da):93,957
Checksum:iCD6ACEB84DE56442
GO
Isoform 3 (identifier: Q9P246-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     588-599: WEVPDTASECDS → CIHLGLGACKSE
     600-746: Missing.

Note: No experimental confirmation available.
Show »
Length:599
Mass (Da):68,026
Checksum:iCCD0B00D7B86E051
GO

Sequence cautioni

The sequence BAA96006 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.Curated
The sequence BAB14545 differs from that shown. Probable cloning artifact.Curated
The sequence BAB14545 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAB66512 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9A → P in CAB66512 (PubMed:11230166).Curated1
Sequence conflicti308R → G in AAH57231 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0571711M → MNAAGIRAPEAAGADGTRLA PGGSPCLRRRGRPEESPAAV VAPRGAGELQAAGAPLRFYP ASPRRLHRASTPGPAWGWLL RRRRWAAL in isoform 2. 1 Publication1
Alternative sequenceiVSP_057172383E → EVAASYLIQ in isoform 2. 1 Publication1
Alternative sequenceiVSP_057173588 – 599WEVPD…SECDS → CIHLGLGACKSE in isoform 3. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_057174600 – 746Missing in isoform 3. 1 PublicationAdd BLAST147

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF328905 mRNA. Translation: AAK82337.1.
AB040915 mRNA. Translation: BAA96006.1. Sequence problems.
AL136577 mRNA. Translation: CAB66512.2. Sequence problems.
BC015659 mRNA. Translation: AAH15659.2.
BC057231 mRNA. Translation: AAH57231.1.
BC136449 mRNA. Translation: AAI36450.1.
BC146661 mRNA. Translation: AAI46662.1.
BC152554 mRNA. Translation: AAI52555.1.
BC171766 mRNA. Translation: AAI71766.1.
AK023369 mRNA. Translation: BAB14545.1. Sequence problems.
CCDSiCCDS3440.2. [Q9P246-1]
CCDS54752.1. [Q9P246-3]
RefSeqiNP_001162588.1. NM_001169117.1. [Q9P246-3]
NP_001162589.1. NM_001169118.1.
NP_065911.3. NM_020860.3. [Q9P246-1]
UniGeneiHs.744950.

Genome annotation databases

EnsembliENST00000412829; ENSP00000404812; ENSG00000109689.
ENST00000467087; ENSP00000419073; ENSG00000109689.
GeneIDi57620.
KEGGihsa:57620.
UCSCiuc003gsg.6. human. [Q9P246-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF328905 mRNA. Translation: AAK82337.1.
AB040915 mRNA. Translation: BAA96006.1. Sequence problems.
AL136577 mRNA. Translation: CAB66512.2. Sequence problems.
BC015659 mRNA. Translation: AAH15659.2.
BC057231 mRNA. Translation: AAH57231.1.
BC136449 mRNA. Translation: AAI36450.1.
BC146661 mRNA. Translation: AAI46662.1.
BC152554 mRNA. Translation: AAI52555.1.
BC171766 mRNA. Translation: AAI71766.1.
AK023369 mRNA. Translation: BAB14545.1. Sequence problems.
CCDSiCCDS3440.2. [Q9P246-1]
CCDS54752.1. [Q9P246-3]
RefSeqiNP_001162588.1. NM_001169117.1. [Q9P246-3]
NP_001162589.1. NM_001169118.1.
NP_065911.3. NM_020860.3. [Q9P246-1]
UniGeneiHs.744950.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2L5YNMR-A62-205[»]
ProteinModelPortaliQ9P246.
SMRiQ9P246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121666. 18 interactors.
IntActiQ9P246. 6 interactors.
MINTiMINT-1195661.
STRINGi9606.ENSP00000417569.

Protein family/group databases

TCDBi1.A.52.1.1. the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.

PTM databases

iPTMnetiQ9P246.
PhosphoSitePlusiQ9P246.

Polymorphism and mutation databases

BioMutaiSTIM2.
DMDMi17369338.

Proteomic databases

EPDiQ9P246.
MaxQBiQ9P246.
PaxDbiQ9P246.
PeptideAtlasiQ9P246.
PRIDEiQ9P246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000412829; ENSP00000404812; ENSG00000109689.
ENST00000467087; ENSP00000419073; ENSG00000109689.
GeneIDi57620.
KEGGihsa:57620.
UCSCiuc003gsg.6. human. [Q9P246-1]

Organism-specific databases

CTDi57620.
DisGeNETi57620.
GeneCardsiSTIM2.
HGNCiHGNC:19205. STIM2.
HPAiCAB037196.
HPA036933.
HPA057511.
MIMi610841. gene.
neXtProtiNX_Q9P246.
PharmGKBiPA134926985.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
HOGENOMiHOG000261647.
HOVERGENiHBG079219.
InParanoidiQ9P246.
KOiK18196.
PhylomeDBiQ9P246.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000109689-MONOMER.

Miscellaneous databases

ChiTaRSiSTIM2. human.
GeneWikiiSTIM2.
GenomeRNAii57620.
PROiQ9P246.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000109689.
CleanExiHS_STIM2.
ExpressionAtlasiQ9P246. baseline and differential.
GenevisibleiQ9P246. HS.

Family and domain databases

CDDicd11722. SOAR. 1 hit.
Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTIM2_HUMAN
AccessioniPrimary (citable) accession number: Q9P246
Secondary accession number(s): A6H8L7
, B7ZVY0, Q96BF1, Q9BQH2, Q9H8R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: October 1, 2001
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.