Reviewed, UniProtKB/Swiss-Prot Q9P232 (CNTN3_HUMAN)

Names and origin

Protein names

Recommended name:
    Contactin-3
Alternative name(s):
    Brain-derived immunoglobulin superfamily protein 1
      Short name=BIG-1
    Plasmacytoma-associated neuronal glycoprotein

Gene names

Name:	CNTN3
Synonyms:	KIAA1496, PANG

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1028 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Tissue specificity	In brain, it is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Ref.4
Sequence similarities	Belongs to the immunoglobulin superfamily. Contactin family. Contains 4 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Repeat Signal
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APLP1	P51693	1	EBI-2028361,EBI-74648
APP	P05067-4	1	EBI-2028361,EBI-302641
App	P12023-2	1	EBI-2028361,EBI-286828	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Potential

Chain

20 – 1002

983

Contactin-3

PRO_0000014705

Propeptide

1003 – 1028

26

Removed in mature form Potential

PRO_0000014706

Regions

Domain

26 – 117

92

Ig-like C2-type 1

Domain

122 – 208

87

Ig-like C2-type 2

Domain

227 – 313

87

Ig-like C2-type 3

Domain

318 – 402

85

Ig-like C2-type 4

Domain

408 – 497

90

Ig-like C2-type 5

Domain

499 – 593

95

Ig-like C2-type 6

Domain

598 – 693

96

Fibronectin type-III 1

Domain

700 – 798

99

Fibronectin type-III 2

Domain

802 – 898

97

Fibronectin type-III 3

Domain

903 – 992

90

Fibronectin type-III 4

Amino acid modifications

Lipidation

1002

1

GPI-anchor amidated serine Potential

Glycosylation

65

1

N-linked (GlcNAc...) Potential

Glycosylation

193

1

N-linked (GlcNAc...) Potential

Glycosylation

375

1

N-linked (GlcNAc...) Potential

Glycosylation

468

1

N-linked (GlcNAc...) Potential

Glycosylation

489

1

N-linked (GlcNAc...) Ref.5

Glycosylation

765

1

N-linked (GlcNAc...) Potential

Glycosylation

860

1

N-linked (GlcNAc...) Ref.5

Glycosylation

895

1

N-linked (GlcNAc...) Potential

Glycosylation

913

1

N-linked (GlcNAc...) Potential

Glycosylation

931

1

N-linked (GlcNAc...) Ref.5

Glycosylation

956

1

N-linked (GlcNAc...) Potential

Disulfide bond

50 ↔ 100

By similarity

Disulfide bond

144 ↔ 196

By similarity

Disulfide bond

249 ↔ 297

By similarity

Disulfide bond

339 ↔ 386

By similarity

Disulfide bond

431 ↔ 479

By similarity

Disulfide bond

521 ↔ 577

By similarity

Natural variations

Natural variant

630

1

S → N: dbSNP rs626578.

VAR_019906

Natural variant

708

1

N → S: dbSNP rs626578.

VAR_056042

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

1028

Helix Strand Turn

Details...

Beta strand

918 – 922

5

Beta strand

930 – 932

3

Beta strand

935 – 946

12

Beta strand

951 – 963

13

Beta strand

970 – 980

11

Beta strand

989 – 991

3

Sequences

	Sequence		Length	Mass (Da)
	Q9P232-1 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 577DB096828CF844	FASTA	1,028	112,839
	10 20 30 40 50 60 MMLSWKQLIL LSFIGCLGGE LLLQGPVFIK EPSNSIFPVG SEDKKITLHC EARGNPSPHY 70 80 90 100 110 120 RWQLNGSDID MSMEHRYKLN GGNLVVINPN RNWDTGTYQC FATNSLGTIV SREAKLQFAY 130 140 150 160 170 180 LENFKTKMRS TVSVREGQGV VLLCGPPPHS GELSYAWIFN EYPSFVEEDS RRFVSQETGH 190 200 210 220 230 240 LYISKVEPSD VGNYTCVVTS MVTNARVLGS PTPLVLRSDG VMGEYEPKIE VQFPETLPAA 250 260 270 280 290 300 KGSTVKLECF ALGNPIPQIN WRRSDGLPFS SKIKLRKFSG VLEIPNFQQE DAGSYECIAE 310 320 330 340 350 360 NSRGKNVARG RLTYYAKPHW VQLIKDVEIA VEDSLYWECR ASGKPKPSYR WLKNGAALVL 370 380 390 400 410 420 EERTQIENGA LTISNLSVTD SGMFQCIAEN KHGLVYSSAE LKVVASAPDF SKNPMKKLVQ 430 440 450 460 470 480 VQVGSLVSLD CKPRASPRAL SSWKKGDVSV QEHERISLLN DGGLKIANVT KADAGTYTCM 490 500 510 520 530 540 AENQFGKANG TTHLVVTEPT RITLAPSNMD VSVGESVILP CQVQHDPLLD IIFTWYFNGA 550 560 570 580 590 600 LADFKKDGSH FEKVGGSSSG DLMIRNIQLK HSGKYVCMVQ TGVDSVSSAA DLIVRGSPGP 610 620 630 640 650 660 PENVKVDEIT DTTAQLSWKE GKDNHSPVIS YSIQARTPFS VGWQTVTTVP EVIDGKTHTA 670 680 690 700 710 720 TVVELNPWVE YEFRVVASNK IGGGEPSLPS EKVRTEEAVP EVPPSEVNGG GGSRSELVIT 730 740 750 760 770 780 WDPVPEELQN GEGFGYVVAF RPLGVTTWIQ TVVTSPDTPR YVFRNESIVP YSPYEVKVGV 790 800 810 820 830 840 YNNKGEGPFS PVTTVFSAEE EPTVAPSQVS ANSLSSSEIE VSWNTIPWKL SNGHLLGYEV 850 860 870 880 890 900 RYWNGGGKEE SSSKMKVAGN ETSARLRGLK SNLAYYTAVR AYNSAGAGPF SATVNVTTKK 910 920 930 940 950 960 TPPSQPPGNV VWNATDTKVL LNWEQVKAME NESEVTGYKV FYRTSSQNNV QVLNTNKTSA 970 980 990 1000 1010 1020 ELVLPIKEDY IIEVKATTDG GDGTSSEQIR IPRITSMDAR GSTSAISNVH PMSSYMPIVL FLIVYVLW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed: 16641997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed: 10819331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 109-1028. Tissue: Brain.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 984-1028. Tissue: Brain.
[4]	"Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members." Kamei Y., Takeda Y., Teramoto K., Tsutsumi O., Taketani Y., Watanabe K. Genomics 69:113-119(2000) [PubMed: 11013081] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-489; ASN-860 AND ASN-931, MASS SPECTROMETRY. Tissue: Plasma.
[6]	"Solution structure of the fourth FN3 domain of KIAA1496 protein." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 893-996.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AC016950 Genomic DNA. No translation available.
AC024222 Genomic DNA. No translation available.
AC128653 Genomic DNA. No translation available.
AC128656 Genomic DNA. No translation available.
AB040929 mRNA. Translation: BAA96020.1.
AL512746 mRNA. Translation: CAC21671.1.

IPI

IPI00292791.

RefSeq

NP_065923.1.

UniGene

Hs.12723

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WJ3	NMR	-	A	893-996	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9P232. 3 interactions.

STRING

Q9P232.

PTM databases

PhosphoSite

Q9P232.

Proteomic databases

PRIDE

Q9P232.

Genome annotation databases

Ensembl

ENST00000263665; ENSP00000263665; ENSG00000113805; Homo sapiens. [Genome view]

GeneID

5067.

KEGG

hsa:5067.

UCSC

uc003dpm.1. human.

Organism-specific databases

CTD

5067.

GeneCards

GC03M074394.

H-InvDB

HIX0003468.

HGNC

HGNC:2173. CNTN3.

HPA

HPA003341.

MIM

601325. gene.

PharmGKB

PA26687.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q9P232.

HOVERGEN

Q9P232.

Gene expression databases

ArrayExpress

Q9P232.

Bgee

Q9P232.

CleanEx

HS_CNTN3.

Genevestigator

Q9P232.

GermOnline

ENSG00000113805. Homo sapiens.

Family and domain databases

InterPro

IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR013151. Ig.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]

Gene3D

G3DSA:2.60.40.30. FN_III-like. 3 hits.
G3DSA:2.60.40.10. Ig-like_fold. 5 hits.

Pfam

PF00041. fn3. 4 hits.
PF07679. I-set. 3 hits.
PF00047. ig. 2 hits.
PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00060. FN3. 4 hits.
SM00409. IG. 2 hits.
SM00408. IGc2. 4 hits.
[Graphical view]

PROSITE

PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 6 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

19522.

SOURCE

Search...

Entry information

Entry name

CNTN3_HUMAN

Accession

Primary (citable) accession number: Q9P232
Secondary accession number(s): Q9H039

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	October 13, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.