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Q9P104 (DOK5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Docking protein 5
Alternative name(s):
Downstream of tyrosine kinase 5
Insulin receptor substrate 6
Short name=IRS-6
Short name=IRS6
Gene names
Name:DOK5
Synonyms:C20orf180
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.

Subunit structure

Interacts with phosphorylated RET. In contrast to other DOK proteins, it does not interact with RASGAP By similarity.

Tissue specificity

Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes. Ref.2 Ref.3

Domain

PTB domain mediates receptor interaction By similarity.

Post-translational modification

Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF. Ref.3

Sequence similarities

Belongs to the DOK family. Type B subfamily.

Contains 1 IRS-type PTB domain.

Contains 1 PH domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P104-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P104-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 306306Docking protein 5
PRO_0000187277

Regions

Domain8 – 112105PH
Domain132 – 237106IRS-type PTB
Motif263 – 27311DKFBH motif

Natural variations

Alternative sequence1 – 108108Missing in isoform 2.
VSP_003854

Experimental info

Sequence conflict225 – 23410SAALAIAEQH → LLQMKMSERA in AAF66443. Ref.1

Secondary structure

................... 306
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: 2F259529E8B068DB

FASTA30635,464
        10         20         30         40         50         60 
MASNFNDIVK QGYVRIRSRR LGIYQRCWLV FKKASSKGPK RLEKFSDERA AYFRCYHKVT 

        70         80         90        100        110        120 
ELNNVKNVAR LPKSTKKHAI GIYFNDDTSK TFACESDLEA DEWCKVLQME CVGTRINDIS 

       130        140        150        160        170        180 
LGEPDLLATG VEREQSERFN VYLMPSPNLD VHGECALQIT YEYICLWDVQ NPRVKLISWP 

       190        200        210        220        230        240 
LSALRRYGRD TTWFTFEAGR MCETGEGLFI FQTRDGEAIY QKVHSAALAI AEQHERLLQS 

       250        260        270        280        290        300 
VKNSMLQMKM SERAASLSTM VPLPRSAYWQ HITRQHSTGQ LYRLQDVSSP LKLHRTETFP 


AYRSEH 

« Hide

Isoform 2 [UniParc].

Checksum: 291F9F2A419FE062
Show »

FASTA19822,824

References

« Hide 'large scale' references
[1]Luo W.Q., Chen J.H., Huang X.W., Zhou Y., Zhou H.J., Hu S.N., Yuan J.G.
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"DOK4 and DOK5: new Dok-related genes expressed in human T cells."
Favre C., Gerard A., Clauzier E., Pontarotti P., Olive D., Nunes J.A.
Genes Immun. 4:40-45(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[3]"Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5."
Cai D., Dhe-Paganon S., Melendez P.A., Lee J., Shoelson S.E.
J. Biol. Chem. 278:25323-25330(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, PHOSPHORYLATION AT TYROSINE RESIDUES.
Tissue: Skeletal muscle.
[4]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[6]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 42-306 (ISOFORM 1).
Tissue: Kidney.
[7]"Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of human dok-5 protein."
Shi N., Zhou W., Tang K., Gao Y., Jin J., Gao F., Peng X., Bartlam M., Qiang B., Yuan J., Rao Z.
Acta Crystallogr. D 58:2170-2172(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF132732 mRNA. Translation: AAF66443.1.
AF466368 mRNA. Translation: AAL74194.1.
AL118501, AL162292 Genomic DNA. Translation: CAI19415.1.
AL118501 Genomic DNA. Translation: CAI19416.1.
AL162292, AL118501 Genomic DNA. Translation: CAI16465.1.
BC008992 mRNA. Translation: AAH08992.1.
AL050069 mRNA. Translation: CAB43255.3.
PIRT08731.
RefSeqNP_060901.2. NM_018431.3.
XP_005260508.1. XM_005260451.1.
UniGeneHs.656582.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1J0WX-ray2.50A/B133-232[»]
ProteinModelPortalQ9P104.
SMRQ9P104. Positions 7-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120926. 4 interactions.
IntActQ9P104. 4 interactions.
MINTMINT-1435904.
STRING9606.ENSP00000262593.

PTM databases

PhosphoSiteQ9P104.

Polymorphism databases

DMDM26393190.

Proteomic databases

PaxDbQ9P104.
PRIDEQ9P104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262593; ENSP00000262593; ENSG00000101134. [Q9P104-1]
ENST00000395939; ENSP00000379270; ENSG00000101134. [Q9P104-2]
GeneID55816.
KEGGhsa:55816.
UCSCuc002xwy.3. human. [Q9P104-1]

Organism-specific databases

CTD55816.
GeneCardsGC20P053092.
HGNCHGNC:16173. DOK5.
MIM608334. gene.
neXtProtNX_Q9P104.
PharmGKBPA25724.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG256436.
HOVERGENHBG002356.
InParanoidQ9P104.
OMAHAVGIYF.
OrthoDBEOG7GTT3W.
PhylomeDBQ9P104.
TreeFamTF324994.

Enzyme and pathway databases

SignaLinkQ9P104.

Gene expression databases

BgeeQ9P104.
CleanExHS_DOK5.
GenevestigatorQ9P104.

Family and domain databases

Gene3D2.30.29.30. 2 hits.
InterProIPR002404. Insln_rcpt_S1.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF02174. IRS. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
[Graphical view]
PROSITEPS51064. IRS_PTB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9P104.
GeneWikiDOK5.
GenomeRNAi55816.
NextBio60998.
PROQ9P104.
SOURCESearch...

Entry information

Entry nameDOK5_HUMAN
AccessionPrimary (citable) accession number: Q9P104
Secondary accession number(s): Q5T7Y0 expand/collapse secondary AC list , Q5TE53, Q8TEW7, Q96H13, Q9BZ24, Q9NQF4, Q9Y411
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: March 19, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM