Q9P1Z0 (ZBTB4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger and BTB domain-containing protein 4

Alternative name(s):
KAISO-like zinc finger protein 1
Short name=KAISO-L1

Gene names

Name:	ZBTB4
Synonyms:	KIAA1538

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1013 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subunit structure	Interacts with HIPK2. Ref.7
Subcellular location	Nucleus Potential.
Post-translational modification	Phosphorylated by HIPK2. This phosphorylation reduces stability and triggers ZBTB4 protein degradation in response to DNA damage. Ref.7
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 6 C2H2-type zinc fingers.
Sequence caution	The sequence BAA96062.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1013	1013	Zinc finger and BTB domain-containing protein 4		PRO_0000047712
Regions
	Domain	30 – 152	123	BTB
	Zinc finger	234 – 256	23	C2H2-type 1; atypical
	Zinc finger	309 – 331	23	C2H2-type 2
	Zinc finger	337 – 359	23	C2H2-type 3
	Zinc finger	365 – 388	24	C2H2-type 4
	Zinc finger	726 – 748	23	C2H2-type 5
	Zinc finger	765 – 787	23	C2H2-type 6
	Coiled coil	627 – 663	37	Potential
	Compositional bias	435 – 536	102	Pro-rich
	Compositional bias	610 – 654	45	Glu-rich
Amino acid modifications
	Modified residue	795	1	Phosphothreonine; by HIPK2 Ref.7
	Modified residue	797	1	Phosphothreonine; by HIPK2 Ref.7
	Modified residue	983	1	Phosphothreonine; by HIPK2 Ref.7
Natural variations
	Natural variant	539	1	A → V. Corresponds to variant rs35231078 [ dbSNP | Ensembl ].		VAR_052913
	Natural variant	550	1	M → I. Corresponds to variant rs871990 [ dbSNP | Ensembl ].		VAR_018383
	Natural variant	561	1	N → S. Corresponds to variant rs34914463 [ dbSNP | Ensembl ].		VAR_052914
Experimental info
	Mutagenesis	795	1	T → A: Impaired HIPK2-mediated phosphorylation; when associated with A-797 and A-983. Ref.7
	Mutagenesis	797	1	T → A: Impaired HIPK2-mediated phosphorylation; when associated with A-795 and A-983. Ref.7
	Mutagenesis	983	1	T → A: Impaired HIPK2-mediated phosphorylation; when associated with A-795 and A-797. Ref.7
	Sequence conflict	188	1	P → S in AAH43352. Ref.6
	Sequence conflict	674	1	R → C in AAH43352. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9P1Z0 [UniParc]. Last modified April 26, 2004. Version 3. Checksum: CA4AB6A5230C2F52	FASTA	1,013	105,114
	10 20 30 40 50 60 MPPPAEVTDP SHAPAVLRQL NEQRLRGLFC DVTLIAGDTK FPAHRSVLAA SSPFFREALL 70 80 90 100 110 120 TSAPLPLPPA TGGAAPNPAT TTAASSSSSS SSSSSSSSSS ASSSSSSSSS SPPPASPPAS 130 140 150 160 170 180 SPPRVLELPG VPAAAFSDVL NFIYSARLAL PGGGGDGAAV AEIGALGRRL GISRLQGLGE 190 200 210 220 230 240 GGDAWVPPTP APMATSQPEE DSFGPGPRPA GEWEGDRAEA QAPDLQCSLP RRPLPCPQCG 250 260 270 280 290 300 KSFIHPKRLQ THEAQCRRGA STRGSTGLGA GGAGPGGPAG VDASALPPPV GFRGGPEHVV 310 320 330 340 350 360 KVVGGHVLYV CAACERSYVT LSSLKRHSNV HSWRRKYPCR YCEKVFALAE YRTKHEVWHT 370 380 390 400 410 420 GERRYQCIFC WETFVTYYNL KTHQRAFHGI SPGLLASEKT PNGGYKPKLN TLKLYRLLPM 430 440 450 460 470 480 RAAKRPYKTY SQGAPEAPLS PTLNTPAPVA MPASPPPGPP PAPEPGPPPS VITFAHPAPS 490 500 510 520 530 540 VIVHGGSSSG GGGSGTASTG GSQAASVITY TAPPRPPKKR EYPPPPPEPA ATPTSPATAV 550 560 570 580 590 600 SPATAAGPAM ATTTEEAKGR NPRAGRTLTY TAKPVGGIGG GGGPPTGAGR GPSQLQAPPP 610 620 630 640 650 660 LCQITVRIGE EAIVKRRISE TDLRPGELSG EEMEESEEDE EEEDEEEEEE DEEESKAGGE 670 680 690 700 710 720 DQLWRPYYSY KPKRKAGAAG GASVGGSGLP RGRRPPRWRQ KLERRSWEET PAAESPAGRA 730 740 750 760 770 780 RTERRHRCGD CAQTFTTLRK LRKHQEAHGG GSHSSRAGRR PSTRFTCPHC AKVCKTAAAL 790 800 810 820 830 840 SRHGQRHAAE RPGGTPTPVI AYSKGSAGTR PGDVKEEAPQ EMQVSSSSGE AGGGSTAAEE 850 860 870 880 890 900 ASETASLQDP IISGGEEPPV VASGGSYVYP PVQEFPLALI GGGREPGGGR GKSGSEGPVG 910 920 930 940 950 960 AGEGDRMEGI GAAKVTFYPE PYPLVYGPQL LAAYPYNFSN LAALPVALNM VLPDEKGAGA 970 980 990 1000 1010 LPFLPGVFGY AVNPQAAPPA PPTPPPPTLP PPIPPKGEGE RAGVERTQKG DVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel zinc-finger-protein encoding gene on 17p13.1." Weber A., Klinkhammer B., Glaum A., Bergmann E., Berwanger B., Eilers M., Christiansen H. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:143-150(2000) [PubMed: 10819331] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones." Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T. DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Uterus.
[7]	"The human protein kinase HIPK2 phosphorylates and downregulates the methyl-binding transcription factor ZBTB4." Yamada D., Perez-Torrado R., Filion G., Caly M., Jammart B., Devignot V., Sasai N., Ravassard P., Mallet J., Sastre-Garau X., Schmitz M.L., Defossez P.A. Oncogene 28:2535-2544(2009) [PubMed: 19448668] [Abstract] Cited for: PHOSPHORYLATION AT THR-795; THR-797 AND THR-983 BY HIPK2, MUTAGENESIS OF THR-795; THR-797 AND THR-983, INTERACTION WITH HIPK2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY302699 mRNA. Translation: AAP59447.1. AB040971 mRNA. Translation: BAA96062.2. Different initiation. AK122971 mRNA. Translation: BAG53828.1. CH471108 Genomic DNA. Translation: EAW90185.1. BC033259 mRNA. Translation: AAH33259.1. BC043352 mRNA. Translation: AAH43352.1.
IPI	IPI00001835.
RefSeq	NP_001122305.1. NM_001128833.1. NP_065950.2. NM_020899.3.
UniGene	Hs.35096.
3D structure databases
ProteinModelPortal	Q9P1Z0.
SMR	Q9P1Z0. Positions 2-90, 232-434, 665-797.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P1Z0. 1 interaction.
STRING	Q9P1Z0.
PTM databases
PhosphoSite	Q9P1Z0.
Polymorphism databases
DMDM	46577564.
Proteomic databases
PRIDE	Q9P1Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311403; ENSP00000307858; ENSG00000174282. ENST00000380599; ENSP00000369973; ENSG00000174282.
GeneID	57659.
KEGG	hsa:57659.
UCSC	uc002ghc.2. human.
Organism-specific databases
CTD	57659.
GeneCards	GC17M007362.
H-InvDB	HIX0013496.
HGNC	HGNC:23847. ZBTB4.
HPA	CAB008980.
MIM	612308. gene.
neXtProt	NX_Q9P1Z0.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13694.
GeneTree	ENSGT00530000063209.
HOGENOM	HBG755359.
HOVERGEN	HBG055508.
InParanoid	Q9P1Z0.
OMA	ALSRHGQ.
OrthoDB	EOG4P8FJ3.
PhylomeDB	Q9P1Z0.
Gene expression databases
ArrayExpress	Q9P1Z0.
Bgee	Q9P1Z0.
CleanEx	HS_ZBTB4.
Genevestigator	Q9P1Z0.
GermOnline	ENSG00000174282. Homo sapiens.
Family and domain databases
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.710.10. BTB/POZ_fold. 2 hits. G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 3 hits.
KO	K10491.
Pfam	PF00651. BTB. 1 hit. PF00096. zf-C2H2. 5 hits. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 6 hits. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 5 hits. PS50157. ZINC_FINGER_C2H2_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	64421.
SOURCE	Search...

Entry information

Entry name

ZBTB4_HUMAN

Accession

Primary (citable) accession number: Q9P1Z0
Secondary accession number(s): B3KVL6 , Q7Z697, Q86XJ4, Q8N4V8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	January 25, 2012
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.