Q9P1W8 (SIRPG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal-regulatory protein gamma
Short name=SIRP-gamma

Alternative name(s):
CD172 antigen-like family member B
Signal-regulatory protein beta-2
Short name=SIRP-b2
Short name=SIRP-beta-2
CD_antigen=CD172g

Gene names

Name:	SIRPG
Synonyms:	SIRPB2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Ref.2
Subunit structure	Interacts with CD47. Ref.2
Subcellular location	Membrane; Single-pass type I membrane protein Ref.2.
Tissue specificity	Detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. Mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection. Ref.1 Ref.2
Sequence similarities	Contains 2 Ig-like C1-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW cell-cell signaling Traceable author statement PubMed 9062191. Source: ProtInc intracellular signal transduction Traceable author statement PubMed 9062191. Source: ProtInc leukocyte migration Traceable author statement. Source: Reactome negative regulation of cell proliferation Traceable author statement PubMed 9062191. Source: ProtInc positive regulation of T cell activation Inferred from direct assay Ref.2. Source: UniProtKB positive regulation of cell proliferation Inferred from direct assay Ref.2. Source: UniProtKB positive regulation of cell-cell adhesion Inferred from direct assay Ref.2. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CD47	Q08722	2	EBI-1268284,EBI-1268321

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P1W8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P1W8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9P1W8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     144-360: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9P1W8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     250-360: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Potential

Chain

29 – 387

359

Signal-regulatory protein gamma

PRO_0000014957

Regions

Topological domain

29 – 360

332

Extracellular Potential

Transmembrane

361 – 383

23

Helical; Potential

Topological domain

384 – 387

4

Cytoplasmic Potential

Domain

29 – 137

109

Ig-like V-type

Domain

146 – 245

100

Ig-like C1-type 1

Domain

252 – 340

89

Ig-like C1-type 2

Amino acid modifications

Glycosylation

243

1

N-linked (GlcNAc...) Ref.6

Glycosylation

268

1

N-linked (GlcNAc...) Potential

Glycosylation

309

1

N-linked (GlcNAc...) Potential

Glycosylation

317

1

N-linked (GlcNAc...) Potential

Disulfide bond

53 ↔ 119

Ref.7

Disulfide bond

168 ↔ 226

Potential

Disulfide bond

271 ↔ 329

Potential

Natural variations

Alternative sequence

1 – 33

33

Missing in isoform 2.

VSP_007027

Alternative sequence

144 – 360

217

Missing in isoform 3.

VSP_007028

Alternative sequence

250 – 360

111

Missing in isoform 4.

VSP_026960

Natural variant

263

1

V → A. Ref.1
Corresponds to variant rs6043409 [ dbSNP | Ensembl ].

VAR_049936

Natural variant

286

1

S → L. Ref.1
Corresponds to variant rs6034239 [ dbSNP | Ensembl ].

VAR_049937

Secondary structure

1

.

.

.

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.

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.

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387

Helix Strand Turn

Details...

Beta strand

39 – 43

5

Beta strand

49 – 51

3

Beta strand

54 – 56

3

Beta strand

64 – 71

8

Beta strand

75 – 82

8

Beta strand

88 – 92

5

Beta strand

104 – 106

3

Helix

111 – 113

3

Beta strand

115 – 123

9

Beta strand

130 – 134

5

Beta strand

138 – 142

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0F5099BE1DE35AC8	FASTA	387	42,498
	10 20 30 40 50 60 MPVPASWPHP PGPFLLLTLL LGLTEVAGEE ELQMIQPEKL LLVTVGKTAT LHCTVTSLLP 70 80 90 100 110 120 VGPVLWFRGV GPGRELIYNQ KEGHFPRVTT VSDLTKRNNM DFSIRISSIT PADVGTYYCV 130 140 150 160 170 180 KFRKGSPENV EFKSGPGTEM ALGAKPSAPV VLGPAARTTP EHTVSFTCES HGFSPRDITL 190 200 210 220 230 240 KWFKNGNELS DFQTNVDPTG QSVAYSIRST ARVVLDPWDV RSQVICEVAH VTLQGDPLRG 250 260 270 280 290 300 TANLSEAIRV PPTLEVTQQP MRVGNQVNVT CQVRKFYPQS LQLTWSENGN VCQRETASTL 310 320 330 340 350 360 TENKDGTYNW TSWFLVNISD QRDDVVLTCQ VKHDGQLAVS KRLALEVTVH QKDQSSDATP 370 380 GPASSLTALL LIAVLLGPIY VPWKQKT
	Isoform 2 [UniParc]. Checksum: 41D5122588FE7C42	FASTA	354	38,962
	10 20 30 40 50 60 MIQPEKLLLV TVGKTATLHC TVTSLLPVGP VLWFRGVGPG RELIYNQKEG HFPRVTTVSD 70 80 90 100 110 120 LTKRNNMDFS IRISSITPAD VGTYYCVKFR KGSPENVEFK SGPGTEMALG AKPSAPVVLG 130 140 150 160 170 180 PAARTTPEHT VSFTCESHGF SPRDITLKWF KNGNELSDFQ TNVDPTGQSV AYSIRSTARV 190 200 210 220 230 240 VLDPWDVRSQ VICEVAHVTL QGDPLRGTAN LSEAIRVPPT LEVTQQPMRV GNQVNVTCQV 250 260 270 280 290 300 RKFYPQSLQL TWSENGNVCQ RETASTLTEN KDGTYNWTSW FLVNISDQRD DVVLTCQVKH 310 320 330 340 350 DGQLAVSKRL ALEVTVHQKD QSSDATPGPA SSLTALLLIA VLLGPIYVPW KQKT
	Isoform 3 [UniParc]. Checksum: 0439DB85CAAB167A	FASTA	170	18,466
	10 20 30 40 50 60 MPVPASWPHP PGPFLLLTLL LGLTEVAGEE ELQMIQPEKL LLVTVGKTAT LHCTVTSLLP 70 80 90 100 110 120 VGPVLWFRGV GPGRELIYNQ KEGHFPRVTT VSDLTKRNNM DFSIRISSIT PADVGTYYCV 130 140 150 160 170 KFRKGSPENV EFKSGPGTEM ALGGPASSLT ALLLIAVLLG PIYVPWKQKT
	Isoform 4 [UniParc]. Checksum: AF76A8692051D6C1	FASTA	276	29,967
	10 20 30 40 50 60 MPVPASWPHP PGPFLLLTLL LGLTEVAGEE ELQMIQPEKL LLVTVGKTAT LHCTVTSLLP 70 80 90 100 110 120 VGPVLWFRGV GPGRELIYNQ KEGHFPRVTT VSDLTKRNNM DFSIRISSIT PADVGTYYCV 130 140 150 160 170 180 KFRKGSPENV EFKSGPGTEM ALGAKPSAPV VLGPAARTTP EHTVSFTCES HGFSPRDITL 190 200 210 220 230 240 KWFKNGNELS DFQTNVDPTG QSVAYSIRST ARVVLDPWDV RSQVICEVAH VTLQGDPLRG 250 260 270 TANLSEAIRG PASSLTALLL IAVLLGPIYV PWKQKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel human gene (SIRP-B2) which encodes a new member of the SIRP/SHPS-1 protein family." Ichigotani Y., Matsuda S., Machida K., Oshima K., Iwamoto T., Yamaki K., Hayakawa T., Hamaguchi M. J. Hum. Genet. 45:378-382(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANTS ALA-263 AND LEU-286. Tissue: Placenta.
[2]	"Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation." Piccio L., Vermi W., Boles K.S., Fuchs A., Strader C.A., Facchetti F., Cella M., Colonna M. Blood 105:2421-2427(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, INTERACTION WITH CD47, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: T-cell.
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Lung and Ovary.
[6]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-243, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[7]	"Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47." Hatherley D., Graham S.C., Turner J., Harlos K., Stuart D.I., Barclay A.N. Mol. Cell 31:266-277(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 29-147, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB042624 mRNA. Translation: BAA95692.1.
AY748247 mRNA. Translation: AAV88530.1.
AY748248 mRNA. Translation: AAV88531.1.
AL109809, AL138804 Genomic DNA. Translation: CAM28283.1.
AL109809, AL138804 Genomic DNA. Translation: CAM28284.1.
AL109809, AL138804 Genomic DNA. Translation: CAM28285.1.
CH471133 Genomic DNA. Translation: EAX10616.1.
BC020629 mRNA. Translation: AAH20629.2.
BC064532 mRNA. Translation: AAH64532.1.

IPI

IPI00032061.
IPI00218601.
IPI00550753.
IPI00783247.

RefSeq

NP_001034597.1. NM_001039508.1.
NP_061026.2. NM_018556.3.
NP_543006.2. NM_080816.2.

UniGene

Hs.590883.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JJW	X-ray	1.70	A	29-147	[»]

ProteinModelPortal

Q9P1W8.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9P1W8. 1 interaction.

STRING

9606.ENSP00000305529.

PTM databases

PhosphoSite

Q9P1W8.

Polymorphism databases

DMDM

124053651.

Proteomic databases

PaxDb

Q9P1W8.

PRIDE

Q9P1W8.

Protocols and materials databases

DNASU

55423.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000216927; ENSP00000216927; ENSG00000089012.
ENST00000303415; ENSP00000305529; ENSG00000089012.
ENST00000344103; ENSP00000342759; ENSG00000089012.
ENST00000381580; ENSP00000370992; ENSG00000089012.
ENST00000381583; ENSP00000370995; ENSG00000089012.

GeneID

55423.

KEGG

hsa:55423.

UCSC

uc002wfm.1. human.
uc002wfn.1. human.
uc002wfo.1. human.

Organism-specific databases

CTD

55423.

GeneCards

GC20M001558.

HGNC

HGNC:15757. SIRPG.

MIM

605466. gene.

neXtProt

NX_Q9P1W8.

PharmGKB

PA38034.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG291387.

HOVERGEN

HBG056632.

InParanoid

Q9P1W8.

KO

K06551.

OMA

CITRNES.

PhylomeDB

Q9P1W8.

Enzyme and pathway databases

Reactome

REACT_111155. Cell-Cell communication.
REACT_604. Hemostasis.

Gene expression databases

Bgee

Q9P1W8.

CleanEx

HS_SIRPG.

Genevestigator

Q9P1W8.

GermOnline

ENSG00000089012. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 3 hits.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]

Pfam

PF07654. C1-set. 2 hits.
PF07686. V-set. 1 hit.
[Graphical view]

SMART

SM00409. IG. 1 hit.
SM00407. IGc1. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9P1W8.

GenomeRNAi

55423.

NextBio

59818.

SOURCE

Search...

Entry information

Entry name

SIRPG_HUMAN

Accession

Primary (citable) accession number: Q9P1W8
Secondary accession number(s): B1AKP6 , Q5D051, Q5JV25, Q5MKL4, Q8WWA5, Q9NQK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.