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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4

Gene

GCNT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that mediates core 2 O-glycan branching, an important step in mucin-type biosynthesis. Does not have core 4 O-glycan or I-branching enzyme activity.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.102. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
SignaLinkiQ9P109.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4 (EC:2.4.1.102)
Alternative name(s):
Core 2-branching enzyme 3
Core2-GlcNAc-transferase 3
Short name:
C2GnT3
Gene namesi
Name:GCNT4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:17973. GCNT4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3421Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini35 – 453419LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485475.

Polymorphism and mutation databases

BioMutaiGCNT4.
DMDMi74719783.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 453453Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4PRO_0000288555Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Disulfide bondi73 ↔ 227By similarity
Disulfide bondi161 ↔ 382By similarity
Disulfide bondi182 ↔ 209By similarity
Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence analysis
Disulfide bondi391 ↔ 423By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9P109.
PRIDEiQ9P109.

PTM databases

iPTMnetiQ9P109.
PhosphoSiteiQ9P109.

Expressioni

Tissue specificityi

Predominantly expressed in thymus. Weakly expressed in pancreas, peripheral blood leukocytes, placenta, small intestine and stomach. Barely detectable in liver, spleen, lung and lymph node.1 Publication

Gene expression databases

BgeeiQ9P109.
CleanExiHS_GCNT4.
GenevisibleiQ9P109. HS.

Organism-specific databases

HPAiHPA037431.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000317027.

Structurei

3D structure databases

ProteinModelPortaliQ9P109.
SMRiQ9P109. Positions 71-431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0799. Eukaryota.
ENOG410XQ7M. LUCA.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ9P109.
KOiK09663.
OMAiEAPPHNI.
OrthoDBiEOG74N5HW.
PhylomeDBiQ9P109.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P109-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIFKCYFKH TLQQKVFILF LTLWLLSLLK LLNVRRLFPQ KDIYLVEYSL
60 70 80 90 100
STSPFVRNRY THVKDEVRYE VNCSGIYEQE PLEIGKSLEI RRRDIIDLED
110 120 130 140 150
DDVVAMTSDC DIYQTLRGYA QKLVSKEEKS FPIAYSLVVH KDAIMVERLI
160 170 180 190 200
HAIYNQHNIY CIHYDRKAPD TFKVAMNNLA KCFSNIFIAS KLEAVEYAHI
210 220 230 240 250
SRLQADLNCL SDLLKSSIQW KYVINLCGQD FPLKSNFELV SELKKLNGAN
260 270 280 290 300
MLETVKPPNS KLERFTYHHE LRRVPYEYVK LPIRTNISKE APPHNIQIFV
310 320 330 340 350
GSAYFVLSQA FVKYIFNNSI VQDFFAWSKD TYSPDEHFWA TLIRVPGIPG
360 370 380 390 400
EISRSAQDVS DLQSKTRLVK WNYYEGFFYP SCTGSHLRSV CIYGAAELRW
410 420 430 440 450
LIKDGHWFAN KFDSKVDPIL IKCLAEKLEE QQRDWITLPS EKLFMDRNLT

TTS
Length:453
Mass (Da):53,052
Last modified:October 1, 2000 - v1
Checksum:iB43794D4427F41CA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti321 – 3211V → I.
Corresponds to variant rs4704166 [ dbSNP | Ensembl ].
VAR_032444
Natural varianti321 – 3211V → L.
Corresponds to variant rs4704166 [ dbSNP | Ensembl ].
VAR_061210
Natural varianti439 – 4391P → S.
Corresponds to variant rs3811987 [ dbSNP | Ensembl ].
VAR_032445

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132035 mRNA. Translation: AAF63156.1.
CCDSiCCDS4026.1.
RefSeqiNP_057675.1. NM_016591.2.
UniGeneiHs.272404.

Genome annotation databases

EnsembliENST00000322348; ENSP00000317027; ENSG00000176928.
GeneIDi51301.
KEGGihsa:51301.
UCSCiuc003kdn.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Core 2 beta-1,6-N-acetylglucosaminyltransferase 3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132035 mRNA. Translation: AAF63156.1.
CCDSiCCDS4026.1.
RefSeqiNP_057675.1. NM_016591.2.
UniGeneiHs.272404.

3D structure databases

ProteinModelPortaliQ9P109.
SMRiQ9P109. Positions 71-431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000317027.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

PTM databases

iPTMnetiQ9P109.
PhosphoSiteiQ9P109.

Polymorphism and mutation databases

BioMutaiGCNT4.
DMDMi74719783.

Proteomic databases

PaxDbiQ9P109.
PRIDEiQ9P109.

Protocols and materials databases

DNASUi51301.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322348; ENSP00000317027; ENSG00000176928.
GeneIDi51301.
KEGGihsa:51301.
UCSCiuc003kdn.4. human.

Organism-specific databases

CTDi51301.
GeneCardsiGCNT4.
HGNCiHGNC:17973. GCNT4.
HPAiHPA037431.
neXtProtiNX_Q9P109.
PharmGKBiPA143485475.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0799. Eukaryota.
ENOG410XQ7M. LUCA.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ9P109.
KOiK09663.
OMAiEAPPHNI.
OrthoDBiEOG74N5HW.
PhylomeDBiQ9P109.
TreeFamiTF315534.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.102. 2681.
ReactomeiR-HSA-913709. O-linked glycosylation of mucins.
SignaLinkiQ9P109.

Miscellaneous databases

GenomeRNAii51301.
PROiQ9P109.

Gene expression databases

BgeeiQ9P109.
CleanExiHS_GCNT4.
GenevisibleiQ9P109. HS.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Control of O-glycan branch formation. Molecular cloning and characterization of a novel thymus-associated core 2 beta1, 6-n-acetylglucosaminyltransferase."
    Schwientek T., Yeh J.-C., Levery S.B., Keck B., Merkx G., van Kessel A.G., Fukuda M., Clausen H.
    J. Biol. Chem. 275:11106-11113(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGCNT4_HUMAN
AccessioniPrimary (citable) accession number: Q9P109
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.