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Protein

Zinc finger and BTB domain-containing protein 4

Gene

ZBTB4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional repressor with bimodal DNA-binding specificity. Represses transcription in a methyl-CpG-dependent manner. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Can also bind specifically to a single methyl-CpG pair and can bind hemimethylated DNA but with a lower affinity compared to methylated DNA (PubMed:16354688). Plays a role in postnatal myogenesis, may be involved in the regulation of satellite cells self-renewal (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri234 – 256C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri309 – 331C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri337 – 359C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri365 – 388C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri726 – 748C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri765 – 787C2H2-type 6PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • methyl-CpG binding Source: UniProtKB
  • methyl-CpNpG binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: UniProtKB
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: NTNU_SB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger and BTB domain-containing protein 4
Alternative name(s):
KAISO-like zinc finger protein 1
Short name:
KAISO-L1
Gene namesi
Name:ZBTB4
Synonyms:KIAA1538
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000174282.11
HGNCiHGNC:23847 ZBTB4
MIMi612308 gene
neXtProtiNX_Q9P1Z0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi795T → A: Impaired HIPK2-mediated phosphorylation; when associated with A-797 and A-983. 1 Publication1
Mutagenesisi797T → A: Impaired HIPK2-mediated phosphorylation; when associated with A-795 and A-983. 1 Publication1
Mutagenesisi983T → A: Impaired HIPK2-mediated phosphorylation; when associated with A-795 and A-797. 1 Publication1

Organism-specific databases

DisGeNETi57659
OpenTargetsiENSG00000174282
PharmGKBiPA134959224

Polymorphism and mutation databases

BioMutaiZBTB4
DMDMi46577564

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000477121 – 1013Zinc finger and BTB domain-containing protein 4Add BLAST1013

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki40Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei391PhosphoserineCombined sources1
Cross-linki573Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki615Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei795Phosphothreonine; by HIPK21 Publication1
Modified residuei797Phosphothreonine; by HIPK21 Publication1
Modified residuei983Phosphothreonine; by HIPK21 Publication1

Post-translational modificationi

Phosphorylated by HIPK2. This phosphorylation reduces stability and triggers ZBTB4 protein degradation in response to DNA damage.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9P1Z0
MaxQBiQ9P1Z0
PaxDbiQ9P1Z0
PeptideAtlasiQ9P1Z0
PRIDEiQ9P1Z0

PTM databases

CarbonylDBiQ9P1Z0
iPTMnetiQ9P1Z0
PhosphoSitePlusiQ9P1Z0

Expressioni

Gene expression databases

BgeeiENSG00000174282
CleanExiHS_ZBTB4
GenevisibleiQ9P1Z0 HS

Organism-specific databases

HPAiCAB008980
HPA064763

Interactioni

Subunit structurei

Interacts with HIPK2. Interacts with CBFA2T3. Interacts with ZBTB38.3 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi121693, 7 interactors
IntActiQ9P1Z0, 15 interactors
STRINGi9606.ENSP00000307858

Structurei

3D structure databases

ProteinModelPortaliQ9P1Z0
SMRiQ9P1Z0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 152BTBPROSITE-ProRule annotationAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni186 – 348Interaction with CBFA2T31 PublicationAdd BLAST163

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili627 – 663Sequence analysisAdd BLAST37

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi435 – 536Pro-richAdd BLAST102
Compositional biasi610 – 654Glu-richAdd BLAST45

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri234 – 256C2H2-type 1; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri309 – 331C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri337 – 359C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri365 – 388C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri726 – 748C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri765 – 787C2H2-type 6PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00810000125416
HOGENOMiHOG000065759
HOVERGENiHBG055508
InParanoidiQ9P1Z0
KOiK10491
OMAiLAAYPYN
OrthoDBiEOG091G01R6
PhylomeDBiQ9P1Z0
TreeFamiTF333100

Family and domain databases

InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR011333 SKP1/BTB/POZ_sf
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PfamiView protein in Pfam
PF00651 BTB, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00355 ZnF_C2H2, 6 hits
SUPFAMiSSF54695 SSF54695, 2 hits
SSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 5 hits
PS50157 ZINC_FINGER_C2H2_2, 6 hits

Sequencei

Sequence statusi: Complete.

Q9P1Z0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPPAEVTDP SHAPAVLRQL NEQRLRGLFC DVTLIAGDTK FPAHRSVLAA
60 70 80 90 100
SSPFFREALL TSAPLPLPPA TGGAAPNPAT TTAASSSSSS SSSSSSSSSS
110 120 130 140 150
ASSSSSSSSS SPPPASPPAS SPPRVLELPG VPAAAFSDVL NFIYSARLAL
160 170 180 190 200
PGGGGDGAAV AEIGALGRRL GISRLQGLGE GGDAWVPPTP APMATSQPEE
210 220 230 240 250
DSFGPGPRPA GEWEGDRAEA QAPDLQCSLP RRPLPCPQCG KSFIHPKRLQ
260 270 280 290 300
THEAQCRRGA STRGSTGLGA GGAGPGGPAG VDASALPPPV GFRGGPEHVV
310 320 330 340 350
KVVGGHVLYV CAACERSYVT LSSLKRHSNV HSWRRKYPCR YCEKVFALAE
360 370 380 390 400
YRTKHEVWHT GERRYQCIFC WETFVTYYNL KTHQRAFHGI SPGLLASEKT
410 420 430 440 450
PNGGYKPKLN TLKLYRLLPM RAAKRPYKTY SQGAPEAPLS PTLNTPAPVA
460 470 480 490 500
MPASPPPGPP PAPEPGPPPS VITFAHPAPS VIVHGGSSSG GGGSGTASTG
510 520 530 540 550
GSQAASVITY TAPPRPPKKR EYPPPPPEPA ATPTSPATAV SPATAAGPAM
560 570 580 590 600
ATTTEEAKGR NPRAGRTLTY TAKPVGGIGG GGGPPTGAGR GPSQLQAPPP
610 620 630 640 650
LCQITVRIGE EAIVKRRISE TDLRPGELSG EEMEESEEDE EEEDEEEEEE
660 670 680 690 700
DEEESKAGGE DQLWRPYYSY KPKRKAGAAG GASVGGSGLP RGRRPPRWRQ
710 720 730 740 750
KLERRSWEET PAAESPAGRA RTERRHRCGD CAQTFTTLRK LRKHQEAHGG
760 770 780 790 800
GSHSSRAGRR PSTRFTCPHC AKVCKTAAAL SRHGQRHAAE RPGGTPTPVI
810 820 830 840 850
AYSKGSAGTR PGDVKEEAPQ EMQVSSSSGE AGGGSTAAEE ASETASLQDP
860 870 880 890 900
IISGGEEPPV VASGGSYVYP PVQEFPLALI GGGREPGGGR GKSGSEGPVG
910 920 930 940 950
AGEGDRMEGI GAAKVTFYPE PYPLVYGPQL LAAYPYNFSN LAALPVALNM
960 970 980 990 1000
VLPDEKGAGA LPFLPGVFGY AVNPQAAPPA PPTPPPPTLP PPIPPKGEGE
1010
RAGVERTQKG DVG
Length:1,013
Mass (Da):105,114
Last modified:April 26, 2004 - v3
Checksum:iCA4AB6A5230C2F52
GO

Sequence cautioni

The sequence BAA96062 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188P → S in AAH43352 (PubMed:15489334).Curated1
Sequence conflicti674R → C in AAH43352 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052913539A → V. Corresponds to variant dbSNP:rs35231078Ensembl.1
Natural variantiVAR_018383550M → I. Corresponds to variant dbSNP:rs871990Ensembl.1
Natural variantiVAR_052914561N → S. Corresponds to variant dbSNP:rs34914463Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302699 mRNA Translation: AAP59447.1
AB040971 mRNA Translation: BAA96062.2 Different initiation.
AK122971 mRNA Translation: BAG53828.1
CH471108 Genomic DNA Translation: EAW90185.1
BC033259 mRNA Translation: AAH33259.1
BC043352 mRNA Translation: AAH43352.1
CCDSiCCDS11107.1
RefSeqiNP_001122305.1, NM_001128833.1
NP_065950.2, NM_020899.3
XP_006721626.1, XM_006721563.3
XP_006721627.1, XM_006721564.2
XP_011522274.1, XM_011523972.2
UniGeneiHs.35096

Genome annotation databases

EnsembliENST00000311403; ENSP00000307858; ENSG00000174282
ENST00000380599; ENSP00000369973; ENSG00000174282
ENST00000639670; ENSP00000492232; ENSG00000283868
ENST00000639962; ENSP00000492616; ENSG00000283868
GeneIDi57659
KEGGihsa:57659
UCSCiuc002ghc.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiZBTB4_HUMAN
AccessioniPrimary (citable) accession number: Q9P1Z0
Secondary accession number(s): B3KVL6
, Q7Z697, Q86XJ4, Q8N4V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: May 23, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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