Q9P1U1 (ARP3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin-related protein 3B

Alternative name(s):
ARP3-beta
Actin-like protein 3B
Actin-related protein ARP4

Gene names

Name:	ACTR3B
Synonyms:	ARP11, ARP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the organization of the actin cytoskeleton. May function as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. May decrease the metastatic potential of tumors. Ref.7
Subunit structure	Interacts with the Arp2/3 complex composed of ARP2, ARP3, ARPC1B, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC. Ref.8
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cell projection By similarity.
Tissue specificity	Detected in fetal brain. Detected throughout the adult brain, in neurons from gray matter, but not in white matter. Detected in liver, skeletal muscle and pancreas. Detected in lung adenocarcinoma cells with low metastatic potential, but not in lung adenocarcinoma cells with high metastatic potential. Ref.1 Ref.6
Sequence similarities	Belongs to the actin family. ARP3 subfamily.
Sequence caution	The sequence AAP97150.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Actin-binding Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of actin filament polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	cell projection Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P1U1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P1U1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: Missing.

Isoform 3 (identifier: Q9P1U1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     318-387: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 418	417	Actin-related protein 3B		PRO_0000342358
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	231	1	Phosphotyrosine By similarity
	Modified residue	418	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 88	88	Missing in isoform 2.		VSP_034418
	Alternative sequence	318 – 387	70	Missing in isoform 3.		VSP_041474
	Natural variant	250	1	R → Q. Corresponds to variant rs2260545 [ dbSNP | Ensembl ].		VAR_048188

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BC1040ED5D3B832F	FASTA	418	47,608
	10 20 30 40 50 60 MAGSLPPCVV DCGTGYTKLG YAGNTEPQFI IPSCIAIRES AKVVDQAQRR VLRGVDDLDF 70 80 90 100 110 120 FIGDEAIDKP TYATKWPIRH GIIEDWDLME RFMEQVVFKY LRAEPEDHYF LMTEPPLNTP 130 140 150 160 170 180 ENREYLAEIM FESFNVPGLY IAVQAVLALA ASWTSRQVGE RTLTGIVIDS GDGVTHVIPV 190 200 210 220 230 240 AEGYVIGSCI KHIPIAGRDI TYFIQQLLRE REVGIPPEQS LETAKAIKEK YCYICPDIVK 250 260 270 280 290 300 EFAKYDVDPR KWIKQYTGIN AINQKKFVID VGYERFLGPE IFFHPEFANP DFMESISDVV 310 320 330 340 350 360 DEVIQNCPID VRRPLYKNVV LSGGSTMFRD FGRRLQRDLK RVVDARLRLS EELSGGRIKP 370 380 390 400 410 KPVEVQVVTH HMQRYAVWFG GSMLASTPEF FQVCHTKKDY EEYGPSICRH NPVFGVMS
	Isoform 2 [UniParc]. Checksum: 3E9370B5EA5742A0	FASTA	330	37,900
	10 20 30 40 50 60 MERFMEQVVF KYLRAEPEDH YFLMTEPPLN TPENREYLAE IMFESFNVPG LYIAVQAVLA 70 80 90 100 110 120 LAASWTSRQV GERTLTGIVI DSGDGVTHVI PVAEGYVIGS CIKHIPIAGR DITYFIQQLL 130 140 150 160 170 180 REREVGIPPE QSLETAKAIK EKYCYICPDI VKEFAKYDVD PRKWIKQYTG INAINQKKFV 190 200 210 220 230 240 IDVGYERFLG PEIFFHPEFA NPDFMESISD VVDEVIQNCP IDVRRPLYKN VVLSGGSTMF 250 260 270 280 290 300 RDFGRRLQRD LKRVVDARLR LSEELSGGRI KPKPVEVQVV THHMQRYAVW FGGSMLASTP 310 320 330 EFFQVCHTKK DYEEYGPSIC RHNPVFGVMS
	Isoform 3 [UniParc]. Checksum: B9EC2A327C56C6CB	FASTA	348	39,698
	10 20 30 40 50 60 MAGSLPPCVV DCGTGYTKLG YAGNTEPQFI IPSCIAIRES AKVVDQAQRR VLRGVDDLDF 70 80 90 100 110 120 FIGDEAIDKP TYATKWPIRH GIIEDWDLME RFMEQVVFKY LRAEPEDHYF LMTEPPLNTP 130 140 150 160 170 180 ENREYLAEIM FESFNVPGLY IAVQAVLALA ASWTSRQVGE RTLTGIVIDS GDGVTHVIPV 190 200 210 220 230 240 AEGYVIGSCI KHIPIAGRDI TYFIQQLLRE REVGIPPEQS LETAKAIKEK YCYICPDIVK 250 260 270 280 290 300 EFAKYDVDPR KWIKQYTGIN AINQKKFVID VGYERFLGPE IFFHPEFANP DFMESISDVV 310 320 330 340 DEVIQNCPID VRRPLYKPEF FQVCHTKKDY EEYGPSICRH NPVFGVMS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ARP3beta, the gene encoding a new human actin-related protein, is alternatively spliced and predominantly expressed in brain neuronal cells." Jay P., Berge-Lefranc J.-L., Massacrier A., Roessler E., Wallis D., Muenke M., Gastaldi M., Taviaux S., Cau P., Berta P. Eur. J. Biochem. 267:2921-2928(2000) [PubMed: 10806390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Tissue: Embryo.
[2]	"Cloning of a novel human cDNA homology to human actin-related protein Arp3(ARP3) mRNA." Ding J.B., Yu L., Zhang M., Dai F.Y., Jiang J.X., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Amygdala and Peripheral blood.
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Colon and Skin.
[6]	"Isolation of a novel actin-related gene expressed in low-metastatic PC-14 human lung adenocarcinoma." Shindo-Okada N., Shimizu K. Biochem. Biophys. Res. Commun. 280:61-67(2001) [PubMed: 11162478] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Expression of the Arp11 gene suppresses the tumorigenicity of PC-14 human lung adenocarcinoma cells." Shindo-Okada N., Iigo M. Biochem. Biophys. Res. Commun. 312:889-896(2003) [PubMed: 14651955] [Abstract] Cited for: FUNCTION.
[8]	"Large-scale mapping of human protein-protein interactions by mass spectrometry." Ewing R.M., Chu P., Elisma F., Li H., Taylor P., Climie S., McBroom-Cerajewski L., Robinson M.D., O'Connor L., Li M., Taylor R., Dharsee M., Ho Y., Heilbut A., Moore L., Zhang S., Ornatsky O., Bukhman Y.V. , Ethier M., Sheng Y., Vasilescu J., Abu-Farha M., Lambert J.-P., Duewel H.S., Stewart I.I., Kuehl B., Hogue K., Colwill K., Gladwish K., Muskat B., Kinach R., Adams S.-L., Moran M.F., Morin G.B., Topaloglou T., Figeys D. Mol. Syst. Biol. 3:89-89(2007) [PubMed: 17353931] [Abstract] Cited for: INTERACTION WITH THE ARP2/3 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF023453 mRNA. Translation: AAC98904.1. AF086920 mRNA. Translation: AAP97150.1. Sequence problems. AK294292 mRNA. Translation: BAG57575.1. AK315534 mRNA. Translation: BAG37914.1. AC072057 Genomic DNA. No translation available. AC092180 Genomic DNA. No translation available. BC015207 mRNA. Translation: AAH15207.1. BC008682 mRNA. Translation: AAH08682.1.
IPI	IPI00007068. IPI00554433. IPI00892652.
RefSeq	NP_001035225.1. NM_001040135.1. NP_065178.1. NM_020445.4.
UniGene	Hs.647117.
3D structure databases
HSSP	HSSP built from PDB template 1U2V based on UniProtKB P61157.
ProteinModelPortal	Q9P1U1.
SMR	Q9P1U1. Positions 3-417.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P1U1. 3 interactions.
MINT	MINT-1397170.
STRING	Q9P1U1.
PTM databases
PhosphoSite	Q9P1U1.
Polymorphism databases
DMDM	74753111.
Proteomic databases
PRIDE	Q9P1U1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000256001; ENSP00000256001; ENSG00000133627. ENST00000377776; ENSP00000367007; ENSG00000133627.
GeneID	57180.
KEGG	hsa:57180.
UCSC	uc003wle.1. human.
Organism-specific databases
CTD	57180.
GeneCards	GC07P152456.
H-InvDB	HIX0007239.
HGNC	HGNC:17256. ACTR3B.
neXtProt	NX_Q9P1U1.
PharmGKB	PA142672645.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074695.
HOGENOM	HBG559892.
HOVERGEN	HBG003771.
InParanoid	Q9P1U1.
OMA	RAWRARC.
OrthoDB	EOG4XKV6W.
PhylomeDB	Q9P1U1.
Gene expression databases
ArrayExpress	Q9P1U1.
Bgee	Q9P1U1.
CleanEx	HS_ACTR3B.
Genevestigator	Q9P1U1.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR020902. Actin/actin-like_CS. IPR015623. Arp3. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit. PTHR11937:SF31. Arp3. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	63219.

Entry information

Entry name

ARP3B_HUMAN

Accession

Primary (citable) accession number: Q9P1U1
Secondary accession number(s): A8MTG1 , B4DFW4, Q7Z526, Q96BT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.