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Q9P1U1 (ARP3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-related protein 3B
Alternative name(s):
ARP3-beta
Actin-like protein 3B
Actin-related protein ARP4
Gene names
Name:ACTR3B
Synonyms:ARP11, ARP4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a role in the organization of the actin cytoskeleton. May function as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. May decrease the metastatic potential of tumors. Ref.7

Subunit structure

Interacts with the Arp2/3 complex composed of ARP2, ARP3, ARPC1B, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

Subcellular location

Cytoplasmcytoskeleton By similarity. Cell projection By similarity.

Tissue specificity

Detected in fetal brain. Detected throughout the adult brain, in neurons from gray matter, but not in white matter. Detected in liver, skeletal muscle and pancreas. Detected in lung adenocarcinoma cells with low metastatic potential, but not in lung adenocarcinoma cells with high metastatic potential. Ref.1 Ref.6

Sequence similarities

Belongs to the actin family. ARP3 subfamily.

Sequence caution

The sequence AAP97150.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
   Cellular componentCell projection
Cytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
Polymorphism
   LigandActin-binding
ATP-binding
Nucleotide-binding
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcell projection

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P1U1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P1U1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-88: Missing.
Isoform 3 (identifier: Q9P1U1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     318-387: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 418417Actin-related protein 3B
PRO_0000342358

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue2401N6-acetyllysine By similarity
Modified residue2441N6-acetyllysine By similarity
Modified residue2511N6-acetyllysine By similarity
Modified residue2541N6-acetyllysine By similarity

Natural variations

Alternative sequence2 – 8887Missing in isoform 2.
VSP_034418
Alternative sequence318 – 38770Missing in isoform 3.
VSP_041474
Natural variant2501R → Q.
Corresponds to variant rs2260545 [ dbSNP | Ensembl ].
VAR_048188

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: BC1040ED5D3B832F

FASTA41847,608
        10         20         30         40         50         60 
MAGSLPPCVV DCGTGYTKLG YAGNTEPQFI IPSCIAIRES AKVVDQAQRR VLRGVDDLDF 

        70         80         90        100        110        120 
FIGDEAIDKP TYATKWPIRH GIIEDWDLME RFMEQVVFKY LRAEPEDHYF LMTEPPLNTP 

       130        140        150        160        170        180 
ENREYLAEIM FESFNVPGLY IAVQAVLALA ASWTSRQVGE RTLTGIVIDS GDGVTHVIPV 

       190        200        210        220        230        240 
AEGYVIGSCI KHIPIAGRDI TYFIQQLLRE REVGIPPEQS LETAKAIKEK YCYICPDIVK 

       250        260        270        280        290        300 
EFAKYDVDPR KWIKQYTGIN AINQKKFVID VGYERFLGPE IFFHPEFANP DFMESISDVV 

       310        320        330        340        350        360 
DEVIQNCPID VRRPLYKNVV LSGGSTMFRD FGRRLQRDLK RVVDARLRLS EELSGGRIKP 

       370        380        390        400        410 
KPVEVQVVTH HMQRYAVWFG GSMLASTPEF FQVCHTKKDY EEYGPSICRH NPVFGVMS 

« Hide

Isoform 2 [UniParc].

Checksum: 0FF9348637DC9879
Show »

FASTA33138,031
Isoform 3 [UniParc].

Checksum: B9EC2A327C56C6CB
Show »

FASTA34839,698

References

« Hide 'large scale' references
[1]"ARP3beta, the gene encoding a new human actin-related protein, is alternatively spliced and predominantly expressed in brain neuronal cells."
Jay P., Berge-Lefranc J.-L., Massacrier A., Roessler E., Wallis D., Muenke M., Gastaldi M., Taviaux S., Cau P., Berta P.
Eur. J. Biochem. 267:2921-2928(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Embryo.
[2]"Cloning of a novel human cDNA homology to human actin-related protein Arp3(ARP3) mRNA."
Ding J.B., Yu L., Zhang M., Dai F.Y., Jiang J.X., Zhao S.Y.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Amygdala and Peripheral blood.
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Colon and Skin.
[6]"Isolation of a novel actin-related gene expressed in low-metastatic PC-14 human lung adenocarcinoma."
Shindo-Okada N., Shimizu K.
Biochem. Biophys. Res. Commun. 280:61-67(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Expression of the Arp11 gene suppresses the tumorigenicity of PC-14 human lung adenocarcinoma cells."
Shindo-Okada N., Iigo M.
Biochem. Biophys. Res. Commun. 312:889-896(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF023453 mRNA. Translation: AAC98904.1.
AF086920 mRNA. Translation: AAP97150.1. Sequence problems.
AK294292 mRNA. Translation: BAG57575.1.
AK315534 mRNA. Translation: BAG37914.1.
AC072057 Genomic DNA. No translation available.
AC092180 Genomic DNA. No translation available.
BC015207 mRNA. Translation: AAH15207.1.
BC008682 mRNA. Translation: AAH08682.1.
CCDSCCDS34782.1. [Q9P1U1-3]
CCDS5934.1. [Q9P1U1-1]
RefSeqNP_001035225.1. NM_001040135.2. [Q9P1U1-3]
NP_065178.1. NM_020445.5. [Q9P1U1-1]
UniGeneHs.647117.

3D structure databases

ProteinModelPortalQ9P1U1.
SMRQ9P1U1. Positions 3-417.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121429. 15 interactions.
IntActQ9P1U1. 3 interactions.
MINTMINT-1397170.
STRING9606.ENSP00000256001.

PTM databases

PhosphoSiteQ9P1U1.

Polymorphism databases

DMDM74753111.

Proteomic databases

MaxQBQ9P1U1.
PaxDbQ9P1U1.
PRIDEQ9P1U1.

Protocols and materials databases

DNASU57180.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000256001; ENSP00000256001; ENSG00000133627. [Q9P1U1-1]
ENST00000377776; ENSP00000367007; ENSG00000133627. [Q9P1U1-3]
ENST00000397282; ENSP00000380452; ENSG00000133627. [Q9P1U1-2]
ENST00000537264; ENSP00000446157; ENSG00000133627. [Q9P1U1-2]
GeneID57180.
KEGGhsa:57180.
UCSCuc003wle.2. human. [Q9P1U1-1]
uc003wlf.2. human. [Q9P1U1-3]

Organism-specific databases

CTD57180.
GeneCardsGC07P152456.
HGNCHGNC:17256. ACTR3B.
neXtProtNX_Q9P1U1.
PharmGKBPA142672645.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5277.
HOGENOMHOG000233339.
HOVERGENHBG003771.
InParanoidQ9P1U1.
OMAGDPRKWI.
OrthoDBEOG7TMZRM.
PhylomeDBQ9P1U1.
TreeFamTF300644.

Enzyme and pathway databases

SignaLinkQ9P1U1.

Gene expression databases

ArrayExpressQ9P1U1.
BgeeQ9P1U1.
CleanExHS_ACTR3B.
GenevestigatorQ9P1U1.

Family and domain databases

InterProIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
[Graphical view]
PANTHERPTHR11937. PTHR11937. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiACTR3B.
GenomeRNAi57180.
NextBio63219.
PROQ9P1U1.

Entry information

Entry nameARP3B_HUMAN
AccessionPrimary (citable) accession number: Q9P1U1
Secondary accession number(s): A8MTG1 expand/collapse secondary AC list , B4DFW4, Q7Z526, Q96BT2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM