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Q9P1W8 (SIRPG_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal-regulatory protein gamma

Short name=SIRP-gamma
Alternative name(s):
CD172 antigen-like family member B
Signal-regulatory protein beta-2
Short name=SIRP-b2
Short name=SIRP-beta-2
CD_antigen=CD172g
Gene names
Name:SIRPG
Synonyms:SIRPB2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Ref.2

Subunit structure

Interacts with CD47. Ref.2

Subcellular location

Membrane; Single-pass type I membrane protein Ref.2.

Tissue specificity

Detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. Mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection. Ref.1 Ref.2

Sequence similarities

Contains 2 Ig-like C1-type (immunoglobulin-like) domains.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CD47Q087222EBI-1268284,EBI-1268321

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P1W8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P1W8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9P1W8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     144-360: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q9P1W8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     250-360: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 387359Signal-regulatory protein gamma
PRO_0000014957

Regions

Topological domain29 – 360332Extracellular Potential
Transmembrane361 – 38323Helical; Potential
Topological domain384 – 3874Cytoplasmic Potential
Domain29 – 137109Ig-like V-type
Domain146 – 245100Ig-like C1-type 1
Domain252 – 34089Ig-like C1-type 2

Amino acid modifications

Glycosylation2431N-linked (GlcNAc...) Ref.6
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation3171N-linked (GlcNAc...) Potential
Disulfide bond53 ↔ 119 Ref.7
Disulfide bond168 ↔ 226 Potential
Disulfide bond271 ↔ 329 Potential

Natural variations

Alternative sequence1 – 3333Missing in isoform 2.
VSP_007027
Alternative sequence144 – 360217Missing in isoform 3.
VSP_007028
Alternative sequence250 – 360111Missing in isoform 4.
VSP_026960
Natural variant2631V → A. Ref.1
Corresponds to variant rs6043409 [ dbSNP | Ensembl ].
VAR_049936
Natural variant2861S → L. Ref.1
Corresponds to variant rs6034239 [ dbSNP | Ensembl ].
VAR_049937

Secondary structure

........................................................ 387
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 0F5099BE1DE35AC8

FASTA38742,498
        10         20         30         40         50         60 
MPVPASWPHP PGPFLLLTLL LGLTEVAGEE ELQMIQPEKL LLVTVGKTAT LHCTVTSLLP 

        70         80         90        100        110        120 
VGPVLWFRGV GPGRELIYNQ KEGHFPRVTT VSDLTKRNNM DFSIRISSIT PADVGTYYCV 

       130        140        150        160        170        180 
KFRKGSPENV EFKSGPGTEM ALGAKPSAPV VLGPAARTTP EHTVSFTCES HGFSPRDITL 

       190        200        210        220        230        240 
KWFKNGNELS DFQTNVDPTG QSVAYSIRST ARVVLDPWDV RSQVICEVAH VTLQGDPLRG 

       250        260        270        280        290        300 
TANLSEAIRV PPTLEVTQQP MRVGNQVNVT CQVRKFYPQS LQLTWSENGN VCQRETASTL 

       310        320        330        340        350        360 
TENKDGTYNW TSWFLVNISD QRDDVVLTCQ VKHDGQLAVS KRLALEVTVH QKDQSSDATP 

       370        380 
GPASSLTALL LIAVLLGPIY VPWKQKT 

« Hide

Isoform 2 [UniParc].

Checksum: 41D5122588FE7C42
Show »

FASTA35438,962
Isoform 3 [UniParc].

Checksum: 0439DB85CAAB167A
Show »

FASTA17018,466
Isoform 4 [UniParc].

Checksum: AF76A8692051D6C1
Show »

FASTA27629,967

References

« Hide 'large scale' references
[1]"Molecular cloning of a novel human gene (SIRP-B2) which encodes a new member of the SIRP/SHPS-1 protein family."
Ichigotani Y., Matsuda S., Machida K., Oshima K., Iwamoto T., Yamaki K., Hayakawa T., Hamaguchi M.
J. Hum. Genet. 45:378-382(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, VARIANTS ALA-263 AND LEU-286.
Tissue: Placenta.
[2]"Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation."
Piccio L., Vermi W., Boles K.S., Fuchs A., Strader C.A., Facchetti F., Cella M., Colonna M.
Blood 105:2421-2427(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), FUNCTION, INTERACTION WITH CD47, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: T-cell.
[3]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Lung and Ovary.
[6]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-243.
Tissue: Leukemic T-cell.
[7]"Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47."
Hatherley D., Graham S.C., Turner J., Harlos K., Stuart D.I., Barclay A.N.
Mol. Cell 31:266-277(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 29-147, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB042624 mRNA. Translation: BAA95692.1.
AY748247 mRNA. Translation: AAV88530.1.
AY748248 mRNA. Translation: AAV88531.1.
AL109809, AL138804 Genomic DNA. Translation: CAM28283.1.
AL109809, AL138804 Genomic DNA. Translation: CAM28284.1.
AL109809, AL138804 Genomic DNA. Translation: CAM28285.1.
CH471133 Genomic DNA. Translation: EAX10616.1.
BC020629 mRNA. Translation: AAH20629.2.
BC064532 mRNA. Translation: AAH64532.1.
CCDSCCDS13020.2. [Q9P1W8-1]
CCDS13021.2. [Q9P1W8-3]
CCDS33434.1. [Q9P1W8-4]
RefSeqNP_001034597.1. NM_001039508.1. [Q9P1W8-4]
NP_061026.2. NM_018556.3. [Q9P1W8-1]
NP_543006.2. NM_080816.2. [Q9P1W8-3]
UniGeneHs.590883.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JJWX-ray1.70A29-147[»]
4I2XX-ray2.48E/F29-347[»]
ProteinModelPortalQ9P1W8.
SMRQ9P1W8. Positions 30-345.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120664. 1 interaction.
IntActQ9P1W8. 1 interaction.
STRING9606.ENSP00000305529.

PTM databases

PhosphoSiteQ9P1W8.

Polymorphism databases

DMDM124053651.

Proteomic databases

MaxQBQ9P1W8.
PaxDbQ9P1W8.
PRIDEQ9P1W8.

Protocols and materials databases

DNASU55423.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000216927; ENSP00000216927; ENSG00000089012. [Q9P1W8-4]
ENST00000303415; ENSP00000305529; ENSG00000089012. [Q9P1W8-1]
ENST00000344103; ENSP00000342759; ENSG00000089012. [Q9P1W8-3]
ENST00000381580; ENSP00000370992; ENSG00000089012. [Q9P1W8-2]
ENST00000381583; ENSP00000370995; ENSG00000089012. [Q9P1W8-4]
GeneID55423.
KEGGhsa:55423.
UCSCuc002wfm.1. human. [Q9P1W8-1]
uc002wfn.1. human. [Q9P1W8-4]
uc002wfo.1. human. [Q9P1W8-3]

Organism-specific databases

CTD55423.
GeneCardsGC20M001558.
HGNCHGNC:15757. SIRPG.
MIM605466. gene.
neXtProtNX_Q9P1W8.
PharmGKBPA38034.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG291387.
HOVERGENHBG056632.
InParanoidQ9P1W8.
KOK06551.
OMAELISITW.
OrthoDBEOG7CG6ZQ.
PhylomeDBQ9P1W8.
TreeFamTF341862.

Enzyme and pathway databases

ReactomeREACT_111155. Cell-Cell communication.
REACT_604. Hemostasis.

Gene expression databases

BgeeQ9P1W8.
CleanExHS_SIRPG.
GenevestigatorQ9P1W8.

Family and domain databases

Gene3D2.60.40.10. 3 hits.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF07654. C1-set. 2 hits.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00407. IGc1. 2 hits.
[Graphical view]
PROSITEPS50835. IG_LIKE. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9P1W8.
GeneWikiSIRPG.
GenomeRNAi55423.
NextBio59818.
PROQ9P1W8.
SOURCESearch...

Entry information

Entry nameSIRPG_HUMAN
AccessionPrimary (citable) accession number: Q9P1W8
Secondary accession number(s): B1AKP6 expand/collapse secondary AC list , Q5D051, Q5JV25, Q5MKL4, Q8WWA5, Q9NQK8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries